Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,333 G→T 1.4% T405T (ACG→ACT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,3330G→T 1.4% 2.9 0.0 ‑0.6363T405T (ACG→ACTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (175/179):  total (177/186)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.52e-01

Only 100 of 387 total aligned reads displayed.

CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAT  >  NC_014034/1397233‑1397433
                                                                                                      |                                                                                                     
cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACg                                                                                                       >  2:3179529/1‑101 (MQ=255)
 gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACgg                                                                                                      >  1:71308/1‑101 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                                                     <  2:1692843/101‑1 (MQ=255)
  tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGc                                                                                                     >  2:4353285/1‑101 (MQ=255)
   cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTGCCCCCGCGGGGGCGCGGATGGAAAGGGCt                                                                                                    >  1:2283363/1‑101 (MQ=37)
       ccccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.Ac                                                                                               <  2:5597889/101‑1 (MQ=255)
        cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACa                                                                                              >  2:120715/1‑101 (MQ=255)
        cccccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACa                                                                                              <  1:3632994/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGc                                                                                            <  1:167993/101‑1 (MQ=255)
          cccGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGc                                                                                            >  1:3987766/1‑101 (MQ=255)
           ccGCGGGGGCGTGGATCGAAACAATGCGGCGCGGGCCTATGGCTGACGGACCCGC..CCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgtgcgcgcc                                                                                            <  1:4260198/101‑10 (MQ=0)
             gcgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCtt                                                                                         <  1:430923/101‑1 (MQ=255)
              cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                                        <  1:2151893/101‑1 (MQ=255)
              cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTc                                                                                        <  2:4629331/101‑1 (MQ=255)
              cgGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.AAAGCCTTc                                                                                        >  2:6092485/1‑101 (MQ=255)
               gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                                       >  2:4172164/1‑101 (MQ=255)
               gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                                       <  2:5961788/101‑1 (MQ=255)
               gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                                       <  2:2881055/101‑1 (MQ=255)
               gggggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcg                                                                                       >  2:5226673/1‑101 (MQ=255)
                 gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                                     >  1:6185640/1‑101 (MQ=255)
                 gggCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTcgcg                                                                                     >  1:970434/1‑101 (MQ=255)
                    cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                                  >  1:5609368/1‑101 (MQ=255)
                    cGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGccc                                                                                  <  1:2701637/101‑1 (MQ=255)
                     gTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGcccc                                                                                 >  2:3800255/1‑101 (MQ=255)
                      tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                                >  1:2572611/1‑101 (MQ=255)
                      tGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCa                                                                                <  2:3649596/101‑1 (MQ=255)
                       ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                               <  2:1308071/101‑1 (MQ=255)
                       ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                               >  1:1266413/1‑101 (MQ=255)
                       ggATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCAc                                                                               <  1:2983871/101‑1 (MQ=255)
                        gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                                              >  2:4557352/1‑101 (MQ=255)
                        gATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACg                                                                              >  1:600075/1‑101 (MQ=255)
                          tCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGcc                                                                            <  1:3086659/101‑1 (MQ=255)
                           cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                                           >  1:436599/1‑101 (MQ=255)
                           cGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCa                                                                           >  1:3468155/1‑101 (MQ=255)
                             aaaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAga                                                                         >  2:2528051/1‑101 (MQ=255)
                              aaCACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgac                                                                        >  2:5752643/1‑101 (MQ=255)
                                 acCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacgga                                                                     >  1:6605257/1‑101 (MQ=255)
                                  ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                                    <  1:4839735/101‑1 (MQ=255)
                                  ccGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggac                                                                    <  2:2786033/101‑1 (MQ=255)
                                   cgc.gCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAgacggacg                                                                   <  2:1832656/101‑1 (MQ=255)
                                    gc.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGt                                                                  <  1:4428629/101‑1 (MQ=255)
                                     c.gcACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTc                                                                 <  1:2460742/101‑1 (MQ=255)
                                         ccgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCt                                                              <  1:4865569/100‑1 (MQ=255)
                                          cgcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTc                                                             <  2:6329740/101‑1 (MQ=255)
                                           gcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc                                                            <  2:1592097/101‑1 (MQ=255)
                                           gcgcgAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcc                                                            >  1:6788199/1‑101 (MQ=255)
                                               gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                        >  2:2251706/1‑101 (MQ=255)
                                               gAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTcccccc                                                        >  1:3219232/1‑101 (MQ=255)
                                                     c.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                                  >  1:5404914/1‑101 (MQ=255)
                                                     c.GTCAGCCCCGCGAACGCCGCCCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCgggg                                                  <  2:3066075/101‑1 (MQ=255)
                                                        tCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGc                                                <  2:2183731/101‑1 (MQ=255)
                                                         cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg                                               >  2:5049188/1‑101 (MQ=255)
                                                         cAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCg                                               >  1:5123119/1‑101 (MQ=255)
                                                            ccccGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTgg                                            >  2:3718346/1‑101 (MQ=255)
                                                               cgcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATc                                         <  2:6913687/101‑1 (MQ=255)
                                                                gcgAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCg                                        <  2:4865981/101‑1 (MQ=255)
                                                                  gAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                      <  1:3769324/101‑1 (MQ=255)
                                                                   acgcgcGCTCCCCCCGCGGGAGCGTGGACCGACACTCCTTG.ACAGCCTCCGCGCCCCACGCCACACGGACGCCGCTCCCCCCGCGGGGGCGTGGATCGaca                                     <  2:3337374/96‑3 (MQ=25)
                                                                   aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                     >  2:6526505/1‑101 (MQ=255)
                                                                   aaCGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                     >  2:2674062/1‑101 (MQ=255)
                                                                     cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                   >  1:4651669/1‑101 (MQ=255)
                                                                     cGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                   >  1:2026488/1‑100 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                  >  2:6648141/1‑101 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                  >  1:1439109/1‑101 (MQ=255)
                                                                      gTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                  >  1:503078/1‑101 (MQ=255)
                                                                       tCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAActct                                 <  1:1017254/101‑1 (MQ=255)
                                                                        cGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCtt                                <  2:854132/101‑1 (MQ=255)
                                                                         gCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTc                               <  1:573745/101‑1 (MQ=255)
                                                                          cTCCCCCCGCGGGAGCGTGGATCTAAACGGCTTT.ACAGCCTTCCCGCCCCACGCCAGACGGACGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                              <  1:770962/101‑1 (MQ=255)
                                                                          cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                              <  2:949341/101‑1 (MQ=255)
                                                                          cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                              <  2:804824/101‑1 (MQ=255)
                                                                          cTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCa                              >  2:7018498/1‑101 (MQ=255)
                                                                           tCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaa                             <  2:2032035/101‑1 (MQ=255)
                                                                            ccccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCaag                            >  2:6695666/1‑100 (MQ=255)
                                                                              ccccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATgg                          <  2:5873131/101‑1 (MQ=255)
                                                                               cccGCGGGGGCGTGGATCGAAACAGCTTTCAATGGGGGCGGGCGCCGGGCCA.ACCTATGTCGCTCACCCCGCGGGGGCGTGGATCGAAActgtgccggttc                         <  2:1963088/101‑11 (MQ=2)
                                                                               cccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGa                         >  2:861822/1‑101 (MQ=255)
                                                                                ccGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGat                        >  2:6855614/1‑101 (MQ=255)
                                                                                  gcgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGatat                      <  2:3987591/101‑1 (MQ=255)
                                                                                   cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg                     <  1:4113422/101‑1 (MQ=255)
                                                                                   cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg                     >  2:5626764/1‑101 (MQ=255)
                                                                                   cgGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATg                     <  2:1450730/101‑1 (MQ=255)
                                                                                    gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                    >  1:1784195/1‑101 (MQ=255)
                                                                                    gggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATgg                    >  2:1796627/1‑101 (MQ=255)
                                                                                     ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc                   >  2:729325/1‑101 (MQ=255)
                                                                                     ggAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGc                   <  1:7018872/101‑1 (MQ=255)
                                                                                       aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa                 >  2:5432256/1‑101 (MQ=255)
                                                                                       aGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCaa                 <  1:337980/101‑1 (MQ=255)
                                                                                           tGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAAcc             >  1:6283742/1‑101 (MQ=255)
                                                                                           tGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAAcc             >  1:6468572/1‑101 (MQ=255)
                                                                                             gATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGt           <  1:200972/101‑1 (MQ=255)
                                                                                              aTCGAAACGGCTTT.ACAGCCTTCGCGCCCCATGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTc          <  1:4557591/101‑1 (MQ=255)
                                                                                                cGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcg        >  2:549476/1‑101 (MQ=255)
                                                                                                 gAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcgc       >  2:6790030/1‑101 (MQ=255)
                                                                                                  aaaCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcgcg      <  2:2959558/101‑1 (MQ=255)
                                                                                                  aaaCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCcgcg      >  1:385471/1‑101 (MQ=255)
                                                                                                   aaCGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGa     >  2:2454512/1‑101 (MQ=255)
                                                                                                     cGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACa   <  1:6667638/101‑1 (MQ=255)
                                                                                                      ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt  >  2:3497853/1‑101 (MQ=255)
                                                                                                      ggCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAt  <  2:3428979/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCGC.GCACGCGCGAACGGC.GTCAGCCCCGCGAACGTCGCTCCCCCCGCGGGAGCGTGGATCGAAACGGCTTT.ACAGCCTTCGCGCCCCACGCCAGACGGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACAT  >  NC_014034/1397233‑1397433

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG