Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,668,401	G→C	100%	intergenic (‑66/+16)	ilvH ← / ← ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,668,401	0	G→C	100.0%	155.5	43	intergenic (‑66/+16)	ilvH/ilvI	acetolactate synthase small subunit/acetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (43/0):  ref base (0/0):  total (43/0)

CCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGGA  >  NC_014034/1668310‑1668491                                                                                            |                                                                                           ccTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGcc                                                                                 >  2:5126375/1‑101 (MQ=255) ccTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGcc                                                                                 >  2:3356546/1‑101 (MQ=255)  cTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCg                                                                                >  1:6651678/1‑101 (MQ=255)      cTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGctct                                                                            >  2:5751660/1‑101 (MQ=255)             ttCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCa                                                                     >  2:6032382/1‑101 (MQ=255)                   gCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCgcgg                                                               >  2:4782335/1‑101 (MQ=255)                         aTTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCACCGCGGcgccgc                                                         >  2:6879042/1‑101 (MQ=25)                           tGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGcgccgccg                                                       >  1:6968647/1‑101 (MQ=255)                                               tGATTTTTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGc                                   >  1:4398874/1‑101 (MQ=37)                                               tGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGc                                   >  1:59887/1‑101 (MQ=255)                                                 aTTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGCAGGCCTCGGCATCGGCCg                                 >  1:1745148/1‑101 (MQ=25)                                                  tttATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCga                                >  1:1142446/1‑101 (MQ=255)                                                  tttATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCga                                >  1:3070161/1‑101 (MQ=255)                                                    tATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCgaga                              >  1:1217998/1‑101 (MQ=255)                                                     aTCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGagag                             >  2:3030619/1‑101 (MQ=255)                                                       cTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGagca                           >  1:1085344/1‑101 (MQ=255)                                                            cGGAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAGGGACTCGGCATCGGCCGAGAGCAGCAtt                      >  1:2077537/1‑101 (MQ=37)                                                             ggAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCAttt                     >  1:1793512/1‑101 (MQ=255)                                                              gAAATATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTc                    >  1:2959292/1‑101 (MQ=255)                                                                aaTATCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGt                  >  1:4114180/1‑101 (MQ=255)                                                                  tatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTa                >  2:6382347/1‑101 (MQ=255)                                                                  tatCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTa                >  2:5061799/1‑101 (MQ=255)                                                                   atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt               >  1:6048553/1‑101 (MQ=255)                                                                   atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt               >  2:1100976/1‑101 (MQ=255)                                                                   atCCCGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTAt               >  2:3908960/1‑101 (MQ=255)                                                                     cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc             >  1:4871045/1‑101 (MQ=255)                                                                     cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc             >  1:1186048/1‑101 (MQ=255)                                                                     cccGGGGGGTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGAGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGc             >  1:3232504/1‑101 (MQ=38)                                                                        ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc          >  2:903462/1‑101 (MQ=255)                                                                        ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc          >  1:2376902/1‑101 (MQ=255)                                                                        ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc          >  2:3727599/1‑101 (MQ=255)                                                                        ggggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGc          >  2:1007670/1‑101 (MQ=255)                                                                         gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt         >  2:4633441/1‑101 (MQ=255)                                                                         gggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCt         >  1:4001320/1‑101 (MQ=255)                                                                          ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt        >  2:1173509/1‑101 (MQ=255)                                                                          ggggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCtt        >  1:3059954/1‑101 (MQ=255)                                                                           gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg       >  2:219606/1‑101 (MQ=255)                                                                           gggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTg       >  2:7117068/1‑101 (MQ=255)                                                                            ggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGc      >  1:3615311/1‑101 (MQ=255)                                                                            ggTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGc      >  1:5289737/1‑101 (MQ=255)                                                                             gTCCGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGcc     >  1:2837136/1‑101 (MQ=255)                                                                               ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg   >  2:606672/1‑101 (MQ=255)                                                                               ccGGGGGGCA..GCGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCgg   >  1:4423794/1‑101 (MQ=255)                                                                                  gaacgcgatcggCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGGa  <  2:4436806/91‑1 (MQ=255)                                                                                            |                                                                                           CCTTTCTTGATGTTCAGAGCGGCCATTGCCCTCTCCCTTCCGATACTGATTTATCTTGCCGGAAATATCCCGGGGGGTCCGGGGGGCAAGGGGCCCCCCGGCCGCTCTCACACCAGCGCGGCGCCGCCGCGGAAGGCCTCGGCATCGGCCGAGAGCAGCATTTCGTTATGCGGCTTGCCGGA  >  NC_014034/1668310‑1668491 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG