Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,541 A→C 10.1% M475M (ATG→CAA)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5410A→C10.1% 112.5 ‑1.5 ‑0.5539M475M (ATG→CTG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/28):  ref base (219/203):  total (270/269)
Fisher's exact test strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.51e-01

Only 100 of 565 total aligned reads displayed.

CCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCC  >  NC_014034/1397441‑1397641
                                                                                                     |                                                                                                         
 ccccGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCGGCGGGGGCGTGGATCGAAACAACCCGATCAt                                                                                                          >  1:1153384/1‑101 (MQ=255)
    cgcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                                                                       <  2:1671314/101‑1 (MQ=255)
    cgcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc                                                                                                       <  1:6320213/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c                                                                                                     <  2:2344792/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c                                                                                                     <  1:6544310/101‑1 (MQ=255)
     gcgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c                                                                                                     >  1:1879472/1‑101 (MQ=255)
      cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c..c                                                                                                  >  2:5341879/1‑101 (MQ=255)
      cgGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGc.c..c                                                                                                  <  2:390912/101‑1 (MQ=255)
       gggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..Cg                                                                                                 >  2:117171/1‑101 (MQ=255)
        ggggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGc                                                                                                >  1:4355989/1‑101 (MQ=255)
         gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGcc                                                                                               >  1:4711007/1‑101 (MQ=255)
         gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGcc                                                                                               <  2:5973292/101‑1 (MQ=255)
           gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                             <  2:4199224/101‑1 (MQ=255)
           gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                             <  1:906174/101‑1 (MQ=255)
           gCGTGGACCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTc                                                                                             <  2:6059004/101‑1 (MQ=255)
               ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                         <  2:5126805/101‑1 (MQ=255)
               ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                         >  2:288276/1‑101 (MQ=255)
               ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                         >  2:3251479/1‑101 (MQ=255)
               ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCAGATCATGGC.C..CCCCTCAgcg                                                                                         >  1:407371/1‑101 (MQ=255)
                 aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgcc                                                                                       <  2:3999934/101‑1 (MQ=255)
                 aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGCCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgcc                                                                                       >  1:525587/1‑101 (MQ=255)
                   cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccgc                                                                                     >  1:4906155/1‑101 (MQ=255)
                   cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccgc                                                                                     >  1:3876433/1‑101 (MQ=255)
                    gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                    >  2:3678879/1‑101 (MQ=255)
                       acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.g                                                                                >  2:6755907/1‑101 (MQ=255)
                       acacCCTCCTCCCCACGCAACCGCCCTGCCCCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.g                                                                                <  1:3302163/101‑1 (MQ=38)
                        ccccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.Ga                                                                               <  1:537138/99‑1 (MQ=255)
                        cacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGGTCATGGC.C..CGCCTCCGCGCCGCGCT.Ga                                                                               >  2:2416160/1‑101 (MQ=255)
                               ccgatctcGCAACCGCCNTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                       <  2:1872467/94‑1 (MQ=255)
                                tcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTc                                                                      >  1:4996330/1‑101 (MQ=255)
                                 caccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                     >  2:5515133/1‑101 (MQ=255)
                                  cccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                     <  1:5517534/100‑1 (MQ=255)
                                   ccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                   >  2:3012516/1‑101 (MQ=255)
                                     acGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                 <  1:2674158/101‑1 (MQ=255)
                                       gCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                               <  2:407367/101‑1 (MQ=255)
                                             gCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                         <  1:1705566/101‑1 (MQ=255)
                                              cccTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCg                                                        <  1:3472755/101‑1 (MQ=255)
                                                 ggaacaggatgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                    <  2:6575328/91‑1 (MQ=2)
                                                  gCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                    >  1:6228860/1‑101 (MQ=255)
                                                   aacaggatgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  2:7109926/93‑1 (MQ=2)
                                                    cccaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:1234243/100‑1 (MQ=255)
                                                     tcgagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                  <  1:5206080/95‑1 (MQ=0)
                                                      cgctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  2:4573649/97‑1 (MQ=0)
                                                      cgagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:6029075/6‑101 (MQ=0)
                                                      cgagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                 >  1:7092566/6‑101 (MQ=0)
                                                      aggatgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGGTCgga                                               >  2:2114760/6‑99 (MQ=2)
                                                       gagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                <  2:6254673/97‑1 (MQ=2)
                                                       accGGTCGCT.CCCCCCGCGGGGGCGTGGACCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCAGCGCT.GA.CGTCGCTCCCCCCGCGGGCGAGTGGATCGaa                                               >  2:4743511/1‑101 (MQ=37)
                                                        tgcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                              <  1:3655360/98‑1 (MQ=2)
                                                         tggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                             >  1:2881944/2‑101 (MQ=2)
                                                          ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGACAcc                                            <  1:5596740/101‑2 (MQ=2)
                                                           gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                           <  1:3343300/101‑1 (MQ=255)
                                                            tCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                          <  1:1491076/101‑1 (MQ=2)
                                                            tCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                          >  1:1386124/1‑101 (MQ=2)
                                                            tCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                          <  2:643600/101‑1 (MQ=255)
                                                            gtccccccccccGCGGGGGCGGGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                           <  1:199079/95‑1 (MQ=255)
                                                             cccT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                          <  1:1009470/99‑3 (MQ=2)
                                                              gCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                       <  2:5667150/101‑7 (MQ=2)
                                                              gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCCAAACATcga                                        >  2:4328087/1‑99 (MQ=2)
                                                              gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                         <  1:6403160/101‑4 (MQ=2)
                                                              gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                        >  1:1575361/1‑101 (MQ=255)
                                                              gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                        >  1:2200983/1‑101 (MQ=255)
                                                              gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                        >  2:4998755/1‑101 (MQ=255)
                                                               cT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                       <  2:1496448/101‑4 (MQ=2)
                                                                t.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                      >  1:5925399/1‑97 (MQ=2)
                                                                t.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                      <  1:1659988/101‑5 (MQ=2)
                                                                t.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                      >  1:3533674/1‑97 (MQ=2)
                                                                t.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                       <  1:2126380/101‑6 (MQ=0)
                                                                t.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                      >  1:4578019/1‑101 (MQ=255)
                                                                ccccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                       <  1:3119117/99‑4 (MQ=2)
                                                                  ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGgaaaggggcgaag                                    >  2:4411293/1‑91 (MQ=2)
                                                                  ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGGAAcggggcgaag                                    >  1:6666499/1‑92 (MQ=0)
                                                                  ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                     <  1:2534094/101‑1 (MQ=255)
                                                                  ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                     >  2:1789625/1‑101 (MQ=255)
                                                                   cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                    <  1:5117291/101‑7 (MQ=2)
                                                                     cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                  <  2:2045058/101‑9 (MQ=0)
                                                                     cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                  <  1:4844028/101‑1 (MQ=255)
                                                                     cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                  <  2:6207149/101‑1 (MQ=255)
                                                                        gcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCGGATCgcg                               >  2:2845732/1‑99 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                              <  1:245524/101‑1 (MQ=255)
                                                                         cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                              >  2:3101736/1‑101 (MQ=255)
                                                                            cgatctGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                           <  2:6329848/96‑1 (MQ=255)
                                                                                gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                       >  2:3471904/1‑101 (MQ=255)
                                                                                gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                       >  2:5833695/1‑101 (MQ=255)
                                                                                gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                       >  2:834570/1‑100 (MQ=255)
                                                                                  ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                     >  2:1298682/1‑101 (MQ=255)
                                                                                  ggATCGAAACAACCCGATCAAGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACATCCGCCGATCCGCGGTAcccgcc                     >  2:2204850/1‑101 (MQ=37)
                                                                                   gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                    >  2:4855806/1‑101 (MQ=255)
                                                                                   gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                    <  1:5807529/101‑1 (MQ=255)
                                                                                     tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                  <  1:6953072/101‑1 (MQ=255)
                                                                                      cGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                 >  2:7048006/1‑101 (MQ=255)
                                                                                       gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                >  2:3576291/1‑101 (MQ=255)
                                                                                       caaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                <  2:6931124/100‑1 (MQ=255)
                                                                                         aacaacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa              >  1:4170752/1‑101 (MQ=255)
                                                                                           caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc            <  1:973136/101‑1 (MQ=255)
                                                                                            aacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg           <  2:2667640/101‑1 (MQ=255)
                                                                                              cccGACCATGGC.C..CGCCCCCGCCCCCCGCT.GC.CGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACATCCCCGGATCGGCGTGACCCGCCCCGCCCATCGGt         <  1:2267905/101‑1 (MQ=25)
                                                                                               ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc        <  1:775151/101‑1 (MQ=255)
                                                                                                  aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt     <  1:3739877/101‑1 (MQ=255)
                                                                                                     aTGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGGCCCGCCCATCGGTCGCTccc  >  1:6843526/1‑101 (MQ=255)
                                                                                                     |                                                                                                         
CCCCCGCGGGGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCC  >  NC_014034/1397441‑1397641

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG