Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,614,010 T→A 1.4% intergenic (+173/+54) rnd2 → / ← uvrD ribonuclease D/DNA helicase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,614,0100T→A 1.4% 3.7 ‑2.1 ‑1.4290intergenic (+173/+54)rnd2/uvrDribonuclease D/DNA helicase II
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (140/146):  total (140/150)
Fisher's exact test strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.55e-02

Only 100 of 308 total aligned reads displayed.

CCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGG  >  NC_014034/1613910‑1614110
                                                                                                      |                                                                                                     
ccTCGCCCGGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGt                                                                                                       <  2:1635296/101‑1 (MQ=255)
  tCGGCCGCCGCCCGCCCCGCCATCGGCGGGCGCATGCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTg                                                                                                     <  1:176264/101‑1 (MQ=255)
  gggcccggcgcCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTg                                                                                                     <  1:2519150/94‑1 (MQ=255)
   cGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCGTTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGc                                                                                                    <  1:3541058/101‑1 (MQ=255)
      ccTGCGCCCGCCCCGCCATCGGCGGGGGCCTTCGCG.CCCCCCGGGCGGGGCGCAGGGCCTTGTTGCGCTTTGGGGAACGGGCTTT.GCGCGGCGGTTGCggg                                                                                                 >  2:3887111/1‑99 (MQ=9)
       cTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATg                                                                                               <  2:1834593/101‑1 (MQ=255)
       cTGCCCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATg                                                                                               <  2:1406364/101‑1 (MQ=255)
         gggccggcccCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcg                                                                                             <  2:3093359/99‑1 (MQ=255)
         gcgcCCGCCCCGCCATCGGCGGGGGCATTTGCGCCCCCCGGGGGGGGGGGAGGGCCTTTTTTCGGCTTGGGG.AACGGGCTTG.CCGCGGCGGTTGCggggcg                                                                                              >  1:281016/1‑96 (MQ=12)
          gccccgccccgcCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATgcgg                                                                                            <  2:2184014/99‑1 (MQ=255)
               gccccgccATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGcgggcggg                                                                                       <  1:1814862/101‑1 (MQ=255)
                ccccgccATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCgggg                                                                                      <  1:4461360/101‑1 (MQ=255)
                ccccgccATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCgggg                                                                                      <  1:261571/101‑1 (MQ=255)
                ccccgccATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCgggg                                                                                      <  1:2362232/101‑1 (MQ=255)
                  ccgccATCGGCGGGCGCAGTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGaa                                                                                    <  2:2701234/101‑1 (MQ=255)
                   cgccATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGCCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGaaa                                                                                   <  1:5018192/101‑1 (MQ=255)
                    gccATCGGCGGGCGCATTCGCG.CCGCCGTGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAg                                                                                  <  1:4976017/101‑1 (MQ=255)
                     cccatggcgggcgCATTCGCG.CCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCCCCGGCTTTGGTG.GGGCCGGCggggggag                                                                                  >  2:4378672/6‑97 (MQ=18)
                      cATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGc                                                                                <  1:4890762/101‑1 (MQ=255)
                       aTCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCCGGCGGGGAAAGGCa                                                                               <  1:5571220/101‑1 (MQ=255)
                         cggcggGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGt                                                                             <  2:5750713/101‑1 (MQ=255)
                          ggcgggcgCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTg                                                                            <  1:809685/101‑1 (MQ=255)
                            cgggcgCAGTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGcc                                                                          <  2:3578674/101‑1 (MQ=255)
                             gggcgCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGccc                                                                         <  1:3343687/101‑1 (MQ=255)
                             gggcgCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGccc                                                                         >  2:769824/1‑101 (MQ=255)
                                cgcATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTc                                                                      >  1:344861/1‑101 (MQ=255)
                                 gcATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTcc                                                                     <  2:825627/101‑1 (MQ=255)
                                    ttCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTTCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGa                                                                  <  2:6194904/101‑1 (MQ=255)
                                     cgcgggcggccgGGGCGTCAGGAAGGCCTCTGTCGTGCTTGGGGAAACGGTCTTG.GCACAGGTGATGCGCCAGCGGGCGGGGAAAGGCAGTGCCTTTcccg                                                                   <  2:890976/93‑3 (MQ=25)
                                      cgcg.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATc                                                                <  1:4618934/101‑1 (MQ=255)
                                      cgcg.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATc                                                                >  2:5607202/1‑101 (MQ=255)
                                       gcg.cCGCCGGGGCGGGACGCAGGTCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATcc                                                               <  1:3156513/101‑1 (MQ=255)
                                       gcg.cCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATcc                                                               <  1:4351039/101‑1 (MQ=255)
                                         g.ccgccGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                                                             >  1:2026040/1‑101 (MQ=255)
                                         g.ccgccGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                                                             >  2:4969857/1‑101 (MQ=255)
                                             gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                          <  1:867787/101‑1 (MQ=255)
                                             gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                          >  1:5934710/1‑101 (MQ=255)
                                             gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                          <  1:2697120/101‑1 (MQ=255)
                                               cgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGaa                                                        >  2:1173655/1‑101 (MQ=255)
                                                ggggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAc                                                       >  1:6146028/1‑101 (MQ=255)
                                                 gggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                                                      <  2:3709033/101‑1 (MQ=255)
                                                  ggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCg                                                     <  2:3791433/101‑1 (MQ=255)
                                                    cGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                   <  1:1380394/101‑1 (MQ=255)
                                                    cGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                   <  2:2635670/101‑1 (MQ=255)
                                                      ggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc                                                 >  1:1075198/1‑101 (MQ=255)
                                                      ggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc                                                 >  2:2527650/1‑101 (MQ=255)
                                                       gACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCt                                                >  1:7016638/1‑101 (MQ=255)
                                                        aCGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGTCCGAACCGGGGCTc                                               >  1:54592/1‑101 (MQ=255)
                                                        aCGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTc                                               >  1:1664569/1‑101 (MQ=255)
                                                        aCGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTc                                               <  1:5762717/101‑1 (MQ=255)
                                                          gCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg                                             <  2:2259915/101‑1 (MQ=255)
                                                           cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                            >  2:6285186/1‑101 (MQ=255)
                                                           cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                            >  2:5836551/1‑101 (MQ=255)
                                                            aGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa                                           >  1:589228/1‑101 (MQ=255)
                                                             ggCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc                                          <  2:5356654/101‑1 (MQ=255)
                                                             ggCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc                                          >  2:4565582/1‑101 (MQ=255)
                                                             ggCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc                                          <  1:309929/101‑1 (MQ=255)
                                                               ccTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACgg                                        >  2:2971770/1‑101 (MQ=255)
                                                                cTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc                                       >  1:5189375/1‑101 (MQ=255)
                                                                cTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc                                       <  1:152215/101‑1 (MQ=255)
                                                                cTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc                                       >  2:6606395/1‑101 (MQ=255)
                                                                 tttGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCa                                      >  1:738981/1‑101 (MQ=255)
                                                                    gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                   >  2:4843928/1‑101 (MQ=255)
                                                                    gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                   >  2:3200918/1‑101 (MQ=255)
                                                                    gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgc                                   >  2:6393736/1‑100 (MQ=255)
                                                                    gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAAGGCAcgt                                   >  2:5112741/1‑101 (MQ=255)
                                                                    gttgCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTAATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                   >  2:5260777/1‑101 (MQ=255)
                                                                         gcTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTcc                              >  1:318510/1‑101 (MQ=255)
                                                                         gcTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTcc                              <  2:5477026/101‑1 (MQ=255)
                                                                          cTTGGGGAAGCGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTTAGAACGGCACGTCGTCCa                             <  2:589208/101‑1 (MQ=255)
                                                                          cTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                             <  1:1152250/101‑1 (MQ=255)
                                                                          cTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                             >  2:2137305/1‑101 (MQ=255)
                                                                           ttGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAg                            >  1:1554871/1‑101 (MQ=255)
                                                                            tGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGc                           >  2:1947203/1‑101 (MQ=255)
                                                                              gggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGccc                         <  2:4652972/101‑1 (MQ=255)
                                                                               ggAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCg                        <  2:2375225/101‑1 (MQ=255)
                                                                                gAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGa                       >  1:2144094/1‑101 (MQ=255)
                                                                                 aaaCGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAc                      <  1:6877326/101‑1 (MQ=255)
                                                                                 aaaCGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAc                      <  2:5686175/101‑1 (MQ=255)
                                                                                    cGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggc                   <  1:5261043/101‑1 (MQ=255)
                                                                                    cGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggc                   >  1:6415529/1‑101 (MQ=255)
                                                                                     ggTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggcg                  >  2:4790054/1‑101 (MQ=255)
                                                                                     ggTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggcg                  >  2:6812303/1‑101 (MQ=255)
                                                                                      gTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGCcggcgg                 >  2:6992846/1‑101 (MQ=255)
                                                                                        cTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGt               >  1:6308861/1‑101 (MQ=255)
                                                                                         ttG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCCCGTCGTCCAGCCCGACGGCGGGtt              <  2:3400191/101‑1 (MQ=255)
                                                                                           g.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTc            <  2:5057018/101‑1 (MQ=255)
                                                                                           g.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTc            >  1:4200267/1‑101 (MQ=255)
                                                                                             ccGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCa           <  1:1584556/101‑1 (MQ=255)
                                                                                             ccGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCa           <  1:6519352/101‑1 (MQ=255)
                                                                                             ccGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCa           <  1:3752896/101‑1 (MQ=255)
                                                                                               gcgGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAgg         <  2:2457331/101‑1 (MQ=255)
                                                                                               gcgGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAgg         >  1:1344100/1‑101 (MQ=255)
                                                                                                cgGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGa        >  1:5805797/1‑101 (MQ=255)
                                                                                                   cTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAcc     <  2:4200072/101‑1 (MQ=255)
                                                                                                    tGTTGCG.ATTCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAccc    >  1:5382209/1‑101 (MQ=255)
                                                                                                    tGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAccc    <  1:3866918/101‑1 (MQ=255)
                                                                                                    tGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGAAccc    >  1:975779/1‑101 (MQ=255)
                                                                                                     gTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCg   >  1:6715623/1‑101 (MQ=255)
                                                                                                      ttGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCgg  <  2:171117/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCG.CCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CCGCGGCTGTTGCG.ATGCGGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGG  >  NC_014034/1613910‑1614110

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG