Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,907 A→C 1.4% intergenic (‑160/+33) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,9070A→C 1.4% 3.5 ‑2.8 ‑0.2365intergenic (‑160/+33)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (180/179):  total (186/179)
Fisher's exact test strand distribution p-value = 6.10e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.43e-01

Only 100 of 374 total aligned reads displayed.

TGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCG  >  NC_014034/1734808‑1735006
                                                                                                     |                                                                                                    
tGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCa                                                                                                      <  1:270172/101‑1 (MQ=255)
 gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                     >  2:946394/1‑101 (MQ=255)
 gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                     <  1:5127999/101‑1 (MQ=255)
 gCGGCAGCCGACGGGCCGGGCAGGGGGCCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCAt                                                                                                     <  2:6863564/101‑1 (MQ=17)
  cGTCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATa                                                                                                    >  1:2083950/1‑101 (MQ=255)
   gTCAGCCGANGGNNNGNGCAGGNNGGNGCNNCCCCCCNTCCTGCGGACTCCCNCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaa                                                                                                   <  1:1809698/101‑1 (MQ=18)
    tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                                  <  2:5126103/101‑1 (MQ=255)
    tCAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATaaa                                                                                                  <  1:5263220/101‑1 (MQ=255)
     cAGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAat                                                                                                 >  1:218161/1‑101 (MQ=255)
      aGCCGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATCAata                                                                                                >  2:3323076/1‑101 (MQ=255)
        ccGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATc                                                                                              <  2:5278855/101‑1 (MQ=255)
         cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                                             <  2:4751045/101‑1 (MQ=255)
         cGACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                                                                                             <  2:3640543/101‑1 (MQ=255)
          gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                            <  1:4328128/101‑1 (MQ=255)
          gACGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATccc                                                                                            <  2:1065517/101‑1 (MQ=255)
           gcgggccgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                          <  2:5106193/100‑1 (MQ=255)
           aCGGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                                                                                          >  1:59839/1‑101 (MQ=255)
                gcgggcAGCGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg                                                                                     <  2:309756/100‑1 (MQ=255)
                gacggacggggggCTCTG.CCCCCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTga                                                                                    >  2:4094258/8‑100 (MQ=2)
                 cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                                    <  1:898264/101‑1 (MQ=255)
                 cgggcAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGc                                                                                    <  2:1533982/101‑1 (MQ=255)
                     cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCgggg                                                                                >  1:3340748/1‑101 (MQ=255)
                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCGCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGAGCGGGGc                                                                               <  2:7081224/101‑1 (MQ=17)
                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.ggagatcggaag                                                                               >  1:4110351/1‑91 (MQ=14)
                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc                                                                               >  2:3595971/1‑101 (MQ=255)
                        gggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCag                                                                             <  1:657731/101‑1 (MQ=255)
                           ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGc                                                                          <  1:5336712/101‑1 (MQ=255)
                            gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc                                                                         <  2:4829791/101‑1 (MQ=255)
                             cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                                        >  2:631181/1‑101 (MQ=255)
                             cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGccc                                                                        <  1:4661582/101‑1 (MQ=255)
                               ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                      >  2:5462949/5‑101 (MQ=255)
                               ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                      <  2:5809800/97‑1 (MQ=255)
                               ctgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCg                                                                      >  1:4460420/5‑101 (MQ=255)
                                tgccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGGCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGc                                                                     >  1:4365400/4‑101 (MQ=255)
                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                                    >  2:6782648/3‑101 (MQ=255)
                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                                    >  1:5712594/3‑101 (MQ=255)
                                 gccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCt                                                                    >  1:3123532/3‑101 (MQ=255)
                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                                   <  1:4949540/100‑1 (MQ=255)
                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                                   >  1:3560767/2‑101 (MQ=255)
                                  ccccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                                   <  2:6248872/100‑1 (MQ=255)
                                  ccccccGTCCTGCGGACTCCCCCAGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTc                                                                   <  1:714805/100‑1 (MQ=255)
                                   cccccGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCa                                                                  <  2:884048/101‑1 (MQ=255)
                                       cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                              <  2:1734063/101‑1 (MQ=255)
                                       cGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCg                                                              <  1:4158889/101‑1 (MQ=255)
                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                             >  2:714239/1‑101 (MQ=255)
                                        gTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGa                                                             <  2:3494386/101‑1 (MQ=255)
                                          ccTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAACCGAAg                                                           >  2:5908243/1‑101 (MQ=255)
                                            tGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGAt                                                         >  1:131277/1‑101 (MQ=255)
                                             gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                                        <  1:4258372/101‑1 (MQ=255)
                                             gCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATg                                                        <  1:5874057/101‑1 (MQ=255)
                                               ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                                      >  1:6978798/1‑101 (MQ=255)
                                               ggACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAc                                                      <  2:6547320/101‑1 (MQ=255)
                                                gACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGAcc                                                     <  2:3192070/101‑2 (MQ=255)
                                                 gcTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACaa                                                    <  1:1151313/100‑1 (MQ=255)
                                                  cTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAg                                                   >  2:1952902/1‑101 (MQ=255)
                                                   tCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGa                                                  >  1:3848381/1‑101 (MQ=255)
                                                    ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                 <  1:7032337/101‑1 (MQ=255)
                                                    ccccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGAt                                                 <  1:1651951/101‑1 (MQ=255)
                                                     cccccGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATc                                                <  1:3515730/101‑1 (MQ=255)
                                                          gggATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCgg                                           <  1:6370916/101‑1 (MQ=255)
                                                            gATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGcc                                         <  2:648705/101‑1 (MQ=255)
                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                        >  2:4190326/1‑101 (MQ=255)
                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                        >  2:5619257/1‑101 (MQ=255)
                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                        >  2:2640025/1‑101 (MQ=255)
                                                             atatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGccc                                        <  2:2326730/101‑1 (MQ=255)
                                                              tatTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCg                                       <  2:1168137/101‑1 (MQ=255)
                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                     >  2:2958082/1‑101 (MQ=255)
                                                                tttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGt                                     >  1:6918291/1‑101 (MQ=255)
                                                                 ttATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGtt                                    >  2:5028464/1‑101 (MQ=255)
                                                                  tATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGttt                                   <  1:1328464/101‑1 (MQ=255)
                                                                      gCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGc                               >  1:3664817/1‑101 (MQ=255)
                                                                       cAAGATGAAACTGC.AGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCt                              <  1:2989964/101‑1 (MQ=255)
                                                                             gAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAg                        >  2:3856032/1‑101 (MQ=255)
                                                                              aaaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGc                       >  2:1290306/1‑101 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                      <  1:455220/101‑1 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                      >  1:1607354/1‑101 (MQ=255)
                                                                               aaCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGGGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCa                      <  1:1745171/101‑1 (MQ=255)
                                                                                aCTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCaa                     <  1:1006223/101‑1 (MQ=255)
                                                                                    c.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAgcgc                 <  2:2564536/101‑1 (MQ=255)
                                                                                        ccTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg              >  2:6623013/1‑101 (MQ=255)
                                                                                        ccTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCg              <  1:6783002/101‑1 (MQ=255)
                                                                                         cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGt             <  1:3937527/101‑1 (MQ=255)
                                                                                         cTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTCTTCGCTGATGAGCAAGCGCCCGt             >  1:4712259/1‑101 (MQ=255)
                                                                                          ttCATCTTGCGATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt            <  1:5463221/101‑1 (MQ=255)
                                                                                          ttCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtt            >  1:1073815/1‑101 (MQ=255)
                                                                                          ttCATCTTGCCATAAATATACC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGtc            >  2:2256656/1‑100 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc           <  2:69592/101‑1 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc           <  2:2758028/101‑1 (MQ=255)
                                                                                           tCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCAGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTc           >  1:6309592/1‑101 (MQ=255)
                                                                                             aTCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCgg         <  2:2054416/101‑1 (MQ=255)
                                                                                               cTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCa       >  1:852212/1‑101 (MQ=255)
                                                                                               cTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCa       >  2:3381840/1‑101 (MQ=255)
                                                                                                ttGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAg      <  1:1970181/101‑1 (MQ=255)
                                                                                                 tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg     >  2:601516/1‑101 (MQ=255)
                                                                                                 tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg     >  2:585085/1‑101 (MQ=255)
                                                                                                 tGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAgg     >  1:2481238/1‑101 (MQ=255)
                                                                                                  gCCTTAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCggagggc    >  2:4524836/1‑96 (MQ=255)
                                                                                                  gCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGt    >  1:2853735/1‑101 (MQ=255)
                                                                                                   ccATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc   >  1:1453865/1‑101 (MQ=255)
                                                                                                   ccATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTc   >  1:5825371/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTG.CCCCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAGATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCCCGCTCAACCGAAGATGACAAGATCCGCGGCCCGGTTTTCGCTGATGAGCAAGCGCCCGTTCGGCAGGTCG  >  NC_014034/1734808‑1735006

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG