Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,539 T→C 14.3% I474T (ATC→GCC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5390T→C14.3% 143.8 ‑0.5 ‑1.8532I474T (ATC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (28/31):  ref base (207/194):  total (264/268)
Fisher's exact test strand distribution p-value = 5.79e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.56e-02

Only 100 of 557 total aligned reads displayed.

TCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTC  >  NC_014034/1397439‑1397639
                                                                                                    |                                                                                                          
   ccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCGGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                                                                         >  1:1153384/1‑101 (MQ=255)
    cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                                                                        >  2:208294/1‑101 (MQ=255)
    cccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATg                                                                                                        <  1:1671771/101‑1 (MQ=255)
        cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.Gc.c..c                                                                                                >  2:5341879/1‑101 (MQ=255)
           gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGcc                                                                                             <  2:5973292/101‑1 (MQ=255)
            ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGccc                                                                                            >  1:3768636/1‑100 (MQ=255)
            ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCt                                                                                            >  1:3558373/1‑101 (MQ=255)
            ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCt                                                                                            <  2:4027888/101‑1 (MQ=255)
             gCGTGGACCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTc                                                                                           <  2:6059004/101‑1 (MQ=255)
              cGTAGATCGAAACACCGTCATCACCACGCACCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCa                                                                                          <  1:4761838/101‑1 (MQ=255)
               gTGGATCGAAACACCGTCATCACCGCGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAg                                                                                         <  1:1595007/101‑1 (MQ=255)
                tGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCCgc                                                                                        >  1:1731070/1‑101 (MQ=255)
                  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcgc                                                                                      >  2:6249996/1‑101 (MQ=255)
                  gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcgc                                                                                      >  1:6628367/1‑101 (MQ=255)
                   aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGcgcc                                                                                     >  1:5395714/1‑101 (MQ=255)
                    tCGAAACCCCGTCATCACCACGCAACCGCCCTGCTCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGcgccg                                                                                    <  2:4583654/101‑1 (MQ=255)
                     cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGcgccgc                                                                                   >  1:2572259/1‑101 (MQ=255)
                      gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCcgcg                                                                                  <  1:947210/101‑1 (MQ=255)
                      gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCcgcg                                                                                  >  1:3223012/1‑101 (MQ=255)
                      gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCcgcg                                                                                  >  2:6779249/1‑101 (MQ=255)
                       aaaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCgcgc                                                                                 >  2:2676869/1‑101 (MQ=255)
                        aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCt                                                                                >  2:2905658/1‑101 (MQ=255)
                               tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCg                                                                       >  1:7131550/1‑101 (MQ=255)
                                 atcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                     <  1:2921706/101‑1 (MQ=255)
                                   caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..GGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                   >  2:199076/1‑101 (MQ=255)
                                   caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                   >  2:5515133/1‑101 (MQ=255)
                                      cacGCAACCGCCCTGCACCCCCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                <  1:3309724/101‑1 (MQ=255)
                                      cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                >  2:2869582/1‑101 (MQ=255)
                                         gccgccGCCCGGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                             <  2:4212504/97‑1 (MQ=255)
                                           aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                           >  2:2390115/1‑101 (MQ=255)
                                               gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                       <  1:249120/101‑1 (MQ=255)
                                                    tgccgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                  <  2:3576863/92‑1 (MQ=2)
                                                    gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                  >  1:6228860/1‑101 (MQ=255)
                                                     aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  2:7109926/93‑1 (MQ=2)
                                                     aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGATc                                                >  2:5183325/9‑101 (MQ=0)
                                                      ggccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA.CTGACCCTGCAA.C..GGCCTATGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:4917243/5‑101 (MQ=2)
                                                      cccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  1:1234243/100‑1 (MQ=255)
                                                      atcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGAt                                                 <  1:6087492/94‑1 (MQ=0)
                                                      accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                >  1:5224458/1‑101 (MQ=255)
                                                      accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                <  2:1546589/101‑1 (MQ=255)
                                                       gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               <  1:4851079/96‑1 (MQ=0)
                                                       cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                               >  1:6461535/7‑101 (MQ=2)
                                                        aggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:4604102/96‑1 (MQ=2)
                                                         gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  1:5557019/98‑1 (MQ=2)
                                                         gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             >  1:6913257/4‑101 (MQ=2)
                                                         gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                              >  2:3146450/5‑101 (MQ=2)
                                                         cccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCTATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                             <  2:392162/100‑1 (MQ=255)
                                                         accGGTCGCTCCCCCCGCGGGGGCGTGGACCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCAGCGCT.GA.CGTCGCTCCCCCCGCGGGCGAGTGGATCGaa                                             >  2:4743511/1‑101 (MQ=37)
                                                          ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:1073000/98‑1 (MQ=2)
                                                          ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  2:1716426/98‑1 (MQ=2)
                                                          ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                            <  1:1498088/101‑1 (MQ=255)
                                                           tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:4434890/2‑101 (MQ=2)
                                                           cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           >  1:2161777/1‑101 (MQ=255)
                                                           cGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                           <  2:4857397/101‑1 (MQ=255)
                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                          >  1:2292913/1‑100 (MQ=2)
                                                            ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGCGGGCGTGGATCGACACa                                          >  1:5160161/1‑101 (MQ=255)
                                                             gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                         <  1:2790491/101‑1 (MQ=255)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                         >  1:1930463/1‑100 (MQ=2)
                                                              tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                        <  2:643600/101‑1 (MQ=255)
                                                               cccTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                        <  1:1009470/99‑3 (MQ=2)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                        <  1:2225590/101‑3 (MQ=2)
                                                               cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                       <  1:5818317/101‑1 (MQ=255)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACATcga                                      >  2:4328087/1‑99 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                       >  2:1429501/1‑98 (MQ=2)
                                                                gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                      >  2:4998755/1‑101 (MQ=255)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                      <  2:247653/101‑5 (MQ=2)
                                                                 cTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgc                                     <  2:1813842/101‑1 (MQ=255)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAGACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                    <  2:3509291/101‑5 (MQ=2)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                   <  2:4446717/101‑9 (MQ=2)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                    >  1:5925399/1‑97 (MQ=2)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATcgatg                                    >  2:3034409/1‑97 (MQ=2)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                     >  1:4253675/1‑96 (MQ=0)
                                                                  tCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcccg                                    >  2:6574393/1‑101 (MQ=255)
                                                                  cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                    <  1:1476743/100‑5 (MQ=2)
                                                                   ccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatga                                   <  2:4085435/101‑6 (MQ=2)
                                                                   ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                    <  2:3767818/101‑7 (MQ=0)
                                                                    cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                 <  2:1262758/101‑11 (MQ=0)
                                                                    cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                  <  1:5649147/101‑7 (MQ=2)
                                                                      cccGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                <  2:6207149/101‑1 (MQ=255)
                                                                           gggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAAATCCGCGGATCGGCGt                           >  1:6765551/1‑101 (MQ=255)
                                                                            ggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCGCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCTTGGATCGAAAAATCCTCGGATCGGCGTg                          <  1:5068423/101‑1 (MQ=38)
                                                                             cgatctGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                         <  2:6329848/96‑1 (MQ=255)
                                                                               gCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                       <  2:5670784/101‑1 (MQ=255)
                                                                                 gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                     >  2:5833695/1‑101 (MQ=255)
                                                                                 gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                     <  1:3675838/101‑1 (MQ=255)
                                                                                 gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                     <  1:3475768/101‑1 (MQ=255)
                                                                                 gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                     >  2:834570/1‑100 (MQ=255)
                                                                                 gTGGAGCGAAACAGCCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                     <  2:2165794/101‑1 (MQ=255)
                                                                                   ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                   >  1:706735/1‑101 (MQ=255)
                                                                                   ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                   <  1:4028307/101‑1 (MQ=255)
                                                                                   ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGAGCCGCGCT.GA.CGTGGCTCCCCCCGCGGGGGCGTGGATCGAAAAATACGCGGATCGGGGTGAccagcc                   >  2:3294105/1‑101 (MQ=37)
                                                                                   ggATCGAAACAAACCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcg                   >  1:6330173/1‑95 (MQ=255)
                                                                                     aTCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGAcccgcccc                 >  1:4094448/1‑101 (MQ=255)
                                                                                       cGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc               >  2:7048006/1‑101 (MQ=255)
                                                                                        gAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc              >  1:2019029/1‑101 (MQ=255)
                                                                                        caaaCAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc              <  2:6931124/100‑1 (MQ=255)
                                                                                            caacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc          >  2:2243562/1‑101 (MQ=255)
                                                                                            caacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc          >  1:83178/1‑101 (MQ=255)
                                                                                              acCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg        >  1:529826/1‑101 (MQ=255)
                                                                                                ccGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc      <  1:3356019/101‑1 (MQ=255)
                                                                                                    |                                                                                                          
TCCCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTC  >  NC_014034/1397439‑1397639

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG