Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,276 C→G 1.8% R386R (CGC→CGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2760C→G 1.8% 4.7 ‑0.8 ‑0.5329R386R (CGC→CGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (169/154):  total (171/158)
Fisher's exact test strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.38e-01

Only 100 of 362 total aligned reads displayed.

CGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCC  >  NC_014034/1397178‑1397376
                                                                                                    |                                                                                                     
  cgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGa                                                                                                   >  1:3649745/1‑101 (MQ=255)
  cgGCGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAAC.ACCGC.GCACGCGCGa                                                                                                   >  1:5615632/1‑101 (MQ=255)
   gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGaa                                                                                                  >  1:2601232/1‑101 (MQ=255)
      ggCGTGGATCGAAACGTGCCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACgg                                                                                               <  2:1503360/101‑1 (MQ=255)
       gCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGc                                                                                              >  1:4578070/1‑101 (MQ=255)
          tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGG.GCACGCGCGACCGGC.gcc                                                                                          >  2:3309201/1‑99 (MQ=255)
          tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTc                                                                                          <  2:2314433/101‑1 (MQ=255)
           ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTcg                                                                                         >  2:64838/1‑100 (MQ=255)
           ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCa                                                                                         >  1:1450790/1‑101 (MQ=255)
            gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAg                                                                                        >  2:4839492/1‑101 (MQ=255)
            gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAg                                                                                        >  1:1692904/1‑101 (MQ=255)
            gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAg                                                                                        >  1:6330065/1‑101 (MQ=255)
                gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                    >  1:895199/1‑101 (MQ=255)
                 aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                   <  2:3820631/101‑1 (MQ=255)
                 aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                   <  1:849647/101‑1 (MQ=255)
                  aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCACGCGCGGGCGTGGATCCAAAC.ACCGC.GCACGCGCTAACGGC.CTGAGCCCcgc                                                                                  >  2:6732712/1‑101 (MQ=38)
                   aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                 <  2:5594282/101‑1 (MQ=255)
                       gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                             >  1:3417036/1‑101 (MQ=255)
                       gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                             >  1:5555084/1‑101 (MQ=255)
                       gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                             >  1:3815364/1‑101 (MQ=255)
                        tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGt                                                                            <  1:1597091/101‑1 (MQ=255)
                         cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                           >  2:4428407/1‑101 (MQ=255)
                         cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                           <  1:6189720/101‑1 (MQ=255)
                         cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                           <  1:4801212/101‑1 (MQ=255)
                         cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                           <  2:3637329/101‑1 (MQ=255)
                          tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCg                                                                          >  2:3551486/1‑101 (MQ=255)
                          tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCg                                                                          >  1:2014700/1‑101 (MQ=255)
                           gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                         >  2:3769163/1‑101 (MQ=255)
                           gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                         >  2:4180887/1‑101 (MQ=255)
                             cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                                                                       <  1:4726626/101‑1 (MQ=255)
                             cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                                                                       <  1:1805297/101‑1 (MQ=255)
                              acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                      <  2:4342200/101‑1 (MQ=255)
                               cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                     <  2:2158629/101‑1 (MQ=255)
                               cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                     >  2:6638689/1‑101 (MQ=255)
                                 acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                   >  1:5421414/1‑101 (MQ=255)
                                 acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                   <  2:5508927/101‑1 (MQ=255)
                                  cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                  >  1:1274117/1‑101 (MQ=255)
                                  cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                  <  2:5063208/101‑1 (MQ=255)
                                  cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                  <  2:6909369/101‑1 (MQ=255)
                                       tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                                                             <  1:2355434/101‑1 (MQ=255)
                                        gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCgggg                                                            >  2:770308/1‑100 (MQ=255)
                                        gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                            <  1:3483198/101‑1 (MQ=255)
                                        gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                            >  2:1792435/1‑101 (MQ=255)
                                          acgaGACCCGCTCCCTCTCCCCCACCGCGGGGGCCGGGATCGAAAC.CCCGC.GGCCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                          <  1:5281842/98‑1 (MQ=255)
                                           ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACTCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                         >  1:6991245/1‑101 (MQ=255)
                                           ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                         >  2:140128/1‑101 (MQ=255)
                                            cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAgggg                                                        >  2:4128312/1‑98 (MQ=255)
                                            cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                        <  2:2488289/101‑1 (MQ=255)
                                             aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                       <  2:4924313/101‑1 (MQ=255)
                                              gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                      <  1:6738528/101‑1 (MQ=255)
                                                 ccggggcGTCGCTCCGCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTGCCCCCGCGGGAGCGTGGATc                                                   >  1:6855213/7‑101 (MQ=38)
                                                  cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                  <  1:4181082/101‑1 (MQ=255)
                                                  cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                  <  2:3027740/101‑1 (MQ=255)
                                                   gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                 >  2:5818015/1‑101 (MQ=255)
                                                    gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  2:4288230/98‑1 (MQ=0)
                                                    ccacGTCGCTCCCCCCGCGGGGGCGTGAAGCGAAAC.AAGGCGGCGCGGGCCTATGGCTGTCGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                <  2:53700/98‑1 (MQ=2)
                                                      ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGGGTGGATCGAAAc                                              <  1:5088036/101‑1 (MQ=255)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCTAAAC.ACCGA.GCACGCGCGAACGGC.GTCAGACCCGCGAACGTCGCTCACCCCGCGGCATCGCGGATCGAAACGGc                                           >  2:4628590/1‑101 (MQ=37)
                                                         tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                           <  2:1692843/101‑1 (MQ=255)
                                                            cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                        <  2:5615342/101‑1 (MQ=255)
                                                             tCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                       >  2:6026880/1‑101 (MQ=255)
                                                              ccccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                      >  2:1234195/1‑101 (MQ=255)
                                                                ccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAg                                    <  2:839955/101‑1 (MQ=255)
                                                                 cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                   >  1:3987766/1‑101 (MQ=255)
                                                                  ccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGcc                                  <  2:4508244/101‑1 (MQ=255)
                                                                  ccGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                  <  1:4260198/101‑10 (MQ=0)
                                                                   cgcgGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                 <  1:993999/101‑11 (MQ=0)
                                                                    gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                <  1:3765959/101‑1 (MQ=255)
                                                                    gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                <  1:799123/101‑1 (MQ=255)
                                                                      gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                              <  2:2113790/101‑1 (MQ=255)
                                                                      gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                              <  2:2881055/101‑1 (MQ=255)
                                                                         ggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCgcgc                           >  1:3318676/1‑101 (MQ=255)
                                                                         ggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGACTTCgcgc                           >  1:7051687/1‑101 (MQ=255)
                                                                           cGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                         >  2:621687/1‑101 (MQ=255)
                                                                            gTGGATCGAAAC.ACCGC.GCACGCGCGAGCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                        <  2:1823939/101‑1 (MQ=255)
                                                                            gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                        <  1:4896608/101‑1 (MQ=255)
                                                                            gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                        >  1:2183826/1‑101 (MQ=255)
                                                                            gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGGGGATCGAAACGGCTTTACAGCCTTCGCGcccc                        >  1:4857641/1‑101 (MQ=255)
                                                                             tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                       <  1:6515439/101‑1 (MQ=255)
                                                                              ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      <  1:662963/101‑1 (MQ=255)
                                                                              ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      <  2:1308071/101‑1 (MQ=255)
                                                                              ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                      <  1:2983871/101‑1 (MQ=255)
                                                                               gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                     >  1:600075/1‑101 (MQ=255)
                                                                                aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                    >  1:5338180/1‑101 (MQ=255)
                                                                                aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                    <  2:3424445/101‑1 (MQ=255)
                                                                                 tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                   <  1:3086659/101‑1 (MQ=255)
                                                                                 tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                   >  2:3689343/1‑101 (MQ=255)
                                                                                  cGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                  >  1:436599/1‑101 (MQ=255)
                                                                                     aaC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac               >  2:2443463/1‑101 (MQ=255)
                                                                                          ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac           <  2:2786033/101‑1 (MQ=255)
                                                                                          ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac           <  2:4031895/101‑1 (MQ=255)
                                                                                            gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt         <  1:4428629/101‑1 (MQ=255)
                                                                                            gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt         >  2:5688286/1‑101 (MQ=255)
                                                                                             c.gcACGCGCGGACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc        >  1:2106579/1‑101 (MQ=255)
                                                                                             c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc        >  1:1212226/1‑101 (MQ=255)
                                                                                                 aCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt     <  2:2664485/101‑1 (MQ=255)
                                                                                                  cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc    <  2:6346617/101‑1 (MQ=255)
                                                                                                   gcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc   >  1:6788199/1‑101 (MQ=255)
                                                                                                    cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc  <  1:2468204/101‑1 (MQ=255)
                                                                                                    cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc  <  2:970400/101‑1 (MQ=255)
                                                                                                    |                                                                                                     
CGCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCC  >  NC_014034/1397178‑1397376

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG