Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,614,016 T→A 1.3% intergenic (+179/+48) rnd2 → / ← uvrD ribonuclease D/DNA helicase II

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,614,0160T→A 1.3% 3.0 ‑2.1 ‑0.7307intergenic (+179/+48)rnd2/uvrDribonuclease D/DNA helicase II
Reads supporting (aligned to +/- strand):  new base (0/4):  ref base (151/152):  total (151/156)
Fisher's exact test strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.07e-01

Only 100 of 320 total aligned reads displayed.

CCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCTTGA  >  NC_014034/1613916‑1614116
                                                                                                      |                                                                                                     
ctgccccccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGAt                                                                                                       <  1:6331297/95‑1 (MQ=255)
 cTGCGCCCGCCCCGCCATCGGCGGGCGCATTTGCGCCGCCGGGGGGGGGCGCCGGGCCTTGTTGTGGTTTGGGGAAAGGGCTTT.GC.GCGGCGGTTGCGGTg                                                                                                      >  1:785131/1‑101 (MQ=9)
 cTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATg                                                                                                      <  2:1834593/101‑1 (MQ=255)
    gccccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATgc.gg                                                                                                  <  2:2184014/99‑1 (MQ=255)
      cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATgc.gggc                                                                                                <  1:2418113/101‑1 (MQ=255)
      cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATgc.gggc                                                                                                <  1:1432633/101‑1 (MQ=255)
      cccgccccgcCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATgc.gggc                                                                                                <  2:774603/101‑1 (MQ=255)
       ccgccccgccATCGGCGGGCGCCATGGCGCCCCCCGGGGGGGCTGTGGGCCCTTGTTGGCCTTGGGGAACGGGACTTT.CG.ACGGCGGTTACGGTgg.ggggg                                                                                               >  2:4213384/1‑99 (MQ=255)
          ccccgccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCgggg                                                                                            <  1:4461360/101‑1 (MQ=255)
           cccgccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGa                                                                                           <  1:1941851/101‑1 (MQ=255)
            ccgccATCGGCGGGCGCAGTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGaa                                                                                          <  2:2701234/101‑1 (MQ=255)
             cgccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGCCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGaaa                                                                                         <  1:5018192/101‑1 (MQ=255)
               cccatggcgggcgCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTCCCGCGCGCTTGGGGAGGCGATCTTGTCC.CCGGCTTTGGTGGGGC.CGGCggggggag                                                                                        >  2:4378672/6‑97 (MQ=18)
               ccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTTTTTTGCGCTTTGGGGAACCGGCTTG.CC.GCGGCTGTTGCGGCGCGGGGCGGGGaagg                                                                                        >  2:4795828/1‑99 (MQ=18)
               ccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGGCGCAGGCCTTTGTTGCGCTTTGGGGAACGGTCTTG.CC.GCGGCTGTTGCGGTGC.GGGCGGGGGAAgg                                                                                       >  2:7081268/1‑101 (MQ=18)
               ccATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.CCGGCTGTTGCGATGC.TGGCGGGGAAAgg                                                                                       <  1:6273870/101‑1 (MQ=255)
                cATCGGCGGGCGCATTCGCGCCGCCGGGGGGGGGCGCAGGGCTTTTTTTCGCTTGGGGGAAAGGTCTTG.CC.GCGGCCGGTGCGGTGC.GGGGGGGGGAAggg                                                                                      >  2:1619019/1‑100 (MQ=17)
                  tCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAg                                                                                    >  2:653659/1‑101 (MQ=255)
                   gggcaggcgCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGt                                                                                   <  1:2225340/100‑1 (MQ=255)
                   cggcggGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGt                                                                                   <  2:5750713/101‑1 (MQ=255)
                      cgggcgCAGTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGcc                                                                                <  2:3578674/101‑1 (MQ=255)
                          cgcATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTc                                                                            >  1:344861/1‑101 (MQ=255)
                            cATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCt                                                                          >  2:3866755/1‑101 (MQ=255)
                              ttCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTTCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGa                                                                        <  2:6194904/101‑1 (MQ=255)
                                 gcgcCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATcc                                                                     <  1:4351039/101‑1 (MQ=255)
                                 gcgcCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATcc                                                                     <  1:3563254/101‑1 (MQ=255)
                                   gccgccGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGc                                                                   >  2:4969857/1‑101 (MQ=255)
                                    ccgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGcc                                                                  <  1:6942217/101‑1 (MQ=255)
                                     cgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                                                                 <  1:1776872/101‑1 (MQ=255)
                                     cgccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGccc                                                                 <  2:1142461/101‑1 (MQ=255)
                                      gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                                <  2:834243/101‑1 (MQ=255)
                                      gccgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCg                                                                >  1:5934710/1‑101 (MQ=255)
                                        cgGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGaa                                                              >  1:315519/1‑101 (MQ=255)
                                         ggggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAGAGGCAGTGCCCTTCCTGATCCGCCCGAAc                                                             >  1:4502289/1‑101 (MQ=255)
                                          gggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                                                            >  2:1337587/1‑101 (MQ=255)
                                          gggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                                                            <  1:2682927/101‑1 (MQ=255)
                                          gggCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAAcc                                                            <  2:3709033/101‑1 (MQ=255)
                                             cGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                         <  2:2635670/101‑1 (MQ=255)
                                             cGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                         <  2:6782203/101‑1 (MQ=255)
                                             cGGGACGCACGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCggg                                                         <  1:3284922/101‑1 (MQ=255)
                                              gggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCgggg                                                        >  1:3018929/1‑101 (MQ=255)
                                               ggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc                                                       >  2:2527650/1‑101 (MQ=255)
                                               ggACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGc                                                       >  1:1075198/1‑101 (MQ=255)
                                                 aCGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGTCCGAACCGGGGCTc                                                     >  1:54592/1‑101 (MQ=255)
                                                 aCGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTc                                                     >  2:4872672/1‑101 (MQ=255)
                                                 aCGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTc                                                     <  1:86345/101‑1 (MQ=255)
                                                   gCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg                                                   <  2:2259915/101‑1 (MQ=255)
                                                   gCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAg                                                   >  1:3939329/1‑101 (MQ=255)
                                                    cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                  >  2:5836551/1‑101 (MQ=255)
                                                    cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                  >  1:1635144/1‑101 (MQ=255)
                                                    cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                  >  2:6285186/1‑101 (MQ=255)
                                                    cAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGa                                                  >  1:5769358/1‑101 (MQ=255)
                                                     aGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa                                                 <  1:3178672/101‑1 (MQ=255)
                                                     aGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGaa                                                 >  1:589228/1‑101 (MQ=255)
                                                      ggCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc                                                >  2:4565582/1‑101 (MQ=255)
                                                      ggCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAc                                                <  2:5356654/101‑1 (MQ=255)
                                                       gCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg                                               >  2:5426121/1‑101 (MQ=255)
                                                       gCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACg                                               <  2:3184118/101‑1 (MQ=255)
                                                        ccTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACgg                                              >  2:2971770/1‑101 (MQ=255)
                                                         cTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGc                                             >  1:5189375/1‑101 (MQ=255)
                                                          tttGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCa                                            >  1:1562855/1‑101 (MQ=255)
                                                           ttgttgCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAc                                           <  1:965543/101‑1 (MQ=255)
                                                            tgttgCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcg                                          >  2:3201458/1‑101 (MQ=255)
                                                             gttgCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAAAGGCAcgt                                         >  2:5112741/1‑101 (MQ=255)
                                                             gttgCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTAATCCGCCCGAACCGGGGCTCAGAACGGCAcgt                                         >  2:5260777/1‑101 (MQ=255)
                                                               tgCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtcg                                       >  2:4730237/1‑101 (MQ=255)
                                                               tgCCCTTGGGGGAACGGGCTTG.CCGGCGGTGGTGGCG.TGG.GGGGGGGGGAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCAcgtcg                                       <  1:4736410/101‑1 (MQ=16)
                                                                   cTTGGGGAAGCGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTTAGAACGGCACGTCGTCCa                                   <  2:589208/101‑1 (MQ=255)
                                                                   cTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                                   >  2:2137305/1‑101 (MQ=255)
                                                                   cTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                                   <  2:2008701/101‑1 (MQ=255)
                                                                   cTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                                   <  1:1152250/101‑1 (MQ=255)
                                                                   cTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCa                                   <  1:1533058/101‑1 (MQ=255)
                                                                     tGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGc                                 >  2:2489000/1‑101 (MQ=255)
                                                                      ggggAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGcc                                <  1:2738089/101‑1 (MQ=255)
                                                                        ggAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCg                              <  2:6319488/101‑1 (MQ=255)
                                                                        ggAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCg                              <  2:2375225/101‑1 (MQ=255)
                                                                        ggAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCg                              <  1:4970109/101‑1 (MQ=255)
                                                                           aaCGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcg                           >  1:2560091/1‑101 (MQ=255)
                                                                             cGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggc                         <  1:1376331/101‑1 (MQ=255)
                                                                             cGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggc                         <  1:5261043/101‑1 (MQ=255)
                                                                               gTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGAcggcgg                       >  2:4097130/1‑101 (MQ=255)
                                                                                tCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCggg                      >  1:6456406/1‑101 (MQ=255)
                                                                                 cTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGt                     >  2:918337/1‑101 (MQ=255)
                                                                                  ttG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCCCGTCGTCCAGCCCGACGGCGGGtt                    <  2:3400191/101‑1 (MQ=255)
                                                                                  ttG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGtt                    <  1:1337638/101‑1 (MQ=255)
                                                                                   tG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGttt                   >  1:5585062/1‑101 (MQ=255)
                                                                                    g.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTc                  <  2:5057018/101‑1 (MQ=255)
                                                                                      cc.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCa                 <  2:572529/101‑1 (MQ=255)
                                                                                       c.gcgGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAg                <  1:6636453/101‑1 (MQ=255)
                                                                                          cgGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGa              >  1:5805797/1‑101 (MQ=255)
                                                                                          cgGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGa              <  1:2137385/101‑1 (MQ=255)
                                                                                           ggCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGaa             >  2:5552293/1‑101 (MQ=255)
                                                                                             cTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAcc           <  1:3767208/101‑1 (MQ=255)
                                                                                              tGTTGCGATTC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAAccc          >  1:5382209/1‑101 (MQ=255)
                                                                                              tGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGAAccc          >  1:6809412/1‑101 (MQ=255)
                                                                                                ttGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCgg        <  2:171117/101‑1 (MQ=255)
                                                                                                 tGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGGTTCAGGAACCCGGc       >  1:4051242/1‑101 (MQ=255)
                                                                                                   cGATCC.CGGCGGGGAAACGCAGTGCCCGTCCTGATCCGCGCGAACCCGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCt     <  2:6930761/101‑1 (MQ=255)
                                                                                                    gATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGCTTCAGGAACCCGGCCtt    >  2:5643319/1‑101 (MQ=255)
                                                                                                     aTGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCTTg   <  1:7125227/101‑1 (MQ=255)
                                                                                                      |                                                                                                     
CCTGCGCCCGCCCCGCCATCGGCGGGCGCATTCGCGCCGCCGGGGCGGGACGCAGGCCTTTGTTGCGCTTGGGGAAACGGTCTTG.CC.GCGGCTGTTGCGATGC.GGGCGGGGAAAGGCAGTGCCCTTCCTGATCCGCCCGAACCGGGGCTCAGAACGGCACGTCGTCCAGCCCGACGGCGGGTTTCAGGAACCCGGCCTTGA  >  NC_014034/1613916‑1614116

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG