Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,550 C→G 4.8% L478V (CTC→GTG)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5500C→G 4.8% 42.2 ‑1.2 0.0527L478V (CTC→GTC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (10/15):  ref base (249/237):  total (265/262)
Fisher's exact test strand distribution p-value = 3.09e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 552 total aligned reads displayed.

GGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGG  >  NC_014034/1397450‑1397650
                                                                                                        |                                                                                                      
gggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGcc                                                                                                        >  1:3587904/1‑101 (MQ=255)
 ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGccc                                                                                                       >  1:3768636/1‑100 (MQ=255)
 ggCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCt                                                                                                       <  2:4646524/101‑1 (MQ=255)
  gCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTc                                                                                                      >  2:2194936/1‑101 (MQ=255)
   cGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCa                                                                                                     >  1:1160417/1‑101 (MQ=255)
      ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcg                                                                                                  >  2:3251479/1‑101 (MQ=255)
      ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcg                                                                                                  >  2:288276/1‑101 (MQ=255)
       gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAgcgc                                                                                                 >  2:6249996/1‑101 (MQ=255)
          tgAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGcgccgc                                                                                              <  2:4346323/100‑1 (MQ=255)
              acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.g                                                                                         >  2:5318140/1‑101 (MQ=255)
                   gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                   <  1:6906728/101‑1 (MQ=255)
                        ccccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                              <  2:508273/99‑1 (MQ=255)
                        caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..GGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                              >  2:199076/1‑101 (MQ=255)
                        caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                              <  2:2586243/101‑1 (MQ=255)
                           cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                           >  1:3512865/1‑101 (MQ=255)
                            acGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                          <  1:2674158/101‑1 (MQ=255)
                              gCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                        >  1:3699672/1‑101 (MQ=255)
                               cAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                       <  2:6447937/101‑1 (MQ=255)
                                aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                      <  2:4800391/101‑1 (MQ=255)
                                    gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                  <  1:1275294/101‑1 (MQ=255)
                                       cTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                               >  2:421735/1‑101 (MQ=255)
                                         gCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                             >  1:2829019/1‑101 (MQ=255)
                                          caccacCGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                            <  2:6628025/101‑1 (MQ=255)
                                          aacaggatgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  2:7109926/93‑1 (MQ=2)
                                           ggccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA.CTGACCCTGCAA.C..GGCCTATGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  1:4917243/5‑101 (MQ=2)
                                            tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                           <  1:5206080/95‑1 (MQ=0)
                                            gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          <  1:4851079/96‑1 (MQ=0)
                                             cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                          >  1:6029075/6‑101 (MQ=0)
                                              ctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        >  1:2529757/5‑101 (MQ=2)
                                              cccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                        <  2:3558237/100‑1 (MQ=255)
                                               ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:4783138/101‑1 (MQ=255)
                                               ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                       <  1:1498088/101‑1 (MQ=255)
                                                 ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGACAcc                                                     <  1:5596740/101‑2 (MQ=2)
                                                  gtccccccccccGCGGGGGCGGGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                    <  1:199079/95‑1 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                     <  1:695704/101‑1 (MQ=2)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                    >  2:2399946/1‑101 (MQ=255)
                                                  gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                    <  2:6335244/101‑1 (MQ=255)
                                                  gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                     <  1:5501028/101‑1 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                    >  1:3236177/1‑100 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                    <  2:3642207/101‑2 (MQ=2)
                                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                                   >  2:1840611/1‑101 (MQ=255)
                                                    cccTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                   <  1:1009470/99‑3 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGATCGAAAcggggc                                                 >  1:1689402/1‑96 (MQ=2)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                  <  1:5818317/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcc                                                  <  1:5367178/101‑1 (MQ=255)
                                                    cGCTCCCCCCGCAGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                  >  1:5054845/1‑100 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcc                                                >  1:515150/1‑95 (MQ=0)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                 >  1:4453207/1‑99 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                 <  2:1009332/101‑3 (MQ=2)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                 >  1:2200983/1‑101 (MQ=255)
                                                     gCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                 >  2:6178962/1‑101 (MQ=255)
                                                      cTCCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                <  2:1496448/101‑4 (MQ=2)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                <  1:2126380/101‑6 (MQ=0)
                                                       tCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                >  1:4253675/1‑96 (MQ=0)
                                                       cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                               <  1:1476743/100‑5 (MQ=2)
                                                        ccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                               <  2:3767818/101‑7 (MQ=0)
                                                          ccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                            >  2:6048782/1‑94 (MQ=2)
                                                            ccGCGGGGGCGTGGATCGAAACACCAATCCCGAA.GC.CAACGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagccgcc                                           <  1:3339153/101‑11 (MQ=0)
                                                             cgcgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                         >  2:4496944/1‑101 (MQ=255)
                                                             cgcgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCgg                                         <  1:7115624/101‑1 (MQ=255)
                                                               cgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                       <  1:245524/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                      <  1:3012629/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                      <  1:1848843/101‑1 (MQ=255)
                                                                gggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGGGCGGCgg                                      >  1:4619449/1‑100 (MQ=255)
                                                                   ggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAc                                   >  1:5277101/1‑101 (MQ=255)
                                                                    gCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                  <  2:5670784/101‑1 (MQ=255)
                                                                     cGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                 >  1:1597288/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                 >  2:299062/1‑101 (MQ=255)
                                                                     cGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                 >  2:556503/1‑101 (MQ=255)
                                                                      gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                >  2:5833695/1‑101 (MQ=255)
                                                                      gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                >  2:3471904/1‑101 (MQ=255)
                                                                      gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                                >  2:834570/1‑100 (MQ=255)
                                                                        ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                              <  1:4028307/101‑1 (MQ=255)
                                                                        ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                              >  2:1298682/1‑101 (MQ=255)
                                                                        ggATCGAAACAAACCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcg                              >  1:6330173/1‑95 (MQ=255)
                                                                          aTCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                            <  2:3953684/101‑1 (MQ=255)
                                                                          aTCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                            <  2:2110527/101‑1 (MQ=255)
                                                                          aTCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGAcccgcccc                            >  1:4094448/1‑101 (MQ=255)
                                                                            cGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                          >  2:7048006/1‑101 (MQ=255)
                                                                             caaaCAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                         <  2:6931124/100‑1 (MQ=255)
                                                                              aaaCACCCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                        <  2:4142187/101‑1 (MQ=255)
                                                                               aacaacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                       >  2:2769265/1‑101 (MQ=255)
                                                                               aacaacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                       <  2:4905911/101‑1 (MQ=255)
                                                                                  aacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                    <  2:405012/101‑1 (MQ=255)
                                                                                    cccGACCATG.GC.C..CGCCCCCGCCCCCCGCT.GC.CGTCCCTCCCCCCGCGGGGGCGTGGATCGAAACATCCCCGGATCGGCGTGACCCGCCCCGCCCATCGGt                  <  1:2267905/101‑1 (MQ=25)
                                                                                       ccgtctgcgC.C..CGCCCCCCCGCCGCCCT.GA.CCTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                <  2:2274059/93‑1 (MQ=25)
                                                                                        atgacg.gC.C..CCCCTCAGCGCCGCGCT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt              <  2:5913933/96‑1 (MQ=37)
                                                                                         tCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGCTc             >  1:920977/1‑101 (MQ=255)
                                                                                         tCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc             >  1:3429573/1‑101 (MQ=255)
                                                                                         tCATG.CC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc             <  2:3088705/101‑1 (MQ=255)
                                                                                          cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc            >  1:6628816/1‑101 (MQ=255)
                                                                                          cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc            <  1:6436711/101‑1 (MQ=255)
                                                                                           agg.gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccc           <  2:1768122/99‑1 (MQ=255)
                                                                                               gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc        >  1:80670/1‑101 (MQ=255)
                                                                                               gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc        <  2:6825148/101‑1 (MQ=255)
                                                                                               gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc        <  2:1197048/101‑1 (MQ=255)
                                                                                                c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg       <  1:6187462/101‑1 (MQ=255)
                                                                                                c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcg       >  2:1647479/1‑101 (MQ=255)
                                                                                                        cTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg  <  1:5866089/101‑1 (MQ=255)
                                                                                                        cTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCgggg  >  1:6749905/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
GGGCGTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGG  >  NC_014034/1397450‑1397650

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG