Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,477 C→T 12.3% T453T (ACC→AAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4770C→T12.3% 132.3 ‑0.1 0.0412T453T (ACC→ACTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/22):  ref base (210/150):  total (240/172)
Fisher's exact test strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 454 total aligned reads displayed.

CCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCC  >  NC_014034/1397377‑1397577
                                                                                                      |                                                                                                         
cccGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.acc                                                                                                           <  2:5337913/101‑1 (MQ=255)
  cgcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.accac                                                                                                         <  1:993566/101‑1 (MQ=255)
  cgcgGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATc.accac                                                                                                         <  2:6787845/101‑1 (MQ=255)
   gcgGGGGCGTGGGTCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACg                                                                                                        >  1:6269277/1‑101 (MQ=255)
     gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                                      >  2:245520/1‑101 (MQ=255)
     gggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCa                                                                                                      <  2:2601136/101‑1 (MQ=255)
      ggggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCaa                                                                                                     >  1:3914009/1‑101 (MQ=255)
        ggCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.cc                                                                                                  <  1:1427082/101‑1 (MQ=255)
        ggCGTGGATCGAAACTCTTCAATGGAATTGGGAAAACCGTCCGCGACATGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.TCCACGCAA.cc                                                                                                  <  2:697292/101‑1 (MQ=37)
         gCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCg                                                                                                 <  2:5404642/101‑1 (MQ=255)
           gTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGcc                                                                                               >  1:534205/1‑101 (MQ=255)
             ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                             >  2:2199671/1‑101 (MQ=255)
             ggATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCt                                                                                             >  2:521290/1‑101 (MQ=255)
              gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTg                                                                                            >  2:86262/1‑101 (MQ=255)
              gATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTg                                                                                            >  1:2667736/1‑101 (MQ=255)
                tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                                          >  1:6125087/1‑101 (MQ=255)
                tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                                          >  2:4187914/1‑101 (MQ=255)
                 cGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcac                                                                                         >  2:4326089/1‑101 (MQ=255)
                   taaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                       <  1:5992710/100‑1 (MQ=255)
                   aaaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacca                                                                                       >  2:2040017/1‑101 (MQ=255)
                    aaCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcaccaa                                                                                      >  2:5992405/1‑100 (MQ=255)
                     aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                     <  1:6951342/101‑1 (MQ=255)
                     aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                     <  1:1758260/101‑1 (MQ=255)
                      ctctTCAATGGGTATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                    >  2:4740497/1‑101 (MQ=255)
                        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...Gt                                                                               >  2:3206767/1‑101 (MQ=255)
                        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...Gt                                                                               >  1:3342470/1‑101 (MQ=255)
                        ctTCAATGGAGATGGCAAAACCGGCCGCGACATGTCGCCCCCCCAGCGGGGGCGTGGATCGAAACCCCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...Gt                                                                               <  1:6437337/101‑1 (MQ=38)
                         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTc                                                                              >  1:2866665/1‑101 (MQ=255)
                         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTc                                                                              <  1:4784360/101‑1 (MQ=255)
                         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTc                                                                              <  1:6733054/101‑1 (MQ=255)
                         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCGGCACCACCG...GTc                                                                              <  1:6356429/101‑1 (MQ=255)
                           cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGc                                                                            >  2:5515729/1‑101 (MQ=255)
                            aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCt                                                                           >  1:3088818/1‑101 (MQ=255)
                            aaTGGACATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCt                                                                           <  2:758771/101‑1 (MQ=255)
                              tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.cc                                                                        <  1:5783717/101‑1 (MQ=255)
                                gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.cccc                                                                      >  1:5671074/1‑101 (MQ=255)
                                 atatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.ccccc                                                                     <  2:7051313/101‑1 (MQ=255)
                                  tatGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.cccccc                                                                    >  1:3967273/1‑101 (MQ=255)
                                   atGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCcg                                                                   >  2:5846690/1‑101 (MQ=255)
                                    tGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCcgc                                                                  <  1:2139083/101‑1 (MQ=255)
                                     ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCcgcg                                                                 >  2:4870127/1‑101 (MQ=255)
                                       cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCggg                                                               <  1:3519026/101‑1 (MQ=255)
                                        aaaaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCgggg                                                              <  1:3578413/101‑1 (MQ=255)
                                          aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGc                                                            >  2:6640592/1‑101 (MQ=255)
                                          aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGc                                                            <  1:10128/101‑1 (MQ=255)
                                            ccGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGt                                                          <  2:6103343/101‑1 (MQ=255)
                                             cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTg                                                         <  2:1212178/101‑1 (MQ=255)
                                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                        >  1:1813906/1‑101 (MQ=255)
                                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                        <  1:4536472/101‑1 (MQ=255)
                                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTgg                                                        <  2:1607763/101‑1 (MQ=255)
                                                ccGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGAt                                                      <  2:3921136/101‑1 (MQ=255)
                                                 gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGGGGa                                                       >  1:4706102/9‑101 (MQ=0)
                                                  gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                    <  2:5434877/101‑1 (MQ=255)
                                                    gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                  >  2:6342075/1‑101 (MQ=255)
                                                    gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                  >  1:6903267/1‑101 (MQ=255)
                                                    accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGGGGAtag                                                    >  2:2979050/6‑99 (MQ=0)
                                                      cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaac                                                <  1:2454606/101‑1 (MQ=255)
                                                        tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacaa                                              <  1:2056132/101‑1 (MQ=255)
                                                        cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaac                                                <  1:5462309/100‑1 (MQ=2)
                                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCG.TGCGC.GCCATGAAT.TCATCGAGCACCATCGAGCGTCGCT.CCCCCCGCGGGGGCGTGGATCGAaaca                                               >  2:1753864/1‑101 (MQ=2)
                                                            gCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtg                                            >  1:3633042/1‑97 (MQ=2)
                                                              tCCGCCCGCGGGGGCGTGGATCTAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                          <  1:7119083/101‑7 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtc                                          <  1:327976/101‑7 (MQ=2)
                                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggggtc                                          >  2:6484113/1‑95 (MQ=2)
                                                               ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                         <  1:162487/101‑8 (MQ=2)
                                                               ccccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtcc                                         >  2:4558066/1‑94 (MQ=2)
                                                                cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                        <  2:3537101/101‑9 (MQ=2)
                                                                 ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCGGCGGGGGCGTGGATCGAAACAACCCGATCAt                                     >  1:1153384/1‑101 (MQ=255)
                                                                 ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                     <  2:6268954/101‑1 (MQ=255)
                                                                  cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC....GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                      >  2:193310/1‑91 (MQ=2)
                                                                    cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                  <  2:2745487/101‑1 (MQ=255)
                                                                      cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                >  2:239200/1‑101 (MQ=255)
                                                                       gggggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCg                               <  2:4402071/101‑1 (MQ=255)
                                                                         gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                             <  2:2343124/101‑1 (MQ=255)
                                                                          ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcgt                            >  1:1536991/1‑99 (MQ=255)
                                                                           gCGTGGATCGAAACCCCG.TCCTC.ACCACGCAA.CCCCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                           <  1:435121/101‑1 (MQ=255)
                                                                           gCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                           <  1:906174/101‑1 (MQ=255)
                                                                            cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                          <  2:1152650/101‑1 (MQ=255)
                                                                              tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGACCGCCTCAgc                        >  1:5081410/1‑101 (MQ=255)
                                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                       >  2:288276/1‑101 (MQ=255)
                                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                       >  2:3251479/1‑101 (MQ=255)
                                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCACGGCCCGCCTCAgcg                       <  2:1383374/101‑1 (MQ=255)
                                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCAGATCATGGCCCCCCTCAgcg                       >  1:407371/1‑101 (MQ=255)
                                                                                gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                      >  2:3741373/1‑101 (MQ=255)
                                                                                gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                      <  2:3049428/101‑1 (MQ=255)
                                                                                gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                      >  1:6628367/1‑101 (MQ=255)
                                                                                gATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCCGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcgc                      >  2:405983/1‑101 (MQ=255)
                                                                                 aTCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgcc                     <  2:3999934/101‑1 (MQ=255)
                                                                                   cGAAACACCG.TCATC.CCGACGCAA.CCGCCCTGCACCACCG...GACGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCAGATCATGGCCCGCCGCAGcgccgc                   >  2:5671130/1‑101 (MQ=38)
                                                                                   cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                   >  1:4906155/1‑101 (MQ=255)
                                                                                   cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                   >  1:2572259/1‑101 (MQ=255)
                                                                                   cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                   <  2:3468031/101‑1 (MQ=255)
                                                                                    gAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCcgcg                  <  1:5193074/101‑1 (MQ=255)
                                                                                               catc.aCCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc        >  1:1227324/1‑101 (MQ=255)
                                                                                                 tc.aCCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTc      >  1:4996330/1‑101 (MQ=255)
                                                                                                 tc.aCCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTc      >  2:378529/1‑101 (MQ=255)
                                                                                                   ccccacGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc     <  2:508273/99‑1 (MQ=255)
                                                                                                    cccacGCAA.CCGCCCTGCACCACCG...GTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGCCGCTcc     <  1:5517534/100‑1 (MQ=255)
                                                                                                     ccacGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc   <  2:4621514/101‑1 (MQ=255)
                                                                                                      cacGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc  <  1:777748/101‑1 (MQ=255)
                                                                                                      |                                                                                                         
CCCGCGGGGGCGTGGATCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCG...GTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCC  >  NC_014034/1397377‑1397577

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG