Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,669,016 T→C 100% K383E (AAG→GAG)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,669,0160T→C100.0% 1100.6 289K383E (AAG→GAG) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (165/124):  ref base (0/0):  total (165/124)

Only 100 of 322 total aligned reads displayed.

GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGG.TCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668918‑1669113
                                                                                                   |                                                                                                 
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGc                                                                                               >  2:6818062/1‑101 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGc                                                                                               <  1:439458/101‑1 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGc                                                                                               <  2:4358219/101‑1 (MQ=255)
  ggTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGGTGCGGCCCTTGG.TCAGGGCCTCCAGc                                                                                               >  2:2234360/1‑101 (MQ=255)
    ttCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCg                                                                                             <  2:5322710/101‑1 (MQ=255)
          gcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAg                                                                                       <  1:3047789/101‑1 (MQ=255)
           ccTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAgc                                                                                      >  1:1887789/1‑101 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  <  2:33490/101‑1 (MQ=255)
               gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCat                                                                                  >  1:7064612/1‑101 (MQ=255)
                aaGCCCAGATTCTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 <  2:6921155/101‑1 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 <  2:4454501/101‑1 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:2137463/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  1:6565264/1‑101 (MQ=255)
                aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCata                                                                                 >  2:2840108/1‑101 (MQ=255)
                  gCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                                               >  2:3562285/1‑101 (MQ=255)
                   cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                              >  1:72552/1‑101 (MQ=255)
                   cccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTg                                                                              >  1:155133/1‑101 (MQ=255)
                    ccAGATATTGCGCCGCCCCCATCTGCTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                             <  1:2279455/101‑1 (MQ=255)
                    ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                                             >  2:3089522/1‑101 (MQ=255)
                      aGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCgg                                                                           >  1:5132577/1‑101 (MQ=255)
                       gATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGt                                                                          >  1:3950964/1‑101 (MQ=255)
                        atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                                         >  2:29429/1‑101 (MQ=255)
                        atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                                         >  1:2026221/1‑101 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  2:1125380/101‑1 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  2:3211456/101‑1 (MQ=255)
                         tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                                        <  1:1651357/101‑1 (MQ=255)
                          atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       <  2:2483679/101‑1 (MQ=255)
                          atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       <  2:6356476/101‑1 (MQ=255)
                          atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                                       >  1:4617370/1‑101 (MQ=255)
                           ttGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                                                      >  1:2799303/1‑100 (MQ=255)
                            tGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTgat                                                                     <  1:6891635/101‑3 (MQ=255)
                             gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                                                    <  1:106099/101‑1 (MQ=255)
                             gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                                                    >  1:2378281/1‑101 (MQ=255)
                               gccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGAt                                                                  <  2:832586/101‑1 (MQ=255)
                                    ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                                             >  2:120259/1‑101 (MQ=255)
                                     cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                                            >  2:1548368/1‑101 (MQ=255)
                                     cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                                            <  1:953990/101‑1 (MQ=255)
                                     cacaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTc                                                            <  1:492499/101‑1 (MQ=255)
                                       caTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCg                                                          >  2:1737055/1‑101 (MQ=255)
                                        aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                                         <  1:5758571/101‑1 (MQ=255)
                                         tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                                        >  1:5946791/1‑101 (MQ=255)
                                         tCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAg                                                        <  1:5427137/101‑1 (MQ=255)
                                          cTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAga                                                       >  2:5426865/1‑100 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      <  2:6614209/101‑1 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      <  1:1833462/101‑1 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      <  1:276824/101‑1 (MQ=255)
                                           tGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                                                      <  1:2924817/101‑1 (MQ=255)
                                            gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                     <  2:612940/101‑1 (MQ=255)
                                            gATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                                                     >  1:3236497/1‑101 (MQ=255)
                                             aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCgg                                                    >  1:6361396/1‑101 (MQ=255)
                                             aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCCTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCgg                                                    >  2:3785505/1‑101 (MQ=255)
                                              tGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCggg                                                   >  2:5646896/1‑100 (MQ=255)
                                                 tGGCCGATTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                                                <  2:4556046/101‑1 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                               <  1:6074157/101‑1 (MQ=255)
                                                  ggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAg                                               <  2:4193899/101‑1 (MQ=255)
                                                     cGATTTTGGTGGTGATGTAGCGTTTGCGCCCCTTGT.TCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            <  2:6380781/101‑1 (MQ=255)
                                                     cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                                            <  2:1808828/101‑1 (MQ=255)
                                                      gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                                           >  2:395268/1‑101 (MQ=255)
                                                       gcTTCGGTGGTGAGGTAGCGGTTGCGCCCCTTGG.TCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                                          <  2:4139639/100‑1 (MQ=255)
                                                        cTTGGGGGGTGATGTAGCGGTTGC.CCCCTTGGTTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAgg                                         <  2:600938/101‑1 (MQ=255)
                                                         ttCGGTGGTGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                                        >  1:1974154/1‑101 (MQ=255)
                                                         ttCGGTGGGGATGTAGCGGTTGCGCCCCTTGG.TCAGGTCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGc                                        <  1:2840605/101‑1 (MQ=255)
                                                          tCGGGGGTGAGTTAGCGGTTGCGCCCCTTGG.TCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCa                                       <  1:147300/101‑1 (MQ=255)
                                                           cGGTGGTGGTGTAGCGGTTGCGCCCCTTGG.TCAGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg                                      <  1:3073603/101‑1 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGG.TCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     <  1:2555161/101‑1 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCNGGTGTAGGCGAGGCAgg                                     >  2:1969470/1‑100 (MQ=255)
                                                            ggtggtGATGTAGCGGTTGCGCCCCTGGG.TCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGc                                     <  1:6770394/101‑1 (MQ=255)
                                                             gtggtgGTGTGGCGGTGGCGCCCCTTGG.TCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg                                    <  1:4225674/101‑1 (MQ=255)
                                                             gtggtgATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg                                    >  1:5692163/1‑101 (MQ=255)
                                                               ggtgATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                  >  2:3423541/1‑101 (MQ=255)
                                                               ggtgATGTAGCGGTTGCGCCCCTGGG.CCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGa                                  <  1:4300247/101‑1 (MQ=255)
                                                                gtgtggTAGCGGTTGCGCCCCTGGG.TCGGGGCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 <  2:2137383/96‑1 (MQ=255)
                                                                gtgATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCGCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt                                 >  1:3699734/1‑101 (MQ=255)
                                                                 tgATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtc                                >  2:4830106/1‑101 (MQ=255)
                                                                  gATGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtct                               >  1:6203906/1‑101 (MQ=255)
                                                                   aTGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtctt                              >  1:5306590/1‑101 (MQ=255)
                                                                    tgtaggtgtggcgcCCCTGGG.TCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                             <  2:1375953/91‑1 (MQ=255)
                                                                    tGTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc                             >  1:4057552/1‑101 (MQ=255)
                                                                     gTAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtattct                            >  2:273878/1‑101 (MQ=255)
                                                                      tAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGTtcttcag                           >  2:5272441/1‑99 (MQ=255)
                                                                      tAGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATcttctt                           >  2:5952320/1‑101 (MQ=255)
                                                                       ggcggtggcgcCCTTTGG.TCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCttcttc                          <  2:1948295/94‑1 (MQ=255)
                                                                       aGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCTGTGCCGGTGTAGGCGAGGCAGCGGGTCctcttc                          >  2:2627162/1‑101 (MQ=255)
                                                                       aGCGGTTGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttctag                          >  1:3584972/1‑99 (MQ=255)
                                                                        gCGGTTGCGCCCCTTGG.TCGGGGCCCCCGGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTcc                         <  1:5244784/101‑1 (MQ=255)
                                                                           gttGCGCCCCTTGG.TCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCAct                      <  1:1380984/99‑1 (MQ=255)
                                                                            ttGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActc                     >  2:581602/2‑101 (MQ=255)
                                                                             tGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  2:2400137/1‑101 (MQ=255)
                                                                             tGCGCCCCTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcc                    >  2:5490767/1‑101 (MQ=255)
                                                                              gccccccTTGC.TCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGTTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCActcct                   <  1:1256852/98‑1 (MQ=255)
                                                                                   ccTTGG.TCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATc              <  1:5497206/101‑1 (MQ=255)
                                                                                   ccTTGG.GCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATc              <  1:3423665/101‑1 (MQ=255)
                                                                                   acTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCCGCGGATCTTCTTCCACTCCTCGCTc              >  2:7113071/2‑101 (MQ=255)
                                                                                    cTTGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt             >  1:2169319/1‑101 (MQ=255)
                                                                                    cTTGG.CCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCt             <  2:4654766/101‑1 (MQ=255)
                                                                                     ttGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt            >  1:6457628/1‑101 (MQ=255)
                                                                                     tggg.TCAGGCCCCCCAGCCGCTCCAGCGCATTTTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCtt            <  2:6180738/99‑1 (MQ=255)
                                                                                      tGG.TCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           >  2:5048283/1‑101 (MQ=255)
                                                                                      tGG.TCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGGCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTg           >  2:1278998/1‑101 (MQ=255)
                                                                                                ccTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAt  <  2:1324859/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGG.TCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCCACTCCTCGATCTTGCCCCACCAT  >  NC_014034/1668918‑1669113

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG