Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,233 C→T 2.5% P372L (CCG→CTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2330C→T 2.5% 11.5 ‑2.7 ‑0.1314P372L (CCG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (1/7):  ref base (155/151):  total (156/158)
Fisher's exact test strand distribution p-value = 6.69e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.24e-01

Only 100 of 340 total aligned reads displayed.

TCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACG  >  NC_014034/1397133‑1397333
                                                                                                       |                                                                                                      
tCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGcc                                                                                                        >  1:856200/1‑101 (MQ=255)
 ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCg                                                                                                       <  2:6311630/101‑1 (MQ=255)
 ccAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCg                                                                                                       >  2:3080389/1‑101 (MQ=255)
  cAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGt                                                                                                      >  2:1231868/1‑101 (MQ=255)
  cAGCTCGCGCCACATGTCTCCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGt                                                                                                      <  2:6794898/101‑1 (MQ=255)
   aGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTc                                                                                                     <  2:6989829/101‑1 (MQ=255)
    gCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCg                                                                                                    >  1:839979/1‑101 (MQ=255)
      tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCt                                                                                                  >  1:3867637/1‑101 (MQ=255)
      tCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCt                                                                                                  >  1:804873/1‑101 (MQ=255)
       cgcgCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTc                                                                                                 >  1:4032071/1‑101 (MQ=255)
            cacaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTcccccc                                                                                            <  1:1316502/101‑1 (MQ=255)
             acaTGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCcg                                                                                           >  1:2488383/1‑101 (MQ=255)
                tGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCgg                                                                                        >  2:1423945/1‑101 (MQ=255)
                 gTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACGAGATCC.GCGCCGTCGCTCCCCCCGCggg                                                                                       >  1:6177908/1‑101 (MQ=255)
                     tCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCg                                                                                   >  1:4711024/1‑101 (MQ=255)
                      cGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGt                                                                                  <  2:3998883/101‑1 (MQ=255)
                       gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTg                                                                                 >  1:646320/1‑101 (MQ=255)
                       gACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCATg                                                                                 >  2:7085318/1‑101 (MQ=255)
                        gccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                                <  2:2801353/100‑1 (MQ=255)
                        aCCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTgg                                                                                <  1:2734460/101‑1 (MQ=255)
                         ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                               <  2:6442838/101‑1 (MQ=255)
                         ccGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                               <  2:4536227/101‑1 (MQ=255)
                          cGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                              <  1:6714187/101‑1 (MQ=255)
                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             <  1:5022034/101‑1 (MQ=255)
                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             >  1:3027853/1‑101 (MQ=255)
                           gTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.AACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             <  1:6607831/101‑1 (MQ=255)
                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:5745222/101‑1 (MQ=255)
                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  2:1553882/101‑1 (MQ=255)
                            tGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  2:506535/101‑1 (MQ=255)
                              cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  1:3429103/1‑101 (MQ=255)
                                gcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGCTGCTGACACCGCCAACAAA.TATGTGGTCA.ACACTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                         <  1:2851447/100‑1 (MQ=2)
                                  ggcGCTCCCCCCGCGGGGGCGTGGATCCAAACATGGCT..AT.GGCCGCAGTGTCACGTTTTCCAGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAaccc                                                                      <  2:2318788/99‑3 (MQ=2)
                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                      >  2:573725/1‑101 (MQ=255)
                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                      >  2:409453/1‑101 (MQ=255)
                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAcc                                                                     <  2:3918629/101‑1 (MQ=255)
                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACcgc                                                                   <  1:5668394/101‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCa                                                               <  2:2864693/101‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCa                                                               <  2:3810069/101‑1 (MQ=255)
                                        ccccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCa                                                               >  1:1201745/1‑101 (MQ=255)
                                         cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAc                                                              <  2:2056861/101‑1 (MQ=255)
                                         cccccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAc                                                              <  1:3784807/101‑1 (MQ=255)
                                          ctccGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcg                                                             <  2:4600177/99‑1 (MQ=255)
                                             cgcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgcgc                                                          <  2:5674121/101‑1 (MQ=255)
                                              gcgGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgcgcg                                                         <  1:6740378/101‑1 (MQ=255)
                                                gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                       >  2:2151813/1‑101 (MQ=255)
                                                gggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                       >  2:6670077/1‑101 (MQ=255)
                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                      <  1:682326/101‑1 (MQ=255)
                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                      >  2:3765798/1‑101 (MQ=255)
                                                 ggggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                      <  1:4261208/101‑1 (MQ=255)
                                                  gggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACg                                                     <  2:6177589/101‑1 (MQ=255)
                                                   ggCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                    <  2:2967750/101‑1 (MQ=255)
                                                    gCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGc                                                   >  1:4578070/1‑101 (MQ=255)
                                                       tGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGG.GCACGCGCGACCGGC.gcc                                               >  2:3309201/1‑99 (MQ=255)
                                                       tGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                               >  1:1832720/1‑101 (MQ=255)
                                                       tGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCACATCC.GCGCCGACGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                               >  1:697312/1‑101 (MQ=255)
                                                         gATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAg                                             >  1:1692904/1‑101 (MQ=255)
                                                         gATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAg                                             <  2:4134121/101‑1 (MQ=255)
                                                         gATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAg                                             >  2:4839492/1‑101 (MQ=255)
                                                            cGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                          >  1:290781/1‑101 (MQ=255)
                                                               aaCGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgc                                       >  1:2113870/1‑101 (MQ=255)
                                                               aaCGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgc                                       >  1:3066188/1‑101 (MQ=255)
                                                                acctgtCTGCCA.CGACGCCCCG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                      <  1:2269441/98‑1 (MQ=255)
                                                                aCGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                      >  1:3672515/1‑101 (MQ=255)
                                                                  gtgtCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                    >  2:2396017/1‑101 (MQ=255)
                                                                  gtgtCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTACGCCCCGCGaa                                    >  1:3332261/1‑101 (MQ=255)
                                                                   tgtCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                   >  1:6248451/1‑101 (MQ=255)
                                                                     tCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGt                                 <  1:1597091/101‑1 (MQ=255)
                                                                      cTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                <  2:3637329/101‑1 (MQ=255)
                                                                      cTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                >  2:4428407/1‑101 (MQ=255)
                                                                      cTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                <  1:917338/101‑1 (MQ=255)
                                                                       tGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCg                               >  2:3551486/1‑101 (MQ=255)
                                                                          cA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                            >  2:3379763/1‑101 (MQ=255)
                                                                           a.cgacgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                           <  2:4342200/101‑1 (MQ=255)
                                                                           a.cgacgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                           <  2:4191188/101‑1 (MQ=255)
                                                                            ccgacgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                           <  2:2745230/100‑1 (MQ=255)
                                                                             cgacgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                          <  1:4838715/101‑1 (MQ=255)
                                                                              gacgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACACCGC.GCGCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                         <  1:5105902/101‑1 (MQ=255)
                                                                              gacgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                         >  2:6515109/1‑101 (MQ=255)
                                                                               acgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                        >  1:1980130/1‑101 (MQ=255)
                                                                               acgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                        <  1:5503543/101‑1 (MQ=255)
                                                                                cgCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                       <  2:2538734/101‑1 (MQ=255)
                                                                                 gCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                      <  2:1756289/101‑1 (MQ=255)
                                                                                 gCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCcg                      <  1:5371390/101‑1 (MQ=255)
                                                                                      g.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCgggg                 >  2:770308/1‑100 (MQ=255)
                                                                                      g.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCgggg                 >  2:5827817/1‑100 (MQ=255)
                                                                                        cACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                <  2:547647/101‑1 (MQ=255)
                                                                                        cACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAg                >  1:3251026/1‑101 (MQ=255)
                                                                                         aCCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc               >  2:167990/1‑101 (MQ=255)
                                                                                          ccAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg              >  1:361726/1‑101 (MQ=255)
                                                                                            aGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg            <  2:4924313/101‑1 (MQ=255)
                                                                                             gATCC.GCGCCGTGGCGCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg           <  1:7026641/101‑1 (MQ=255)
                                                                                              aTCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa          >  2:4292347/1‑101 (MQ=255)
                                                                                                 c.gcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg       >  1:4646763/1‑101 (MQ=255)
                                                                                                 c.gcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg       <  1:4181082/101‑1 (MQ=255)
                                                                                                    cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa     >  2:135323/1‑101 (MQ=255)
                                                                                                    ccacGTCGCTCCCCCCGCGGGGGCGTGAAGCGAAACAAGGCGGCGCGGGCCTATGGCTGTCGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa     <  2:53700/98‑1 (MQ=2)
                                                                                                     gcCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa    <  2:2249202/101‑1 (MQ=255)
                                                                                                      ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc   <  1:1472175/101‑1 (MQ=255)
                                                                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg  >  2:3179529/1‑101 (MQ=255)
                                                                                                       cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg  >  1:5249334/1‑101 (MQ=255)
                                                                                                       |                                                                                                      
TCCAGCTCGCGCCACATGTCTTCGACCGTGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGTGTCTGCCA.CGACGCCCTG.CACCAGATCC.GCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACG  >  NC_014034/1397133‑1397333

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG