Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,887 A→G 1.6% intergenic (‑140/+53) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8870A→G 1.6% 2.5 ‑2.2 ‑0.1354intergenic (‑140/+53)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (164/182):  total (169/185)
Fisher's exact test strand distribution p-value = 1.09e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.40e-01

Only 100 of 379 total aligned reads displayed.

GGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCAA  >  NC_014034/1734788‑1734987
                                                                                                     |                                                                                                       
ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGaaa                                                                                                         >  1:1543337/1‑101 (MQ=255)
 gcAAGCCAAGGGCTGACGCTGCGTCAGCCGGCGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAc                                                                                                        <  1:6640123/101‑1 (MQ=18)
  cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAcc                                                                                                       >  1:6927782/1‑100 (MQ=255)
  cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACt                                                                                                       >  2:4190974/1‑101 (MQ=255)
   aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                      >  2:882672/1‑101 (MQ=255)
    aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGc                                                                                                     >  2:6370592/1‑101 (MQ=255)
     gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                   >  2:5172308/1‑101 (MQ=255)
     gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                   >  2:7068793/1‑101 (MQ=255)
        aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCGCAGTCCTGAGGACTCCCCCCGGGATATTTGTGGGAAGATGAAACGGG.GGcc                                                                                                >  1:3340350/1‑101 (MQ=18)
         aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                               <  1:5845797/101‑1 (MQ=255)
          aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                              <  1:454192/101‑1 (MQ=255)
           ggCGGACGCTCCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                             <  2:5286570/101‑1 (MQ=18)
             cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                           >  2:2389976/1‑101 (MQ=255)
             cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                           <  1:3202874/101‑1 (MQ=255)
              tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                                                                          >  1:1734127/1‑101 (MQ=255)
               gACGCTGCGTCCCCCGACGGGCCGGGCAGGGGGGCCCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                         <  1:2280242/101‑1 (MQ=18)
               gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                         <  2:3647018/101‑1 (MQ=255)
               gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTACTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                         <  1:2573920/101‑1 (MQ=255)
                 cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                       <  2:2241795/101‑1 (MQ=255)
                  gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                      <  2:3352413/101‑1 (MQ=255)
                  gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGAGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                      <  1:3547267/101‑1 (MQ=255)
                   cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                                                                                     <  2:4751341/101‑1 (MQ=255)
                    tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAATGC.GGCCTTCCTCTTTCCa                                                                                    >  2:6284425/1‑101 (MQ=17)
                     gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                   <  1:5127999/101‑1 (MQ=255)
                      ggTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                  <  2:5981908/100‑1 (MQ=255)
                        tcggcagACGGTCCGGGCAGGGGTGCGCTGCCCC.CCGTCCTGCGCACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                <  2:3035703/95‑1 (MQ=14)
                        tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                <  1:7017028/101‑1 (MQ=255)
                        tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                <  2:1761788/101‑1 (MQ=255)
                        tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                <  1:5263220/101‑1 (MQ=255)
                         cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                               <  2:1131963/101‑1 (MQ=255)
                          aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                              <  1:5873827/101‑1 (MQ=255)
                          aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                              <  2:6494078/101‑1 (MQ=255)
                             cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                           <  1:4711680/101‑1 (MQ=255)
                             cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                           <  2:1563566/101‑1 (MQ=255)
                              gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                          <  2:1065517/101‑1 (MQ=255)
                                 gggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                      <  1:6117497/101‑1 (MQ=255)
                                   tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                   >  2:6542554/9‑101 (MQ=2)
                                    gacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                  >  2:4094258/8‑100 (MQ=2)
                                     acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                 >  2:4536527/7‑99 (MQ=2)
                                      cggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                >  1:4349599/6‑98 (MQ=2)
                                      cggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                >  1:6430033/6‑98 (MQ=2)
                                         cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                              >  1:1314180/1‑101 (MQ=255)
                                          aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGTCATAAATATCAC.GGGGTGCGGGGc                                                             >  2:2023470/1‑101 (MQ=17)
                                          aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                             >  1:179200/1‑101 (MQ=255)
                                          aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                             >  1:4437213/1‑101 (MQ=255)
                                          aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                             <  2:5800419/101‑1 (MQ=255)
                                          aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                             >  2:3595971/1‑101 (MQ=255)
                                           ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                            <  1:946428/101‑1 (MQ=255)
                                               ggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                        <  1:5336712/101‑1 (MQ=255)
                                                gcgcTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                       <  2:4829791/101‑1 (MQ=255)
                                                 cgcTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCGTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcag                                                      >  1:743191/1‑99 (MQ=255)
                                                  gctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                     <  1:2113983/96‑1 (MQ=255)
                                                     gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                  <  2:5107066/99‑1 (MQ=255)
                                                       ccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGCGCAGAGCCCCGCTCa                                                <  1:5623954/101‑1 (MQ=255)
                                                        cc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                               >  2:5495475/1‑101 (MQ=255)
                                                           ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                             <  1:4312509/101‑1 (MQ=255)
                                                            cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                            <  1:4158889/101‑1 (MQ=255)
                                                             gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                           <  2:3494386/101‑1 (MQ=255)
                                                             gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                           >  1:4651167/1‑101 (MQ=255)
                                                                   cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                     >  1:1036477/1‑101 (MQ=255)
                                                                   cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                     >  1:4836940/1‑101 (MQ=255)
                                                                   cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                     >  1:4473757/1‑101 (MQ=255)
                                                                    ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                    >  2:5875221/1‑101 (MQ=255)
                                                                     gACTCCCCCCGGGATATTTATGGCAAGATGAATCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                   <  2:329526/101‑1 (MQ=255)
                                                                       cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                 >  1:3029681/1‑101 (MQ=255)
                                                                           ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                             <  2:2653166/101‑1 (MQ=255)
                                                                              cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc.g                         >  1:395959/1‑101 (MQ=255)
                                                                               gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.gg                        >  2:2807930/1‑101 (MQ=255)
                                                                               gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.gg                        >  2:380304/1‑101 (MQ=255)
                                                                                ggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGc                       >  2:2607706/1‑101 (MQ=255)
                                                                                 gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGcc                      >  2:6794514/1‑101 (MQ=255)
                                                                                  atatTTATGGCAGGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGGTCCGC.GGccc                     >  1:5076591/1‑101 (MQ=255)
                                                                                  atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGccc                     >  2:5619257/1‑101 (MQ=255)
                                                                                  atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGccc                     >  1:2019414/1‑101 (MQ=255)
                                                                                  atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGccc                     <  2:2326730/101‑1 (MQ=255)
                                                                                     tttATGGCAAGATGAAACTGC.GGCTTTAATTTTGCCATAAAAATCCG.GGGGGGCGGGGAAGAGCCCGGC.CAACCGGAAGTGACAAGATCCGCGGGCCCGGt                  >  2:6681382/1‑101 (MQ=255)
                                                                                     tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGt                  >  1:2769623/1‑101 (MQ=255)
                                                                                     tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGt                  >  2:3187763/1‑101 (MQ=255)
                                                                                      ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGtt                 >  2:5028464/1‑101 (MQ=255)
                                                                                       tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGttt                >  1:1195099/1‑101 (MQ=255)
                                                                                           gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGc            >  1:3490581/1‑101 (MQ=255)
                                                                                           gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGc            >  1:3664817/1‑101 (MQ=255)
                                                                                            cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCt           >  1:1631014/1‑101 (MQ=255)
                                                                                            cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCt           >  2:7097756/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg          >  1:5934203/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg          >  2:6879180/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg          >  1:1896496/1‑101 (MQ=255)
                                                                                             aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGCGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg          >  2:5838506/1‑101 (MQ=255)
                                                                                              aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtga         >  2:1970109/1‑101 (MQ=255)
                                                                                              aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtga         <  1:4191169/101‑1 (MQ=255)
                                                                                               gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtgat        <  1:6519421/101‑1 (MQ=255)
                                                                                                 tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGCTGACAAGAACCGC.AGCCCGGTTTTCGCttctga      >  2:913641/1‑96 (MQ=25)
                                                                                                 tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtgatga      <  1:7048734/101‑1 (MQ=255)
                                                                                                   aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGc    >  2:1290306/1‑101 (MQ=255)
                                                                                                   aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGc    <  2:771627/101‑1 (MQ=255)
                                                                                                   aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGc    >  1:2880758/1‑101 (MQ=255)
                                                                                                    aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCa   <  1:882696/101‑1 (MQ=255)
                                                                                                     aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa  <  1:6813387/101‑1 (MQ=255)
                                                                                                     aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa  <  1:1429450/101‑1 (MQ=255)
                                                                                                     aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa  <  1:1006223/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
GGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCAA  >  NC_014034/1734788‑1734987

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG