Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,561 G→T 9.6% A481A (GCG→GAT RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5610G→T 9.6% 95.5 ‑1.8 ‑0.0498A481A (GCG→GCTRCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/29):  ref base (227/222):  total (247/251)
Fisher's exact test strand distribution p-value = 1.72e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.98e-01

Only 100 of 544 total aligned reads displayed.

GAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397461‑1397661
                                                                                                        |                                                                                                      
 aaaCAACGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCgcgc                                                                                                       >  2:111864/1‑101 (MQ=255)
   acacCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.G..CGCCTCAGCGCCgcgcgg                                                                                                     >  2:6918781/1‑99 (MQ=255)
    ccccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                                   <  1:537138/99‑1 (MQ=255)
      ccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.Cg                                                                                                <  1:3521091/101‑1 (MQ=255)
          catcaCCACGCACCCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGc                                                                                            >  1:5078247/1‑101 (MQ=255)
           atcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                           >  2:7099022/1‑101 (MQ=255)
           atcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                           <  1:2921706/101‑1 (MQ=255)
            tcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTc                                                                                          >  2:378529/1‑101 (MQ=255)
             caccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                         >  1:4920225/1‑101 (MQ=255)
             caccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                         <  2:583081/101‑1 (MQ=255)
              cccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                                         <  1:5517534/100‑1 (MQ=255)
              accacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccc                                                                                        <  1:2384411/101‑1 (MQ=255)
               ccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                                       <  1:1491611/101‑1 (MQ=255)
                  cGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCtanatcgg                                                                                    >  1:1872539/1‑94 (MQ=255)
                  cGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                                    <  2:40629/101‑1 (MQ=255)
                   gCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.A..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCGCCCcgc                                                                                   >  1:3853190/1‑101 (MQ=255)
                         gCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGc                                                                             >  2:5506357/1‑101 (MQ=255)
                           ccTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCgg                                                                           >  2:6526855/1‑100 (MQ=255)
                             tGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:4160696/101‑1 (MQ=255)
                               gcgcgctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCAGATC.C..CCAATCGGCCGGGCTCG.AT.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:4864040/94‑1 (MQ=0)
                                cccaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:1234243/100‑1 (MQ=255)
                                atcgagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  1:6087492/94‑1 (MQ=0)
                                accaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  1:2110607/1‑101 (MQ=255)
                                 tcgagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  2:6387057/7‑101 (MQ=0)
                                  cgagcgTCGCG.CCCCCCGCGGGGGCGTGGATCGAACCACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GG.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  2:3569228/96‑1 (MQ=2)
                                  aggatgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  1:4604102/96‑1 (MQ=2)
                                   gagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    >  1:2802549/5‑101 (MQ=2)
                                   accGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  1:521257/1‑101 (MQ=255)
                                   accGGTCGCT.CCCCCCGCGGGGGCGTGGACCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCAGCGCT.GA.CGTCGCTCCCCCCGCGGGCGAGTGGATCGaa                                                                   >  2:4743511/1‑101 (MQ=37)
                                    tgcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:3655360/98‑1 (MQ=2)
                                    tgcgTCGCT..CCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:7002107/98‑1 (MQ=2)
                                    ccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:4783138/101‑1 (MQ=255)
                                    ccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:117172/101‑1 (MQ=255)
                                     gcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:1604138/99‑1 (MQ=2)
                                      ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.GAT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGACAcc                                                                <  1:5596740/101‑2 (MQ=2)
                                      cgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  1:6919218/100‑1 (MQ=2)
                                       gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                               <  2:5006863/101‑1 (MQ=255)
                                        tCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                               <  2:3642207/101‑2 (MQ=2)
                                        gtccccccccccGCGGGGGCGGGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                               <  1:199079/95‑1 (MQ=255)
                                         cccT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                              <  1:1009470/99‑3 (MQ=2)
                                         cGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGATCGAAAcggggc                                                            >  1:1689402/1‑96 (MQ=2)
                                          gCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcg                                                           <  2:5667150/101‑7 (MQ=2)
                                          gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                             <  1:6403160/101‑4 (MQ=2)
                                           cT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcga                                                          <  2:4268808/101‑8 (MQ=2)
                                           cT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgat                                                           <  2:1496448/101‑4 (MQ=2)
                                            t.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGCGGGTCCAAAcggggcgga                                                         >  1:956589/1‑93 (MQ=0)
                                             cccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaa                                                         <  2:6072883/100‑9 (MQ=2)
                                              ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGgaaaggggcgaag                                                        >  2:4411293/1‑91 (MQ=2)
                                               cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TCGA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                                       <  2:1262758/101‑11 (MQ=0)
                                               cccccGCGGGGGCGTGGATCGAAACACCAATGCCGAAGC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaagcc                                                         <  1:3061395/101‑8 (MQ=0)
                                                 cccGCGGGGGCGTGGATCGAAACACCCCGAACCTGTCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                      <  2:2045058/101‑9 (MQ=0)
                                                 cccGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATc                                                      <  2:6207149/101‑1 (MQ=255)
                                                     cgGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                  >  2:3101736/1‑101 (MQ=255)
                                                      gggggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGAAAAATCCGCGGATCGGCGt                                                 >  1:6765551/1‑101 (MQ=255)
                                                        gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTgc                                               >  1:4419965/1‑100 (MQ=255)
                                                        gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                               >  2:1600725/1‑101 (MQ=255)
                                                        gggCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                               >  2:1863202/1‑101 (MQ=255)
                                                          gCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcc                                             <  1:2520439/101‑1 (MQ=255)
                                                           cGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAccc                                            >  2:299062/1‑101 (MQ=255)
                                                            gTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                           <  1:5846992/101‑1 (MQ=255)
                                                             tGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAGCGGCGTGCcccgc                                          >  2:829652/1‑101 (MQ=255)
                                                              ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                         <  1:5978329/101‑1 (MQ=255)
                                                              ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                         >  1:6665921/1‑101 (MQ=255)
                                                              ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                         >  1:706735/1‑101 (MQ=255)
                                                              ggATCGAAACAACCCGATCATGGC.C..CGCCTCAGAGCCGCGCT.GA.CGTGGCTCCCCCCGCGGGGGCGTGGATCGAAAAATACGCGGATCGGGGTGAccagcc                                         >  2:3294105/1‑101 (MQ=37)
                                                              ggATCGAAACAACCCGATCAAGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGTTCGAAACATCCGCCGATCCGCGGTAcccgcc                                         >  2:2204850/1‑101 (MQ=37)
                                                              ggATCGAAACAAACCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGaagatcg                                         >  1:6330173/1‑95 (MQ=255)
                                                               gATGGAAGCAGCCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                        <  2:1784131/101‑1 (MQ=255)
                                                                aTCGAAACACCCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                       <  2:2449566/101‑1 (MQ=255)
                                                                aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                       <  2:3953684/101‑1 (MQ=255)
                                                                aTCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGCGAcccgcccc                                       >  1:4094448/1‑101 (MQ=255)
                                                                 tCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTGCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                      <  1:5166381/101‑1 (MQ=255)
                                                                 gcGAAACACCCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                      <  1:3451388/100‑1 (MQ=255)
                                                                  cGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                                     >  2:6325008/1‑101 (MQ=255)
                                                                  cGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAccccggccgc                                     >  1:2260225/1‑94 (MQ=255)
                                                                   gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                    >  1:4238431/1‑101 (MQ=255)
                                                                   gAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                    <  1:334024/101‑1 (MQ=255)
                                                                    aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                   >  2:2128467/1‑101 (MQ=255)
                                                                    aaaCAACCCGATCATGGC.C..CGCCTCAGCGCCGCCCT.GA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGGAACATCCGCGGCGCGGGGGGACCcccccccccc                                   >  2:2305795/1‑101 (MQ=25)
                                                                        aacCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                               <  2:2667640/101‑1 (MQ=255)
                                                                         acCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                              >  1:529826/1‑101 (MQ=255)
                                                                           ccGATCATGGC.C..CGCCTCCGCCCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                            <  2:5224193/101‑1 (MQ=255)
                                                                           ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                            <  1:775151/101‑1 (MQ=255)
                                                                           ccGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                            <  1:3251595/101‑1 (MQ=255)
                                                                              aTCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCt                         <  1:3739877/101‑1 (MQ=255)
                                                                               tCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                        >  2:5895162/1‑101 (MQ=255)
                                                                                cATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                       <  1:146848/101‑1 (MQ=255)
                                                                                   ggC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc                    >  2:1723724/1‑101 (MQ=255)
                                                                                       c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgc                 <  1:4856050/101‑1 (MQ=255)
                                                                                          cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCcgcg                >  2:2837102/1‑101 (MQ=255)
                                                                                            ccTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCggg              >  2:3495163/1‑101 (MQ=255)
                                                                                               cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc           >  1:6232546/1‑101 (MQ=255)
                                                                                               cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc           <  2:6665579/101‑1 (MQ=255)
                                                                                                aGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCg          >  2:2890767/1‑101 (MQ=255)
                                                                                                  cgccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTg        <  1:2189149/101‑1 (MQ=255)
                                                                                                    ccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa      <  1:2673753/101‑1 (MQ=255)
                                                                                                     cgcgCT.GA.CGTCGCCCCCCCCGCGGGGGCGNGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGAt     <  1:861846/101‑1 (MQ=255)
                                                                                                      gcgcG.GA.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc    <  2:6774689/101‑1 (MQ=255)
                                                                                                       cgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCg   <  1:5789909/101‑1 (MQ=255)
                                                                                                        gcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa  >  2:4620988/1‑101 (MQ=255)
                                                                                                        |                                                                                                      
GAAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATGGC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397461‑1397661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG