Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,888 C→G 1.4% intergenic (‑141/+52) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8880C→G 1.4% 2.2 ‑3.8 ‑0.2365intergenic (‑141/+52)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (165/194):  total (171/194)
Fisher's exact test strand distribution p-value = 2.15e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.13e-01

Only 100 of 376 total aligned reads displayed.

GCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCAAG  >  NC_014034/1734789‑1734988
                                                                                                     |                                                                                                       
 cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAcc                                                                                                        >  1:6927782/1‑100 (MQ=255)
  aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       >  2:882672/1‑101 (MQ=255)
  aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       <  1:2056960/101‑1 (MQ=255)
  aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       <  2:2499223/101‑1 (MQ=255)
   aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGc                                                                                                      <  1:4314771/101‑1 (MQ=255)
   aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGc                                                                                                      >  2:6370592/1‑101 (MQ=255)
    gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                    >  2:5172308/1‑101 (MQ=255)
    gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                    >  2:7068793/1‑101 (MQ=255)
     ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                                                   >  1:884072/1‑101 (MQ=255)
       aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCGCAGTCCTGAGGACTCCCCCCGGGATATTTGTGGGAAGATGAAACGGG.GGcc                                                                                                 >  1:3340350/1‑101 (MQ=18)
          ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                              <  1:1749815/101‑1 (MQ=255)
            cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                            <  1:3202874/101‑1 (MQ=255)
             tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                                                                           <  2:2742257/101‑1 (MQ=255)
              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                          <  2:3647018/101‑1 (MQ=255)
              gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTACTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                          <  1:2573920/101‑1 (MQ=255)
                cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                        <  1:6539861/101‑1 (MQ=255)
                cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                        >  2:2113903/1‑101 (MQ=255)
                cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                        <  2:2241795/101‑1 (MQ=255)
                  cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGcc                                                                                      <  2:4751341/101‑1 (MQ=255)
                   tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                     >  2:5758195/1‑101 (MQ=255)
                   tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAAATGC.GGCCTTCCTCTTTCCa                                                                                     >  2:6284425/1‑101 (MQ=17)
                    gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  1:5127999/101‑1 (MQ=255)
                      gTCAGCCGANGGNNNGNGCAGGNNGGNGCNNCCCC.CCNTCCTGCGGACTCCCNCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                  <  1:1809698/101‑1 (MQ=18)
                       tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 <  1:5263220/101‑1 (MQ=255)
                       tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 <  2:1761788/101‑1 (MQ=255)
                       tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 <  1:7017028/101‑1 (MQ=255)
                        cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                >  1:218161/1‑101 (MQ=255)
                         aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATCAata                                                                               >  2:3323076/1‑101 (MQ=255)
                         aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                               <  2:6494078/101‑1 (MQ=255)
                         aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAata                                                                               <  1:5873827/101‑1 (MQ=255)
                          gCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                              >  2:5556949/1‑101 (MQ=255)
                           ccGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                             <  1:7092982/101‑1 (MQ=255)
                            cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                            <  1:4711680/101‑1 (MQ=255)
                            cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                            <  2:1563566/101‑1 (MQ=255)
                             gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                           <  1:6284748/101‑1 (MQ=255)
                             gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                           <  2:1065517/101‑1 (MQ=255)
                               cttccgatctaGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                        <  2:4110157/91‑1 (MQ=14)
                               cgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                        >  2:6148026/1‑101 (MQ=255)
                               cgggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                                                                        >  1:6824983/1‑101 (MQ=255)
                                gggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                       <  2:1518542/101‑1 (MQ=255)
                                  gccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                                                                     >  1:5807041/1‑101 (MQ=255)
                                   gcgggcAGCGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                    <  2:309756/100‑1 (MQ=255)
                                    ggggcTGGGGGGCGCTC.CCCGCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATGTCCC.GGGGTGc                                                                   <  2:361738/100‑1 (MQ=17)
                                    cgggcAGGGGGGCCCTGCCCC.CCGTCCTGCGGACGCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                   <  2:5395883/101‑1 (MQ=14)
                                     cggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                 >  1:4349599/6‑98 (MQ=2)
                                     cggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                 >  1:6430033/6‑98 (MQ=2)
                                        cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                               >  1:1314180/1‑101 (MQ=255)
                                         aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                              >  1:4437213/1‑101 (MQ=255)
                                         aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                              <  2:5800419/101‑1 (MQ=255)
                                          ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                             <  1:946428/101‑1 (MQ=255)
                                              ggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                         <  1:5336712/101‑1 (MQ=255)
                                               gcgcTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                        >  1:2893818/1‑101 (MQ=255)
                                                    gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   >  1:3123532/3‑101 (MQ=255)
                                                     cccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                  >  1:3560767/2‑101 (MQ=255)
                                                      ccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGCGCAGAGCCCCGCTCa                                                 <  1:5623954/101‑1 (MQ=255)
                                                          ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                              <  2:3262501/101‑1 (MQ=255)
                                                           cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                             <  1:4158889/101‑1 (MQ=255)
                                                            gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                            >  1:4651167/1‑101 (MQ=255)
                                                            gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                            >  2:35392/1‑101 (MQ=255)
                                                                 gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                       <  1:4258372/101‑1 (MQ=255)
                                                                  cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                      >  1:4836940/1‑101 (MQ=255)
                                                                  cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                      >  1:4473757/1‑101 (MQ=255)
                                                                  cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                      >  1:1036477/1‑101 (MQ=255)
                                                                   ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                     >  2:4907478/1‑101 (MQ=255)
                                                                   ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                     <  2:6547320/101‑1 (MQ=255)
                                                                       tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                 >  1:6699167/1‑101 (MQ=255)
                                                                        ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                <  1:1651951/101‑1 (MQ=255)
                                                                          ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                              <  1:753740/101‑1 (MQ=255)
                                                                             cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgc.g                          >  1:395959/1‑101 (MQ=255)
                                                                                gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGcc                       >  2:6794514/1‑101 (MQ=255)
                                                                                 atatTTATGGCAGGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGGTCCGC.GGccc                      >  1:5076591/1‑101 (MQ=255)
                                                                                 atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGccc                      >  2:2640025/1‑101 (MQ=255)
                                                                                 atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGccc                      <  2:2326730/101‑1 (MQ=255)
                                                                                  tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCg                     <  2:1168137/101‑1 (MQ=255)
                                                                                   atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCgg                    >  2:425546/1‑101 (MQ=255)
                                                                                    tttATGGCAAGATGAAACTGC.GGCTTTAATTTTGCCATAAAAATCCG.GGGGGGCGGGGAAGAGCCCGGC.CAACCGGAAGTGACAAGATCCGCGGGCCCGGt                   >  2:6681382/1‑101 (MQ=255)
                                                                                    tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGt                   >  1:1561976/1‑101 (MQ=255)
                                                                                    tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGt                   >  1:2769623/1‑101 (MQ=255)
                                                                                    tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGt                   >  2:2958082/1‑101 (MQ=255)
                                                                                      tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGttt                 >  2:5109002/1‑101 (MQ=255)
                                                                                      tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGttt                 >  1:1195099/1‑101 (MQ=255)
                                                                                          gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGc             >  1:3664817/1‑101 (MQ=255)
                                                                                          gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGc             >  1:3490581/1‑101 (MQ=255)
                                                                                           cAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCt            >  2:7097756/1‑101 (MQ=255)
                                                                                            aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg           >  1:5934203/1‑101 (MQ=255)
                                                                                            aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg           >  2:6879180/1‑101 (MQ=255)
                                                                                            aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg           >  2:5898339/1‑101 (MQ=255)
                                                                                            aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGCGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg           >  2:5838506/1‑101 (MQ=255)
                                                                                            aaGATGAAACTGC.GGCCTTCATCTAGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtg           >  2:3402552/1‑101 (MQ=255)
                                                                                             aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtga          >  2:1970109/1‑101 (MQ=255)
                                                                                               aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCtgatg        >  1:2299060/1‑101 (MQ=255)
                                                                                                tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGCTGACAAGAACCGC.AGCCCGGTTTTCGCttctga       >  2:913641/1‑96 (MQ=25)
                                                                                                 gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAg      <  2:4721566/101‑1 (MQ=255)
                                                                                                 gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAg      >  2:3856032/1‑101 (MQ=255)
                                                                                                  aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGc     <  2:771627/101‑1 (MQ=255)
                                                                                                   aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCa    <  1:455220/101‑1 (MQ=255)
                                                                                                    aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa   <  2:5040016/101‑1 (MQ=255)
                                                                                                    aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa   >  1:1927639/1‑101 (MQ=255)
                                                                                                    aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa   <  1:1429450/101‑1 (MQ=255)
                                                                                                    aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCaa   <  1:1006223/101‑1 (MQ=255)
                                                                                                     |                                                                                                       
GCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGC.GGCCCGGTTTTCGCTGATGAGCAAG  >  NC_014034/1734789‑1734988

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG