Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,696,193 A→T 100% intergenic (‑121/+104) RCAP_rcc01564 ← / ← mmsA isobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,696,1930A→T100.0% 1073.3 336intergenic (‑121/+104)RCAP_rcc01564/mmsAisobutyryl‑CoA dehydrogenase/methylmalonate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (161/175):  ref base (0/0):  total (161/175)

Only 100 of 347 total aligned reads displayed.

TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGT  >  NC_014034/1696097‑1696293
                                                                                                  |                                                                                                    
 gAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTgg                                                                                                >  2:6899964/1‑101 (MQ=25)
  aaCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTggg                                                                                               >  2:2838497/1‑101 (MQ=255)
   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGc                                                                                              >  2:4148034/1‑101 (MQ=255)
   aCGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGc                                                                                              <  2:3149199/101‑1 (MQ=255)
     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                                            <  2:1847489/101‑1 (MQ=25)
     gTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCa                                                                                            >  2:1113242/1‑101 (MQ=255)
        tGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaagg                                                                                         >  1:904090/1‑99 (MQ=255)
         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                        >  1:4130634/1‑98 (MQ=255)
         gTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggt                                                                                        >  1:1014122/1‑98 (MQ=255)
          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                                       >  2:1427762/1‑97 (MQ=19)
          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                                       <  2:7062267/101‑5 (MQ=255)
          tttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtg                                                                                       >  2:786928/1‑97 (MQ=19)
           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                                      >  1:696536/1‑96 (MQ=255)
           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                                      <  2:2676532/101‑6 (MQ=16)
           ttAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtga                                                                                      <  1:1285366/101‑6 (MQ=16)
            tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                                     <  2:4249807/101‑7 (MQ=255)
            tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaa                                                                                     <  1:2905222/101‑7 (MQ=255)
            tAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGG.CCATTTCACCCCGGGATGTTTGGGCCaaggtgaa                                                                                     <  2:1657252/101‑7 (MQ=12)
             aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                                    >  1:2138270/1‑94 (MQ=16)
             aaTTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCaaggtgaaa                                                                                    >  2:2015217/1‑94 (MQ=16)
                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                          <  1:6599199/101‑8 (MQ=255)
                     aTCCTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgc                                                                          <  2:6176461/101‑8 (MQ=255)
                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        >  2:3772616/1‑92 (MQ=255)
                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        <  1:2201932/101‑10 (MQ=9)
                       ccTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgctt                                                                        <  2:3000454/101‑10 (MQ=9)
                        cTTGCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAacaccttgcttt                                                                       <  1:4686306/101‑11 (MQ=255)
                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                  >  1:6785956/1‑101 (MQ=255)
                           gCACGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTc                                                                  <  1:1886862/101‑1 (MQ=255)
                             aCGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCtt                                                                <  2:4608307/101‑1 (MQ=9)
                              cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                               >  2:5143478/1‑101 (MQ=255)
                              cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                               <  2:5005772/101‑1 (MQ=255)
                              cGAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTg                                                               <  2:2430718/101‑1 (MQ=255)
                               gAATTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTgg                                                              >  1:5537181/1‑101 (MQ=9)
                                aaTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgc                                                             >  2:6124276/1‑101 (MQ=255)
                                 aTTTTGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGgcg                                                            >  1:1229799/1‑101 (MQ=255)
                                   tttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCa                                                          >  1:4950128/1‑101 (MQ=255)
                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                         >  2:221816/1‑101 (MQ=255)
                                    ttGCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCaa                                                         <  1:2712860/101‑1 (MQ=255)
                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                       >  1:1775946/1‑101 (MQ=255)
                                      gCAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAAt                                                       >  2:6533408/1‑101 (MQ=255)
                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                      >  1:6253751/1‑101 (MQ=255)
                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                      <  1:5265399/101‑1 (MQ=255)
                                       cAAGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATa                                                      <  2:6643829/101‑1 (MQ=255)
                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATAc                                                     <  2:515264/101‑1 (MQ=255)
                                        aaGGAGGGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATAc                                                     <  2:1601832/101‑1 (MQ=255)
                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                   <  1:3995862/101‑1 (MQ=255)
                                          ggaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGc                                                   <  1:1113277/101‑1 (MQ=255)
                                           gaggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGcc                                                  >  1:4465210/1‑101 (MQ=9)
                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                 <  2:6785602/101‑1 (MQ=9)
                                            aggGGAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCct                                                 >  2:2591494/1‑101 (MQ=12)
                                             ggggAGAGGGGC.GC.TGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCctc                                                <  1:1556075/101‑1 (MQ=255)
                                                   agaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                            >  2:5825785/11‑101 (MQ=9)
                                                   agaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGc                                            >  2:4774435/11‑101 (MQ=9)
                                                    gaggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGcc                                           >  2:3219292/10‑101 (MQ=12)
                                                     aggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGccc                                          <  2:5603685/93‑1 (MQ=255)
                                                      ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                         <  1:5731232/94‑1 (MQ=255)
                                                      ggggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCg                                         >  1:3298151/8‑101 (MQ=12)
                                                       gggcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGa                                        <  1:3100608/95‑1 (MQ=12)
                                                         gcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                      <  1:503372/97‑1 (MQ=255)
                                                         gcgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAg                                      <  1:2842182/97‑1 (MQ=255)
                                                          cgctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAgg                                     >  2:4445869/4‑101 (MQ=16)
                                                           gctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAggg                                    >  1:3612692/3‑101 (MQ=16)
                                                            ctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                   <  1:4925312/100‑1 (MQ=255)
                                                            ctGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGc                                   >  1:707403/2‑101 (MQ=16)
                                                             ttccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                  <  2:1014180/99‑1 (MQ=16)
                                                             tGCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCa                                  >  1:4839139/1‑101 (MQ=19)
                                                              gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                 <  1:2574605/101‑1 (MQ=255)
                                                              gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                 >  2:3564591/1‑101 (MQ=16)
                                                              gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                 <  1:3789188/101‑1 (MQ=255)
                                                              gCCCCTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCaa                                 <  2:180309/101‑1 (MQ=255)
                                                               ccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                <  1:4200561/101‑1 (MQ=255)
                                                               ccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                <  1:2371461/101‑1 (MQ=255)
                                                               ccccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAg                                >  2:4009293/1‑101 (MQ=16)
                                                                 ccTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                              >  1:6362149/1‑101 (MQ=255)
                                                                 ccTCCTGGCCTTGCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGcc                              <  2:2874219/101‑1 (MQ=16)
                                                                  cTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                             <  2:656112/101‑1 (MQ=19)
                                                                  cTCCTGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcg                             >  2:1195833/1‑101 (MQ=16)
                                                                    cctGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCcgcg                           <  2:334117/99‑1 (MQ=19)
                                                                      tGGCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGcc                         <  1:615886/101‑1 (MQ=19)
                                                                       ggCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                        >  2:4358918/1‑101 (MQ=255)
                                                                       ggCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                        >  2:2921575/1‑101 (MQ=255)
                                                                       ggCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                        >  1:579649/1‑101 (MQ=255)
                                                                       ggCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCg                        <  2:1524126/101‑1 (MQ=19)
                                                                        gCCTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGa                       <  2:4891834/101‑1 (MQ=19)
                                                                         ccTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGaa                      <  1:3670572/101‑1 (MQ=19)
                                                                          cTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                     >  2:958249/1‑101 (MQ=255)
                                                                          cTTTCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAg                     <  2:5735456/101‑1 (MQ=19)
                                                                            ttCGGCCAATTCCC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                   <  1:2810206/101‑1 (MQ=25)
                                                                            ttCGGCCAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgc                   >  2:2357446/1‑101 (MQ=255)
                                                                             tcggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcg                  <  1:105952/96‑1 (MQ=19)
                                                                              cggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                 >  2:5291367/5‑101 (MQ=255)
                                                                              cggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                 >  1:170561/5‑101 (MQ=255)
                                                                              cggccAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGgcgc                 <  1:3019532/97‑1 (MQ=25)
                                                                                  cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CACCTT.GCTTTTTCTTGGCGCAAATACGCCTCTGTCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc             >  2:3894577/1‑101 (MQ=255)
                                                                                  cAATTCAC.CCCGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcc             >  2:1289015/1‑101 (MQ=255)
                                                                                     attcaccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGcccc           >  2:5989582/7‑101 (MQ=255)
                                                                                        ccccccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCt        <  1:6032882/98‑1 (MQ=25)
                                                                                         cccccGGGATGTTTGGGCCAA..GGTGAAAC..GCCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTg       <  1:4324919/99‑1 (MQ=25)
                                                                                           cccGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGAt     <  1:3219408/101‑1 (MQ=37)
                                                                                             cGGGATGTTTGGGCCAA..GGTGAAA.CA.CCTTGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCg   <  1:1541287/101‑1 (MQ=37)
                                                                                                  |                                                                                                    
TGAACGTCTGTTTAATTTCGAATCCTTGCACGAATTTTGCAAGGAGGGGAGAAGGGCAGCTTGCCCCTCATGGCCTTTCGG.CAATTCCA.CCCGGGAAGGTTGGGCCAAAGGGTGAAACCACCCTGGCTTTTTCTTGGCGCAAATACGCCTCTGGCCCGAAGGGCAAGCCGCGCCGAAGGCGCGGCCCCCCTGATCGT  >  NC_014034/1696097‑1696293

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG