Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,872 C→T 1.8% intergenic (+176/+1722) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,8720C→T 1.8% 8.7 0.0 ‑0.8395intergenic (+176/+1722)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (3/4):  ref base (177/211):  total (180/215)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.76e-01

Only 100 of 422 total aligned reads displayed.

CCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGT  >  NC_014034/1397772‑1397972
                                                                                                     |                                                                                                    
ccccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAA.CCGGc                                                                                                      >  2:2942528/1‑101 (MQ=255)
 cccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCa                                                                                                     <  2:536603/101‑1 (MQ=255)
  ccccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAc                                                                                                    >  2:2241870/1‑101 (MQ=255)
    ccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCt                                                                                                  >  2:3593349/1‑101 (MQ=255)
    ccGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCt                                                                                                  <  2:1297394/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTc                                                                                                 <  2:4282501/101‑1 (MQ=255)
     cgcgGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTc                                                                                                 <  1:3649970/101‑1 (MQ=255)
         ggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGcc                                                                                             <  2:5943348/101‑1 (MQ=255)
         ggggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGcc                                                                                             <  1:6912260/101‑1 (MQ=255)
           ggCGTGGATCGACACGGTGTGCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGcccc                                                                                           >  1:6384997/1‑101 (MQ=255)
           ggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGcccc                                                                                           <  2:6144002/101‑1 (MQ=255)
           ggCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGcccc                                                                                           >  1:1566108/1‑101 (MQ=255)
            gCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccg                                                                                          <  1:5079196/101‑1 (MQ=255)
             cGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCCGCcccgc                                                                                         >  2:66757/1‑101 (MQ=255)
              gTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgcc                                                                                        >  1:7130141/1‑101 (MQ=255)
               tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgccc                                                                                       <  1:2180828/101‑1 (MQ=255)
               tGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgccc                                                                                       >  1:6724697/1‑101 (MQ=255)
               gggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgccc                                                                                       <  2:397122/100‑1 (MQ=255)
                ggATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCcccgcccg                                                                                      <  2:260623/101‑1 (MQ=255)
                 gATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCccgcccgc                                                                                     >  2:2346610/1‑101 (MQ=255)
                  aTCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCt                                                                                    <  1:5553563/101‑1 (MQ=255)
                    cgcaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCACCCCCCGCGGGGGCGTGGATCGAACC.CCGGCACCTCAGCCCCGCCCGCTGt                                                                                  <  2:7115410/98‑1 (MQ=255)
                    cGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGt                                                                                  >  2:1948290/1‑101 (MQ=255)
                     gAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGtc                                                                                 <  2:6035955/101‑1 (MQ=255)
                      aaaCGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGtct                                                                                >  2:301385/1‑101 (MQ=255)
                      aaaCGGTGTCCAGCGTCACATGCAGAGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGTGGCGTGGATGGAAAG.CCGGCCCCTCAGCCCCGCCCGCTggcc                                                                                >  2:838664/1‑98 (MQ=38)
                         cGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCAc                                                                             <  1:1300429/101‑1 (MQ=255)
                          ggTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCAcc                                                                            <  1:975120/101‑1 (MQ=255)
                           gtgtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCa                                                                           <  1:4300142/101‑1 (MQ=255)
                             gtCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAAc                                                                         >  2:4948209/1‑101 (MQ=255)
                               ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                       <  1:6105205/101‑1 (MQ=255)
                               ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                       >  1:5327976/1‑101 (MQ=255)
                                cAGCGTTACATGCAGGGGCCTGGCATCGCTATGTCGCCCCCTCCGCGGGGGCGTGGATCGAACC.CCGTCGCGTGAGCCCCGCCCGCTGTCTCACCAACAGt                                                                      <  2:5768059/101‑1 (MQ=25)
                                cAGCGTCACATGCAGCGGCGTCGCATCGCCATGTTGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGt                                                                      >  1:4588263/1‑101 (MQ=255)
                                   cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGACAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                   <  2:3449451/101‑1 (MQ=255)
                                   cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                   >  2:6530898/1‑101 (MQ=255)
                                    gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCCACAGTCGCt                                                                  >  2:5030005/1‑101 (MQ=255)
                                     tCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTc                                                                 <  1:1962609/101‑1 (MQ=255)
                                        caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                              >  2:4488143/1‑101 (MQ=255)
                                         aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                             >  1:3443096/1‑101 (MQ=255)
                                         aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                             <  2:6237216/101‑1 (MQ=255)
                                         aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                             >  1:2636965/1‑101 (MQ=255)
                                          tGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccccc                                                            <  2:3710678/101‑1 (MQ=255)
                                           gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                           <  1:3228553/101‑1 (MQ=255)
                                            cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                          >  1:5787870/1‑101 (MQ=255)
                                            cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                          <  2:2403050/101‑1 (MQ=255)
                                                ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                      <  1:1720056/101‑1 (MQ=255)
                                                ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                      >  1:4404867/1‑101 (MQ=255)
                                                  cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                    <  2:76373/101‑1 (MQ=255)
                                                    tCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                  <  1:3139060/101‑1 (MQ=255)
                                                    tCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                  <  1:4084601/101‑1 (MQ=255)
                                                     cGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTg                                                 <  1:5827616/101‑1 (MQ=255)
                                                      gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                >  1:6248666/1‑101 (MQ=255)
                                                      gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                >  1:3058421/1‑101 (MQ=255)
                                                      gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                <  2:1408418/101‑1 (MQ=255)
                                                      gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:5463396/1‑101 (MQ=255)
                                                       ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:3645993/10‑101 (MQ=0)
                                                       ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                >  2:89410/10‑101 (MQ=0)
                                                       cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                               >  2:6809085/1‑101 (MQ=255)
                                                        aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGac                                              >  1:4342214/1‑100 (MQ=255)
                                                        aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                              >  1:6529170/1‑101 (MQ=255)
                                                        aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                              <  2:1662495/101‑1 (MQ=255)
                                                         tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             >  1:5511151/1‑101 (MQ=255)
                                                         tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  1:180715/101‑1 (MQ=255)
                                                         tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                             <  2:2114615/101‑1 (MQ=255)
                                                          tcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTGGGTc                                             >  2:2001591/7‑101 (MQ=0)
                                                             cATGTCGCTCCCCCCGCGGGGGCGTGGATCGACAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                         <  2:5263506/101‑1 (MQ=255)
                                                             cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAACTCAGCCCCGCCCTCTGACTAACCCACAGCCGCTACCCACGCGGGGGCGGggctagagg                                         >  2:3338665/1‑94 (MQ=18)
                                                              aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  2:5562051/1‑101 (MQ=255)
                                                              aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        >  1:1895906/1‑101 (MQ=255)
                                                              aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                        <  2:4318636/101‑1 (MQ=255)
                                                               tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                       <  1:5456716/101‑1 (MQ=255)
                                                                  cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCNCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                    >  1:228197/1‑101 (MQ=255)
                                                                   gCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTAt                                   <  2:1566048/101‑1 (MQ=255)
                                                                      ccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                >  1:41830/1‑101 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACTCCATgggag                              >  2:4782182/1‑101 (MQ=255)
                                                                        ccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggag                              <  2:6384673/101‑1 (MQ=255)
                                                                           cgcgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggct                           <  1:6969335/101‑1 (MQ=255)
                                                                               ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                       >  1:4914886/1‑101 (MQ=255)
                                                                               ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                       >  1:3899701/1‑101 (MQ=255)
                                                                                    gTGGATCGAAAC.CCGGCACCTCAGCCCCGCNCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggct                  <  1:2442510/101‑1 (MQ=255)
                                                                                      ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                <  1:6048331/101‑1 (MQ=255)
                                                                                      ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                >  2:4715260/1‑101 (MQ=255)
                                                                                      ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                >  2:3168629/1‑101 (MQ=255)
                                                                                      ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                >  2:1826299/1‑101 (MQ=255)
                                                                                         tCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa             >  1:3572205/1‑101 (MQ=255)
                                                                                          cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc            >  1:2532651/1‑101 (MQ=255)
                                                                                           gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc           >  1:946481/1‑101 (MQ=255)
                                                                                           gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc           <  2:2515428/101‑1 (MQ=255)
                                                                                           gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc           >  1:4018941/1‑101 (MQ=255)
                                                                                           gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc           <  1:2931458/101‑1 (MQ=255)
                                                                                            aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt          <  2:41829/101‑1 (MQ=255)
                                                                                            aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt          >  2:1111249/1‑101 (MQ=255)
                                                                                             aaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCtt         <  1:33593/101‑1 (MQ=255)
                                                                                              aC.CCGGCAGCGCCGCACCGCCCGCTGCCTCACCACCAGTCGCCCCCCCCGCGGGGGTGTGGATCGAACCTCTATGGGAGGCTAGGGAGGCTACGCACCTTc        <  2:4373995/101‑1 (MQ=255)
                                                                                               c.ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCa       <  1:1252056/101‑1 (MQ=255)
                                                                                                 ccGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaa      <  1:1111284/101‑1 (MQ=255)
                                                                                                  cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa     <  1:4468561/101‑1 (MQ=255)
                                                                                                  cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa     <  1:3703205/101‑1 (MQ=255)
                                                                                                    gCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg   <  2:276406/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CCCCCCGCGGGGGCGTGGATCGAAACGGTGTCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGT  >  NC_014034/1397772‑1397972

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG