Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,318 A→G 4.2% G400G (GGA→GGG RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3180A→G 4.2% 22.8 ‑3.7 ‑0.3334G400G (GGA→GGGRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/12):  ref base (148/172):  total (150/184)
Fisher's exact test strand distribution p-value = 2.54e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.47e-01

Only 100 of 356 total aligned reads displayed.

GCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397218‑1397418
                                                                                                      |                                                                                                     
gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                                                       <  1:864988/101‑1 (MQ=255)
gCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGa                                                                                                       >  2:1792435/1‑101 (MQ=255)
   ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                                                    <  2:1718635/101‑1 (MQ=255)
   ccAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCg                                                                                                    >  1:361726/1‑101 (MQ=255)
    cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAgggg                                                                                                   >  2:4128312/1‑98 (MQ=255)
    cAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGt                                                                                                   <  2:2488289/101‑1 (MQ=255)
      gATCCGCGCCGTGGCGCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                                                 <  1:7026641/101‑1 (MQ=255)
      gATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTgg                                                                                                 <  1:3012913/101‑1 (MQ=255)
         ccGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATc                                                                                              <  1:6222569/101‑1 (MQ=255)
          cgcgCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCg                                                                                             >  1:4646763/1‑101 (MQ=255)
            gcacGTCGCTCCCCCCGCGGGGCCGTGGATTGAACCAATGGGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                                           <  2:4288230/98‑1 (MQ=0)
            cgcCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaa                                                                                           <  2:2722899/101‑1 (MQ=255)
              ccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAAc                                                                                         <  1:1701768/101‑1 (MQ=255)
               cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                                        >  1:5249334/1‑101 (MQ=255)
                gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                                       >  1:4956331/1‑101 (MQ=255)
                  cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCt                                                                                     >  2:3504617/1‑101 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGaaagaggtt                                                                                    >  1:3337490/1‑95 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  1:2664581/1‑101 (MQ=255)
                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCtt                                                                                    >  2:5638820/1‑101 (MQ=255)
                    cTCCCCCCGCGGGGGCGTGGATCGAAAC.CCCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCttt                                                                                   <  1:3309317/101‑1 (MQ=255)
                       cccccGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACa                                                                               <  1:4090185/101‑1 (MQ=255)
                        ccccGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCCCCGGAGCCGGGATCGAAACGGCTTT.ACAg                                                                              >  2:86111/1‑101 (MQ=39)
                          ccGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGcc                                                                            <  2:4508244/101‑1 (MQ=255)
                            gcgGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCtt                                                                          >  1:6977224/1‑101 (MQ=255)
                             cgGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                         <  2:4918865/101‑1 (MQ=255)
                             cgGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                         <  1:999544/101‑1 (MQ=255)
                              gggggCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                        <  1:1723515/101‑1 (MQ=255)
                              gggggCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                        <  2:2113790/101‑1 (MQ=255)
                               ggggCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTCT.ACAGCCTTcgc                                                                       <  1:5054991/101‑1 (MQ=255)
                                gggCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                      <  2:5579068/101‑1 (MQ=255)
                                gggCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGGGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                      >  1:6777058/1‑101 (MQ=255)
                                 ggCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCgcgc                                                                     >  2:5786838/1‑101 (MQ=255)
                                   cGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                   >  1:5609368/1‑101 (MQ=255)
                                   cGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                   >  2:621687/1‑101 (MQ=255)
                                    gTGGATCGAAAC.ACCGCGCACGCGCGAGCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccc                                                                  <  2:1823939/101‑1 (MQ=255)
                                    gTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccc                                                                  >  2:3800255/1‑101 (MQ=255)
                                     tGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 <  2:3649596/101‑1 (MQ=255)
                                     tGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 >  2:6743076/1‑101 (MQ=255)
                                     tGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                 <  1:3971088/101‑1 (MQ=255)
                                      ggATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                >  2:1265689/1‑101 (MQ=255)
                                      ggATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                >  1:1266413/1‑101 (MQ=255)
                                       gATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               <  1:786515/101‑1 (MQ=255)
                                       gATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               >  1:600075/1‑101 (MQ=255)
                                       gATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                               >  2:5472968/1‑101 (MQ=255)
                                          cGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                            >  1:3468155/1‑101 (MQ=255)
                                             aaC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                         >  2:2443463/1‑101 (MQ=255)
                                                 acCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgga                                                      >  1:6605257/1‑101 (MQ=255)
                                                  ccGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                     <  1:3757923/101‑1 (MQ=255)
                                                    gcgcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                   <  1:4428629/101‑1 (MQ=255)
                                                      gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCg                                                 >  1:2372810/1‑101 (MQ=255)
                                                       cACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGc                                                <  1:4341028/101‑1 (MQ=255)
                                                        aCGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                                               <  2:2664485/101‑1 (MQ=255)
                                                         cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                                              <  2:6346617/101‑1 (MQ=255)
                                                          gcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc                                             >  1:4738896/1‑101 (MQ=255)
                                                            gcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccc                                           <  1:6420389/101‑1 (MQ=255)
                                                              gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGTCCCACGCCAGACGGACGTCGCTcccccc                                         <  2:3380833/101‑1 (MQ=255)
                                                              gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                         >  2:3098238/1‑101 (MQ=255)
                                                               aaCGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCcg                                        >  1:4558727/1‑101 (MQ=255)
                                                                    c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                   >  1:5404914/1‑101 (MQ=255)
                                                                       tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc                                 >  2:1617121/1‑101 (MQ=255)
                                                                        cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCTCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg                                >  1:1607824/1‑101 (MQ=255)
                                                                        cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg                                >  2:1961618/1‑101 (MQ=255)
                                                                         aGCCCCGCGAACGTCGCGCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGt                               <  2:71307/101‑1 (MQ=255)
                                                                           ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg                             <  2:736184/101‑1 (MQ=255)
                                                                           ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg                             >  2:3718346/1‑101 (MQ=255)
                                                                              cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                          <  2:6913687/101‑1 (MQ=255)
                                                                                 gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                       >  1:6637196/1‑101 (MQ=255)
                                                                                  gaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                      <  2:2197433/100‑1 (MQ=255)
                                                                                  aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                      >  2:1406421/1‑101 (MQ=255)
                                                                                  aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                      <  1:4144467/101‑1 (MQ=255)
                                                                                   aCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                     >  1:3090214/1‑101 (MQ=255)
                                                                                    cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                    >  1:2026488/1‑100 (MQ=255)
                                                                                     gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCATACGGACGTCGCTCCCCCCGCGCGGGCGTGGATCGAAActc                   >  2:4761598/1‑101 (MQ=255)
                                                                                     gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                   >  1:1439109/1‑101 (MQ=255)
                                                                                      tCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAActct                  <  1:1017254/101‑1 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 <  2:3250910/101‑1 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 >  1:4864264/1‑101 (MQ=255)
                                                                                       cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                 <  2:854132/101‑1 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                >  2:6650804/1‑101 (MQ=255)
                                                                                        gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                <  1:573745/101‑1 (MQ=255)
                                                                                         cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa               <  2:949341/101‑1 (MQ=255)
                                                                                         cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa               >  2:561785/1‑101 (MQ=255)
                                                                                         cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa               <  2:4577831/101‑1 (MQ=255)
                                                                                          tCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa              >  2:2964817/1‑101 (MQ=255)
                                                                                          tCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa              <  2:2032035/101‑1 (MQ=255)
                                                                                          tCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa              >  1:3794759/1‑101 (MQ=255)
                                                                                             ccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATgg           <  2:5873131/101‑1 (MQ=255)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          <  2:6030774/101‑11 (MQ=2)
                                                                                              cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc          <  1:2162198/101‑11 (MQ=2)
                                                                                                cgcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGata        <  1:1982107/101‑1 (MQ=255)
                                                                                                 gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat       <  2:3987591/101‑1 (MQ=255)
                                                                                                 gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat       <  1:4288300/101‑1 (MQ=255)
                                                                                                 gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCACGCGGGGGCGTGGATCGAAACTCTTCAATGGatat       <  1:2544981/101‑1 (MQ=255)
                                                                                                 gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCGTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat       <  2:5249068/101‑1 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      <  2:5072163/101‑1 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      >  2:2850445/1‑101 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      <  2:1450730/101‑1 (MQ=255)
                                                                                                  cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg      <  1:2866054/101‑1 (MQ=255)
                                                                                                    ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc    >  2:6543979/1‑101 (MQ=255)
                                                                                                      aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa  >  2:5432256/1‑101 (MQ=255)
                                                                                                      |                                                                                                     
GCACCAGATCCGCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.ACCGCGCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAA  >  NC_014034/1397218‑1397418

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG