Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,556 G→A 18.1% A480T (GCC→AGC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5560G→A18.1% 240.2 ‑1.6 ‑0.0497A480T (GCC→ACC) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (40/50):  ref base (212/192):  total (252/245)
Fisher's exact test strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 551 total aligned reads displayed.

GGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397456‑1397656
                                                                                                        |                                                                                                      
ggATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcg                                                                                                        <  2:6042473/101‑1 (MQ=255)
 gATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAgcgc                                                                                                       >  2:6249996/1‑101 (MQ=255)
  aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgcc                                                                                                      <  1:6639031/101‑1 (MQ=255)
  aTCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgcc                                                                                                      <  2:2081059/101‑1 (MQ=255)
   tCGAAACCCCGTCATCACCACGCAACCGCCCTGCTCCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccg                                                                                                     <  2:4583654/101‑1 (MQ=255)
    cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGCTCATGGC.C..CGCCTCAGcgccgc                                                                                                    >  1:3978364/1‑101 (MQ=255)
    cGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGcgccgc                                                                                                    <  2:3468031/101‑1 (MQ=255)
     gAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCcgcg                                                                                                   >  2:6779249/1‑101 (MQ=255)
      aaaCAACGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCgcgc                                                                                                  >  2:111864/1‑101 (MQ=255)
       aaCACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCt                                                                                                 >  1:4920649/1‑101 (MQ=255)
        acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.G..CGCCTCAGCGCCgcgcgg                                                                                                >  2:6918781/1‑99 (MQ=255)
        acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.g                                                                                               >  2:418906/1‑101 (MQ=255)
         ccccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                              <  1:537138/99‑1 (MQ=255)
            cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGt                                                                                          <  1:729345/101‑1 (MQ=255)
             gTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTc                                                                                         <  1:2607001/101‑1 (MQ=255)
               catcaCCACGCACCCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGc                                                                                       >  1:5078247/1‑101 (MQ=255)
                ccgatctcGCAACCGCCNTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                      <  2:1872467/94‑1 (MQ=255)
                atcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                      >  2:7099022/1‑101 (MQ=255)
                  caccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                    >  2:5515133/1‑101 (MQ=255)
                   cccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                                    <  1:5517534/100‑1 (MQ=255)
                    ccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                                  >  2:3012516/1‑101 (MQ=255)
                      acGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccccc                                                                                >  2:4324207/1‑101 (MQ=255)
                       cGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                               <  1:3259696/101‑1 (MQ=255)
                        gCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgc                                                                              <  2:407367/101‑1 (MQ=255)
                         cAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcgcg                                                                             <  2:6447937/101‑1 (MQ=255)
                              gCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                        <  1:1275294/101‑1 (MQ=255)
                              gCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                        >  2:5287233/1‑101 (MQ=255)
                                 cTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                     >  1:4074675/1‑101 (MQ=255)
                                    gcgcgctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCAGATC.C..CCAATCGGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:4864040/94‑1 (MQ=0)
                                    caccacCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:3896283/101‑1 (MQ=255)
                                    caccacCGGTCGCC.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                  <  2:6628025/101‑1 (MQ=255)
                                     accaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                 >  1:5224458/1‑101 (MQ=255)
                                      tcttgcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACTCCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  1:5848560/95‑1 (MQ=2)
                                      gcgctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                <  2:3552093/96‑1 (MQ=0)
                                      cgctgcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                >  1:6461535/7‑101 (MQ=2)
                                        gctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  1:6913257/4‑101 (MQ=2)
                                        gagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                               >  1:2802549/5‑101 (MQ=2)
                                        ctgcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                              >  2:235375/5‑101 (MQ=2)
                                         ggcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  2:1716426/98‑1 (MQ=2)
                                         ccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                             <  1:117172/101‑1 (MQ=255)
                                          cGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATGGATAc                                                            >  1:2165874/1‑101 (MQ=255)
                                          cGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                            <  2:148008/101‑1 (MQ=255)
                                           ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGACAcc                                                           <  1:5596740/101‑2 (MQ=2)
                                           ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                           >  1:2292913/1‑100 (MQ=2)
                                            gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                           >  1:6001396/1‑101 (MQ=2)
                                            gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          >  1:1501046/1‑101 (MQ=255)
                                            gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          <  1:3343300/101‑1 (MQ=255)
                                            gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                          <  2:6335244/101‑1 (MQ=255)
                                             gcGCG.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                          <  2:3266473/100‑2 (MQ=0)
                                              cccT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         <  1:1009470/99‑3 (MQ=2)
                                              cGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                         <  1:2225590/101‑3 (MQ=2)
                                               gCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                      <  2:5667150/101‑7 (MQ=2)
                                               gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                       <  1:6100179/101‑3 (MQ=2)
                                               gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcga                                                       >  1:4453207/1‑99 (MQ=2)
                                               gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACaaaa                                                        >  2:2215614/1‑98 (MQ=2)
                                               gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCTCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                       <  2:4782593/101‑1 (MQ=255)
                                               gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                       >  2:6178962/1‑101 (MQ=255)
                                                cT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                      <  2:5051721/101‑4 (MQ=2)
                                                cT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaa                                                       <  2:247653/101‑5 (MQ=2)
                                                 t.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                    <  2:4446717/101‑9 (MQ=2)
                                                 t.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     >  1:3533674/1‑97 (MQ=2)
                                                 t.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     <  1:1659988/101‑5 (MQ=2)
                                                  cccccccGCGGGGGCGTGGATCGAAACTATGCTGGCTCGGC.C..GAGTTCA.CGGCGGGCT.GT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                    <  1:3764016/100‑9 (MQ=2)
                                                  cccccccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatg                                                     <  1:1476743/100‑5 (MQ=2)
                                                   ccccccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCgg                                                    >  2:1789625/1‑101 (MQ=255)
                                                         gcgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCCGCGGATCgcg                                              >  2:2845732/1‑99 (MQ=255)
                                                          cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                             >  1:2918985/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAAATCCGCGGATCGGCGt                                            >  1:6765551/1‑101 (MQ=255)
                                                           gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAAATCCGCGGGGCGGCgg                                            >  1:4619449/1‑100 (MQ=255)
                                                            ggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTg                                           >  2:7013608/1‑101 (MQ=255)
                                                             gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                          >  2:1600725/1‑101 (MQ=255)
                                                                cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                       >  2:2668009/1‑101 (MQ=255)
                                                                 gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                      <  1:3675838/101‑1 (MQ=255)
                                                                 gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccc                                      >  2:834570/1‑100 (MQ=255)
                                                                  tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                     >  2:5783826/1‑101 (MQ=255)
                                                                   ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                    >  1:706735/1‑101 (MQ=255)
                                                                   ggATCGAAACAAACCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcg                                    >  1:6330173/1‑95 (MQ=255)
                                                                    gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                   >  2:3980630/1‑101 (MQ=255)
                                                                    gATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                   <  1:5807529/101‑1 (MQ=255)
                                                                     aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCGGCGCT.GA.CGGCGCTCCCCACGAGGGGGCGTGGAGCGAAACATCCGGGGAGCGCCGTGGccccccgc                                  >  1:1716492/1‑99 (MQ=255)
                                                                     aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                  >  1:2493794/1‑101 (MQ=255)
                                                                     aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                  <  1:4834343/101‑1 (MQ=255)
                                                                        gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                               >  1:4238431/1‑101 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                              >  2:2128467/1‑101 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                              <  1:1979200/101‑1 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                              >  2:5881249/1‑101 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                              <  1:3098351/101‑1 (MQ=255)
                                                                         aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                              >  1:3471473/1‑101 (MQ=255)
                                                                            caacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                           <  1:973136/101‑1 (MQ=255)
                                                                             accccGATCATGCC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                          <  2:1266365/99‑1 (MQ=255)
                                                                              acCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                         >  1:529826/1‑101 (MQ=255)
                                                                               cccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCggc                        >  1:4862854/1‑100 (MQ=255)
                                                                                    tCATGCC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                   <  2:3088705/101‑1 (MQ=255)
                                                                                                gCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGCTCCCCCCGCgg          >  1:88813/1‑101 (MQ=255)
                                                                                                 ccTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggg         >  2:3740300/1‑101 (MQ=255)
                                                                                                   tCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggggg       >  2:3608900/1‑101 (MQ=255)
                                                                                                    cggcgcCGCGCG.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc      <  1:208297/99‑1 (MQ=255)
                                                                                                     aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg     >  2:2890767/1‑101 (MQ=255)
                                                                                                       cgccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTg   <  1:6250315/101‑1 (MQ=255)
                                                                                                        gccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTgg  >  1:5190706/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
GGATCGAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGG  >  NC_014034/1397456‑1397656

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG