Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,179 A→C 6.5% intergenic (‑107/+118) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1790A→C 6.5% 53.6 ‑0.6 ‑0.2381intergenic (‑107/+118)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (14/11):  ref base (174/182):  total (188/193)
Fisher's exact test strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.97e-01

Only 100 of 390 total aligned reads displayed.

CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283
                                                                                                   |                                                                                                        
ccAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTc                                                                                                      >  2:1079418/1‑101 (MQ=255)
 cAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTca                                                                                                     <  2:718586/101‑2 (MQ=255)
  aaaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTcac                                                                                                    <  2:4873036/101‑3 (MQ=255)
   aaGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCAcc                                                                                                  >  1:1776093/1‑101 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGTAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCAccc                                                                                                 >  1:5792864/1‑101 (MQ=255)
    aGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCAccc                                                                                                 >  2:162995/1‑101 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCAcccc                                                                                                <  1:1324883/101‑2 (MQ=37)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCAcccc                                                                                                >  1:2256036/1‑100 (MQ=255)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCAcccc                                                                                                <  2:5294909/101‑2 (MQ=37)
     gTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.GGCCCCTCCTGGCCTTTCG.G.CCAATTCAccgc                                                                                                >  1:4813219/1‑99 (MQ=255)
       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCACCCcgg                                                                                              >  2:5549146/1‑101 (MQ=37)
       gCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGAAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCACCCcgg                                                                                              >  2:188238/1‑101 (MQ=25)
         cTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGa                                                                                             <  2:5670029/101‑1 (MQ=255)
          tGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGat                                                                                            >  2:6000929/1‑100 (MQ=37)
             aTTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGatgtt                                                                                         >  2:1278140/1‑97 (MQ=25)
                gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTgg                                                                                      >  2:6899964/1‑101 (MQ=25)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                    >  1:219341/1‑101 (MQ=255)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                    >  2:4148034/1‑101 (MQ=255)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                    <  2:3149199/101‑1 (MQ=255)
                  aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGc                                                                                    >  2:2902777/1‑101 (MQ=255)
                   cGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGca                                                                                   >  2:5123367/1‑100 (MQ=25)
                    gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCa                                                                                  >  1:258120/1‑101 (MQ=255)
                     tCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaa                                                                                 >  2:2842080/1‑101 (MQ=255)
                      cTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaag                                                                                >  1:1830359/1‑100 (MQ=21)
                       tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaagg                                                                               >  1:4892078/1‑99 (MQ=255)
                        gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggt                                                                              >  1:1014122/1‑98 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                             <  1:6161314/101‑5 (MQ=255)
                         tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtg                                                                             >  2:786928/1‑97 (MQ=19)
                          ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCCCCCCGGGATGTTTGGGCCaaggtga                                                                            <  2:4059024/101‑6 (MQ=18)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           <  2:1156684/101‑7 (MQ=255)
                           tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                           <  1:2905222/101‑7 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCACTTCACCCCGGGATGCTTGGGCCaaggtgaaa                                                                          >  1:780498/1‑94 (MQ=16)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          <  2:6576464/101‑8 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          <  1:156949/101‑8 (MQ=255)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          >  1:2138270/1‑94 (MQ=16)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          >  2:1472336/1‑94 (MQ=16)
                            aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCaaggtgaaa                                                                          >  2:1547628/1‑94 (MQ=16)
                             aTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAac                                                                       <  1:464956/101‑1 (MQ=16)
                              tttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAaca                                                                      <  1:2838599/101‑2 (MQ=255)
                               ttCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacac                                                                     >  2:4200366/1‑99 (MQ=12)
                                tCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacc                                                                    <  2:4503532/101‑4 (MQ=12)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                  >  2:3670423/1‑96 (MQ=12)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                  >  1:1055208/1‑96 (MQ=12)
                                  gAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacacctt                                                                  >  2:2960247/1‑96 (MQ=12)
                                   aaTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.G.CCAATTCACCCCGGGATGTTGGGCCCAA.GGTGAAacacatgc                                                                 >  2:2652325/1‑95 (MQ=255)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                >  1:3617640/1‑94 (MQ=255)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                <  2:6528408/101‑8 (MQ=255)
                                    aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                <  2:6176461/101‑8 (MQ=255)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCCCCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                             <  1:3994475/101‑11 (MQ=255)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                             <  2:5200087/101‑11 (MQ=255)
                                       cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                             <  2:2135942/101‑11 (MQ=255)
                                          gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                        >  1:6785956/1‑101 (MQ=255)
                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                      <  2:4964901/101‑1 (MQ=9)
                                            aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                      >  1:655483/1‑101 (MQ=255)
                                              gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTTGG.TTTTTCTTgg                                                    >  1:6940217/1‑101 (MQ=9)
                                                aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                  >  1:1229799/1‑101 (MQ=255)
                                                aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                  >  1:5931783/1‑101 (MQ=255)
                                                aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                  >  2:2492551/1‑101 (MQ=255)
                                                 ttttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcgc                                                 >  1:3788890/1‑101 (MQ=9)
                                                   ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGAC.CAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                               >  2:3436729/1‑101 (MQ=255)
                                                    tGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaaa                                              >  1:4085977/1‑101 (MQ=9)
                                                     gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                             >  1:2726242/1‑101 (MQ=255)
                                                      cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                            <  1:5265399/101‑1 (MQ=255)
                                                       aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                           >  2:2399055/1‑101 (MQ=9)
                                                        aggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACg                                          >  2:4712870/1‑101 (MQ=255)
                                                         ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                         <  1:1113277/101‑1 (MQ=255)
                                                          gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                        >  1:3365817/1‑101 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                       <  1:5549436/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                       <  2:6785602/101‑1 (MQ=9)
                                                           aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                       >  2:1395345/1‑101 (MQ=12)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                     <  1:4713110/101‑1 (MQ=255)
                                                             gggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctct                                     <  2:1820704/101‑1 (MQ=255)
                                                                  agaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                  >  2:4774435/11‑101 (MQ=9)
                                                                   gaggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                 >  2:3219292/10‑101 (MQ=12)
                                                                    aggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTTAAAC..CCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                <  2:172818/93‑1 (MQ=12)
                                                                    aggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                >  1:5381011/9‑101 (MQ=12)
                                                                     ggggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                               <  1:5731232/94‑1 (MQ=255)
                                                                      gggcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                              >  2:5790039/7‑101 (MQ=16)
                                                                        gcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                            <  1:503372/97‑1 (MQ=255)
                                                                        gcgctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                            <  1:2406411/97‑1 (MQ=255)
                                                                          gctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                          >  2:377595/3‑101 (MQ=255)
                                                                          gctGCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                          <  1:4941554/99‑1 (MQ=255)
                                                                            ttccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                        <  2:1014180/99‑1 (MQ=16)
                                                                             gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                       >  2:3564591/1‑101 (MQ=16)
                                                                             gCCCCTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                       <  1:1529695/101‑1 (MQ=255)
                                                                              ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      <  1:4200561/101‑1 (MQ=255)
                                                                              ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      >  1:3237817/1‑101 (MQ=16)
                                                                              ccccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                      <  2:2284858/101‑1 (MQ=19)
                                                                                ccTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                    >  1:6362149/1‑101 (MQ=255)
                                                                                 cTCCTGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                   >  1:3156326/1‑101 (MQ=16)
                                                                                   cctGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                 <  2:2720195/99‑1 (MQ=19)
                                                                                    ctGGCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCgcgc                >  1:7020950/2‑101 (MQ=19)
                                                                                      ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg              >  2:4358918/1‑101 (MQ=255)
                                                                                      ggCCTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg              <  1:6253462/101‑1 (MQ=19)
                                                                                         cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           >  2:958249/1‑101 (MQ=255)
                                                                                         cTTTCG.GCCAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg           >  1:4351097/1‑101 (MQ=255)
                                                                                              cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc       >  1:170561/5‑101 (MQ=255)
                                                                                              cggccAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc       <  1:3019532/97‑1 (MQ=25)
                                                                                                  cAATTCACCCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGTCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc   >  2:3894577/1‑101 (MQ=255)
                                                                                                   aaTTCCCCCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCGTGGCGCAAATCCGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGccc  <  2:606370/101‑1 (MQ=255)
                                                                                                   |                                                                                                        
CCAAAGTGCCTGAATTGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCG.G.CAATTCCACCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCC  >  NC_014034/1696082‑1696283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG