Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,279 G→C 2.1% A387A (GCG→GCC RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2790G→C 2.1% 6.0 ‑1.3 ‑0.0326A387A (GCG→GCCRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (166/153):  total (168/158)
Fisher's exact test strand distribution p-value = 2.71e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.40e-01

Only 100 of 372 total aligned reads displayed.

GCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCC  >  NC_014034/1397179‑1397379
                                                                                                     |                                                                                                     
gcgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgcgcg                                                                                                       >  1:4392403/1‑101 (MQ=255)
gcgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgcgcg                                                                                                       <  2:5050199/101‑1 (MQ=255)
gcgGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCAcgcgcg                                                                                                       <  2:4407645/101‑1 (MQ=255)
 cgGCGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATAGAAACACCGC.GCACGCGCGa                                                                                                      >  1:5615632/1‑101 (MQ=255)
  gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGaa                                                                                                     >  1:2601232/1‑101 (MQ=255)
   ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAAc                                                                                                    <  1:682326/101‑1 (MQ=255)
     ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACgg                                                                                                  >  1:4212699/1‑101 (MQ=255)
      gCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGc                                                                                                 <  2:804849/101‑1 (MQ=255)
       cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                               >  2:6402296/1‑101 (MQ=255)
       cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                               >  2:4340806/1‑101 (MQ=255)
       cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCATGGATCGAAACACCGC.GCACGCGCGAACGGC.g                                                                                               >  1:4866225/1‑101 (MQ=255)
         tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTc                                                                                             >  2:3618683/1‑101 (MQ=255)
          ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCa                                                                                            >  1:1450790/1‑101 (MQ=255)
          ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCa                                                                                            <  2:6067534/101‑1 (MQ=255)
           gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAg                                                                                           >  2:4082779/1‑101 (MQ=255)
           gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAg                                                                                           >  1:6330065/1‑101 (MQ=255)
           gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAg                                                                                           >  1:1692904/1‑101 (MQ=255)
              cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                        >  2:3259580/1‑101 (MQ=255)
              cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGccc                                                                                        >  1:290781/1‑101 (MQ=255)
               gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                       <  2:2997538/101‑1 (MQ=255)
               gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                       <  2:1573380/101‑1 (MQ=255)
               gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGcccc                                                                                       >  1:895199/1‑101 (MQ=255)
                aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcg                                                                                      >  2:4576990/1‑101 (MQ=255)
                 aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgc                                                                                     >  1:3066188/1‑101 (MQ=255)
                  aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                    <  2:6766911/101‑1 (MQ=255)
                  aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                    >  1:3672515/1‑101 (MQ=255)
                  aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCcgcg                                                                                    >  1:5415301/1‑101 (MQ=255)
                   cGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGa                                                                                   >  1:831039/1‑101 (MQ=255)
                    gtgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGaa                                                                                  >  2:2396017/1‑101 (MQ=255)
                     tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                 <  2:3450733/101‑1 (MQ=255)
                     tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                 <  1:3779291/101‑1 (MQ=255)
                     tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAAc                                                                                 >  1:3961075/1‑101 (MQ=255)
                      gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACg                                                                                >  2:2544887/1‑101 (MQ=255)
                      gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCCGGGGCGGGGGTCGGGACAACGC.GCGCGGGCGAACGGA.GGCAGcccgccgccgg                                                                                >  2:392584/1‑93 (MQ=255)
                        cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                              <  1:6189720/101‑1 (MQ=255)
                         tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCg                                                                             >  1:2014700/1‑101 (MQ=255)
                           gcccgacgACCCGCACCAGATCCGCCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCt                                                                           <  2:2265545/98‑1 (MQ=38)
                            cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                                                                          >  2:3379763/1‑101 (MQ=255)
                             acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                         >  2:53642/1‑101 (MQ=255)
                              cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                        <  2:2158629/101‑1 (MQ=255)
                               gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                                                                       >  2:6515109/1‑101 (MQ=255)
                               gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccc                                                                       <  1:1620841/101‑1 (MQ=255)
                                acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                      <  2:5508927/101‑1 (MQ=255)
                                acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                      <  1:5503543/101‑1 (MQ=255)
                                 cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                     >  1:1274117/1‑101 (MQ=255)
                                      tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                                                                <  1:2355434/101‑1 (MQ=255)
                                       gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCgggg                                                               >  2:5827817/1‑100 (MQ=255)
                                       gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                               >  2:1792435/1‑101 (MQ=255)
                                          ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                            >  2:140128/1‑101 (MQ=255)
                                            aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                          <  2:4924313/101‑1 (MQ=255)
                                                 cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                     >  1:4646763/1‑101 (MQ=255)
                                                     gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAAAA.GCCGCCGACAAACGC.GGCAGCCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                 <  2:3743876/99‑1 (MQ=2)
                                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                >  1:5249334/1‑101 (MQ=255)
                                                      cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                <  2:2747782/101‑1 (MQ=255)
                                                       gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                               >  2:718577/1‑101 (MQ=255)
                                                        tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                              <  2:1692843/101‑1 (MQ=255)
                                                          gCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaagaggtt                                            >  1:3337490/1‑95 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACCCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                           <  1:3309317/101‑1 (MQ=255)
                                                           cTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                           <  2:5615342/101‑1 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                          <  1:5874045/101‑1 (MQ=255)
                                                            tCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                          <  1:6670419/101‑1 (MQ=255)
                                                            cccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                          <  2:4200875/100‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                         <  1:64839/101‑1 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                         >  2:1234195/1‑101 (MQ=255)
                                                             ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                         <  2:4710784/101‑1 (MQ=255)
                                                              cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                        <  1:4090185/101‑1 (MQ=255)
                                                                cccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                      <  2:6651711/101‑1 (MQ=255)
                                                                  cgcgGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                    <  1:993999/101‑11 (MQ=0)
                                                                   gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAGGGCTTTACAGCCtt                                   >  2:3662862/1‑101 (MQ=255)
                                                                   gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                   >  1:6977224/1‑101 (MQ=255)
                                                                   gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                   <  1:799123/101‑1 (MQ=255)
                                                                   gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                   <  1:430923/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                 <  2:2113790/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                 >  2:4172164/1‑101 (MQ=255)
                                                                     gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                 <  1:1723515/101‑1 (MQ=255)
                                                                     gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                 <  2:2881055/101‑1 (MQ=255)
                                                                      ggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTCTACAGCCTTcgc                                <  1:5054991/101‑1 (MQ=255)
                                                                       gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                               <  2:5579068/101‑1 (MQ=255)
                                                                       gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGGGGATCGAAACGGCTTTACAGCCTTcgcg                               >  1:6777058/1‑101 (MQ=255)
                                                                        ggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGACTTCgcgc                              >  1:7051687/1‑101 (MQ=255)
                                                                           gTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                           >  2:3800255/1‑101 (MQ=255)
                                                                           gTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                           >  1:2183826/1‑101 (MQ=255)
                                                                             ggATCGAAACCCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         <  2:4459897/101‑1 (MQ=255)
                                                                             ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         <  2:1308071/101‑1 (MQ=255)
                                                                             ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                         >  1:4573352/1‑101 (MQ=255)
                                                                               aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                       >  1:5338180/1‑101 (MQ=255)
                                                                               aTCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACATCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                       >  2:2470023/1‑101 (MQ=255)
                                                                                 cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                     >  1:3468155/1‑101 (MQ=255)
                                                                                   aaaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAga                   <  2:6282984/101‑1 (MQ=255)
                                                                                    aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                  >  2:5752643/1‑101 (MQ=255)
                                                                                       acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga               >  1:6605257/1‑101 (MQ=255)
                                                                                        ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac              <  1:4839735/101‑1 (MQ=255)
                                                                                         cgc.gCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg             <  2:1832656/101‑1 (MQ=255)
                                                                                           c.gcACGCGCGGACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc           >  1:2106579/1‑101 (MQ=255)
                                                                                           c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc           >  1:1212226/1‑101 (MQ=255)
                                                                                                 gcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc      >  1:4738896/1‑101 (MQ=255)
                                                                                                  cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc     <  1:2468204/101‑1 (MQ=255)
                                                                                                  cgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc     <  2:970400/101‑1 (MQ=255)
                                                                                                   gcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc    <  1:3618832/101‑1 (MQ=255)
                                                                                                    cgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc   <  2:854221/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
GCGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCC  >  NC_014034/1397179‑1397379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG