Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,554 G→A 14.0% S479N (AGC→CAC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5540G→A14.0% 174.7 ‑2.1 ‑0.0511S479N (AGC→AAC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (30/42):  ref base (228/210):  total (258/253)
Fisher's exact test strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 548 total aligned reads displayed.

GTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397454‑1397653
                                                                                                         |                                                                                                     
    aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGcgcc                                                                                                   <  2:2081059/101‑1 (MQ=255)
    aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGcgcc                                                                                                   >  1:525587/1‑101 (MQ=255)
      tgAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGcgccgc                                                                                                 <  2:4346323/100‑1 (MQ=255)
      cGAAACACCGTCATCCCGACGCAACCGCCCTGCACCACCGGACGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CAGATCATG.GC.C..CGCCGCAGcgccgc                                                                                                 >  2:5671130/1‑101 (MQ=38)
      cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGcgccgc                                                                                                 >  1:3876433/1‑101 (MQ=255)
      cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGcgccgc                                                                                                 >  1:2572259/1‑101 (MQ=255)
       gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCcgcg                                                                                                <  1:947210/101‑1 (MQ=255)
          acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.g                                                                                            <  2:6381641/101‑1 (MQ=255)
              cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGt                                                                                       <  1:2470117/101‑1 (MQ=255)
               gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                      <  2:532015/101‑1 (MQ=255)
               gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                      <  1:6906728/101‑1 (MQ=255)
                tcatcaCCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCg                                                                                     >  1:7131550/1‑101 (MQ=255)
                  ccgatctcGCAACCGCCNTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                   <  2:1872467/94‑1 (MQ=255)
                    ccccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                 <  2:508273/99‑1 (MQ=255)
                    caccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                 <  2:583081/101‑1 (MQ=255)
                      ccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                               >  2:3012516/1‑101 (MQ=255)
                       cacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                              >  1:3512865/1‑101 (MQ=255)
                          gccgccGCCCGGCCCCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                           <  2:4212504/97‑1 (MQ=255)
                            aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCgg                                                                         <  2:4800391/101‑1 (MQ=255)
                            aaCCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCCCgg                                                                         >  1:2018806/1‑101 (MQ=255)
                                gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                     <  1:1705566/101‑1 (MQ=255)
                                gCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                     <  1:1275294/101‑1 (MQ=255)
                                  ccTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCgg                                                                   >  2:6526855/1‑100 (MQ=255)
                                    ggaacaggatgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGa                                                                <  2:6941236/91‑1 (MQ=2)
                                       ggccccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAA..CTGACCCTGCAA.C..GGCCTATGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  1:4917243/5‑101 (MQ=2)
                                       cccaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  2:4996078/100‑1 (MQ=255)
                                       accaccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  2:1546589/101‑1 (MQ=255)
                                        tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                              <  1:5206080/95‑1 (MQ=0)
                                        gcgctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:4851079/96‑1 (MQ=0)
                                        cgctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             >  1:6461535/7‑101 (MQ=2)
                                         cgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  1:6074509/96‑1 (MQ=0)
                                         cgagcgTCGCGCCCCCCGCGGGGGCGTGGATCGAACCACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GG.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                             <  2:3569228/96‑1 (MQ=2)
                                          gctggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           <  1:5557019/98‑1 (MQ=2)
                                          gagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                            <  2:6254673/97‑1 (MQ=2)
                                          ctgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                           >  2:235375/5‑101 (MQ=2)
                                           tgcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:3655360/98‑1 (MQ=2)
                                           ggcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  2:1716426/98‑1 (MQ=2)
                                           ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:4783138/101‑1 (MQ=255)
                                           ccGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                          <  1:117172/101‑1 (MQ=255)
                                            tggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         >  1:2881944/2‑101 (MQ=2)
                                            cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                         >  1:2161777/1‑101 (MQ=255)
                                             ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTG.ATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                        >  1:2292913/1‑100 (MQ=2)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGCGTAGATCTCG.GT.C..TGCGTGCGC.TCGCGGT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       <  2:1243245/101‑1 (MQ=2)
                                              gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                       >  1:1501046/1‑101 (MQ=255)
                                               gcGCGCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                       <  2:3266473/100‑2 (MQ=0)
                                                cccTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      <  1:1009470/99‑3 (MQ=2)
                                                cGCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                      <  1:6764595/101‑3 (MQ=2)
                                                cGCTCCCCCCGCAGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                                                     >  1:5054845/1‑100 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGGTCGAAAcggggcc                                                   >  1:515150/1‑95 (MQ=0)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAACATcga                                                    >  1:536615/1‑99 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                     >  2:1429501/1‑98 (MQ=2)
                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACaaaa                                                     >  2:2215614/1‑98 (MQ=2)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                    >  1:1944510/1‑97 (MQ=2)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                    <  2:247653/101‑5 (MQ=2)
                                                  cTCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                    <  1:1482934/101‑5 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                  <  1:1659988/101‑5 (MQ=2)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                   >  1:2760202/1‑96 (MQ=0)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                  <  1:5752945/101‑1 (MQ=255)
                                                   tCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                  >  1:4578019/1‑101 (MQ=255)
                                                    ccccccGCGGGGGCGTGGATCGAAACTAT.GCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                <  1:594999/101‑10 (MQ=2)
                                                    ccccccGCGGGGGCGTGGATCGAAACACC.AATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagc                                                  <  2:3767818/101‑7 (MQ=0)
                                                    ccccccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                 >  2:1789625/1‑101 (MQ=255)
                                                      ccccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatc                                               >  2:6048782/1‑94 (MQ=2)
                                                       cccGCGGGGGCGTGGATCGAAACACC.CCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                              <  2:725100/101‑9 (MQ=0)
                                                       cccGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                              <  2:6207149/101‑1 (MQ=255)
                                                        ccGCGGGGGCGTGGATCGAAACACC.AATCCCGAA.GC.CAACGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagccgcc                                              <  1:3339153/101‑11 (MQ=0)
                                                          gcgGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCGGATCgcg                                           >  2:2845732/1‑99 (MQ=255)
                                                            gggggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                         <  1:3012629/101‑1 (MQ=255)
                                                              gggCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTgc                                       >  1:4419965/1‑100 (MQ=255)
                                                                 cGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                    >  2:556503/1‑101 (MQ=255)
                                                                 cGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                    >  2:2668009/1‑101 (MQ=255)
                                                                  gTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                   >  2:3471904/1‑101 (MQ=255)
                                                                  gTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                                   >  2:834570/1‑100 (MQ=255)
                                                                    ggATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                 >  2:3637279/1‑101 (MQ=255)
                                                                    ggATCGAAACAAA.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcg                                 >  1:6330173/1‑95 (MQ=255)
                                                                       gcGAAACACC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                              <  1:3451388/100‑1 (MQ=255)
                                                                        cGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                             >  2:7048006/1‑101 (MQ=255)
                                                                         gAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                            >  1:4048100/1‑101 (MQ=255)
                                                                          aaaCACC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           <  2:4142187/101‑1 (MQ=255)
                                                                          aaaCAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           >  2:4462846/1‑101 (MQ=255)
                                                                          aaaCAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                           >  2:2128467/1‑101 (MQ=255)
                                                                           aacaac.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                          >  1:4170752/1‑101 (MQ=255)
                                                                             caac.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                        >  1:83178/1‑101 (MQ=255)
                                                                              aac.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                       <  2:405012/101‑1 (MQ=255)
                                                                               ac.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                      >  1:2216355/1‑101 (MQ=255)
                                                                                  ccGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                    <  1:3251595/101‑1 (MQ=255)
                                                                                     atgacg.gC.C..CCCCTCAGCGCCGCGCT.TG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                 <  2:5913933/96‑1 (MQ=37)
                                                                                     aTCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                 <  1:5841591/101‑1 (MQ=255)
                                                                                     aTCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                 <  1:3739877/101‑1 (MQ=255)
                                                                                      tCATG.CC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                <  2:3088705/101‑1 (MQ=255)
                                                                                       cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc               >  1:6628816/1‑101 (MQ=255)
                                                                                          g.gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc            >  1:7000504/1‑101 (MQ=255)
                                                                                          g.gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc            >  2:1024031/1‑101 (MQ=255)
                                                                                            gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcccccc           <  2:6825148/101‑1 (MQ=255)
                                                                                             c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGCTCCCCCcg          >  2:3937618/1‑101 (MQ=255)
                                                                                               c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc         <  2:4672494/101‑1 (MQ=255)
                                                                                                  cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg        >  1:5457524/1‑101 (MQ=255)
                                                                                                       cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc   >  1:6232546/1‑101 (MQ=255)
                                                                                                       cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc   <  2:6665579/101‑1 (MQ=255)
                                                                                                        aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg  >  2:2890767/1‑101 (MQ=255)
                                                                                                         |                                                                                                     
GTGGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAAC.CCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCG  >  NC_014034/1397454‑1397653

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG