Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,892 G→T 1.4% intergenic (‑145/+48) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8920G→T 1.4% 4.6 ‑3.8 ‑0.2353intergenic (‑145/+48)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (160/186):  total (166/187)
Fisher's exact test strand distribution p-value = 2.22e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.98e-01

Only 100 of 366 total aligned reads displayed.

GCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCC  >  NC_014034/1734793‑1734992
                                                                                                     |                                                                                                     
gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGAGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.g                                                                                                       >  1:4591573/1‑101 (MQ=255)
 ccAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.gg                                                                                                      >  1:884072/1‑101 (MQ=255)
   aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                                    <  2:1893718/101‑1 (MQ=255)
   aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGcc                                                                                                    <  2:5578638/101‑1 (MQ=255)
    aaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCt                                                                                                   >  1:5126347/1‑101 (MQ=255)
     aGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCtt                                                                                                  <  1:454192/101‑1 (MQ=255)
        cTGCCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                               <  2:2218964/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                               <  1:2040813/101‑1 (MQ=255)
        cTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCAt                                                                                               >  2:2389976/1‑101 (MQ=255)
         tGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATc                                                                                              <  2:2742257/101‑1 (MQ=255)
          gACGCTGCGTCCCCCGACGGGCCGGGCAGGGGGGCCCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                             <  1:2280242/101‑1 (MQ=18)
          gACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTACTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCt                                                                                             <  1:2573920/101‑1 (MQ=255)
           aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCtt                                                                                            <  1:4822843/101‑1 (MQ=255)
            cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTg                                                                                           <  2:790078/101‑1 (MQ=255)
             gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGc                                                                                          <  2:3352413/101‑1 (MQ=255)
                gCGGCAGCCGACGGGCCGGGCAGGGGGCCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                       <  2:6863564/101‑1 (MQ=17)
                 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                      >  1:5240697/1‑101 (MQ=255)
                 cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGACTTCATATTGGCATa                                                                                      >  2:694786/1‑101 (MQ=17)
                  gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                     <  2:5953950/101‑1 (MQ=255)
                  gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                     <  1:3603998/101‑1 (MQ=255)
                  gTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                     <  1:1348772/101‑1 (MQ=255)
                    cAGCCTACGGGCCTGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   <  2:2282432/101‑1 (MQ=17)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   <  2:1131963/101‑1 (MQ=255)
                    cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                   >  1:218161/1‑101 (MQ=255)
                     aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATCAata                                                                                  >  2:3323076/1‑101 (MQ=255)
                      gCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAatat                                                                                 <  2:2618494/101‑1 (MQ=255)
                       ccGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                                <  1:7092982/101‑1 (MQ=255)
                       ccGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                                <  2:5278855/101‑1 (MQ=255)
                       ccGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCAGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                                <  1:5903206/101‑1 (MQ=255)
                        cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                               <  2:4751045/101‑1 (MQ=255)
                         gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                              <  2:1065517/101‑1 (MQ=255)
                         gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                              >  2:3628476/1‑101 (MQ=255)
                          gcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                            <  2:5106193/100‑1 (MQ=255)
                            gggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggg                                                                          <  1:6117497/101‑1 (MQ=255)
                             ggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gggg                                                                         >  1:4127672/1‑101 (MQ=255)
                              tgacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg                                                                       >  2:6542554/9‑101 (MQ=2)
                                 gggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg                                                                     >  1:4985330/1‑101 (MQ=255)
                                 cggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaat                                                                    >  1:4349599/6‑98 (MQ=2)
                                    cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCgggg                                                                  >  1:3340748/1‑101 (MQ=255)
                                     aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCGCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGAGCGGGGc                                                                 <  2:7081224/101‑1 (MQ=17)
                                     aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGTCATAAATATCAC.GGGGTGCGGGGc                                                                 >  2:2023470/1‑101 (MQ=17)
                                     aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                 >  2:3595971/1‑101 (MQ=255)
                                     aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGCTGCGGGGc                                                                 >  1:3307709/1‑101 (MQ=255)
                                      ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                <  1:946428/101‑1 (MQ=255)
                                      ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCa                                                                >  2:4390959/1‑101 (MQ=255)
                                         gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCagag                                                             <  1:4162207/101‑1 (MQ=255)
                                          ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                            >  2:4080136/1‑101 (MQ=255)
                                          ctcgcTGCCCCCCGTCCTGCGGACGCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                            <  2:743167/99‑1 (MQ=255)
                                           gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                           >  1:2893818/1‑101 (MQ=255)
                                            cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                          >  2:631181/1‑101 (MQ=255)
                                             gctgccccccGTCCTGCGGACTCCCCCCGGGATATTTAAGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcccc                                                         >  1:4311078/6‑101 (MQ=255)
                                              ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                        >  2:5462949/5‑101 (MQ=255)
                                                gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                      >  1:3123532/3‑101 (MQ=255)
                                                gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                      <  2:3067296/99‑1 (MQ=255)
                                                gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                      >  2:6782648/3‑101 (MQ=255)
                                                gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                      >  1:5712594/3‑101 (MQ=255)
                                                 ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                     <  2:6248872/100‑1 (MQ=255)
                                                     ccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAATTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAAcc                                                 >  1:7020026/1‑101 (MQ=255)
                                                      cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                <  2:1734063/101‑1 (MQ=255)
                                                       gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                               >  1:1797223/1‑101 (MQ=255)
                                                       gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                               <  2:3494386/101‑1 (MQ=255)
                                                           tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                           >  1:4394436/1‑101 (MQ=255)
                                                           tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                           >  1:131277/1‑101 (MQ=255)
                                                            gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                          <  2:5818121/101‑1 (MQ=255)
                                                             cGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGa                                         >  1:1036477/1‑101 (MQ=255)
                                                               gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAcc                                       <  2:3192070/101‑2 (MQ=255)
                                                               gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                       >  2:4121879/1‑101 (MQ=255)
                                                               gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                       <  2:661616/101‑1 (MQ=255)
                                                               gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACa                                       <  1:6947419/101‑1 (MQ=255)
                                                                aCTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                      >  1:5411843/1‑101 (MQ=255)
                                                                  tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                    >  1:6699167/1‑101 (MQ=255)
                                                                    cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                  <  1:3515730/101‑1 (MQ=255)
                                                                    cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                  <  2:3719405/101‑1 (MQ=255)
                                                                     ccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                 <  1:753740/101‑1 (MQ=255)
                                                                     ccccGGGATATTTAAGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATcc                                 <  1:5285122/101‑1 (MQ=255)
                                                                        cGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCcgcg                              >  2:4960481/1‑101 (MQ=255)
                                                                         gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                             <  1:6370916/101‑1 (MQ=255)
                                                                           gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                           <  2:648705/101‑1 (MQ=255)
                                                                             tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                         >  1:5847727/1‑101 (MQ=255)
                                                                               tttATGGCAAGATGAAGCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                       <  2:5124737/101‑1 (MQ=255)
                                                                               tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                       >  2:2958082/1‑101 (MQ=255)
                                                                               tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                       >  1:4471237/1‑101 (MQ=255)
                                                                               tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                       >  1:2769623/1‑101 (MQ=255)
                                                                               tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                       >  1:1561976/1‑101 (MQ=255)
                                                                                 tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                     >  1:1195099/1‑101 (MQ=255)
                                                                                    ggCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCg                  >  2:586815/1‑101 (MQ=255)
                                                                                       aaGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtg               >  1:1896496/1‑101 (MQ=255)
                                                                                         gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat             <  1:6519421/101‑1 (MQ=255)
                                                                                         gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat             >  1:4691007/1‑101 (MQ=255)
                                                                                         gATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCtgat             >  2:5764057/1‑101 (MQ=255)
                                                                                           tGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGCTGACAAGAACCGCAGCCCGGTTTTCGCttctga           >  2:913641/1‑96 (MQ=25)
                                                                                            gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg          <  2:1556099/101‑1 (MQ=255)
                                                                                             aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc         >  2:1290306/1‑101 (MQ=255)
                                                                                              aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa        <  1:1745171/101‑1 (MQ=255)
                                                                                               aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa       <  2:4824557/101‑1 (MQ=255)
                                                                                               aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa       <  1:6813387/101‑1 (MQ=255)
                                                                                               aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa       <  1:1006223/101‑1 (MQ=255)
                                                                                               aCTGC.GGCATTCANNTNGCCATNAATNTCNN.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa       <  1:1649412/101‑1 (MQ=255)
                                                                                                 tGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgc     >  1:385786/1‑101 (MQ=255)
                                                                                                     ggCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGcc  >  1:3777725/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCC  >  NC_014034/1734793‑1734992

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG