Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,562 C→A 9.4% M482M (CTG→ACC)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,5620C→A 9.4% 99.6 ‑1.0 0.0496M482M (CTG→ATG) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (20/27):  ref base (225/223):  total (245/251)
Fisher's exact test strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 538 total aligned reads displayed.

AAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397462‑1397661
                                                                                                         |                                                                                                     
aaaCAACGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCgcgc                                                                                                       >  2:111864/1‑101 (MQ=255)
   ccccGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.Ga                                                                                                   <  1:537138/99‑1 (MQ=255)
      cGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGt                                                                                               <  1:729345/101‑1 (MQ=255)
          atcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCt                                                                                           >  2:7099022/1‑101 (MQ=255)
           tcaCCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTc                                                                                          >  2:378529/1‑101 (MQ=255)
            caccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcc                                                                                         <  2:2586243/101‑1 (MQ=255)
             cccacGCAACCGCCCTGCACCACCGGTCGCTCCCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGCCGCTcc                                                                                         <  1:5517534/100‑1 (MQ=255)
              ccacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTcccc                                                                                       <  1:1491611/101‑1 (MQ=255)
               cacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                                      >  1:3512865/1‑101 (MQ=255)
               cacGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTccccc                                                                                      <  2:5362008/101‑1 (MQ=255)
                 cGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCcg                                                                                    <  1:2666845/101‑1 (MQ=255)
                  gCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.A..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCGCCCcgc                                                                                   >  1:3853190/1‑101 (MQ=255)
                           cTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTg                                                                          >  1:4074675/1‑101 (MQ=255)
                            tGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTgg                                                                         <  2:4160696/101‑1 (MQ=255)
                              caccacCGGTCGCC.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                       <  2:6628025/101‑1 (MQ=255)
                              aacaggatgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGGGGATc                                                                      >  2:5183325/9‑101 (MQ=0)
                               ggccccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAA.CTGACCCTGCAA.C..GGCCTATGCGCTG.GAC.GT.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     >  1:4917243/5‑101 (MQ=2)
                               cccaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      <  1:1234243/100‑1 (MQ=255)
                               accaccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                      >  1:2110607/1‑101 (MQ=255)
                                gcgctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:4851079/96‑1 (MQ=0)
                                 cgctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:4573649/97‑1 (MQ=0)
                                 cgagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                     <  1:6074509/96‑1 (MQ=0)
                                  gctggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  1:6913257/4‑101 (MQ=2)
                                  gagcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                    <  2:6254673/97‑1 (MQ=2)
                                  cccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   <  2:3558237/100‑1 (MQ=255)
                                  accGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                   >  1:521257/1‑101 (MQ=255)
                                   tgcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:3655360/98‑1 (MQ=2)
                                   ggcgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  2:1716426/98‑1 (MQ=2)
                                   ccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:4783138/101‑1 (MQ=255)
                                   ccGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                  <  1:117172/101‑1 (MQ=255)
                                    cGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:148008/101‑1 (MQ=255)
                                    cGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 >  2:6187143/1‑101 (MQ=255)
                                    cGGTCGCG.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                 <  2:4857397/101‑1 (MQ=255)
                                     tgTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGTGGGTCGGAAcgg                                                               >  1:5770183/2‑99 (MQ=0)
                                     ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACTTGATGACCA.G.AT.C..CGCAATCG.GCCGGGCTCGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGACAcc                                                                <  1:5596740/101‑2 (MQ=2)
                                     ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                >  2:6901987/1‑101 (MQ=255)
                                     ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                                                <  2:6990872/101‑1 (MQ=255)
                                     ggTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGCGGGCGTGGATCGACACa                                                                >  1:5160161/1‑101 (MQ=255)
                                      gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.AG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                               >  1:6306830/1‑101 (MQ=2)
                                      gTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                               <  1:2790491/101‑1 (MQ=255)
                                       gtccccccccccGCGGGGGCGGGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                                               <  1:199079/95‑1 (MQ=255)
                                       gcGCG.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaa                                                               <  2:3266473/100‑2 (MQ=0)
                                        cccT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                              <  1:1009470/99‑3 (MQ=2)
                                        cGCT.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCCCGGGGGCGGGGATCGAAAcggggc                                                            >  1:1689402/1‑96 (MQ=2)
                                        cGCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaa                                                              <  1:6764595/101‑3 (MQ=2)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                             >  2:2879311/1‑98 (MQ=2)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaa                                                             <  1:6403160/101‑4 (MQ=2)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                            >  2:6365235/1‑101 (MQ=255)
                                         gCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcg                                                            >  2:4998755/1‑101 (MQ=255)
                                           t.CCCCCCGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGCGGGTCCAAAcggggcgga                                                         >  1:956589/1‑93 (MQ=0)
                                           t.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCcgcg                                                          >  1:4578019/1‑101 (MQ=255)
                                            cccccccGCGGGGGCGTGGATCGAAACACCAATGCCGAA.GC.CAAGGCCGCAGCAGCG.GCT.GC.GGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACaaaaag                                                           <  2:2007422/100‑6 (MQ=0)
                                             ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGGTCGgaaaggggcgaag                                                        >  2:4411293/1‑91 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                        <  2:3622590/101‑10 (MQ=2)
                                             ccccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaag                                                        <  1:594999/101‑10 (MQ=2)
                                              cccccGCGGGGGCGTGGATCGAAACTATGCTGGC.TC.GA.C..CGAGTTCACGGCGGGCT.AT.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcgggtcgaaga                                                       <  2:1262758/101‑11 (MQ=0)
                                              cccccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgat                                                        <  1:2172133/101‑7 (MQ=2)
                                                cccGCGGGGGCGTGGATCGAAACACCCCGAACCTG.TCGG..CGGC.GAACACCGATAC.CG.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgatgatca                                                      <  2:6014010/101‑9 (MQ=0)
                                                 ccGCGGGGGCGTGGATCGAAACAACCCGATCATA.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCg                                                     >  1:3711377/1‑101 (MQ=255)
                                                    cgGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCg                                                  >  1:2918985/1‑101 (MQ=255)
                                                     gggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                 <  1:3012629/101‑1 (MQ=255)
                                                     gggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGt                                                 <  1:1848843/101‑1 (MQ=255)
                                                      ggggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCGCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCTTGGATCGAAAAATCCTCGGATCGGCGTg                                                <  1:5068423/101‑1 (MQ=38)
                                                       gggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTgc                                               >  1:4419965/1‑100 (MQ=255)
                                                       gggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                               >  2:1600725/1‑101 (MQ=255)
                                                       gggCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGa                                               >  2:1863202/1‑101 (MQ=255)
                                                           gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                           >  2:3471904/1‑101 (MQ=255)
                                                           gTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccg                                           <  1:5846992/101‑1 (MQ=255)
                                                            tGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                          >  2:5783826/1‑101 (MQ=255)
                                                            tGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgc                                          >  2:4223383/1‑101 (MQ=255)
                                                            tGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGAGCGGCGTGCcccgc                                          >  2:829652/1‑101 (MQ=255)
                                                             ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcc                                         <  2:4727461/101‑1 (MQ=255)
                                                             ggATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAcccgcc                                         >  2:561267/1‑101 (MQ=255)
                                                              gATGGAAGCAGCCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccc                                        <  2:1784131/101‑1 (MQ=255)
                                                               aTCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                       <  1:2360448/101‑1 (MQ=255)
                                                               aTCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgcccc                                       >  1:2493794/1‑101 (MQ=255)
                                                                gcGAAACACCCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccg                                      <  1:3451388/100‑1 (MQ=255)
                                                                 cGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGAcccgccccgc                                     >  2:2955546/1‑101 (MQ=255)
                                                                 cGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGGGAccccggccgc                                     >  1:2260225/1‑94 (MQ=255)
                                                                  gAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACccgccccgcc                                    <  1:334024/101‑1 (MQ=255)
                                                                   aaaCAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                   >  2:5881249/1‑101 (MQ=255)
                                                                   aaaCAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCcgccccgccc                                   >  2:2128467/1‑101 (MQ=255)
                                                                    aacaacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCa                                  >  2:2769265/1‑101 (MQ=255)
                                                                      caacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATc                                >  1:83178/1‑101 (MQ=255)
                                                                       aacCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCg                               <  2:405012/101‑1 (MQ=255)
                                                                        acCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCgg                              >  1:529826/1‑101 (MQ=255)
                                                                          ccGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTc                            <  1:3251595/101‑1 (MQ=255)
                                                                            ccgtctgcgC.C..CGCCCCCCCGCCGCCCT.GA.CCTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCg                           <  2:2274059/93‑1 (MQ=25)
                                                                              tCATG.CC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTc                        <  2:3088705/101‑1 (MQ=255)
                                                                               cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                       >  1:6628816/1‑101 (MQ=255)
                                                                               cATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTcc                       <  1:146848/101‑1 (MQ=255)
                                                                                  g.gC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTccccc                    >  1:7000504/1‑101 (MQ=255)
                                                                                     c.c..cGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCCTCGGTCGCTCCCCCcg                  >  2:3937618/1‑101 (MQ=255)
                                                                                               cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGc           >  1:6232546/1‑101 (MQ=255)
                                                                                               cAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCCGGGGc           >  2:2437122/1‑101 (MQ=255)
                                                                                                    ccgcGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGa      <  1:4233786/101‑1 (MQ=255)
                                                                                                      gcgcT.GC.CGTCGCGCCCCCGGGGGGGGCGGGGATGGAAACAGCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc    <  2:6765197/101‑1 (MQ=37)
                                                                                                      gcgcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATc    <  1:1600786/101‑1 (MQ=255)
                                                                                                        gcT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  2:6505999/101‑1 (MQ=255)
                                                                                                        gcT.GA.CGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa  <  2:2200888/101‑1 (MQ=255)
                                                                                                         |                                                                                                     
AAACACCGTCATCACCACGCAACCGCCCTGCACCACCGGTCGCT.CCCCCCGCGGGGGCGTGGATCGAAACAACCCGATCATG.GC.C..CGCCTCAGCGCCGCGCT.GA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGA  >  NC_014034/1397462‑1397661

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG