Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,434,412 C→G 17.3% intergenic (‑191/‑767) RCAP_rcc01334 ← / → RCAP_rcc01335 hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,434,4120C→G17.3% 177.3 0.0 ‑0.0410intergenic (‑191/‑767)RCAP_rcc01334/RCAP_rcc01335hypothetical protein/Fis family GAF modulated sigma54 specific transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (31/40):  ref base (146/190):  total (178/232)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 417 total aligned reads displayed.

AAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434312‑1434510
                                                                                                       |                                                                                                  
aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                                    <  1:6526121/101‑1 (MQ=255)
aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                                    <  2:7108495/101‑1 (MQ=255)
aaaCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAAcc                                                                                                    <  1:5574662/101‑1 (MQ=255)
  aCAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCtt                                                                                                  <  2:5637135/101‑1 (MQ=255)
   cAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCAGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTg                                                                                                 >  2:2916398/1‑101 (MQ=255)
    aGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGt                                                                                                <  2:6482037/101‑1 (MQ=255)
     ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTc                                                                                               >  2:6924355/1‑101 (MQ=255)
     ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTc                                                                                               <  1:2700473/101‑1 (MQ=255)
     ggCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTc                                                                                               <  1:3475929/101‑1 (MQ=255)
       ctgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGc                                                                                             <  1:1926842/101‑1 (MQ=255)
        tgctTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCt                                                                                            >  1:5201177/1‑101 (MQ=255)
             tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                                       >  2:3899198/1‑101 (MQ=255)
             tGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTccccc                                                                                       <  2:5352358/101‑1 (MQ=255)
               ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcg                                                                                     <  2:1353455/101‑1 (MQ=255)
               ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcg                                                                                     <  2:5111673/101‑1 (MQ=255)
               ggCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCcg                                                                                     <  1:705157/101‑1 (MQ=255)
                    aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgggg                                                                                <  2:4098128/101‑1 (MQ=255)
                    aaCACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCgggg                                                                                <  2:934304/101‑1 (MQ=255)
                      cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                                              <  1:6242090/101‑1 (MQ=255)
                      cacCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGc                                                                              <  1:4590886/101‑1 (MQ=255)
                         cTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTg                                                                           <  1:673571/101‑1 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:6803676/101‑1 (MQ=255)
                             tCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                       <  1:4230136/101‑1 (MQ=255)
                              gggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGa                                                                         <  1:5417769/93‑1 (MQ=2)
                              ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  1:116341/101‑1 (MQ=255)
                              ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:3700412/101‑1 (MQ=255)
                              ccTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      <  2:6358013/101‑1 (MQ=255)
                               ggctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGag                                                                        >  1:315830/8‑100 (MQ=2)
                                tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGGTCGaa                                                                    >  1:2172507/1‑101 (MQ=255)
                                tgctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    <  2:152613/101‑1 (MQ=255)
                                 gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  2:4193415/1‑101 (MQ=255)
                                 gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   >  1:6948446/1‑101 (MQ=255)
                                 gctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:898962/101‑1 (MQ=255)
                                 ctatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                      >  1:719225/6‑101 (MQ=2)
                                  tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     <  1:6789447/97‑1 (MQ=2)
                                  tatcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                     >  1:5803523/5‑101 (MQ=2)
                                  ctgGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:1894252/101‑1 (MQ=255)
                                   atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  2:4521149/4‑101 (MQ=2)
                                   atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                    >  2:5269804/4‑101 (MQ=2)
                                   atcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                                                    >  1:3867631/4‑101 (MQ=2)
                                    tcgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                   <  1:6424360/99‑1 (MQ=2)
                                    gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacac                                                                >  1:1682638/1‑101 (MQ=255)
                                    gggTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGCGGATCGAAacac                                                                >  1:4492464/1‑101 (MQ=255)
                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  >  1:2534251/2‑101 (MQ=2)
                                     cgTCGCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  2:5966639/100‑1 (MQ=2)
                                     cgTCGCCCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAac                                                                  <  1:3025360/100‑1 (MQ=2)
                                        cGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGAt                                                            >  1:99368/1‑101 (MQ=255)
                                         gCTCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctg                                                              >  1:203578/1‑97 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgct                                                            <  2:5783081/101‑7 (MQ=2)
                                           tCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGc                                                         <  1:862636/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGTGTGGATCGAAacgctgctg                                                           <  2:3255055/101‑8 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctg                                                           >  2:2428367/1‑94 (MQ=2)
                                            ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                        >  2:6570723/1‑101 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                        <  2:1622147/101‑1 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCa                                                        >  1:3742732/1‑101 (MQ=255)
                                            ccccccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACACGGATTGCa                                                        >  2:494319/1‑101 (MQ=255)
                                             cccccGCGGGGGCGTGGATCGAAACGGGTCGAAGACCTGCTTGACGCGCTGGATCTGCGGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAacgctgctga                                                          <  1:2841235/101‑9 (MQ=2)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    <  2:744805/101‑1 (MQ=255)
                                                ccGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgc                                                    <  1:3629205/101‑1 (MQ=255)
                                                 cgcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcg                                                   <  1:5032315/101‑1 (MQ=255)
                                                  gcgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTCCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGgcgc                                                  >  2:3455053/1‑101 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  2:384800/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  1:5301374/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  2:6130679/101‑1 (MQ=255)
                                                   cgGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGcc                                                 <  2:6744880/101‑1 (MQ=255)
                                                    gggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCg                                                >  1:4884295/1‑101 (MQ=255)
                                                     ggggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGa                                               >  2:2028751/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  2:1758530/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              >  2:4392697/1‑101 (MQ=255)
                                                      gggCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAg                                              <  1:4331399/101‑1 (MQ=255)
                                                        gCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAggg                                            <  1:39290/101‑1 (MQ=255)
                                                          gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          >  1:1572355/1‑101 (MQ=255)
                                                          gTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGAc                                          <  1:1009773/101‑1 (MQ=255)
                                                            ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGTGCCGAGGGACAg                                        <  2:5200912/101‑1 (MQ=255)
                                                            ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        <  1:4793994/101‑1 (MQ=255)
                                                            ggATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAg                                        >  1:6103985/1‑101 (MQ=255)
                                                              aTCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGc                                      <  2:2555229/101‑1 (MQ=255)
                                                               tCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCt                                     >  2:6220441/1‑101 (MQ=255)
                                                                cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    >  2:3590540/1‑101 (MQ=255)
                                                                cGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCtt                                    >  2:2797578/1‑101 (MQ=255)
                                                                 gAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCttt                                   >  1:1369623/1‑101 (MQ=255)
                                                                  aaaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTg                                  <  2:5030258/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 <  1:6667307/101‑1 (MQ=255)
                                                                   aaC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGc                                 >  1:2337575/1‑101 (MQ=255)
                                                                     c..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAg                               <  2:6634028/101‑1 (MQ=255)
                                                                         tctcCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTc                             <  2:1682577/101‑1 (MQ=255)
                                                                            ccGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt                          <  1:3258457/101‑1 (MQ=255)
                                                                            ccGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCt                          <  1:2965033/101‑1 (MQ=255)
                                                                                tctGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                      <  2:3155022/101‑1 (MQ=255)
                                                                                tctGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccc                      <  1:6168929/101‑1 (MQ=255)
                                                                                 ctGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTccccc                     <  1:2603491/101‑1 (MQ=255)
                                                                                  tGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  2:2712561/101‑1 (MQ=255)
                                                                                  tGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTcccccc                    <  2:6550090/101‑1 (MQ=255)
                                                                                   gCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcg                   <  1:4414921/101‑1 (MQ=255)
                                                                                     ttCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCcccg                 >  2:7038215/1‑101 (MQ=255)
                                                                                          g.gCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGc            <  1:5512544/101‑1 (MQ=40)
                                                                                            gCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCg           <  2:2166853/101‑1 (MQ=40)
                                                                                             cTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCgg          >  1:4277237/1‑100 (MQ=39)
                                                                                               gCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTgg        <  1:5881537/101‑1 (MQ=37)
                                                                                                cGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGa       >  2:3369642/1‑101 (MQ=35)
                                                                                                       |                                                                                                  
AAACAGGCTGCTTTGGGCGGAACACCTGATCCTGCTGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GTCTCCGCTCTGCTTCACG.GCTGCGTCAACCTTGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACGGATTGCATGGCGCCGAGGGACAGGCTTTGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAA  >  NC_014034/1434312‑1434510

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG