Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,299 A→C 2.2% N394T (AAC→ACC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,2990A→C 2.2% 5.2 ‑0.8 ‑0.2302N394T (AAC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/5):  ref base (136/158):  total (138/164)
Fisher's exact test strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.77e-01

Only 100 of 330 total aligned reads displayed.

GTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397199‑1397399
                                                                                                       |                                                                                                    
   tCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGt                                                                                                   >  1:6775043/1‑101 (MQ=255)
    cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                                                  >  1:949375/1‑101 (MQ=255)
    cTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTc                                                                                                  <  1:6189720/101‑1 (MQ=255)
      gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                                                >  2:4180887/1‑101 (MQ=255)
      gCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGc                                                                                                <  1:3743741/101‑1 (MQ=255)
        cACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTc                                                                                              <  1:1805297/101‑1 (MQ=255)
         ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                             <  2:4938727/100‑1 (MQ=255)
         ccgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcc                                                                                             <  2:2745230/100‑1 (MQ=255)
          cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                            >  2:2460648/1‑101 (MQ=255)
          cgacgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccc                                                                                            <  1:4838715/101‑1 (MQ=255)
            acgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTccccc                                                                                          <  2:5508927/101‑1 (MQ=255)
             cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                         >  1:1274117/1‑101 (MQ=255)
             cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                         <  2:6909369/101‑1 (MQ=255)
             cgCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTcccccc                                                                                         <  2:5063208/101‑1 (MQ=255)
                  tGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCggg                                                                                    <  1:2355434/101‑1 (MQ=255)
                     acgaGACCCGCTCCCTCTCCCCCACCGCGGGGGCCGGGATCGAAAC.CCCGC.GGCCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGc                                                                                 <  1:5281842/98‑1 (MQ=255)
                      ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACTCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                                >  1:6991245/1‑101 (MQ=255)
                      ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                                >  1:361726/1‑101 (MQ=255)
                      ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                                >  2:140128/1‑101 (MQ=255)
                       cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAgggg                                                                               >  2:4128312/1‑98 (MQ=255)
                       cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                               <  1:2548571/101‑1 (MQ=255)
                        ggccctcgcacGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTg                                                                              <  1:2986603/91‑1 (MQ=2)
                        aGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTg                                                                              <  2:1447720/101‑1 (MQ=255)
                            ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                          <  2:6525563/101‑1 (MQ=255)
                             cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                         <  2:3027740/101‑1 (MQ=255)
                             cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                         <  1:4181082/101‑1 (MQ=255)
                             cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                         >  1:4646763/1‑101 (MQ=255)
                              gcgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGa                                                                        >  2:5818015/1‑101 (MQ=255)
                               gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGG.GCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       <  2:4288230/98‑1 (MQ=0)
                               ccacGTCGCTCCCCCCGCGGGGGCGTGAAGCGAAAC.AAGGCGGCGCGGGCCTATGGCTGTCGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       <  2:53700/98‑1 (MQ=2)
                                gcCTTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaa                                                                      <  2:2249202/101‑1 (MQ=255)
                                 gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.AAAAA.GCCGCCGACAAACGC.GGCAGCCGGCCCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                     <  2:3743876/99‑1 (MQ=2)
                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                    <  2:3867474/101‑1 (MQ=255)
                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                    >  2:3179529/1‑101 (MQ=255)
                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                    <  1:68039/101‑1 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                   >  1:140021/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCTAAAC.ACCGA.GCACGCGCGAACGGC.GTCAGACCCGCGAACGTCGCTCACCCCGCGGCATCGCGGATCGAAACGGc                                                                  >  2:4628590/1‑101 (MQ=37)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                  >  2:4353285/1‑101 (MQ=255)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                  <  2:1692843/101‑1 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                 >  1:5498335/1‑101 (MQ=255)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                 >  2:3504617/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaagaggtt                                                                >  1:3337490/1‑95 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                <  1:6437240/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                <  1:6015275/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                >  1:5873433/1‑101 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                >  1:2664581/1‑101 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                               <  1:3309317/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGccat                                                               >  2:1720686/1‑98 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCACCCGCGGGAGCGTGGATCGAAACGGCttt                                                               >  1:4508483/1‑101 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                                            <  1:4090185/101‑1 (MQ=255)
                                          cccccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACa                                                            <  2:895175/101‑1 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                                          >  1:3987766/1‑101 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                                          <  2:6651711/101‑1 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGc                                                          <  1:167993/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAAC.AATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgggcc                                                         >  2:2363584/1‑92 (MQ=0)
                                              cgcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCt                                                        <  1:2023617/101‑1 (MQ=255)
                                               gcgGGGGCGTGGCTCGAAAC.ACCGC.GCAGGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCTCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                       >  1:6283307/1‑101 (MQ=255)
                                               gcgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCtt                                                       >  2:811360/1‑101 (MQ=255)
                                                cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                      <  1:2151893/101‑1 (MQ=255)
                                                cgGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTc                                                      <  1:999544/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcg                                                     <  2:5961788/101‑1 (MQ=255)
                                                  ggggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTCTACAGCCTTcgc                                                    <  1:5054991/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTcgcg                                                   <  2:5579068/101‑1 (MQ=255)
                                                      cGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGccc                                                >  1:5609368/1‑101 (MQ=255)
                                                       gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                               <  2:2014620/101‑1 (MQ=255)
                                                       gTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGcccc                                               <  1:4896608/101‑1 (MQ=255)
                                                        tGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCa                                              <  2:3649596/101‑1 (MQ=255)
                                                         ggATCGAAAC.CCCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             <  2:4459897/101‑1 (MQ=255)
                                                         ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             >  1:821802/1‑101 (MQ=255)
                                                         ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             >  2:5630909/1‑101 (MQ=255)
                                                         ggATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCAc                                             >  2:1265689/1‑101 (MQ=255)
                                                          gATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACg                                            <  1:786515/101‑1 (MQ=255)
                                                            tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                          <  1:3086659/101‑1 (MQ=255)
                                                            tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                          >  2:3689343/1‑101 (MQ=255)
                                                            tCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGcc                                          <  2:1804385/101‑1 (MQ=255)
                                                             cGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCa                                         >  1:3468155/1‑101 (MQ=255)
                                                                aaC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgac                                      >  2:5752643/1‑101 (MQ=255)
                                                                  c.acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                                    >  2:2801286/1‑101 (MQ=255)
                                                                  c.acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgg                                    >  1:4727701/1‑101 (MQ=255)
                                                                    acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAgacgga                                   >  1:6605257/1‑101 (MQ=255)
                                                                       gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGt                                <  1:4428629/101‑1 (MQ=255)
                                                                        c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTc                               >  1:1212226/1‑101 (MQ=255)
                                                                           cACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc                             <  1:4341028/101‑1 (MQ=255)
                                                                            aCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                            <  2:2664485/101‑1 (MQ=255)
                                                                             cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                           <  2:6329740/101‑1 (MQ=255)
                                                                             cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                           <  1:663638/101‑1 (MQ=255)
                                                                                gcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc                        >  1:1719235/1‑101 (MQ=255)
                                                                                  gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGTCCCACGCCAGACGGACGTCGCTcccccc                      <  2:3380833/101‑1 (MQ=255)
                                                                                        c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                >  1:5404914/1‑101 (MQ=255)
                                                                                          gTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCggggg               >  1:3837082/1‑101 (MQ=255)
                                                                                           tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc              <  2:2183731/101‑1 (MQ=255)
                                                                                            cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg             >  1:5123119/1‑101 (MQ=255)
                                                                                             aGCCCCGCGAACGTCGCGCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt            <  2:71307/101‑1 (MQ=255)
                                                                                              gCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTg           >  2:1860604/1‑101 (MQ=255)
                                                                                                  cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc       <  2:856176/101‑1 (MQ=255)
                                                                                                  cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc       <  2:2518207/101‑1 (MQ=255)
                                                                                                   gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg      <  2:4865981/101‑1 (MQ=255)
                                                                                                     gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa    <  1:3769324/101‑1 (MQ=255)
                                                                                                       aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  2:6185321/101‑1 (MQ=255)
                                                                                                       aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc  <  1:1555242/101‑1 (MQ=255)
                                                                                                       |                                                                                                    
GTGTCTGCCACGACGCCCTGCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTTACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397199‑1397399

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG