Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,287 T→A 1.9% V390D (GTC→GAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2870T→A 1.9% 7.3 ‑0.4 ‑0.1322V390D (GTC→GAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/4):  ref base (158/157):  total (160/162)
Fisher's exact test strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.82e-01

Only 100 of 349 total aligned reads displayed.

GTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397187‑1397387
                                                                                                       |                                                                                                      
gTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.Gt                                                                                                        <  1:2067401/101‑1 (MQ=255)
 tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTc                                                                                                       >  2:3354071/1‑101 (MQ=255)
 tGGATCGAAACGTGTCTGCCACGACGCCCTGCACCACATCCGCGCCGACGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTc                                                                                                       >  1:697312/1‑101 (MQ=255)
   gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                                    <  2:4134121/101‑1 (MQ=255)
   gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                                    >  2:4636967/1‑101 (MQ=255)
   gATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.Ag                                                                                                    >  1:1692904/1‑101 (MQ=255)
      cGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.cc                                                                                                <  1:3445800/101‑1 (MQ=255)
       gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                               <  2:2997538/101‑1 (MQ=255)
       gAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGc.ccc                                                                                               >  1:854245/1‑101 (MQ=255)
        aaaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcg                                                                                              <  2:4342411/101‑1 (MQ=255)
         aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgc                                                                                             >  1:7039958/1‑101 (MQ=255)
         aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgc                                                                                             >  2:4908497/1‑101 (MQ=255)
         aaCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgc                                                                                             >  1:3066188/1‑101 (MQ=255)
          aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                            >  1:5415301/1‑101 (MQ=255)
          aCGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCcgcg                                                                                            >  1:3672515/1‑101 (MQ=255)
             tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                                                                         >  1:6248451/1‑101 (MQ=255)
             tgtCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAAc                                                                                         <  1:3779291/101‑1 (MQ=255)
               tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGt                                                                                       <  1:1597091/101‑1 (MQ=255)
                cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                                      <  1:4801212/101‑1 (MQ=255)
                cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                                      <  2:3637329/101‑1 (MQ=255)
                cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                                      <  1:6189720/101‑1 (MQ=255)
                cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTc                                                                                      >  1:949375/1‑101 (MQ=255)
                  gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGc                                                                                    >  2:3769163/1‑101 (MQ=255)
                  gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGc                                                                                    >  2:4180887/1‑101 (MQ=255)
                   gcccgacgACCCGCACCAGATCCGCCCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCt                                                                                   <  2:2265545/98‑1 (MQ=38)
                     ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcc                                                                                 <  2:2745230/100‑1 (MQ=255)
                     acgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcc                                                                                 >  2:53642/1‑101 (MQ=255)
                       gacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccc                                                                               <  1:6635970/101‑1 (MQ=255)
                        acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                              >  1:1980130/1‑101 (MQ=255)
                        acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTccccc                                                                              <  2:5508927/101‑1 (MQ=255)
                         cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                             <  2:2538734/101‑1 (MQ=255)
                         cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                             >  1:1274117/1‑101 (MQ=255)
                         cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTcccccc                                                                             <  2:6909369/101‑1 (MQ=255)
                              tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCggg                                                                        <  1:2355434/101‑1 (MQ=255)
                               gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCgggg                                                                       >  2:770308/1‑100 (MQ=255)
                                cACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAg                                                                      >  1:3251026/1‑101 (MQ=255)
                                 acgaGACCCGCTCCCTCTCCCCCACCGCGGGGGCCGGGATCGAAAC.CCCGC.GGCCGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                     <  1:5281842/98‑1 (MQ=255)
                                  ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                    >  1:361726/1‑101 (MQ=255)
                                  ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                    <  2:1718635/101‑1 (MQ=255)
                                     gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                 <  1:6738528/101‑1 (MQ=255)
                                      aTCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGa                                                                >  1:413206/1‑101 (MQ=255)
                                        ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                              <  2:6525563/101‑1 (MQ=255)
                                        ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                              <  1:468695/101‑1 (MQ=255)
                                          gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                            >  2:5818015/1‑101 (MQ=255)
                                           gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  2:4288230/98‑1 (MQ=0)
                                           cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                           >  2:135323/1‑101 (MQ=255)
                                             ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGGGTGGATCGAAAc                                                         <  1:5088036/101‑1 (MQ=255)
                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                        <  1:68039/101‑1 (MQ=255)
                                              cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                        <  2:3867474/101‑1 (MQ=255)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                       >  1:71308/1‑101 (MQ=255)
                                               gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                       >  1:140021/1‑101 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                     >  1:5498335/1‑101 (MQ=255)
                                                 cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                     >  2:1427032/1‑101 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaagaggtt                                                    >  1:3337490/1‑95 (MQ=255)
                                                  gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                    >  1:2664581/1‑101 (MQ=255)
                                                   cgccccccGCGGGGGCGTCGATCGAAAG.TGCGT.GCGCGCCATGAATGC.ATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacca                                                     <  2:4963092/99‑5 (MQ=0)
                                                   cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                   <  2:5615342/101‑1 (MQ=255)
                                                    tCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTa                                                  <  1:5874045/101‑1 (MQ=255)
                                                     ccccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTAc                                                 >  2:1234195/1‑101 (MQ=255)
                                                       ccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCCCCGGAGCCGGGATCGAAACGGCTTTACAg                                               >  2:86111/1‑101 (MQ=39)
                                                        cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                              >  1:3987766/1‑101 (MQ=255)
                                                        cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                              <  2:6651711/101‑1 (MQ=255)
                                                         ccGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgggcc                                             >  2:2363584/1‑92 (MQ=0)
                                                          cgcgGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGAC.GGA.CCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcca                                            <  1:996842/101‑11 (MQ=0)
                                                            cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                          <  1:2151893/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                         <  2:2113790/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                         <  2:5961788/101‑1 (MQ=255)
                                                             gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                         <  1:1723515/101‑1 (MQ=255)
                                                               gggGGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGATACGGCTTTACAGCCTTcgcg                                       >  1:5614152/1‑101 (MQ=255)
                                                               gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                       <  2:5579068/101‑1 (MQ=255)
                                                                  cGTGGGTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                    >  2:3518890/1‑101 (MQ=255)
                                                                  cGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                    <  1:4908741/101‑1 (MQ=255)
                                                                  cGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                    >  2:621687/1‑101 (MQ=255)
                                                                    tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                  <  1:6515439/101‑1 (MQ=255)
                                                                     ggATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 <  2:4459897/101‑1 (MQ=255)
                                                                     ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 >  1:821802/1‑101 (MQ=255)
                                                                     ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 <  1:2983871/101‑1 (MQ=255)
                                                                     ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                 >  1:4573352/1‑101 (MQ=255)
                                                                      gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                >  2:4557352/1‑101 (MQ=255)
                                                                       aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                               >  1:5338180/1‑101 (MQ=255)
                                                                       aTCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACATCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGc                               >  2:2470023/1‑101 (MQ=255)
                                                                        tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                              <  2:1804385/101‑1 (MQ=255)
                                                                        tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                              >  2:3689343/1‑101 (MQ=255)
                                                                              c.acCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                        >  2:2801286/1‑101 (MQ=255)
                                                                                 ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                      <  1:4839735/101‑1 (MQ=255)
                                                                                 ccGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggac                      <  2:4031895/101‑1 (MQ=255)
                                                                                  cgc.gCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacggacg                     <  2:1832656/101‑1 (MQ=255)
                                                                                   gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                    <  1:4428629/101‑1 (MQ=255)
                                                                                   gc.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                    >  2:5688286/1‑101 (MQ=255)
                                                                                    c.gcACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                   >  1:1212226/1‑101 (MQ=255)
                                                                                       cACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc                 <  1:4341028/101‑1 (MQ=255)
                                                                                         cgcgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc               <  2:6346617/101‑1 (MQ=255)
                                                                                           cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc             <  1:2468204/101‑1 (MQ=255)
                                                                                           cgcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTccc             <  2:970400/101‑1 (MQ=255)
                                                                                            gcgAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc            <  1:6420389/101‑1 (MQ=255)
                                                                                              gAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGTCCCACGCCAGACGGACGTCGCTcccccc          <  2:3380833/101‑1 (MQ=255)
                                                                                              gAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc          >  2:3098238/1‑101 (MQ=255)
                                                                                                  ggC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgg      >  2:2189054/1‑101 (MQ=255)
                                                                                                      gTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg   >  1:3837082/1‑101 (MQ=255)
                                                                                                       tC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc  >  2:1617121/1‑101 (MQ=255)
                                                                                                       |                                                                                                      
GTGGATCGAAACGTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTC.AGC.CCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGC  >  NC_014034/1397187‑1397387

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG