BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,397,902	A→G	1.9%	intergenic (+206/+1692)	RCAP_rcc01307 → / ← RCAP_rcc01309	hypothetical protein/IS66 family transposase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,397,902	0	A→G	1.9%	5.7 0.0 ‑0.1	364	intergenic (+206/+1692)	RCAP_rcc01307/RCAP_rcc01309	hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand): new base (3/4): ref base (158/199): total (161/203)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.72e-01

Only 100 of 392 total aligned reads displayed.


TCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397802‑1398002


tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                      <  2:392/101‑1 (MQ=255)


tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                      >  1:5262779/1‑101 (MQ=255)


tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                      >  1:174986/1‑101 (MQ=255)


tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                      <  1:193313/101‑1 (MQ=255)


tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                      >  1:5391031/1‑101 (MQ=255)


tCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACa                                                                                                      >  1:2528167/1‑101 (MQ=255)


 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                                                     <  1:6070077/101‑1 (MQ=255)


 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGGCTCACCAACAg                                                                                                     >  2:5456444/1‑101 (MQ=255)


 ccAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCCGCACCTCAGCCCCGCCCGCTGTCTCACCAACAg                                                                                                     >  2:1008322/1‑101 (MQ=255)


  cAGCGTTACATGCAGGGGCCTGGCATCGCTATGTCGCCCCCTCCGCGGGGGCGTGGATCGAACC.CCGTCGCGTGAGCCCCGCCCGCTGTCTCACCAACAGt                                                                                                    <  2:5768059/101‑1 (MQ=25)


   ggCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                                   <  2:2251611/100‑1 (MQ=255)


   aGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTc                                                                                                   >  2:5994689/1‑101 (MQ=255)


     cGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGc                                                                                                 <  1:2979159/101‑1 (MQ=255)


      gTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAgagatc                                                                                                >  1:6669260/1‑96 (MQ=255)


          caTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTcccc                                                                                            >  2:4488143/1‑101 (MQ=255)


           aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                                           >  1:2636965/1‑101 (MQ=255)


           aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                                           <  2:2677669/101‑1 (MQ=255)


           aTGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTccccc                                                                                           <  2:6237216/101‑1 (MQ=255)


             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGTGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                         <  1:6414849/101‑1 (MQ=255)


             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                         <  1:3228553/101‑1 (MQ=255)


             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                         <  1:845735/101‑1 (MQ=255)


             gCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcg                                                                                         >  2:4959450/1‑101 (MQ=255)


              cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                                        >  1:5787870/1‑101 (MQ=255)


              cAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgc                                                                                        <  2:2403050/101‑1 (MQ=255)


               aGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCcgcg                                                                                       >  1:4129411/1‑101 (MQ=255)


                  ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                                    >  2:3703056/1‑101 (MQ=255)


                  ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                                    >  1:4404867/1‑101 (MQ=255)


                  ggcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCgggg                                                                                    <  2:2363553/101‑1 (MQ=255)


                   gcgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGAACGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCggggg                                                                                   <  2:1200586/101‑1 (MQ=255)


                    cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                                                  <  1:3991848/101‑1 (MQ=255)


                    cgTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGc                                                                                  >  2:1561574/1‑101 (MQ=255)


                     gTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCg                                                                                 >  2:18976/1‑101 (MQ=255)


                      tCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                                                <  2:486133/101‑1 (MQ=255)


                      tCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGt                                                                                <  1:3139060/101‑1 (MQ=255)


                        gCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTgg                                                                              >  1:3058421/1‑101 (MQ=255)


                         ccatcgagcgTCTCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  2:725774/92‑1 (MQ=0)


                         ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                              >  2:89410/10‑101 (MQ=0)


                         ccatcgagcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCAATGCCGAAGCCAAGGCCGCAGCAGCGGCTGCGGTCGCTCCCCCCGCGGGGGCGTgg                                                                              <  2:803243/92‑1 (MQ=0)


                         cATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGa                                                                             >  2:1819357/1‑101 (MQ=255)


                          aTCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                            >  1:6529170/1‑101 (MQ=255)


                           tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCTGCGGGGGCGCGGATc                                                                           <  1:6866421/101‑1 (MQ=255)


                           tCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                           <  2:2114615/101‑1 (MQ=255)


                             gCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                         <  1:4328309/101‑1 (MQ=255)


                               cATGTCGCTCCCCCCGCGGGGGCGTGGATCGACAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  2:5263506/101‑1 (MQ=255)


                               cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  1:4075646/101‑1 (MQ=255)


                               cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       >  2:504336/1‑101 (MQ=255)


                               cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                       <  2:1954459/101‑1 (MQ=255)


                               cATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCAACTCAGCCCCGCCCTCTGACTAACCCACAGCCGCTACCCACGCGGGGGCGGggctagagg                                                                       >  2:3338665/1‑94 (MQ=18)


                                aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      >  2:5562051/1‑101 (MQ=255)


                                aTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                      >  2:13231/1‑101 (MQ=255)


                                 tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                     >  1:5009201/1‑101 (MQ=255)


                                 tGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                     <  1:549434/101‑1 (MQ=255)


                                   tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAActct                                                                   >  2:647751/1‑101 (MQ=255)


                                    cGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCNCCCCCCGCGGGGGCGTGGATCGAAACTCTa                                                                  >  1:228197/1‑101 (MQ=255)


                                        ccccccGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATggg                                                              >  1:2072902/1‑101 (MQ=255)


                                             cgcgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggct                                                         <  1:6969335/101‑1 (MQ=255)


                                              gcgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggcta                                                        <  2:3152619/101‑1 (MQ=255)


                                               cgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                       >  2:4301598/1‑101 (MQ=255)


                                               cgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                       >  2:3534325/1‑101 (MQ=255)


                                               cgGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctag                                                       >  1:6263405/1‑101 (MQ=255)


                                                gggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagg                                                      >  1:1511420/1‑101 (MQ=255)


                                                 ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggg                                                     <  2:6157412/101‑1 (MQ=255)


                                                 ggggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaagatcgga                                                     >  1:3424965/1‑93 (MQ=255)


                                                  gggCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctaggga                                                    <  1:5527550/101‑1 (MQ=255)


                                                     cGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggc                                                 >  2:5738849/1‑101 (MQ=255)


                                                       tGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                               <  2:4373698/101‑1 (MQ=255)


                                                       tGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATgggaggctagggaggcta                                               <  2:4248095/101‑1 (MQ=255)


                                                        ggATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                              >  2:4715260/1‑101 (MQ=255)


                                                        ggAACGAACC.CCGGCACCGCAGCCCCGCCCGCTGTCTCACCAACAGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTAc                                              <  2:6953457/101‑1 (MQ=38)


                                                          aTCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGc                                            >  1:1848330/1‑101 (MQ=255)


                                                           tCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                           >  1:1259587/1‑101 (MQ=255)


                                                           tCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCa                                           >  1:3572205/1‑101 (MQ=255)


                                                            cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                                          <  1:3664832/101‑1 (MQ=255)


                                                            cGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAc                                          >  1:6021263/1‑101 (MQ=255)


                                                             gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                                         >  1:946481/1‑101 (MQ=255)


                                                             gAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCAcc                                         <  1:5562341/101‑1 (MQ=255)


                                                              aaaC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCt                                        >  2:29573/1‑101 (MQ=255)


                                                                    cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                                   <  2:6710397/101‑1 (MQ=255)


                                                                    cGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCaaa                                   <  1:4937451/101‑1 (MQ=255)


                                                                      gCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAg                                 >  1:1672027/1‑101 (MQ=255)


                                                                       cACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGAGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGt                                <  1:458928/101‑1 (MQ=255)


                                                                            cAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTc                           <  1:4362473/101‑1 (MQ=255)


                                                                             aGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcc                          <  1:4477371/101‑1 (MQ=255)


                                                                               ccccG.CCGGTGGCTCCCCCACCGGCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc                       <  1:7082655/101‑1 (MQ=25)


                                                                                cccgcccgCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTccccc                       <  2:4129216/101‑1 (MQ=255)


                                                                                 ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc                      <  1:2737629/101‑1 (MQ=255)


                                                                                 ccgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATCGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTcccccc                      <  2:3442972/101‑1 (MQ=255)


                                                                                  cgcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcg                     <  2:251743/101‑1 (MQ=255)


                                                                                   gcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCcgc                    >  2:5327960/1‑101 (MQ=255)


                                                                                   gcccgcTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCGACGCACCTTCAAAAGTCGCTCCCCCcgc                    >  1:6179776/1‑101 (MQ=255)


                                                                                        cTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCggggg               <  2:3632893/101‑1 (MQ=255)


                                                                                         tctctcCCCAACAGTCGCTCCCCCCGNNNNNGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc              <  1:3768468/99‑1 (MQ=255)


                                                                                         tGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGc              >  2:6662915/1‑101 (MQ=255)


                                                                                             tcACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTgg          <  1:1089437/101‑1 (MQ=255)


                                                                                              cACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGATAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGa         >  2:3082521/1‑101 (MQ=255)


                                                                                               aCCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGAt        <  1:4233731/101‑1 (MQ=255)


                                                                                                 cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCCCCTTCAAAAGTCGCTCCCCCCCCGGGGTCgcgtctcg      >  2:6862321/1‑94 (MQ=37)


                                                                                                 cAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCg      >  1:2355049/1‑101 (MQ=255)


                                                                                                  aaCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGa     >  2:2833162/1‑101 (MQ=255)


                                                                                                    cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa   >  1:5849390/1‑101 (MQ=255)


TCCAGCGTCACATGCAGCGGCGTCGCATCGCCATGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.CCGGCACCTCAGCCCCGCCCGCTGTCTCACCAACAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCTATGGGAGGCTAGGGAGGCTACGCACCTTCAAAAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC  >  NC_014034/1397802‑1398002

Base quality scores:


ATCG

< 3 ≤


ATCG

< 23 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG