Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,412,228 C→G 7.5% C534S (TGT→TCT)  RCAP_rcc01318 ← outer membrane autotransporter barrel domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,412,2280C→G 7.5% 65.0 ‑0.8 ‑0.4412C534S (TGT→TCT) RCAP_rcc01318outer membrane autotransporter barrel domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (13/18):  ref base (192/189):  total (205/207)
Fisher's exact test strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.13e-01

Only 100 of 462 total aligned reads displayed.

GCCCGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCGT  >  NC_014034/1412128‑1412328
                                                                                                    |                                                                                                    
gCCCGCGATCGGCGCGGAGAAGGTCTTTCCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGac                                                                                                      <  1:6510613/101‑1 (MQ=255)
 cccGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGaca                                                                                                     >  2:864660/1‑101 (MQ=255)
 cccGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGaca                                                                                                     <  2:6111871/101‑1 (MQ=255)
 cccGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGaca                                                                                                     <  2:271163/101‑1 (MQ=255)
  ccGCGATCGGCGCGGAGAAGGTCTTTCCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGacac                                                                                                    <  1:2254599/101‑1 (MQ=255)
  ccGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGacac                                                                                                    <  1:6604753/101‑1 (MQ=255)
   cgcgATCGGCGGGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGAcaca                                                                                                   <  1:2132441/101‑1 (MQ=255)
   cgcgATCGGCGCGGAGAAGGTCTTTCCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGAcaca                                                                                                   <  2:4746611/101‑1 (MQ=255)
    gcgATGGGCGGGGAAAAGGTCTTTACCCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAg                                                                                                  <  1:4424328/101‑1 (MQ=18)
     cgctgggCGCGGAGACGGTCTTGACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAgg                                                                                                 <  2:4167682/96‑1 (MQ=255)
      ggtgggCGGGGAAAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGc                                                                                                <  2:6917596/97‑1 (MQ=255)
      gATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGc                                                                                                >  1:4907865/1‑101 (MQ=255)
       aTCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGcc                                                                                               >  1:6759780/1‑101 (MQ=255)
        tCGGCGGGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCg                                                                                              <  1:1304145/101‑1 (MQ=255)
         cGGCGGGGAGAAGGTTTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGt                                                                                             <  1:948952/101‑1 (MQ=255)
          ggCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCgac                                                                                            >  1:2302688/1‑99 (MQ=255)
           gcgcGGAGAAGTTCTTTACCCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGtct                                                                                           <  1:3164338/101‑1 (MQ=255)
              gggAGAGGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTccc                                                                                        <  2:656513/100‑1 (MQ=255)
              cgGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTccc                                                                                        >  2:1478529/1‑101 (MQ=255)
              cgGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTccc                                                                                        >  1:6450462/1‑101 (MQ=255)
               ggAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCcg                                                                                       >  1:2583567/1‑101 (MQ=255)
               ggAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCcg                                                                                       >  1:4046475/1‑101 (MQ=255)
                  gaAGGTCTTTCCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCgg                                                                                    <  1:1548358/101‑1 (MQ=255)
                    acgTCTTTCCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCa                                                                                  <  2:2170672/99‑1 (MQ=255)
                     ggTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCAt                                                                                 >  2:5510359/1‑101 (MQ=255)
                       tCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGAGACAGGCCGTCTCCcgcgcggtag                                                                               >  2:444609/1‑95 (MQ=255)
                       tCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAg                                                                               >  2:2950307/1‑101 (MQ=255)
                       tCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAg                                                                               >  2:1012345/1‑101 (MQ=255)
                       tCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAg                                                                               >  1:1604326/1‑101 (MQ=255)
                       tCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGCCATac                                                                               >  1:3333184/1‑100 (MQ=255)
                        tttaccccccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAgg                                                                              <  2:4082019/94‑1 (MQ=255)
                        cTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAgg                                                                              >  1:4312427/1‑101 (MQ=255)
                         tttccccccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAggt                                                                             <  1:4737447/95‑1 (MQ=255)
                          ttccgcccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAggtg                                                                            <  2:3715557/98‑1 (MQ=255)
                            aCGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAggtggt                                                                          >  2:4912942/1‑101 (MQ=255)
                              gcccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGc                                                                        >  1:3813266/1‑101 (MQ=255)
                               cccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGcc                                                                       <  2:2735717/101‑1 (MQ=255)
                                ccgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGccc                                                                      >  2:5472289/1‑101 (MQ=255)
                                 cgccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCa                                                                     >  1:3244313/1‑101 (MQ=255)
                                   ccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCAcc                                                                   <  1:7104719/101‑1 (MQ=255)
                                   ccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCAcc                                                                   <  1:5699591/101‑1 (MQ=255)
                                   ccAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCAcc                                                                   <  2:5274612/101‑1 (MQ=255)
                                    cAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcg                                                                  <  2:5193068/101‑1 (MQ=255)
                                     aGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgc                                                                 >  1:629934/1‑101 (MQ=255)
                                     aGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgc                                                                 <  2:6759426/101‑1 (MQ=255)
                                      gtgtCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACcgcg                                                                >  2:1058832/1‑101 (MQ=255)
                                       tgtCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCgcgc                                                               >  1:4788515/1‑101 (MQ=255)
                                         tCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGccc                                                             <  2:2798390/101‑1 (MQ=255)
                                         gcTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGccc                                                             <  1:5742828/100‑1 (MQ=255)
                                          cTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcg                                                            >  2:3942176/1‑101 (MQ=255)
                                            gATCGCCCGAGGTGCGCACCACGGTGCCGTCATCTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcgcg                                                          <  1:3635635/101‑1 (MQ=255)
                                            gATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCcgcg                                                          <  1:3414684/101‑1 (MQ=255)
                                               cGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACt                                                       >  1:7032088/1‑101 (MQ=255)
                                               cGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACt                                                       >  2:1393205/1‑101 (MQ=255)
                                                gCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACtt                                                      >  1:1455870/1‑101 (MQ=255)
                                                 cccGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTg                                                     <  2:353593/101‑1 (MQ=255)
                                                     aGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCg                                                 >  2:1850717/1‑101 (MQ=255)
                                                       gTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTc                                               <  2:32268/101‑1 (MQ=255)
                                                        tGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCa                                              <  2:3083992/101‑1 (MQ=255)
                                                        cgcgcACCCCCGTCCCGTCCCCTGCCAAATTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCa                                              <  2:2446333/100‑1 (MQ=11)
                                                          cgcACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAAc                                            <  2:6450132/101‑1 (MQ=255)
                                                           gcACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACa                                           <  1:10106/101‑1 (MQ=255)
                                                             accacGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATa                                         <  1:1415245/101‑1 (MQ=255)
                                                                 cGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGcc                                     >  1:4614113/1‑101 (MQ=255)
                                                                  ggTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCt                                    <  2:5823720/101‑1 (MQ=255)
                                                                   gTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCtt                                   <  1:6008037/101‑1 (MQ=255)
                                                                   gTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCtt                                   >  1:6109764/1‑101 (MQ=255)
                                                                   gTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCtt                                   <  2:1566537/101‑1 (MQ=255)
                                                                    tGCCGTCAACGGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTc                                  <  2:4346280/101‑1 (MQ=255)
                                                                     gCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCg                                 >  2:1950797/1‑101 (MQ=255)
                                                                      ccGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGa                                <  2:6268268/101‑1 (MQ=255)
                                                                      ccGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGa                                >  2:5998925/1‑101 (MQ=255)
                                                                       cGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAg                               >  1:868182/1‑101 (MQ=255)
                                                                          cAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGcc                            <  2:5142621/101‑1 (MQ=255)
                                                                           aaCTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCa                           >  2:7109560/1‑101 (MQ=255)
                                                                             cTGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCAcc                         <  2:1655094/101‑1 (MQ=9)
                                                                              tGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCt                        <  2:2600214/101‑1 (MQ=255)
                                                                              tGCGAAAGTGGTGCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGGCTTGGGCGTCAACATAGGCCTTCGAGGCCACCt                        <  1:3469923/101‑1 (MQ=9)
                                                                               gCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTg                       <  2:5900655/101‑1 (MQ=255)
                                                                                  aaGGTTGTTCCCGCAAGACCCAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGcc                    <  2:5694866/101‑1 (MQ=255)
                                                                                   aGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGccc                   <  1:5933551/101‑1 (MQ=255)
                                                                                    agtggtgCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCg                  >  1:1737517/2‑101 (MQ=14)
                                                                                      tggtgCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGca                >  1:5346198/1‑100 (MQ=11)
                                                                                      gggtgCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGcc                <  2:4681124/100‑1 (MQ=255)
                                                                                       ggtgCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCa               <  1:6226728/101‑1 (MQ=40)
                                                                                        gtgCCCGCAAGAGACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGcaag              <  1:5880760/101‑4 (MQ=2)
                                                                                        gtgCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAc              >  1:2978781/1‑101 (MQ=255)
                                                                                        gtgCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAc              >  1:2013447/1‑101 (MQ=255)
                                                                                        gtccccGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAc              <  1:1851327/98‑1 (MQ=255)
                                                                                            ccGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtc          <  2:202386/101‑1 (MQ=255)
                                                                                             cGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtcg         >  2:4784787/1‑101 (MQ=255)
                                                                                              gCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtcgg        >  1:5156574/1‑101 (MQ=255)
                                                                                              gCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtcgg        >  1:1089709/1‑101 (MQ=255)
                                                                                               cAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCAcggtcggt       >  1:2754496/1‑101 (MQ=255)
                                                                                               cAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCAAGGCCACATAGCCCGCCAcggtctgt       >  2:7085633/1‑101 (MQ=17)
                                                                                                aaGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTg      >  2:3379349/1‑101 (MQ=255)
                                                                                                 aGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGc     >  2:68203/1‑101 (MQ=255)
                                                                                                  gACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGcc    <  1:5250417/101‑1 (MQ=255)
                                                                                                  gACACAGGCCGTCCCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGcc    <  2:2583471/101‑1 (MQ=255)
                                                                                                   acacAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCg   >  1:5561395/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCCCGCGATCGGCGCGGAGAAGGTCTTTACGCCCGCCAGTGTCTGATCGCCCGAGGTGCGCACCACGGTGCCGTCAACTGCGAAGGTGGTGCCCGCAAGACACAGGCCGTCTCCCGCGGCATAGGTGGTGCCCACCGCGCCCGCGACTTGGGCGTCAACATAGGCCTTCGAGGCCACCTGGCCCGCCACGGTCGGTGCCGT  >  NC_014034/1412128‑1412328

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG