Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,612,428 T→C 1.8% intergenic (+106/‑255) purN → / → rnd2 phosphoribosylglycinamide formyltransferase/ribonuclease D

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,612,4280T→C 1.8% 3.2 ‑2.8 ‑0.8281intergenic (+106/‑255)purN/rnd2phosphoribosylglycinamide formyltransferase/ribonuclease D
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (136/140):  total (141/140)
Fisher's exact test strand distribution p-value = 6.03e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.56e-01

Only 100 of 307 total aligned reads displayed.

ATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAGGCGA  >  NC_014034/1612328‑1612528
                                                                                                    |                                                                                                        
 tCCGCTGATCTGCCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATg                                                                                                         <  1:6310210/101‑1 (MQ=255)
 tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATg                                                                                                         <  2:82282/101‑1 (MQ=255)
 tCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATg                                                                                                         <  2:4598632/101‑1 (MQ=255)
  ccGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATgg                                                                                                        >  1:1954744/1‑101 (MQ=255)
   cGCTGATCTGCCCGAGGCCACCGCCGAGGTCCCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggc                                                                                                       <  1:6007483/101‑1 (MQ=255)
    gCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcg                                                                                                      >  2:5870447/1‑101 (MQ=255)
      tGATCTGACGGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcggg                                                                                                    <  2:5181151/101‑1 (MQ=255)
       gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggg                                                                                                   >  2:4145255/1‑100 (MQ=255)
       gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggc                                                                                                   >  2:14022/1‑101 (MQ=255)
       gATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggc                                                                                                   >  1:5318870/1‑101 (MQ=255)
        aTCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATggcgggcg                                                                                                  >  2:3422369/1‑101 (MQ=255)
           tGACCGAGGCCATCGCCGGGGTCACGCGACACATGGGCCCCGGGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAt                                                                                               <  1:1209334/101‑1 (MQ=17)
           tGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGgggccc                                                                                               >  2:6252365/1‑99 (MQ=255)
            gACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCact                                                                                              >  2:1591532/1‑99 (MQ=255)
            gACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCAtt                                                                                              <  2:6848139/101‑1 (MQ=255)
             ccggAGGCCATCGCGGAGGCCACGCGACACATTGGCCCCGGGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTc                                                                                             <  2:1973145/98‑1 (MQ=14)
             cccGGGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTc                                                                                             <  1:104128/100‑1 (MQ=255)
             cccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTc                                                                                             <  2:6130203/100‑1 (MQ=255)
             acgggggCCACCGCCGAGGCCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTc                                                                                             <  2:6977366/96‑1 (MQ=255)
             aCCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGGCTGCGCCCGCCCCGCCCATGGCGGgcgccatc                                                                                             >  1:3551666/1‑97 (MQ=255)
              ccGAGGCCATCGCCGAGGTCACGCGACCCATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCCTGGCGGGCGGATTcg                                                                                            <  1:6860564/101‑1 (MQ=255)
              ccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGGAGTcg                                                                                            >  1:246678/1‑101 (MQ=255)
              ccGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTcg                                                                                            >  1:4860788/1‑101 (MQ=255)
               cGAGGCCATCGCCGAGGTCAAGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTcgc                                                                                           <  1:5607549/101‑1 (MQ=255)
                   gCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCCCTCGcgccc                                                                                       >  1:6503130/1‑100 (MQ=255)
                    ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcgccgc                                                                                      <  1:6140533/101‑1 (MQ=255)
                    ccATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGcgcccc                                                                                      >  2:2978770/1‑99 (MQ=255)
                     cATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCACTCGCgccgcc                                                                                     >  1:562013/1‑101 (MQ=255)
                        cGCCGAGGTCACGCGACACATGGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCggg                                                                                  <  1:5255792/101‑1 (MQ=255)
                        cGCCGAGGCCCCGCGACCCATTGGCCCCGTGCCCGCCGGGGGCCCGGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCggg                                                                                  <  1:3422493/101‑1 (MQ=14)
                         gCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCCCCgcgg                                                                                 >  2:719879/1‑101 (MQ=255)
                          ccGGGGTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGc                                                                                <  1:730456/101‑1 (MQ=255)
                           cGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCcggggg                                                                               >  2:1879688/1‑99 (MQ=255)
                               gTCACGCGACACATTGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGAc                                                                           <  1:5471626/101‑1 (MQ=255)
                               gTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGAc                                                                           <  2:6401341/101‑1 (MQ=255)
                                tCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTAGCGGCGCCGCGGCGGGGCg                                                                          >  2:1466490/1‑101 (MQ=17)
                                 cACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCGCCCCCCGGGCggggggc                                                                         >  1:190889/1‑97 (MQ=17)
                                  ccgcgGCACATTGGCCCGGTGCCCGCCGGGGGCCGAGGGGCCCTGGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCa                                                                        <  1:2925108/100‑1 (MQ=17)
                                  ccgcgACCCATGGGCCCCGTGCCCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCa                                                                        <  1:3736460/100‑1 (MQ=255)
                                  aCGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCCCCGGGGCGCGGCGcc                                                                        >  2:6307702/1‑100 (MQ=255)
                                     cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCGCCGCCCGGGCggggggccggc                                                                     >  2:4085332/1‑93 (MQ=17)
                                     cgACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGc                                                                    >  2:4273665/1‑101 (MQ=255)
                                      gACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGGGCA.GGcc                                                                   >  1:3941304/1‑101 (MQ=255)
                                       acacATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCGCCGCCCGGGGGGGGGGCC.GGccc                                                                  >  1:4141499/1‑100 (MQ=14)
                                        cccaTTGGCCCCGTGCCCGCCGAGGGCCCAGAGCCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTc                                                                 <  1:2638810/99‑1 (MQ=255)
                                        cacaTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCGCCGCCCGGGGGGGGCGCA.CGCCTc                                                                 >  1:4655825/1‑101 (MQ=14)
                                         ccatggcccccGTGCCCGCCGGGGCCCCAGAGGCCCTCGCCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTcc                                                                <  2:2127091/94‑1 (MQ=14)
                                          caTTGGCCCCGTGCCCGCCGAGGCCCCAGGGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTccc                                                               <  2:3196730/101‑1 (MQ=255)
                                          caTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCCCAATCGCGCCGCCCGGGCGGGGCGCaaggccccc                                                                >  2:1156026/1‑93 (MQ=14)
                                           aTTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTTGCGCCGCCCGGGGGGGGCGCA.GGCCTCCcg                                                              >  1:4552278/1‑101 (MQ=14)
                                            ggctgaatgTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCGCCCCCGGGGCGGGACGCACGGCCCcccc                                                              >  2:1246596/9‑100 (MQ=14)
                                              ggCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCGCCGCCCGGGGGGGACGCA.GGGCTACcgcgc                                                           >  1:5840996/1‑101 (MQ=17)
                                              ggCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCcgcgc                                                           >  1:2567067/1‑101 (MQ=255)
                                              ggCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCCGGGGGGGGGGCA.GGCCCCCcccgc                                                           >  1:5022420/1‑101 (MQ=14)
                                               gtcctgTGACCGCCGAGGGCCCAAAGGCACTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGGAGCCGCGCCGCCCGGGGGGGGGGCG.GGGCCCCcgcttg                                                          >  2:5411837/6‑98 (MQ=14)
                                               gCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCCCCGGGGCGGGGCGCA.GGCCTCCcccgcg                                                          >  1:2051506/1‑101 (MQ=255)
                                                    gTGCCCGCCGAGGGCCCAGAGCCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTgg                                                    <  1:4784257/101‑1 (MQ=255)
                                                      gACCGCCGGGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGTGGGCTTGCCCCGCCGGGGCTGGGCGCG.CGGCTCCCGCGGG.CTTgtgg                                                  >  2:3116159/1‑101 (MQ=9)
                                                       cccgccgAGGGCCCAGAGCCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGa                                                 <  2:3059924/100‑1 (MQ=255)
                                                          gccgGGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGCGTTCGCGCCCCCCGGGCTGGGGGCG.GGGCCCCCTCGCGCCTTgggggaa                                               >  2:5240380/1‑98 (MQ=2)
                                                          gccgAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTTGCGCCGCCGGGGCGGGGCGCA.GGCCCCCCGCGCG.CCTGGGGGggg                                              >  2:4432461/1‑100 (MQ=14)
                                                           ccgAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCCGGGCGGGGCGCA.GGGCTCCCGCGCC.CCTGGGGGGGCg                                             >  2:675437/1‑101 (MQ=14)
                                                             gAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCCTTCGCGCCGCCGGGGCGGGGCGCA.GGCCCCCCCCGCGCCTTGGGGGggggt                                            >  1:1010485/1‑98 (MQ=14)
                                                             gAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGGGGGGCGCA.GGCCTCCCGCGCG.CCTGGGGGGGCggt                                           >  1:5774545/1‑99 (MQ=17)
                                                             gAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTACCGCCGCCCGGGCGGGGCGCA.CGCCCCCCGCCGG.CCTCGGGAGGCgcc                                           >  1:2080550/1‑99 (MQ=14)
                                                              aGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCCTTCCCGCCCCCGGGGCGGGGCGCA.CGCCCCCCCGGCC.GTTGGGGGggggcgc                                          >  1:1706896/1‑96 (MQ=2)
                                                              aGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCA.GGCCTCCCCCGCC.CCTGGGGGgggggtc                                          >  1:3130681/1‑96 (MQ=14)
                                                                ggCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGGCTCCCCCGCG.CCTCGGGGGGCGGTcct                                        >  2:3543191/1‑99 (MQ=17)
                                                                 gCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTCGCGCCGCCGGGGGGGGGCGCA.GGGCTCCCCCGCG.CGTGGGGGggggggtttg                                       >  1:6064187/1‑93 (MQ=14)
                                                                   ccgggggCCCGCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTc                                     <  1:6965768/96‑1 (MQ=255)
                                                                   ccggggccccTGGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTc                                     <  2:1040622/94‑1 (MQ=255)
                                                                   ccggagGCCCCCGGCCTGCCCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTc                                     <  1:2156518/98‑1 (MQ=255)
                                                                         gCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCC.GGCCTCCCCCGCG.CTTGGGGGGGCGATCTTGTCACCGGc                               >  1:6628916/1‑101 (MQ=255)
                                                                           ccTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCA.GGGCTCCCGCGCG.CTTGGGGGGGCGGTCTTGTCACCGGcgt                             >  2:3355743/1‑99 (MQ=17)
                                                                            cgcGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTg                            <  2:3941129/99‑1 (MQ=255)
                                                                            cTCGGCCTGCGCCCGCCCCGCCCATGGCGGGGGCATTTGCGCCGCCGGGGGGGGGCGCA.GGGCGCCCCCGCG.CGTGGGGGGGGGATCTTGTGCCCGGCGTg                            >  2:2262649/1‑101 (MQ=9)
                                                                             tCGGCGTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTgg                           <  1:2312348/101‑1 (MQ=255)
                                                                             gcGGCTTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTgg                           <  2:653752/100‑1 (MQ=255)
                                                                              cGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGt                          <  1:6402120/101‑1 (MQ=255)
                                                                               ggCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCA.GGCCTCCCGCGCG.CTTGGGGGGGCGATCTTGTCACCGGCtttggt                         >  1:4912489/1‑97 (MQ=255)
                                                                                gCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCC.GGCCTCCCCCGCG.CTTGGGGGGGCGATCTTGTCACCGGCTTTGTcg                        >  1:1576266/1‑101 (MQ=21)
                                                                                  cTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTcgc.g                     <  2:5329259/101‑1 (MQ=255)
                                                                                    gcgcCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAc                   <  2:4367814/101‑1 (MQ=255)
                                                                                    gcgcCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAc                   <  2:2566973/101‑1 (MQ=255)
                                                                                    gcccccgccccgcCCATGGCCGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAc                   <  1:4379773/98‑1 (MQ=255)
                                                                                         cgccccgcccATGGCGGGGGCCTTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CCTGGGGGGGCGGTCTTGTCCTCGGCTTGGTCCC.GGcggggg              >  2:2826321/1‑99 (MQ=18)
                                                                                          gccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAcggg.cgg            <  2:5814013/101‑1 (MQ=255)
                                                                                          gccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GAcggg.cgg            <  2:3588004/101‑1 (MQ=255)
                                                                                          gccccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGAAGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTTGTcgc.ggccggggg             >  2:2629930/1‑93 (MQ=25)
                                                                                            cccgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGGCGC.GGCGGG.Cgggg          >  2:4701896/1‑101 (MQ=255)
                                                                                              cgcccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGaa        <  1:7079703/101‑1 (MQ=255)
                                                                                                cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAg      <  2:565172/101‑1 (MQ=255)
                                                                                                cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAg      <  1:2424409/101‑1 (MQ=255)
                                                                                                cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAg      <  2:5513489/101‑1 (MQ=255)
                                                                                                cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAg      <  1:4474936/101‑1 (MQ=255)
                                                                                                cccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAg      <  1:802021/101‑1 (MQ=255)
                                                                                                 ccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTTGTCGC.GACCGG.CGGGGGAAgg     >  2:6459410/1‑101 (MQ=255)
                                                                                                 ccATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTTGGCGCGGCCGGG.GGGGGAAAg      >  2:3491919/1‑101 (MQ=25)
                                                                                                   aTGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAGGCg   <  2:5022167/101‑1 (MQ=255)
                                                                                                    tGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGG.GACGGGCCGGGGAAAgggg   >  2:6712196/1‑99 (MQ=255)
                                                                                                    |                                                                                                        
ATCCGCTGATCTGACCGAGGCCATCGCCGAGGTCACGCGACACATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCA.GGCCTCCCGCGCG.CTTGGGGAGGCGATCTTGTCACCGGCTTGGTCGC.GACGGG.CGGGGAAAGGCGA  >  NC_014034/1612328‑1612528

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG