Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,733 C→T 100% S477N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,7330C→T100.0% 1250.7 321S477N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (152/168):  ref base (0/0):  total (153/168)

Only 100 of 336 total aligned reads displayed.

CCAGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830
                                                                                                  |                                                                                                 
  aGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGtt                                                                                               <  2:6571829/101‑1 (MQ=255)
   gTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTc                                                                                              <  1:5028626/101‑1 (MQ=255)
     ttCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCNAGCCGCTCGTTGTTCAg                                                                                            >  2:147297/1‑101 (MQ=255)
     ttCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAg                                                                                            <  1:4676890/101‑1 (MQ=255)
      tCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAgg                                                                                           >  1:3645086/1‑101 (MQ=255)
      tCACGAAATCGGGCAGGCTTCCCGACCAGCTTGGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAgg                                                                                           <  2:1186346/101‑1 (MQ=255)
           aaaTCGGGCAGGCTTTCCGCCCAGCTTTCCGAATACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGaa                                                                                      <  1:1047779/101‑1 (MQ=255)
           aaaTCGGGCAGGCTTTCCGACCAGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGaa                                                                                      <  1:5134982/101‑1 (MQ=255)
             aTCGGGCAGGCTTCCCGACCAGCTTTGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACt                                                                                    <  1:2490902/101‑1 (MQ=255)
               cGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGc                                                                                  >  2:1035270/1‑101 (MQ=255)
               cGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGc                                                                                  >  1:5828662/1‑101 (MQ=255)
                gggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCt                                                                                 >  2:6036360/1‑101 (MQ=255)
                gggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCt                                                                                 >  1:2411984/1‑101 (MQ=255)
                gggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCt                                                                                 >  2:2094789/1‑101 (MQ=255)
                gggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATAAACTGCt                                                                                 >  1:1229434/1‑101 (MQ=255)
                gggCAGGCTTCCCGACCGCCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCt                                                                                 <  2:2124961/101‑1 (MQ=255)
                 ggCAGGTTTTCCGCCCGGCTTGGCGAATACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                <  2:6452544/101‑1 (MQ=255)
                 ggCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCtt                                                                                >  1:4154643/1‑101 (MQ=255)
                  gCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTg                                                                               <  2:5905034/101‑1 (MQ=255)
                    aGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGTCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAc                                                                             <  2:2811747/101‑1 (MQ=255)
                    aGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGAc                                                                             >  1:146697/1‑101 (MQ=255)
                       cTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgg                                                                          >  2:4204455/1‑101 (MQ=255)
                        tttCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCggg                                                                         >  1:99052/1‑101 (MQ=255)
                         ttCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgggg                                                                        >  2:167233/1‑101 (MQ=255)
                           ccGCCCAGCTTTGCAAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTg                                                                      <  2:3850201/101‑1 (MQ=255)
                           ccGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCgggggg                                                                      >  2:6897168/1‑99 (MQ=255)
                            cGACCAGCTTTGCGAATAACCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGc                                                                     <  2:1878301/101‑1 (MQ=255)
                             gACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCg                                                                    >  1:2640801/1‑101 (MQ=255)
                              aCCAGCTTTGGAAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCgg                                                                   <  2:4687256/101‑1 (MQ=255)
                              aCCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCgg                                                                   >  2:3753124/1‑101 (MQ=255)
                              aCCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCgg                                                                   >  1:7070233/1‑101 (MQ=255)
                                cAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGaa                                                                 >  1:4813448/1‑101 (MQ=255)
                                 aGCTTTGCGAATACCGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAAc                                                                <  2:2937778/101‑1 (MQ=255)
                                 aGCTTTGCGAACACCCCCCGCCAGGCAGCGGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAAc                                                                <  2:1405726/101‑1 (MQ=255)
                                 aGCTTGGCGAATACCCCCCGCCATCCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAAc                                                                <  2:7036410/101‑1 (MQ=255)
                                  gCTTGGCGAATACCCCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACt                                                               <  1:2920576/101‑1 (MQ=255)
                                   cTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTg                                                              >  2:3686284/1‑101 (MQ=255)
                                    tttGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             >  1:2332403/1‑101 (MQ=255)
                                    tttGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGc                                                             >  2:4440862/1‑101 (MQ=255)
                                      tGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGt                                                           >  2:6846218/1‑101 (MQ=255)
                                       gCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTc                                                          >  2:1285224/1‑101 (MQ=255)
                                         gAATAACGCCCGCCATGCTGCAGGTCCTGCCACTGCCGCACCATGCCCAGTCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCCGAACTGCGTCGc                                                        >  2:4543353/1‑101 (MQ=255)
                                         gAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGc                                                        >  2:531703/1‑101 (MQ=255)
                                          aaTAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGcc                                                       >  1:3065960/1‑101 (MQ=255)
                                           gatctgcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCg                                                      >  2:568241/6‑101 (MQ=255)
                                           atcacccccgccATGAAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCg                                                      <  2:2588627/95‑1 (MQ=255)
                                            taccgcccgccATGCAGCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     <  2:4941059/98‑1 (MQ=255)
                                            tAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGt                                                     >  2:7092806/1‑101 (MQ=255)
                                             accccccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTg                                                    <  1:3973450/97‑1 (MQ=255)
                                                gcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 >  1:804477/1‑101 (MQ=255)
                                                gcccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 >  1:4459617/1‑101 (MQ=255)
                                                ccccgccTTGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGccc                                                 <  2:3913441/100‑1 (MQ=255)
                                                 cccgccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCa                                                <  1:4257011/101‑1 (MQ=255)
                                                  ccgccATGCAGCGGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAt                                               <  1:3210072/101‑1 (MQ=255)
                                                    gccATGCAGCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             <  1:5359365/101‑1 (MQ=255)
                                                    cctaTGCAGCAGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCAttt                                             <  2:307334/98‑1 (MQ=255)
                                                     ccATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGAGGAACTACGGGACCGGGGTGCGAGACTGCGTCGCCGTGctcgttgc                                            >  2:2281503/1‑94 (MQ=255)
                                                      cATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTcc                                           >  1:474460/1‑101 (MQ=255)
                                                         gcagcaGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGc                                        >  2:362934/1‑101 (MQ=255)
                                                         gcagcaGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGc                                        >  1:6149516/1‑101 (MQ=255)
                                                         gcagcaGGCCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGc                                        <  2:5003688/101‑1 (MQ=255)
                                                          cagcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCa                                       >  1:1430854/1‑101 (MQ=255)
                                                           agcagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCAt                                      <  1:2188259/101‑1 (MQ=255)
                                                             cagGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGt                                    >  1:5928858/1‑101 (MQ=255)
                                                              agGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGtt                                   >  2:6523647/1‑101 (MQ=255)
                                                              agGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGtt                                   >  1:6241957/1‑101 (MQ=255)
                                                               ggcccTGCCCCTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtc                                  <  2:2411890/98‑1 (MQ=255)
                                                               ggTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtc                                  >  2:2538128/1‑101 (MQ=255)
                                                                gTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtca                                 >  1:1948173/1‑101 (MQ=255)
                                                                 cccTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcat                                <  1:3864506/100‑1 (MQ=255)
                                                                   cTGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTtcatca                              <  1:6563970/101‑1 (MQ=255)
                                                                    tGCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAg                             <  1:3463820/101‑1 (MQ=255)
                                                                     gCCACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGc                            >  1:202770/1‑101 (MQ=255)
                                                                       cACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCa                          <  2:1942766/101‑1 (MQ=255)
                                                                       cACTGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCa                          <  1:3144166/101‑1 (MQ=255)
                                                                          tGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGa                       >  1:689775/1‑101 (MQ=255)
                                                                          tGCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGa                       <  1:5725304/101‑1 (MQ=255)
                                                                           gCCGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGAc                      <  2:2366241/101‑1 (MQ=255)
                                                                            ccGCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGCGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACg                     >  1:7135336/1‑101 (MQ=255)
                                                                              gCACCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGcc                   >  1:3695348/1‑101 (MQ=255)
                                                                                cccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTc                 <  1:1653355/100‑1 (MQ=255)
                                                                                aCCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTc                 <  2:3188006/101‑1 (MQ=255)
                                                                                aCCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTc                 <  1:6396264/101‑1 (MQ=255)
                                                                                aCCATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTc                 <  1:6681985/101‑1 (MQ=255)
                                                                                 ccATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGCCGCCTCa                >  2:5764114/1‑101 (MQ=255)
                                                                                  cATGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAc               <  2:149488/101‑1 (MQ=255)
                                                                                   aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAcc              <  1:2837597/101‑1 (MQ=255)
                                                                                   aTGCCCAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCAcc              >  2:525025/1‑101 (MQ=255)
                                                                                      cccAGCCGCTCGTTGTTCAGTATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGc           <  1:6846572/101‑1 (MQ=255)
                                                                                      cccAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGc           <  1:5279502/101‑1 (MQ=255)
                                                                                       ccAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCg          >  1:1477115/1‑101 (MQ=255)
                                                                                        cAGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGa         >  2:6242614/1‑101 (MQ=255)
                                                                                         cgCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        <  2:3345591/100‑1 (MQ=255)
                                                                                         aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        >  1:1151348/1‑101 (MQ=255)
                                                                                         aGCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGAt        >  2:3487575/1‑101 (MQ=255)
                                                                                          gCCGCTCGTTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATg       <  2:543808/101‑1 (MQ=255)
                                                                                             gctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTg    >  2:5571848/1‑101 (MQ=255)
                                                                                             gctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTg    >  2:1540137/1‑101 (MQ=255)
                                                                                             gctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTg    <  1:6346811/101‑1 (MQ=255)
                                                                                              ctcgttGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGa   >  2:6797276/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCAGTTTCACGAAATCGGGCAGGCTTTCCGACCAGCTTTGCGAATAACGCCCGCCATGCAGCAGGTCCTGCCACTGCCGCACCATGCCCAGCCGCTCGCTGTTCAGGATGAACTGCTTGACCGGGGTGCGGAACTGCGTCGCCGTGCCCATTTCCTGCATGTTCATCAGCCAGGACGCCTCACCGGCGATGTTGAT  >  NC_014034/1668635‑1668830

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG