Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,769,372 C→G 94.8% P148P (CCC→CCG RCAP_rcc01636 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,769,3720C→G94.8% 19.9 ‑1.5 ‑0.1232P148P (CCC→CCGRCAP_rcc01636hypothetical protein
Reads supporting (aligned to +/- strand):  new base (82/138):  ref base (2/10):  total (84/148)
Fisher's exact test strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.45e-01

Only 100 of 237 total aligned reads displayed.

TCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.G.G..C.A.GGGGCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769284‑1769462
                                                                                                 |                                                                                            
tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCtt                                                                                   >  1:209334/1‑101 (MQ=255)
tCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCtt                                                                                   <  1:5506997/101‑1 (MQ=255)
 cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCttt                                                                                  >  2:248394/1‑101 (MQ=255)
 cGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCttt                                                                                  >  1:461470/1‑101 (MQ=255)
  ggCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTg                                                                                 >  1:5202888/1‑101 (MQ=255)
    cGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGcc                                                                               <  1:576569/101‑1 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCt                                                                              <  1:1082564/101‑1 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCt                                                                              >  2:6805330/1‑101 (MQ=255)
     gATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCt                                                                              <  2:5023647/101‑1 (MQ=255)
      aTCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGG.CGCG.CG.CGGGCTTTGCCtt                                                                             >  1:4958744/1‑101 (MQ=255)
       tCAGGACCAACTGGATGCGGTCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTg                                                                            <  2:441481/101‑1 (MQ=255)
        cAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTgg                                                                           >  1:4902777/1‑101 (MQ=255)
           gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        <  1:3032734/101‑1 (MQ=255)
           gACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGt                                                                        >  1:5642920/1‑101 (MQ=255)
            aCCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTc                                                                       <  1:182784/101‑1 (MQ=255)
             ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCTGCTTTGGGCGCGGTCGATGT.CGG.G..C.ATGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:6324624/101‑1 (MQ=255)
             ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      <  1:1143379/101‑1 (MQ=255)
             ccAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGGGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCg                                                                      >  1:2308008/1‑101 (MQ=255)
               aaCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAg                                                                    <  2:892049/101‑1 (MQ=255)
                aCTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAgg                                                                   <  2:3989247/101‑1 (MQ=255)
                 cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:5353239/101‑1 (MQ=255)
                 cTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgc                                                                  <  2:3363145/101‑1 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 <  2:461458/101‑1 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcg                                                                 >  2:3303608/1‑101 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGgcg                                                                 <  1:1315219/101‑1 (MQ=255)
                  tGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGgcg                                                                 <  1:2746196/101‑1 (MQ=255)
                   ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  2:110459/1‑101 (MQ=255)
                   ggATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGgcgc                                                                >  2:6118429/1‑101 (MQ=255)
                    gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               >  2:6357256/1‑101 (MQ=255)
                    gATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGGGGT.CGGG.CG.CGGGCTTTGCCTGGGGGGGGAGGCGCt                                                               >  2:6278072/1‑101 (MQ=255)
                    gATGCCGGCCGCGCGGCGGAAATCGTGCCGGCTTGGGGCGCGGTCGCTGT.T.G.GT.CTA.GGGGCGGTGGTGG.CG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCt                                                               <  2:5827073/101‑1 (MQ=255)
                      tGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTc                                                             <  2:3682703/101‑1 (MQ=255)
                       gCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTcc                                                            <  1:4713203/101‑1 (MQ=255)
                        cGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCg                                                           >  1:3586525/1‑101 (MQ=255)
                         ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTGGATGTGG.G.G..C.AGGGGGCGGGGGTGG.GG.CG.CGGGCGTTGCCTTGGGGTGGGGGCGCTTACgg                                                          >  2:2274655/1‑101 (MQ=255)
                         ggCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGCGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCgg                                                          <  2:4707636/101‑1 (MQ=255)
                          gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         >  2:1715830/1‑101 (MQ=255)
                          gCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCggg                                                         <  1:1959084/101‑1 (MQ=255)
                           cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  1:2835330/101‑1 (MQ=255)
                           cccGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGc                                                        <  2:6735412/101‑1 (MQ=255)
                             cgcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAc                                                      <  1:850069/101‑1 (MQ=255)
                              gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     >  1:5818901/1‑101 (MQ=255)
                              gcgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCAcc                                                     <  1:605508/101‑1 (MQ=255)
                               cgcgGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCg                                                    <  2:2950947/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  <  2:3478290/101‑1 (MQ=255)
                                 cggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGAt                                                  >  1:2609630/1‑101 (MQ=255)
                                  ggcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATg                                                 <  1:6203143/101‑1 (MQ=255)
                                   gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:5220201/101‑1 (MQ=255)
                                   gcggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGCTGGT.CGCG.CG.CGGGCTTTGCATTGGGGTCGAGGCGCTTCCGGGCACCGATgc                                                <  1:4642168/101‑1 (MQ=255)
                                    cggAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATgcg                                               >  2:6440243/1‑101 (MQ=255)
                                      gAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCt                                             >  2:4263799/1‑101 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            >  1:6547788/1‑101 (MQ=255)
                                       aaaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTc                                            <  1:1842029/101‑1 (MQ=255)
                                        aaTCGTCGCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTcc                                           >  1:5944218/1‑101 (MQ=255)
                                        aaTCGGCGCGGCTTTGGGCGCGGTCGATGG.C.G.GGCC.G.GGGGCGGTGGC.CGCG.CC.CGGGCTTTGCCTTGGGGTGGGGGCGCTTCCTGGCACCGACGCGATcc                                           >  2:5417886/1‑101 (MQ=255)
                                          tcgtcgCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCtt                                         <  1:4264021/101‑1 (MQ=255)
                                           cgtcgCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        <  2:4501883/101‑1 (MQ=255)
                                           cgtcgCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTg                                        >  2:547260/1‑101 (MQ=255)
                                             tcgCGGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAt                                      <  1:379252/101‑1 (MQ=255)
                                              cgcgGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     >  2:1038665/1‑101 (MQ=255)
                                              cgcgGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAtt                                     <  1:3964775/101‑1 (MQ=255)
                                              cgcgGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGGCGAGGCGGTTCCGGGCACCGATGCGCTCCTTGagt                                     >  1:6940783/1‑99 (MQ=255)
                                               gcgGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGAttt                                    <  1:1427367/101‑1 (MQ=255)
                                                cgGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:5394685/101‑1 (MQ=255)
                                                cgGCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtc                                   <  1:2342305/101‑1 (MQ=255)
                                                cgGCTGTGGGCGGGGTCGATGT.C.GCG..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGGTCCTTGATTtc                                   <  2:334741/101‑1 (MQ=255)
                                                 ggCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  >  1:1021364/1‑101 (MQ=255)
                                                 ggCTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcg                                  <  2:2180194/101‑1 (MQ=255)
                                                   cTTTGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTtcgtc                                <  1:3318012/101‑1 (MQ=255)
                                                     ttGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              <  2:5943914/101‑1 (MQ=255)
                                                     ttGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgc                              <  2:4879480/101‑1 (MQ=255)
                                                      tGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             <  2:1939592/101‑1 (MQ=255)
                                                      tGGGCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgcc                             >  1:3192383/1‑101 (MQ=255)
                                                       gggCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccg                            <  2:5805492/101‑1 (MQ=255)
                                                        ggCGCGGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTcgccgc                           <  2:2117929/101‑1 (MQ=255)
                                                          cgcgGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGACTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCgcgc                         <  1:7071285/101‑1 (MQ=255)
                                                           gcgGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  1:4059868/1‑101 (MQ=255)
                                                           gcgGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCa                        >  1:4161833/1‑101 (MQ=255)
                                                            cgGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:2368097/101‑1 (MQ=255)
                                                            cgGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  2:5636445/101‑1 (MQ=255)
                                                            cgGTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCAt                       <  1:6405660/101‑1 (MQ=255)
                                                             ggTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      <  2:1766191/101‑1 (MQ=255)
                                                             ggTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATc                      >  1:1779663/1‑101 (MQ=255)
                                                              gTCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcg                     <  2:5169604/101‑1 (MQ=255)
                                                               tCGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgc                    <  2:1023745/101‑1 (MQ=255)
                                                                cGATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATcgcg                   <  2:1583049/101‑1 (MQ=255)
                                                                 gATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  1:6852351/1‑101 (MQ=255)
                                                                 gATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  >  1:2495929/1‑101 (MQ=255)
                                                                 gATGT.CGG.G..C.AGGGGGCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCgg                  <  2:3090627/101‑1 (MQ=255)
                                                                          tcgggcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTg              <  2:2444349/93‑1 (MQ=255)
                                                                            gggcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGt            <  2:2617825/95‑1 (MQ=255)
                                                                              gcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          >  1:6706309/5‑101 (MQ=255)
                                                                              gcagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgc          <  2:4066790/97‑1 (MQ=255)
                                                                               cagggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         <  1:613519/98‑1 (MQ=255)
                                                                               cagggggCGGTGGT.C.GCGCG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgcc         >  1:3392724/4‑101 (MQ=255)
                                                                                agggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccg        <  2:6570033/99‑1 (MQ=255)
                                                                                 gggggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccga       <  2:750452/100‑1 (MQ=255)
                                                                                    ggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  2:1448060/101‑1 (MQ=255)
                                                                                    ggCGGTGGT.CGCG.CG.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    <  2:3829362/101‑1 (MQ=255)
                                                                                    ggCGGTGGT.C.GC.GCGCGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGtgccgatgc    >  1:1350242/1‑101 (MQ=255)
                                                                                                 |                                                                                            
TCGGCGATCAGGACCAACTGGATGCGGCCCGCGCGGCGGAAATCGTCGCGGCTTTGGGCGCGGTCGATGT.C.G.G..C.A.GGGGCGGTGGT.C.CC.CC.CGGGCTTTGCCTTGGGGTCGAGGCGCTTCCGGGCACCGATGCGCTCCTTGATTTCGTCGCCGCGCATCGCGGGCTGGTGCCGATGCCG  >  NC_014034/1769284‑1769462

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG