Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,493 A→T 13.3% T459S (ACC→TCG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,4930A→T13.3% 149.1 ‑0.1 ‑1.7391T459S (ACC→TCC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (29/23):  ref base (192/145):  total (221/170)
Fisher's exact test strand distribution p-value = 8.81e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.80e-02

Only 100 of 441 total aligned reads displayed.

TCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1397393‑1397593
                                                                                                       |                                                                                                     
tCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGca                                                                                                       >  1:6125087/1‑101 (MQ=255)
  gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacc                                                                                                     >  2:3089637/1‑101 (MQ=255)
  gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacc                                                                                                     <  2:1349040/101‑1 (MQ=255)
  gAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGcacc                                                                                                     >  1:643620/1‑101 (MQ=255)
     aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                                  <  1:6951342/101‑1 (MQ=255)
     aCTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCaccacc                                                                                                  <  1:1758260/101‑1 (MQ=255)
      ctctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCg                                                                                                 <  2:3672366/101‑1 (MQ=255)
        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                               <  2:290777/101‑1 (MQ=255)
        ctTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGt                                                                                               >  2:1712845/1‑101 (MQ=255)
         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                              <  1:6733054/101‑1 (MQ=255)
         ttCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTc                                                                                              >  1:2866665/1‑101 (MQ=255)
           cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                                            >  1:5127065/1‑101 (MQ=255)
           cAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGc                                                                                            >  2:1140941/1‑101 (MQ=255)
            aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGcc                                                                                           >  2:2452087/1‑100 (MQ=255)
            aaTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCt                                                                                           >  1:2586339/1‑101 (MQ=255)
             aTGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.c                                                                                         <  2:5421142/101‑1 (MQ=255)
              tGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.cc                                                                                        <  1:5783717/101‑1 (MQ=255)
               ggATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.ccc                                                                                       >  1:1454355/1‑101 (MQ=255)
               ggATATGGCAAAACCGACCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.ccc                                                                                       >  2:1183259/1‑101 (MQ=255)
                gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.cccc                                                                                      >  1:5671074/1‑101 (MQ=255)
                gATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.cccc                                                                                      >  1:4653416/1‑101 (MQ=255)
                     ggCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCcgcg                                                                                 >  1:392166/1‑101 (MQ=255)
                       cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCggg                                                                               >  1:5973596/1‑101 (MQ=255)
                       cAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCggg                                                                               <  2:4864020/101‑1 (MQ=255)
                          aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGc                                                                            >  2:6640592/1‑101 (MQ=255)
                          aaCCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGc                                                                            <  1:10128/101‑1 (MQ=255)
                             cGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTg                                                                         >  2:3475632/1‑101 (MQ=255)
                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTgg                                                                        >  1:1813906/1‑101 (MQ=255)
                              gTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTgg                                                                        >  1:5061755/1‑101 (MQ=255)
                               ccgataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTg                                                                         <  1:4788008/91‑1 (MQ=0)
                                 gataccgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGa                                                                       >  1:2674346/9‑101 (MQ=0)
                                  gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                                    <  2:6994907/101‑1 (MQ=255)
                                  gcgACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCg                                                                    >  2:6906374/1‑101 (MQ=255)
                                    gACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                  >  1:6903267/1‑101 (MQ=255)
                                    accgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGGGGATCg                                                                    >  2:5024722/6‑101 (MQ=0)
                                      cgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGaa                                                                  >  1:5263772/4‑101 (MQ=2)
                                       gcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGaaa                                                                 <  2:211833/99‑1 (MQ=2)
                                       aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAaaca                                                               <  2:6400307/101‑1 (MQ=255)
                                        cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaac                                                                <  1:5462309/100‑1 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacg                                                               >  2:3945302/1‑100 (MQ=2)
                                         gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacaac                                                             >  1:3115698/1‑101 (MQ=255)
                                          tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAAcc                                                            <  1:7044645/101‑1 (MQ=255)
                                              tCCCCCCGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggggtc                                                          >  2:6484113/1‑95 (MQ=2)
                                               ccccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATc                                                       <  1:6189770/101‑1 (MQ=255)
                                                cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtcca                                                        <  2:4452985/101‑9 (MQ=2)
                                                cccccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGAGCGTGGATCGAaacggtgtcca                                                        >  1:327743/1‑93 (MQ=2)
                                                 ccccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                     <  2:6268954/101‑1 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACATCGATGATCAAATCCGCAATCGGCCGGGCTCGATC.GTCGCT.CCCCCCGCGGGGGCGTGGATCGAaacggtgtccagc                                                      <  1:3528053/101‑11 (MQ=2)
                                                  cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                    <  1:1671771/101‑1 (MQ=255)
                                                  cccGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                    <  2:2866556/101‑1 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                  <  1:2040097/101‑1 (MQ=255)
                                                    cgcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                  <  1:6320213/101‑1 (MQ=255)
                                                     gcgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGcc                                                 <  1:6544310/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                >  1:876238/1‑101 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                <  2:4558488/101‑1 (MQ=255)
                                                      cgGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGccc                                                >  2:5341879/1‑101 (MQ=255)
                                                         gggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGcc                                             >  1:4711007/1‑101 (MQ=255)
                                                          ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                            <  2:4027888/101‑1 (MQ=255)
                                                          ggCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCt                                            <  2:4646524/101‑1 (MQ=255)
                                                           gCGTGGATCGAAACCCCG.TCCTC.ACCACGCAA.CCCCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTc                                           <  1:435121/101‑1 (MQ=255)
                                                            cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                          >  1:1160417/1‑101 (MQ=255)
                                                            cGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCa                                          <  1:1511626/101‑1 (MQ=255)
                                                              tGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgc                                        >  2:788955/1‑101 (MQ=255)
                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                                       <  2:6042473/101‑1 (MQ=255)
                                                               ggATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAgcg                                       >  2:3251479/1‑101 (MQ=255)
                                                                   tgAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGcgccgc                                   <  2:4346323/100‑1 (MQ=255)
                                                                   cGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGCTCATGGCCCGCCTCAGcgccgc                                   >  1:3978364/1‑101 (MQ=255)
                                                                    gAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGCTCATGGCCCGCCTCAGCGCcgcg                                  >  2:7085210/1‑101 (MQ=255)
                                                                     aaaCACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCgcgc                                 >  2:2676869/1‑101 (MQ=255)
                                                                           cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt                           <  1:2470117/101‑1 (MQ=255)
                                                                           cG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGt                           <  1:729345/101‑1 (MQ=255)
                                                                            g.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                          <  2:1514918/101‑1 (MQ=255)
                                                                            g.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                          >  2:5841289/1‑101 (MQ=255)
                                                                            g.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTc                          >  2:5978025/1‑101 (MQ=255)
                                                                               catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc                        >  1:1227324/1‑101 (MQ=255)
                                                                               catc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGc                        >  1:989294/1‑101 (MQ=255)
                                                                                atc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTAGCt                       >  2:2267810/1‑101 (MQ=255)
                                                                                 tc.aCCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTc                      >  2:378529/1‑101 (MQ=255)
                                                                                 ccgatctcGCAA.CCGCCNTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCt                       <  2:1872467/94‑1 (MQ=255)
                                                                                  c.accacGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCGGCCTCAGCGCCGCGCTGACGCCGCTcc                     >  2:199076/1‑101 (MQ=255)
                                                                                  c.accacGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                     >  2:5515133/1‑101 (MQ=255)
                                                                                  c.accacGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcc                     >  1:4920225/1‑101 (MQ=255)
                                                                                    cccacGCAA.CCGCCCTGCACCACCGGTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGCCGCTcc                     <  1:5517534/100‑1 (MQ=255)
                                                                                     ccacGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccc                   >  2:3012516/1‑101 (MQ=255)
                                                                                      cacGCAA.CCGCCCTGCACCCCCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTccccc                  <  1:3309724/101‑1 (MQ=255)
                                                                                       acGCAA.CCGCCCTGCCCCGCCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTcccccc                 <  1:2264302/101‑1 (MQ=255)
                                                                                        cGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcg                <  2:40629/101‑1 (MQ=255)
                                                                                         gCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc               >  1:3699672/1‑101 (MQ=255)
                                                                                          gccgccGCCCGGCCCCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCcgc               <  2:4212504/97‑1 (MQ=255)
                                                                                           aa.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg             >  1:3612383/1‑101 (MQ=255)
                                                                                           aa.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg             <  2:4800391/101‑1 (MQ=255)
                                                                                           aa.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCgg             >  2:2390115/1‑101 (MQ=255)
                                                                                            a.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCggg            <  2:5590338/101‑1 (MQ=255)
                                                                                                gCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGc         >  2:5287233/1‑101 (MQ=255)
                                                                                                 cccTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCg        <  1:3472755/101‑1 (MQ=255)
                                                                                                   cTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg      >  2:6207329/1‑101 (MQ=255)
                                                                                                   cTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTg      >  1:4074675/1‑101 (MQ=255)
                                                                                                    tGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTgg     <  2:4160696/101‑1 (MQ=255)
                                                                                                      caccacCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGAt   <  2:3896283/101‑1 (MQ=255)
                                                                                                       accaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATc  >  1:2110607/1‑101 (MQ=255)
                                                                                                       |                                                                                                     
TCGAAACTCTTCAATGGATATGGCAAAACCGTCCGCGACATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCG.TCATC.ACCACGCAA.CCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGCCCGCCTCAGCGCCGCGCTGACGTCGCTCCCCCCGCGGGGGCGTGGATC  >  NC_014034/1397393‑1397593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG