Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,874 A→G 1.3% intergenic (‑127/+66) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8740A→G 1.3% 2.2 ‑4.3 ‑0.7371intergenic (‑127/+66)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (153/213):  total (158/213)
Fisher's exact test strand distribution p-value = 1.35e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.99e-01

Only 100 of 387 total aligned reads displayed.

AGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTT  >  NC_014034/1734775‑1734974
                                                                                                     |                                                                                                      
agcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTa                                                                                                        >  2:6720633/1‑101 (MQ=255)
agcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTa                                                                                                        <  2:2016187/101‑1 (MQ=255)
 gcggcaggggCGGGCAAGCCAAAGGTTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                                                       <  1:990903/95‑1 (MQ=21)
  cagcGGGGGCGGGCAAGCCAAAGGCGGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                                      <  2:1263634/101‑1 (MQ=21)
  cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                                                      <  1:6889925/101‑1 (MQ=255)
     cgggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCa                                                                                                   <  1:318776/99‑1 (MQ=255)
     ccGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCAGGGATATTTATGGCa                                                                                                   >  2:5902903/1‑101 (MQ=255)
      cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATCTTTATGGCaa                                                                                                  >  2:6368964/1‑101 (MQ=255)
      cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCaa                                                                                                  >  1:5149085/1‑101 (MQ=255)
      cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCaa                                                                                                  >  1:3999425/1‑101 (MQ=255)
       ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                                                                 <  1:4792896/101‑1 (MQ=255)
       ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                                                                 >  1:6045372/1‑101 (MQ=255)
       ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                                                                 <  2:2726459/101‑1 (MQ=255)
       ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCCTCCTGCGGACTCCCCCCCGGGGATTTTTGGCAAg                                                                                                 >  1:903815/1‑101 (MQ=21)
       ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGAGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                                                                 <  2:4865947/101‑1 (MQ=255)
          gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATg                                                                                              <  1:7042249/101‑1 (MQ=255)
           cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGa                                                                                             <  1:6401027/101‑1 (MQ=255)
            gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaa                                                                                            <  1:4265834/101‑1 (MQ=255)
            ggccAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaa                                                                                            <  2:6091008/98‑1 (MQ=255)
              gcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAc                                                                                          >  2:855267/1‑101 (MQ=255)
               cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAcc                                                                                         >  1:6927782/1‑100 (MQ=255)
               cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACt                                                                                         >  2:4190974/1‑101 (MQ=255)
                aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                        <  2:2499223/101‑1 (MQ=255)
                aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                        <  1:2056960/101‑1 (MQ=255)
                 aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGc                                                                                       <  2:2633429/101‑1 (MQ=255)
                    cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                                                                   >  2:3515594/1‑101 (MQ=255)
                     aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                  <  2:5578638/101‑1 (MQ=255)
                     aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                  >  1:1098639/1‑101 (MQ=255)
                      aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                 >  1:1217533/1‑101 (MQ=255)
                       aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                <  2:905469/101‑1 (MQ=255)
                        ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                               <  1:1749815/101‑1 (MQ=255)
                        ggCGGACGCTCCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                               <  2:5286570/101‑1 (MQ=18)
                         gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAACCTGC.GGCCTTCa                                                                              <  1:5052890/101‑1 (MQ=255)
                          cTGCCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                             <  2:2218964/101‑1 (MQ=255)
                          cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                             >  2:2389976/1‑101 (MQ=255)
                            gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                           <  2:3647018/101‑1 (MQ=255)
                             gcGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                          <  1:1022391/100‑1 (MQ=255)
                              cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                         <  1:1964226/101‑1 (MQ=255)
                              cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                         <  1:6539861/101‑1 (MQ=255)
                               gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                        <  2:3352413/101‑1 (MQ=255)
                               gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGAGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                        <  1:3547267/101‑1 (MQ=255)
                                 tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                      >  2:5758195/1‑101 (MQ=255)
                                 tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAATGC.GGCCTTCCTCTTTCCa                                                                      >  2:6284425/1‑101 (MQ=17)
                                   cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                    <  2:1045509/101‑1 (MQ=255)
                                   cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                    >  1:302134/1‑101 (MQ=255)
                                   cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGACTTCATATTGGCATa                                                                    >  2:694786/1‑101 (MQ=17)
                                    gTCAGCCGANGGNNNGNGCAGGNNGGNGCNNCCCC.CCNTCCTGCGGACTCCCNCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                   <  1:1809698/101‑1 (MQ=18)
                                     tcggcagACGGTCCGGGCAGGGGTGCGCTGCCCC.CCGTCCTGCGCACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                  <  2:3035703/95‑1 (MQ=14)
                                     tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                  <  1:5263220/101‑1 (MQ=255)
                                     tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                  <  2:1761788/101‑1 (MQ=255)
                                     tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                  <  2:5126103/101‑1 (MQ=255)
                                      cAGCCTACGGGCCTGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                 <  2:2282432/101‑1 (MQ=17)
                                      cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                 >  1:218161/1‑101 (MQ=255)
                                      cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                 <  2:1131963/101‑1 (MQ=255)
                                       aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                <  1:5873827/101‑1 (MQ=255)
                                        gCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                               <  1:1489414/101‑1 (MQ=255)
                                         ccGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCAGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                              <  1:5903206/101‑1 (MQ=255)
                                          cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                             <  2:1563566/101‑1 (MQ=255)
                                          cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                             <  2:4751045/101‑1 (MQ=255)
                                           gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                            <  1:5503760/101‑1 (MQ=255)
                                           gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                            <  1:6284748/101‑1 (MQ=255)
                                            gcgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                          <  2:5106193/100‑1 (MQ=255)
                                                tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                     >  2:6542554/9‑101 (MQ=2)
                                                gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                      >  1:5807041/1‑101 (MQ=255)
                                                 gacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                    >  2:4094258/8‑100 (MQ=2)
                                                 ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                     <  1:3793242/101‑1 (MQ=255)
                                                  ggggcTGGGGGGCGCTC.CCCGCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATGTCCC.GGGGTGc                                                    <  2:361738/100‑1 (MQ=17)
                                                  cgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                    <  2:3167905/101‑1 (MQ=255)
                                                  acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                   >  2:4536527/7‑99 (MQ=2)
                                                   cggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                  >  1:4349599/6‑98 (MQ=2)
                                                      cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                <  1:2486505/101‑1 (MQ=255)
                                                      cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCgggg                                                >  1:3340748/1‑101 (MQ=255)
                                                       aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                               >  1:4437213/1‑101 (MQ=255)
                                                           gggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCagag                                           <  1:4162207/101‑1 (MQ=255)
                                                            ggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                          <  1:5336712/101‑1 (MQ=255)
                                                               gctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                       >  1:7001833/6‑101 (MQ=255)
                                                                ctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                      >  2:5462949/5‑101 (MQ=255)
                                                                   cccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                   <  1:4949540/100‑1 (MQ=255)
                                                                        ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                               <  2:6824629/101‑1 (MQ=255)
                                                                        ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                               <  1:4312509/101‑1 (MQ=255)
                                                                          gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                             >  2:714239/1‑101 (MQ=255)
                                                                               gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                        <  2:5818121/101‑1 (MQ=255)
                                                                                cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                       >  1:4473757/1‑101 (MQ=255)
                                                                                  gACTCCCCCCGGGATATTTATGGCAAGATGAATCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                     <  2:329526/101‑1 (MQ=255)
                                                                                  gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                     <  2:661616/101‑1 (MQ=255)
                                                                                    cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                   >  2:1952902/1‑101 (MQ=255)
                                                                                     tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                  >  1:6699167/1‑101 (MQ=255)
                                                                                        ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc               <  1:753740/101‑1 (MQ=255)
                                                                                         cccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcg              <  1:2652125/101‑1 (MQ=255)
                                                                                          ccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc             <  1:325022/101‑1 (MQ=255)
                                                                                           cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg            >  2:4960481/1‑101 (MQ=255)
                                                                                            gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg           >  2:380304/1‑101 (MQ=255)
                                                                                              gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc         <  2:648705/101‑1 (MQ=255)
                                                                                               atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc        >  2:2947332/1‑101 (MQ=255)
                                                                                               atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc        >  1:1316272/1‑101 (MQ=255)
                                                                                                 atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg      <  1:4582653/101‑1 (MQ=255)
                                                                                                  tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt     >  1:1561976/1‑101 (MQ=255)
                                                                                                   ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt    >  1:4671920/1‑101 (MQ=255)
                                                                                                    tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt   >  1:2828973/1‑101 (MQ=255)
                                                                                                     aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt  <  1:511001/101‑1 (MQ=255)
                                                                                                     |                                                                                                      
AGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTT  >  NC_014034/1734775‑1734974

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG