Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,282 C→T 1.8% N388N (AAC→TAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2820C→T 1.8% 6.8 ‑0.8 ‑0.1336N388N (AAC→AATRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (166/163):  total (168/168)
Fisher's exact test strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.39e-01

Only 100 of 363 total aligned reads displayed.

GGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCG  >  NC_014034/1397182‑1397380
                                                                                                      |                                                                                                     
 gggCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACg                                                                                                      >  1:4249402/1‑101 (MQ=255)
  ggCGTGGATCGAAACGTGCCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACgg                                                                                                     <  2:1503360/101‑1 (MQ=255)
     gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.Gt                                                                                                 <  1:2067401/101‑1 (MQ=255)
       ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTcg                                                                                               >  2:64838/1‑100 (MQ=255)
       ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.a                                                                                              >  2:3359939/1‑101 (MQ=255)
       ggATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.a                                                                                              >  1:1450790/1‑101 (MQ=255)
        gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                             >  1:1692904/1‑101 (MQ=255)
        gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                             <  2:4134121/101‑1 (MQ=255)
        gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                             >  2:1389352/1‑101 (MQ=255)
        gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                             >  2:4636967/1‑101 (MQ=255)
          tCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.c                                                                                          >  2:1982035/1‑101 (MQ=255)
            gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                        >  1:895199/1‑101 (MQ=255)
             aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                       <  2:3820631/101‑1 (MQ=255)
             aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                       <  2:4342411/101‑1 (MQ=255)
              aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgc                                                                                      >  2:4908497/1‑101 (MQ=255)
               aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                     >  1:5415301/1‑101 (MQ=255)
                cGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGa                                                                                    >  1:831039/1‑101 (MQ=255)
                   gtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACg                                                                                 >  2:2544887/1‑101 (MQ=255)
                    tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGt                                                                                <  1:1597091/101‑1 (MQ=255)
                     cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                               <  1:4801212/101‑1 (MQ=255)
                      tGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCg                                                                              >  2:3551486/1‑101 (MQ=255)
                       gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGc                                                                             >  2:4180887/1‑101 (MQ=255)
                       gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGc                                                                             >  2:3769163/1‑101 (MQ=255)
                        gcccgacgACCCGCACCAGATCCGCCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCt                                                                            <  2:2265545/98‑1 (MQ=38)
                          ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcc                                                                          <  2:2745230/100‑1 (MQ=255)
                           cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccc                                                                         >  2:6638689/1‑101 (MQ=255)
                           cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccc                                                                         <  2:2158629/101‑1 (MQ=255)
                             gcgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                       <  2:4451679/100‑1 (MQ=255)
                             acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                       >  1:1980130/1‑101 (MQ=255)
                             acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                       <  1:5503543/101‑1 (MQ=255)
                              cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                      >  1:1274117/1‑101 (MQ=255)
                               gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccccc                                                                     >  1:5707122/1‑100 (MQ=255)
                               gCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCcg                                                                     <  2:1756289/101‑1 (MQ=255)
                                   tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                 <  1:2355434/101‑1 (MQ=255)
                                    gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGa                                                                <  1:3483198/101‑1 (MQ=255)
                                     cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAg                                                               <  2:547647/101‑1 (MQ=255)
                                           aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa                                                         >  1:413206/1‑101 (MQ=255)
                                             ccggggcGTCGCTCCGCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTGCCCCCGCGGGAGCGTGGATc                                                       >  1:6855213/7‑101 (MQ=38)
                                             ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                       <  1:468695/101‑1 (MQ=255)
                                             ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                       <  1:6222569/101‑1 (MQ=255)
                                               gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                     >  2:5818015/1‑101 (MQ=255)
                                                gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                    <  2:4288230/98‑1 (MQ=0)
                                                cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                    <  1:3360062/101‑1 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                 >  2:3179529/1‑101 (MQ=255)
                                                   cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                 >  1:5249334/1‑101 (MQ=255)
                                                    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                >  1:3779300/1‑101 (MQ=255)
                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                               <  2:1692843/101‑1 (MQ=255)
                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                              <  1:4477994/101‑1 (MQ=255)
                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                             <  1:6437240/101‑1 (MQ=255)
                                                        cgccccccGCGGGGGCGTCGATCGAAAG.TGCGT.GCGCGCCATGAATGC.ATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacca                                              <  2:4963092/99‑5 (MQ=0)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                            <  1:3309317/101‑1 (MQ=255)
                                                        cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                            >  2:2865945/1‑101 (MQ=255)
                                                         tCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                           >  2:6026880/1‑101 (MQ=255)
                                                           cccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                         >  2:120715/1‑101 (MQ=255)
                                                              ccGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgggcc                                      >  2:2363584/1‑92 (MQ=0)
                                                                gcgGGGGCGTGGCTCGAAAC.ACCGC.GCAGGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCTCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                    >  1:6283307/1‑101 (MQ=255)
                                                                gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                    >  2:811360/1‑101 (MQ=255)
                                                                gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                    >  1:6977224/1‑101 (MQ=255)
                                                                  gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                  <  1:1723515/101‑1 (MQ=255)
                                                                  gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                  >  2:5226673/1‑101 (MQ=255)
                                                                   ggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTCTACAGCCTTcgc                                 <  1:5054991/101‑1 (MQ=255)
                                                                    gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                <  2:5579068/101‑1 (MQ=255)
                                                                    gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                >  1:970434/1‑101 (MQ=255)
                                                                    gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGGGGATCGAAACGGCTTTACAGCCTTcgcg                                >  1:6777058/1‑101 (MQ=255)
                                                                     ggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGACTTCgcgc                               >  1:7051687/1‑101 (MQ=255)
                                                                       cGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                             >  2:621687/1‑101 (MQ=255)
                                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAGCGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                            <  2:1823939/101‑1 (MQ=255)
                                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                            >  1:2183826/1‑101 (MQ=255)
                                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                            >  2:6959754/1‑101 (MQ=255)
                                                                        gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                            <  1:4896608/101‑1 (MQ=255)
                                                                         tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                           <  2:3649596/101‑1 (MQ=255)
                                                                         tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                           >  2:6743076/1‑101 (MQ=255)
                                                                         tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                           <  1:3971088/101‑1 (MQ=255)
                                                                          ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                          <  2:1308071/101‑1 (MQ=255)
                                                                          ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                          >  1:4573352/1‑101 (MQ=255)
                                                                          ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                          <  1:2983871/101‑1 (MQ=255)
                                                                           gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                         <  1:786515/101‑1 (MQ=255)
                                                                           gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                         >  2:4557352/1‑101 (MQ=255)
                                                                           gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                         >  1:600075/1‑101 (MQ=255)
                                                                            aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                        <  2:3424445/101‑1 (MQ=255)
                                                                            aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                        >  1:5338180/1‑101 (MQ=255)
                                                                            aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACATCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                        >  2:2470023/1‑101 (MQ=255)
                                                                             tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                       <  1:3086659/101‑1 (MQ=255)
                                                                             tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                       >  2:3689343/1‑101 (MQ=255)
                                                                                aaaC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAga                    <  2:5609078/101‑1 (MQ=255)
                                                                                     acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                >  1:6605257/1‑101 (MQ=255)
                                                                                      ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac               <  1:3757923/101‑1 (MQ=255)
                                                                                      ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac               <  2:4031895/101‑1 (MQ=255)
                                                                                      ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac               <  1:4839735/101‑1 (MQ=255)
                                                                                        gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt             >  2:5688286/1‑101 (MQ=255)
                                                                                         c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc            >  1:1212226/1‑101 (MQ=255)
                                                                                            cACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc          <  1:4341028/101‑1 (MQ=255)
                                                                                              cgcgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc        <  1:663638/101‑1 (MQ=255)
                                                                                              cgcgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc        <  2:6329740/101‑1 (MQ=255)
                                                                                               gcgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc       <  2:2981584/101‑1 (MQ=255)
                                                                                                cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc      <  1:2468204/101‑1 (MQ=255)
                                                                                                cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc      <  2:970400/101‑1 (MQ=255)
                                                                                                 gcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc     <  1:3618832/101‑1 (MQ=255)
                                                                                                  cgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc    <  1:2618741/101‑1 (MQ=255)
                                                                                                  cgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccccc    <  2:854221/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
GGGGCGTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCG  >  NC_014034/1397182‑1397380

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG