Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,994 C→T 100% S390N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,9940C→T100.0% 1077.3 281S390N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (171/108):  ref base (0/0):  total (173/108)

Only 100 of 323 total aligned reads displayed.

CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCA  >  NC_014034/1668896‑1669091
                                                                                                  |                                                                                                 
 cAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgc                                                                                                >  1:5215405/1‑101 (MQ=255)
 cAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTAGGTGGTGATGTAGCGGTTgc                                                                                                >  1:4351945/1‑101 (MQ=255)
  aGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgcg                                                                                               >  2:5409279/1‑101 (MQ=255)
  aGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgcg                                                                                               >  1:375614/1‑101 (MQ=255)
  aGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgcg                                                                                               >  1:1630580/1‑101 (MQ=255)
  aGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgcg                                                                                               >  1:1007591/1‑101 (MQ=255)
   gCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTgcgc                                                                                              >  1:3841952/1‑101 (MQ=255)
   gCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCCGGCCGACTTCGGTGGTGATGTAGCGGTTgcgc                                                                                              >  1:3405919/1‑101 (MQ=255)
     ccTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGccc                                                                                            <  1:5369210/101‑1 (MQ=255)
     ccTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGccc                                                                                            >  1:1233944/1‑101 (MQ=255)
      cTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGcccc                                                                                           <  2:4083139/101‑1 (MQ=255)
      cTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGcccc                                                                                           >  2:2165592/1‑101 (MQ=255)
         cGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTg                                                                                        <  2:5848148/101‑1 (MQ=255)
                  tCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGcc                                                                               <  1:251197/101‑1 (MQ=255)
                   ccAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATATGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCt                                                                              >  1:3287296/1‑101 (MQ=255)
                    cAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtc                                                                             <  2:6836246/101‑2 (MQ=255)
                    cAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtc                                                                             <  2:299045/101‑2 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                            >  1:1948367/1‑99 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                            >  1:5907747/1‑99 (MQ=255)
                     aGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                            <  2:3952408/101‑3 (MQ=255)
                        ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGc                                                                         <  2:4358219/101‑1 (MQ=255)
                          ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCg                                                                       <  2:5322710/101‑1 (MQ=255)
                              gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                   <  1:2112894/101‑1 (MQ=255)
                               cgcCTCGAAGCCCAGATATGGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGAGGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcag                                                                  <  1:3047790/101‑3 (MQ=255)
                                 ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgc                                                                >  1:1887789/1‑101 (MQ=255)
                                   tCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcgc                                                              >  1:6537072/1‑101 (MQ=255)
                                    cGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCa                                                             >  2:1833398/1‑101 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                            <  2:33490/101‑1 (MQ=255)
                                      aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                           <  2:4454501/101‑1 (MQ=255)
                                         cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                        >  1:155133/1‑101 (MQ=255)
                                         cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                        >  1:72552/1‑101 (MQ=255)
                                          ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                       >  2:3089522/1‑101 (MQ=255)
                                          ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                       >  1:5551909/1‑101 (MQ=255)
                                           cAGATATTGCGTCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                      >  2:2840503/1‑101 (MQ=255)
                                           cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                      >  2:1962919/1‑101 (MQ=255)
                                            aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                     >  1:4850494/1‑101 (MQ=255)
                                             gATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGt                                                    >  1:5088867/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                   <  2:3836992/101‑1 (MQ=255)
                                               tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                  <  2:3211456/101‑1 (MQ=255)
                                               tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                  >  1:214141/1‑101 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                 >  2:614707/1‑101 (MQ=255)
                                                 ttGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                                <  1:7034731/101‑2 (MQ=255)
                                                 ttGCCCCGCCCACCACCGAAGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                                <  2:5034679/101‑2 (MQ=255)
                                                 tgtagacggccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                                >  2:6764333/10‑100 (MQ=255)
                                                  tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                               >  2:2535071/1‑99 (MQ=255)
                                                   gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                              <  2:4154343/101‑1 (MQ=255)
                                                   gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                              >  1:2378281/1‑101 (MQ=255)
                                                    cgccgcCCAAATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGa                                             <  1:2200224/101‑1 (MQ=255)
                                                       cgccCATATCTGATTCTGGTCGACTCCGGTGGTGATGTAGGGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCTCATATTGCGGTTTGATGATGc                                          <  1:2787512/101‑1 (MQ=255)
                                                       cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                          >  2:1256804/1‑101 (MQ=255)
                                                         cccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                        <  1:1677823/101‑1 (MQ=255)
                                                         cccACATCTGATGCTGGCCGACTTCGGGGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTg                                        >  2:245850/1‑101 (MQ=255)
                                                           cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                      >  1:3148486/1‑101 (MQ=255)
                                                           cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                      <  2:135960/101‑1 (MQ=255)
                                                           cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                      >  2:1548368/1‑101 (MQ=255)
                                                           cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                      >  2:6294556/1‑101 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCTCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                    <  1:6436406/101‑1 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                    <  1:3640142/101‑1 (MQ=255)
                                                             caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                    >  2:1737055/1‑101 (MQ=255)
                                                               tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                  >  1:1393423/1‑101 (MQ=255)
                                                                cTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                                 <  2:6911049/101‑1 (MQ=255)
                                                                 tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                >  1:5718094/1‑101 (MQ=255)
                                                                 tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                <  1:1833462/101‑1 (MQ=255)
                                                                  gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                               >  2:5277984/1‑101 (MQ=255)
                                                                  gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                               <  1:614727/101‑1 (MQ=255)
                                                                   aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCgg                              >  2:4701313/1‑101 (MQ=255)
                                                                   aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCACATATTGCGGTTTGATGATGCTGTCCGAGCCgg                              >  1:4294366/1‑101 (MQ=255)
                                                                     gCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTTGTCAGGGCCTCCAGCCGCTCCAGCGCATANNNCNGTTTGATGATGCTGTCCGAGCCgggg                            >  1:458978/1‑99 (MQ=255)
                                                                       tGGCCGATTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                          <  2:4556046/101‑1 (MQ=255)
                                                                       tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                          <  1:89262/101‑1 (MQ=255)
                                                                        ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                         <  1:6074157/101‑1 (MQ=255)
                                                                        ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                         <  2:4193899/101‑1 (MQ=255)
                                                                         gCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                        <  1:5437104/101‑1 (MQ=255)
                                                                          cctccTTGGGTGGTGTTGTAGCGTTTGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                       <  2:5541694/97‑1 (MQ=255)
                                                                          ccGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCAGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGc                       <  1:6742798/101‑1 (MQ=255)
                                                                           cGATTTTGGTGGTGATGTAGCGTTTGCGCCCCTTGTTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                      <  2:6380781/101‑1 (MQ=255)
                                                                             aCTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                    >  2:2048912/1‑101 (MQ=255)
                                                                              cTTGGGTGGTGATGTAGCGGTTGCGCCCCTGGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                   <  1:29430/101‑1 (MQ=255)
                                                                              cTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                   >  2:6024439/1‑101 (MQ=255)
                                                                              cTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                   <  1:2668073/101‑1 (MQ=255)
                                                                               ttCGGTGGGGATGTAGCGGTTGCGCCCCTTGGTCAGGTCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                  <  1:2840605/101‑1 (MQ=255)
                                                                                tCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                 >  2:1147058/1‑101 (MQ=255)
                                                                                tCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                 >  1:4188287/1‑101 (MQ=255)
                                                                                 cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                >  1:6781884/1‑101 (MQ=255)
                                                                                  ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc               >  2:3678662/1‑101 (MQ=255)
                                                                                  ggtggtGAGGTAGCGGTGGCGCCCCTGGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc               <  2:804453/101‑1 (MQ=255)
                                                                                   gtggtgGTGTGGCGGTGGCGCCCCTTGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg              <  1:4225674/101‑1 (MQ=255)
                                                                                   gtggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg              >  2:23080/1‑101 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg             >  2:5477422/1‑101 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg             >  2:3518795/1‑101 (MQ=255)
                                                                                     ggtgATTTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa            <  1:996622/101‑1 (MQ=255)
                                                                                       tgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtc          >  1:3968697/1‑101 (MQ=255)
                                                                                        gATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct         >  1:5688452/1‑101 (MQ=255)
                                                                                        gATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGGGTAGGCGAGGCAGCGGactt         >  2:5716739/1‑98 (MQ=255)
                                                                                         gtGTGGCGGTTGCCCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtctt        <  2:6525545/100‑1 (MQ=255)
                                                                                          tGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc       >  1:4057552/1‑101 (MQ=255)
                                                                                           gTAGGGGTTGCGCCCCTTGGTCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct      <  1:6245274/101‑1 (MQ=255)
                                                                                           gTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtattct      >  2:273878/1‑101 (MQ=255)
                                                                                            tAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt     >  2:5952320/1‑101 (MQ=255)
                                                                                             aGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCTGTGCCGGTGTAGGCGAGGCAGCGGGTCctcttc    >  2:2627162/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCA  >  NC_014034/1668896‑1669091

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG