Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,443,778 G→C 100% A33A (GCC→GCG RCAP_rcc01340 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,443,7780G→C100.0% 1109.5 297A33A (GCC→GCGRCAP_rcc01340LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (162/135):  ref base (0/0):  total (162/135)

Only 100 of 312 total aligned reads displayed.

GCCGCGCCCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGGTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCA  >  NC_014034/1443680‑1443875
                                                                                                  |                                                                                                 
gccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGc                                                                                                 <  2:6594329/101‑1 (MQ=255)
gccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGc                                                                                                 <  1:4664929/101‑1 (MQ=255)
 ccgcgccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGGGATCGTCGCa                                                                                                >  2:2117943/1‑101 (MQ=255)
     gccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGc                                                                                            >  1:3242094/1‑101 (MQ=255)
     gccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGc                                                                                            >  2:448872/1‑101 (MQ=255)
     gccCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGc                                                                                            >  1:1447162/1‑101 (MQ=255)
        cTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCac                                                                                         >  2:722324/1‑100 (MQ=255)
        cTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGGCGCAACGCCaa                                                                                         >  2:3935598/1‑101 (MQ=255)
         tGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAg                                                                                        >  1:4455973/1‑101 (MQ=255)
          ggtcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCAGCCGCTCGATGCGCCGGGAGACCGTCGCAACGCCAAgc                                                                                       >  1:6481906/1‑100 (MQ=255)
           gtcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACCCCAAGAc                                                                                      >  2:4598258/1‑101 (MQ=255)
            tcggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAcc                                                                                     >  2:5362834/1‑101 (MQ=255)
             cggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAccc                                                                                    <  2:3569125/101‑1 (MQ=255)
             cggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGAccc                                                                                    <  1:256506/101‑1 (MQ=255)
              ggtcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCa                                                                                   <  1:1492038/101‑1 (MQ=255)
                tcAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGt                                                                                 <  1:5752015/101‑1 (MQ=255)
                tcAGTTCGGGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGt                                                                                 <  1:4387824/101‑1 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                <  2:3692774/101‑1 (MQ=255)
                 cAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTc                                                                                <  2:1634001/101‑1 (MQ=255)
                  aGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcg                                                                               >  2:1317980/1‑101 (MQ=255)
                    ttCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCAGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgcc                                                                             <  1:1104673/101‑1 (MQ=255)
                       gTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgcg                                                                          >  1:6267884/1‑101 (MQ=255)
                       gTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTcgccgcg                                                                          <  2:2079848/101‑1 (MQ=255)
                          cccGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCCCAACGCCAAGACCCAGTcgccgcgccg                                                                       >  2:6358559/1‑101 (MQ=255)
                           ccGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCgccgcgccgc                                                                      >  2:1868527/1‑101 (MQ=255)
                           ccGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCgccgcgccgc                                                                      >  1:3106596/1‑101 (MQ=255)
                             ggTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCcgcgccgcgc                                                                    >  2:5160125/1‑101 (MQ=255)
                              gTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCgcgccgcgcc                                                                   >  2:6873097/1‑101 (MQ=255)
                              gTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCgcgccgcgcc                                                                   >  1:13084/1‑101 (MQ=255)
                                cTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCt                                                                 <  1:6117012/101‑1 (MQ=255)
                                cTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCt                                                                 >  2:4194977/1‑101 (MQ=255)
                                cTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCt                                                                 >  1:4247216/1‑101 (MQ=255)
                                cTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCt                                                                 >  1:3932259/1‑101 (MQ=255)
                                  ggcggcGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGGCGCAACGCCAAGGCCCAGGCGCCGCGCCGCGCCCTGc                                                               >  2:3830806/1‑101 (MQ=255)
                                     ggcgCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCCCTCGAATCTCCGCGAGATCGTCGGAACGCCAAGACCCAGTCGCCTCGCCGCGCCCTGCAGc                                                            >  2:6507109/1‑101 (MQ=255)
                                        gcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCgcg                                                         >  1:4522311/1‑99 (MQ=255)
                                        gcAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTg                                                         >  1:2121695/1‑101 (MQ=255)
                                         cAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGc                                                        <  1:2659410/101‑1 (MQ=255)
                                           ggAACGGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGccc                                                      >  2:4840110/1‑101 (MQ=255)
                                           ggAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGccc                                                      >  1:4128046/1‑101 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     >  2:4177703/1‑101 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     <  2:1365584/101‑1 (MQ=255)
                                            gAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCt                                                     >  1:3344649/1‑101 (MQ=255)
                                             aaCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTc                                                    >  2:6109605/1‑101 (MQ=255)
                                              aCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcg                                                   >  1:3635694/1‑101 (MQ=255)
                                              aCAGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcg                                                   >  1:1351832/1‑101 (MQ=255)
                                                aGCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcg                                                 >  2:1021076/1‑101 (MQ=255)
                                                 gCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcgc                                                >  2:5817683/1‑101 (MQ=255)
                                                 gCGGCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTcgcgc                                                >  2:2842857/1‑101 (MQ=255)
                                                    gCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcc                                             >  2:4086136/1‑101 (MQ=255)
                                                    gCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcc                                             <  2:3073870/101‑1 (MQ=255)
                                                    gCAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcc                                             <  2:3046203/101‑1 (MQ=255)
                                                     cAGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgcca                                            >  1:1909551/1‑101 (MQ=255)
                                                      aGGCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccag                                           >  1:2400739/1‑101 (MQ=255)
                                                       ggCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagc                                          <  1:2972243/101‑1 (MQ=255)
                                                       ggCCGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagc                                          >  1:3786383/1‑101 (MQ=255)
                                                        gCCGAGCGTCGCCTCCATCCGCTCGATGCCCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcg                                         <  2:2974249/101‑1 (MQ=255)
                                                          cGAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagcgcc                                       >  2:553701/1‑101 (MQ=255)
                                                           gAGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCgcgccagagcca                                      <  2:5971146/101‑1 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     >  1:1200702/1‑101 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     <  2:491023/101‑1 (MQ=255)
                                                            aGCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGcgccagcgccag                                     <  1:2117130/101‑1 (MQ=255)
                                                             gCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGa                                    >  2:7058647/1‑101 (MQ=255)
                                                             gCGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGa                                    >  1:878336/1‑101 (MQ=255)
                                                              cGTCGCCTCCATGCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCCGCGTGCCCTCGCGCACCAGCGCCAGaa                                   >  1:5167386/1‑101 (MQ=255)
                                                              cGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGaa                                   >  1:554566/1‑101 (MQ=255)
                                                              cGTCGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGaa                                   <  1:1693951/101‑1 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                >  1:1541036/1‑101 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                >  2:1122845/1‑101 (MQ=255)
                                                                 cGCCTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACa                                <  2:2137751/101‑1 (MQ=255)
                                                                    cTCCATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGcc                             >  2:1825801/1‑101 (MQ=255)
                                                                       cATCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGa                          <  2:6036096/101‑1 (MQ=255)
                                                                        atcccctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGCAACAGCCGGAt                         >  2:1144578/7‑101 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         <  1:4213527/101‑1 (MQ=255)
                                                                        aTCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAt                         >  1:5400011/1‑101 (MQ=255)
                                                                         tCCGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATg                        <  2:4977272/101‑1 (MQ=255)
                                                                          ccGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgt                       >  1:747702/1‑101 (MQ=255)
                                                                          ccGCTCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgt                       <  1:4913094/101‑1 (MQ=255)
                                                                            gctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCCGATgtcg                     >  2:4327920/3‑101 (MQ=255)
                                                                             ctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtcgt                    <  1:5920009/100‑1 (MQ=255)
                                                                             ctCGATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtcgt                    <  2:1158744/100‑1 (MQ=255)
                                                                             ctCGAGGCGCCGCGAGATCGTCGCAACGCCAAGCCCCCGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATgtcgt                    <  1:3071135/100‑1 (MQ=255)
                                                                                gATGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 >  2:3254330/1‑101 (MQ=255)
                                                                                gATGCGCCGCGAAATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCGCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTccc                 >  1:5096126/1‑101 (MQ=255)
                                                                                 aTGCGCCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCa                <  1:3461848/101‑1 (MQ=255)
                                                                                   gcgcCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGt              >  1:5318721/1‑101 (MQ=255)
                                                                                   gcgcCGCGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGt              <  2:2639169/101‑1 (MQ=255)
                                                                                    cgccgcGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGtt             <  1:1840188/101‑1 (MQ=255)
                                                                                      ccgcGAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCt           <  2:5649779/101‑1 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         >  2:6796898/1‑101 (MQ=255)
                                                                                        gcgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCtgt         <  1:7009592/101‑1 (MQ=255)
                                                                                         cgAGATCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCCCCAGAAACAGCCGGATGTCGTCCCAGTTCtgtg        <  1:5016861/101‑1 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       >  1:6690958/1‑101 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       <  2:3240054/101‑1 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       >  2:2530139/1‑101 (MQ=255)
                                                                                          gagaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTgg       <  1:6909401/101‑1 (MQ=255)
                                                                                           agaTCGTCGCAACGCCAAGACCCAGTCGCCGCGACGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGc      >  1:3712932/1‑101 (MQ=255)
                                                                                            gaTCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCt     <  1:227090/101‑1 (MQ=255)
                                                                                              tCGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTcc   <  2:5769182/101‑1 (MQ=255)
                                                                                               cGTCGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGAGGTCGCCCCAGTTCTTTGGCTccc  >  2:2317658/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
GCCGCGCCCTGGTCGGTCAGTTCGTGCCCGGTCTGGCGGCGCAGGAACAGCGGCAGGCCGAGCGTCGCCTCCATCCGGTCGATGCGCCGCGAGATCGTGGCAACGCCAAGACCCAGTCGCCGCGCCGCGCCCTGCAGCGTGCCCTCGCGCGCCAGCGCCAGAAACAGCCGGATGTCGTCCCAGTTCTGTGGCTCCA  >  NC_014034/1443680‑1443875

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG