Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,280 A→T 1.8% N388Y (AAC→TAT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2800A→T 1.8% 7.6 ‑0.4 ‑0.7338N388Y (AAC→TAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (165/166):  total (168/170)
Fisher's exact test strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.19e-01

Only 100 of 369 total aligned reads displayed.

CGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCG  >  NC_014034/1397180‑1397380
                                                                                                      |                                                                                                       
 gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGaa                                                                                                        >  2:2151813/1‑101 (MQ=255)
 gggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGaa                                                                                                        >  1:2601232/1‑101 (MQ=255)
  ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAAc                                                                                                       >  2:3765798/1‑101 (MQ=255)
  ggggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAAc                                                                                                       <  1:4261208/101‑1 (MQ=255)
    ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACgg                                                                                                     <  2:2967750/101‑1 (MQ=255)
    ggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACgg                                                                                                     >  1:4212699/1‑101 (MQ=255)
     gCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGc                                                                                                    <  2:804849/101‑1 (MQ=255)
      cGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCATGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.g                                                                                                  >  1:4866225/1‑101 (MQ=255)
       gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.Gt                                                                                                 >  1:532027/1‑101 (MQ=255)
       gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.Gt                                                                                                 <  1:2067401/101‑1 (MQ=255)
        tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTc                                                                                                >  2:1266700/1‑101 (MQ=255)
        tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTc                                                                                                >  2:3354071/1‑101 (MQ=255)
        tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCACATCCGCGCCGACGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTc                                                                                                >  1:697312/1‑101 (MQ=255)
         ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.a                                                                                              <  2:6067534/101‑1 (MQ=255)
         ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.a                                                                                              >  2:2355339/1‑101 (MQ=255)
         ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.a                                                                                              >  1:1450790/1‑101 (MQ=255)
          gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                             >  1:1692904/1‑101 (MQ=255)
            tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                                                                          >  2:1982035/1‑101 (MQ=255)
             cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                         >  1:290781/1‑101 (MQ=255)
              gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                        >  1:4583893/1‑101 (MQ=255)
               aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                       <  2:3820631/101‑1 (MQ=255)
               aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                       >  1:3896446/1‑101 (MQ=255)
                aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgc                                                                                      >  1:7039958/1‑101 (MQ=255)
                aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgc                                                                                      >  2:4908497/1‑101 (MQ=255)
                 aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                     >  1:3672515/1‑101 (MQ=255)
                 aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                     >  1:5403798/1‑101 (MQ=255)
                    tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                                                                  <  1:3779291/101‑1 (MQ=255)
                    tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                                                                  >  1:6248451/1‑101 (MQ=255)
                       cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                               <  2:3637329/101‑1 (MQ=255)
                       cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                               >  1:949375/1‑101 (MQ=255)
                        tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCg                                                                              >  1:2014700/1‑101 (MQ=255)
                         gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGc                                                                             >  2:4180887/1‑101 (MQ=255)
                           cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTc                                                                           >  1:6345307/1‑101 (MQ=255)
                            acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcc                                                                          <  2:4342200/101‑1 (MQ=255)
                             cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccc                                                                         <  1:4838715/101‑1 (MQ=255)
                             cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccc                                                                         >  2:6638689/1‑101 (MQ=255)
                             cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccc                                                                         >  2:2460648/1‑101 (MQ=255)
                              gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                                                                        >  2:6515109/1‑101 (MQ=255)
                              gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                                                                        <  1:1620841/101‑1 (MQ=255)
                              gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                                                                        <  1:6635970/101‑1 (MQ=255)
                               gcgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                       <  2:4451679/100‑1 (MQ=255)
                                cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                      >  1:1274117/1‑101 (MQ=255)
                                     tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                 <  1:2355434/101‑1 (MQ=255)
                                      gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGa                                                                <  1:864988/101‑1 (MQ=255)
                                       cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAg                                                               <  2:547647/101‑1 (MQ=255)
                                       cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAg                                                               >  1:3251026/1‑101 (MQ=255)
                                        aCCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                              >  2:167990/1‑101 (MQ=255)
                                         ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                             >  1:361726/1‑101 (MQ=255)
                                          cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAgggg                                                            >  2:4128312/1‑98 (MQ=255)
                                           aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                           <  2:1447720/101‑1 (MQ=255)
                                            gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                          <  1:6738528/101‑1 (MQ=255)
                                            gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                          <  1:3012913/101‑1 (MQ=255)
                                                 gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                     >  2:5818015/1‑101 (MQ=255)
                                                  gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  2:4288230/98‑1 (MQ=0)
                                                  cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                    <  2:2722899/101‑1 (MQ=255)
                                                  cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                    >  2:135323/1‑101 (MQ=255)
                                                  cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                    <  1:5132623/101‑1 (MQ=255)
                                                   gcCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                   <  2:2249202/101‑1 (MQ=255)
                                                     cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                 <  1:68039/101‑1 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                >  1:71308/1‑101 (MQ=255)
                                                      gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                >  1:4956331/1‑101 (MQ=255)
                                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                              >  1:5498335/1‑101 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaagaggtt                                             >  1:3337490/1‑95 (MQ=255)
                                                         gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                             >  1:5873433/1‑101 (MQ=255)
                                                          cgccccccGCGGGGGCGTCGATCGAAAG.TGCGT.GCGCGCCATGAATGC.ATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacca                                              <  2:4963092/99‑5 (MQ=0)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGccat                                            >  2:1720686/1‑98 (MQ=255)
                                                          cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                            <  2:5615342/101‑1 (MQ=255)
                                                                 cgcgGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                     >  1:4387133/1‑91 (MQ=0)
                                                                  gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                    <  2:181490/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                   <  2:4629331/101‑1 (MQ=255)
                                                                   cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                   <  1:999544/101‑1 (MQ=255)
                                                                    gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                  >  2:5226673/1‑101 (MQ=255)
                                                                    gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                  <  1:1723515/101‑1 (MQ=255)
                                                                     ggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgc                                 >  2:3682155/1‑101 (MQ=255)
                                                                      gggGGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGATACGGCTTTACAGCCTTcgcg                                >  1:5614152/1‑101 (MQ=255)
                                                                      gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                >  1:6185640/1‑101 (MQ=255)
                                                                      gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                <  2:5579068/101‑1 (MQ=255)
                                                                         cGTGGGTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                             >  2:3518890/1‑101 (MQ=255)
                                                                         cGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                             >  2:621687/1‑101 (MQ=255)
                                                                          gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                            >  2:6959754/1‑101 (MQ=255)
                                                                          gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                            >  1:2183826/1‑101 (MQ=255)
                                                                           tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                           >  2:6743076/1‑101 (MQ=255)
                                                                           tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                           <  1:6515439/101‑1 (MQ=255)
                                                                            ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                          >  1:821802/1‑101 (MQ=255)
                                                                             gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                         >  2:4557352/1‑101 (MQ=255)
                                                                                cGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                      >  1:3468155/1‑101 (MQ=255)
                                                                                 gAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAg                     <  1:4009363/101‑1 (MQ=255)
                                                                                  aaaC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAga                    >  2:2528051/1‑101 (MQ=255)
                                                                                     c.acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                 >  1:4727701/1‑101 (MQ=255)
                                                                                     c.acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                 >  2:2801286/1‑101 (MQ=255)
                                                                                        ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac               <  2:4031895/101‑1 (MQ=255)
                                                                                          gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt             >  2:5688286/1‑101 (MQ=255)
                                                                                           c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc            <  1:2460742/101‑1 (MQ=255)
                                                                                           c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc            >  1:1212226/1‑101 (MQ=255)
                                                                                                cgcgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc        <  2:6346617/101‑1 (MQ=255)
                                                                                                  cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc      <  2:970400/101‑1 (MQ=255)
                                                                                                    cgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc    <  2:854221/101‑1 (MQ=255)
                                                                                                     gAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGTCCCACGCCAGACGGACGTCGCTcccccc   <  2:3380833/101‑1 (MQ=255)
                                                                                                     gAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc   >  2:3098238/1‑101 (MQ=255)
                                                                                                      aaCGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg  >  1:4558727/1‑101 (MQ=255)
                                                                                                      |                                                                                                       
CGGGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCG  >  NC_014034/1397180‑1397380

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG