Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,889 T→G 1.4% intergenic (‑142/+51) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8890T→G 1.4% 3.1 ‑2.3 ‑0.1362intergenic (‑142/+51)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (165/191):  total (170/192)
Fisher's exact test strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.02e-01

Only 100 of 376 total aligned reads displayed.

CAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGC  >  NC_014034/1734790‑1734989
                                                                                                     |                                                                                                      
cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACt                                                                                                        <  1:4727455/101‑1 (MQ=255)
cAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACt                                                                                                        >  2:4190974/1‑101 (MQ=255)
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       >  1:5883362/1‑101 (MQ=255)
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       >  2:882672/1‑101 (MQ=255)
 aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTg                                                                                                       <  1:2056960/101‑1 (MQ=255)
  aGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGc                                                                                                      <  2:2633429/101‑1 (MQ=255)
    ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                                                   >  1:884072/1‑101 (MQ=255)
     cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                                                                                  >  2:3515594/1‑101 (MQ=255)
     cAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGc                                                                                                  <  2:5828685/101‑1 (MQ=255)
      aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCGCAGTCCTGAGGACTCCCCCCGGGATATTTGTGGGAAGATGAAACGGG.GGcc                                                                                                 >  1:3340350/1‑101 (MQ=18)
        aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                               <  2:5783024/101‑1 (MQ=255)
         ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                              <  1:4538487/101‑1 (MQ=255)
         ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                              <  1:1749815/101‑1 (MQ=255)
         ggCGGACGCTCCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTc                                                                                              <  2:5286570/101‑1 (MQ=18)
           cTGCCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                            <  2:2218964/101‑1 (MQ=255)
           cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                            <  1:3202874/101‑1 (MQ=255)
           cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                            >  2:2389976/1‑101 (MQ=255)
             gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTACTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                          <  1:2573920/101‑1 (MQ=255)
              aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                         <  1:6506038/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  1:5672749/101‑1 (MQ=255)
                   gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  1:6023295/101‑1 (MQ=255)
                   gCGGCAGCCGACGGGCCGGGCAGGGGGCCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                    <  2:6863564/101‑1 (MQ=17)
                    ggTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                   <  2:5981908/100‑1 (MQ=255)
                     gTCAGCCGANGGNNNGNGCAGGNNGGNGCNNCCCC.CCNTCCTGCGGACTCCCNCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                  <  1:1809698/101‑1 (MQ=18)
                      tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 <  1:7017028/101‑1 (MQ=255)
                      tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                 <  2:5126103/101‑1 (MQ=255)
                       cAGCCTACGGGCCTGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                <  2:2282432/101‑1 (MQ=17)
                       cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                >  1:218161/1‑101 (MQ=255)
                          ccGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                             <  1:7092982/101‑1 (MQ=255)
                           cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                            <  2:3640543/101‑1 (MQ=255)
                           cGACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                            <  2:4751045/101‑1 (MQ=255)
                            gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                           <  2:1065517/101‑1 (MQ=255)
                            gACGGGCCGGGCAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                           <  1:4328128/101‑1 (MQ=255)
                                ggccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                      >  1:4127672/1‑101 (MQ=255)
                                 tgacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                    >  2:6542554/9‑101 (MQ=2)
                                  gacggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                   >  2:4094258/8‑100 (MQ=2)
                                  ccgggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTGCCCCCGGGATATTTATGGCAAGAGGAAACGGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                    <  1:6369288/101‑1 (MQ=14)
                                   acggacggggggCTCTG.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa                                                                  >  2:4536527/7‑99 (MQ=2)
                                    gggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                  <  1:5172568/101‑1 (MQ=255)
                                    gggcAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                  >  1:4985330/1‑101 (MQ=255)
                                       cAGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg                                                               >  1:1314180/1‑101 (MQ=255)
                                        aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCGCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGAGCGGGGc                                                              <  2:7081224/101‑1 (MQ=17)
                                        aGGGGGGCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGTCATAAATATCAC.GGGGTGCGGGGc                                                              >  2:2023470/1‑101 (MQ=17)
                                         ggggggCGCTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                             <  1:946428/101‑1 (MQ=255)
                                             ctcgcTGCCCC.CCGTCCTGCGGACGCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                         <  2:743167/99‑1 (MQ=255)
                                              gcgcTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                        >  1:2893818/1‑101 (MQ=255)
                                              gcgcTGCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                        <  2:4829791/101‑1 (MQ=255)
                                                gctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTAAGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                      >  1:4311078/6‑101 (MQ=255)
                                                 ctgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                     >  1:4460420/5‑101 (MQ=255)
                                                  tgcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGGCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                    >  1:4365400/4‑101 (MQ=255)
                                                   gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   >  2:6782648/3‑101 (MQ=255)
                                                   gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   <  2:3067296/99‑1 (MQ=255)
                                                   gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   <  2:5107066/99‑1 (MQ=255)
                                                   gcccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                   >  1:3123532/3‑101 (MQ=255)
                                                    cccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                  >  1:3560767/2‑101 (MQ=255)
                                                    cccc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                  <  2:6248872/100‑1 (MQ=255)
                                                      cc.ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCaa                                                >  2:5495475/1‑101 (MQ=255)
                                                                 cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                      >  1:1036477/1‑101 (MQ=255)
                                                                  ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                     >  2:1745107/1‑101 (MQ=255)
                                                                  ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                     >  2:5875221/1‑101 (MQ=255)
                                                                  ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                     >  2:2666804/1‑101 (MQ=255)
                                                                   gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAcc                                    <  2:3192070/101‑2 (MQ=255)
                                                                   gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                    <  2:661616/101‑1 (MQ=255)
                                                                    aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                   >  1:5411843/1‑101 (MQ=255)
                                                                     cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                  >  2:1952902/1‑101 (MQ=255)
                                                                       ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                <  1:1651951/101‑1 (MQ=255)
                                                                         ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                              <  1:753740/101‑1 (MQ=255)
                                                                         ccccGGGATATTTAAGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                              <  1:5285122/101‑1 (MQ=255)
                                                                             gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                          >  2:380304/1‑101 (MQ=255)
                                                                               gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                        >  2:6364948/1‑101 (MQ=255)
                                                                               gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                        <  2:648705/101‑1 (MQ=255)
                                                                                atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                       >  2:5619257/1‑101 (MQ=255)
                                                                                atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                       >  2:2947332/1‑101 (MQ=255)
                                                                                 tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                      <  2:1168137/101‑1 (MQ=255)
                                                                                 tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                      >  1:5847727/1‑101 (MQ=255)
                                                                                  atTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCgg                     <  1:4582653/101‑1 (MQ=255)
                                                                                   tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                    >  1:6918291/1‑101 (MQ=255)
                                                                                   tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                    >  1:2769623/1‑101 (MQ=255)
                                                                                   tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                    >  1:1561976/1‑101 (MQ=255)
                                                                                    ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                   >  2:5028464/1‑101 (MQ=255)
                                                                                    ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                   >  1:4671920/1‑101 (MQ=255)
                                                                                     tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                  <  2:3307593/101‑1 (MQ=255)
                                                                                     tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                  >  1:1195099/1‑101 (MQ=255)
                                                                                      aTGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtttt                 <  1:511001/101‑1 (MQ=255)
                                                                                         gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc              >  1:3664817/1‑101 (MQ=255)
                                                                                         gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc              >  1:3490581/1‑101 (MQ=255)
                                                                                           aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg            >  1:4164879/1‑101 (MQ=255)
                                                                                            aGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtga           >  2:3392144/1‑101 (MQ=255)
                                                                                              aTGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatg         >  1:2299060/1‑101 (MQ=255)
                                                                                               tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGCTGACAAGAACCGCAGCCCGGTTTTCGCttctga        >  2:913641/1‑96 (MQ=25)
                                                                                               tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgatga        >  1:6614373/1‑101 (MQ=255)
                                                                                                gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg       <  2:1556099/101‑1 (MQ=255)
                                                                                                gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg       >  2:700142/1‑101 (MQ=255)
                                                                                                   aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    <  1:6813387/101‑1 (MQ=255)
                                                                                                   aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    >  1:1927639/1‑101 (MQ=255)
                                                                                                   aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    <  1:1429450/101‑1 (MQ=255)
                                                                                                   aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa    <  1:1006223/101‑1 (MQ=255)
                                                                                                    cTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAg   >  2:3596538/1‑101 (MQ=255)
                                                                                                     tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc  <  2:131274/101‑1 (MQ=255)
                                                                                                     tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc  >  1:385786/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGC  >  NC_014034/1734790‑1734989

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG