Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,737,114 T→A 1.4% intergenic (+55/‑236) RCAP_rcc01606 → / → RCAP_rcc01607 tRNA/rRNA methyltransferase, SpoU family/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,737,1140T→A 1.4% 2.2 ‑2.0 ‑0.1286intergenic (+55/‑236)RCAP_rcc01606/RCAP_rcc01607tRNA/rRNA methyltransferase, SpoU family/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (157/123):  total (161/125)
Fisher's exact test strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.71e-01

Only 100 of 309 total aligned reads displayed.

GACGCTGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATC  >  NC_014034/1737014‑1737214
                                                                                                    |                                                                                                    
gACGCTGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCAt                                                                                                      >  2:249477/1‑101 (MQ=255)
 aCGCTGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATg                                                                                                     <  1:6257040/101‑1 (MQ=255)
 aCGCTGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATg                                                                                                     <  2:4723575/101‑1 (MQ=255)
  cGCTGCACGGGATGTTGNGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGa                                                                                                    <  2:1210979/101‑1 (MQ=255)
    cTGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAcc                                                                                                  <  1:6461826/101‑1 (MQ=255)
     tGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAccc                                                                                                 >  2:2109613/1‑100 (MQ=255)
      gCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAccgc                                                                                                >  1:3662799/1‑101 (MQ=255)
       cACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAccgcc                                                                                               >  1:5854794/1‑101 (MQ=255)
       cACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGAccgcc                                                                                               >  2:5056484/1‑101 (MQ=255)
          gggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCggg                                                                                            >  1:100898/1‑101 (MQ=255)
          gggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCggg                                                                                            >  1:1219971/1‑101 (MQ=255)
           ggATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCgggg                                                                                           <  1:4627116/101‑1 (MQ=255)
            gATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCggggg                                                                                          >  2:5541185/1‑101 (MQ=255)
               gTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGccc                                                                                       >  2:2289680/1‑101 (MQ=255)
                ttGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCa                                                                                      <  2:2965800/101‑1 (MQ=255)
                ttGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCa                                                                                      <  2:5371913/101‑1 (MQ=255)
                 tGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCaa                                                                                     >  1:1517000/1‑101 (MQ=255)
                 tGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCaa                                                                                     >  2:3554415/1‑101 (MQ=255)
                  gCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCaaa                                                                                    <  2:6659430/101‑1 (MQ=255)
                    ggCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAgg                                                                                  >  1:2110698/1‑101 (MQ=255)
                       aGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCAGGGGCCCAAAGGccc                                                                               <  2:4821090/101‑1 (MQ=255)
                        gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCt                                                                              <  2:4162465/101‑1 (MQ=255)
                        gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCt                                                                              >  2:4459043/1‑101 (MQ=255)
                        gCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCt                                                                              <  2:6302450/101‑1 (MQ=255)
                         cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTc                                                                             <  2:4086484/101‑1 (MQ=255)
                         cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTc                                                                             >  1:344265/1‑101 (MQ=255)
                         cTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTc                                                                             <  2:1344270/101‑1 (MQ=255)
                          ttttGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCg                                                                            >  1:4792455/1‑101 (MQ=255)
                           tttGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCgg                                                                           >  2:4231186/1‑101 (MQ=255)
                            ttGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGc                                                                          >  2:3463851/1‑101 (MQ=255)
                             tGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGcc                                                                         <  2:237192/101‑1 (MQ=255)
                               cgcCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCgcacag                                                                       >  2:154739/1‑96 (MQ=255)
                                 ccAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAgcg                                                                     >  2:498359/1‑101 (MQ=255)
                                    gCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGcgccc                                                                  <  1:4396356/101‑1 (MQ=255)
                                    gCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACCCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGcgccc                                                                  <  1:2568661/101‑1 (MQ=255)
                                     cGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGcgcccg                                                                 >  2:5879536/1‑101 (MQ=255)
                                        gATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCgcccgccc                                                              >  2:764457/1‑101 (MQ=255)
                                         aTTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                             <  2:5176761/101‑1 (MQ=255)
                                         aTTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                             <  1:3215761/101‑1 (MQ=255)
                                         aTTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccccccc                                                             >  1:2319192/1‑101 (MQ=255)
                                           tGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGcccgccccgc                                                           >  1:5485364/1‑101 (MQ=255)
                                              ggTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCa                                                        <  2:5677879/101‑1 (MQ=255)
                                               gTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                       >  1:1073500/1‑101 (MQ=255)
                                                  gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                                    >  1:5330340/1‑101 (MQ=255)
                                                  gACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggc                                                    <  2:6406342/101‑1 (MQ=255)
                                                   aCGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATggcg                                                   <  2:5141500/101‑1 (MQ=255)
                                                        tCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcgc                                              >  2:2691044/1‑101 (MQ=255)
                                                        tCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcgc                                              <  1:7057894/101‑1 (MQ=255)
                                                        tCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGgcgc                                              <  2:3662650/101‑1 (MQ=255)
                                                         cTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCt                                             >  2:4593262/1‑101 (MQ=255)
                                                          tGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCtt                                            >  2:3174159/1‑101 (MQ=255)
                                                           gACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtc                                           >  2:7055246/1‑101 (MQ=255)
                                                           gACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtc                                           >  1:2229238/1‑101 (MQ=255)
                                                            aCCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtct                                          <  1:956630/101‑1 (MQ=255)
                                                            aCCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtct                                          <  2:1675862/101‑1 (MQ=255)
                                                             ccGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctc                                         <  2:782602/101‑1 (MQ=255)
                                                               gAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgc                                       >  2:6484426/1‑101 (MQ=255)
                                                               gAGGACATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTtctccc                                       >  2:1140323/1‑99 (MQ=255)
                                                                aGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGGGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                      >  2:5245681/1‑101 (MQ=255)
                                                                aGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcc                                      >  2:4205094/1‑101 (MQ=255)
                                                                   ccATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCTTCTcgccccc                                   >  1:3273283/1‑99 (MQ=255)
                                                                   ccATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcccgc                                   >  1:3107590/1‑101 (MQ=255)
                                                                   ccATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTcgcccgc                                   >  2:1041585/1‑101 (MQ=255)
                                                                       cGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCcgg                               >  1:2876165/1‑101 (MQ=255)
                                                                        gCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCgggg                              >  2:5272752/1‑101 (MQ=255)
                                                                         ccGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGc                             <  1:4231381/101‑1 (MQ=255)
                                                                         ccGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGc                             >  1:6357534/1‑101 (MQ=255)
                                                                          cGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGcc                            >  1:5209128/1‑100 (MQ=255)
                                                                           gAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAg                           <  1:3038856/101‑1 (MQ=255)
                                                                           gAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCCGGGccg                           >  1:2671121/1‑99 (MQ=255)
                                                                              gTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGgcg                        >  1:1795997/1‑101 (MQ=255)
                                                                               tCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCCTCTCCCCCGCCCGGGCAGgcgc                       >  1:84927/1‑101 (MQ=18)
                                                                               tCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGGAGgggc                       >  1:6770331/1‑101 (MQ=255)
                                                                                cACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGcc                      <  2:1715254/101‑1 (MQ=255)
                                                                                 aCGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCg                     <  1:749270/101‑1 (MQ=255)
                                                                                   gcgcgACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGc                   >  2:6796296/1‑101 (MQ=255)
                                                                                     gcgACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGccc                 >  2:4311889/1‑100 (MQ=255)
                                                                                     gcgACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCct                 <  1:4139403/101‑1 (MQ=255)
                                                                                      cgACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctc                <  2:3800769/101‑1 (MQ=255)
                                                                                       gccATTGGCCCCATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct               <  1:5466520/99‑1 (MQ=255)
                                                                                       gACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct               <  2:3063231/101‑1 (MQ=255)
                                                                                       gACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCCCCGGGGCAGGCGCCGGCctct               >  1:3498320/1‑101 (MQ=255)
                                                                                         cATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGAGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGt             >  2:2042233/1‑101 (MQ=255)
                                                                                          aTTGGCCCCCTGCCCGCGGGGGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGtt            <  1:1713903/101‑1 (MQ=14)
                                                                                          aTTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGGGCCGGGCTCTGtt            >  1:2057547/1‑101 (MQ=255)
                                                                                          aTTGGCCCCAGGACCGCGGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGtt            <  2:1897450/101‑1 (MQ=255)
                                                                                           ttGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTg           >  1:2385653/1‑101 (MQ=255)
                                                                                            tGGCCCCATGCCAGCCGGGGGCCCAAAGGCCGCCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGGGTCCCGCCCCCCCGGGCCGGGGGCgggccggggggc          >  1:4009578/1‑91 (MQ=14)
                                                                                            tGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgc          <  1:5541475/101‑1 (MQ=255)
                                                                                            tGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCCCGCCCGCCGGGGGAGGCGCCCGGCTCTGTTgc          >  2:1112446/1‑101 (MQ=14)
                                                                                               ccccATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCt       >  2:6235995/1‑101 (MQ=255)
                                                                                                cccATGACCGCCGGGGGCCCAAGGGCCCTCGGCCGGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCtt      <  1:4090170/101‑1 (MQ=255)
                                                                                                cccATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCCCTGTTGCGCtt      >  2:3237339/1‑101 (MQ=255)
                                                                                                 ccATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTg     <  2:6039488/101‑1 (MQ=255)
                                                                                                 ccATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTg     <  1:6112850/101‑1 (MQ=255)
                                                                                                  cATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGa    <  1:3120686/101‑1 (MQ=255)
                                                                                                  cATGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGa    <  1:508942/101‑1 (MQ=255)
                                                                                                  cATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGa    <  1:7095541/101‑1 (MQ=255)
                                                                                                   aTGCCCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGAt   <  1:5995856/101‑1 (MQ=255)
                                                                                                    tGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATc  <  1:5724226/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GACGCTGCACGGGATGTTGCGGCAGCTTTTGCGCCAGCGGGATTGAGGTCGACGGATCTGACCGAGGCCATCGCCGAGGTCACGCGCGACATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCTTGATC  >  NC_014034/1737014‑1737214

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG