Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,782,184 T→A 69.6% S661T (TCA→ATA)  RCAP_rcc01644 → FMN‑binding domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,782,1840T→A69.6% 0.0 ‑2.6 ‑0.0290S661T (TCA→ACA) RCAP_rcc01644FMN‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (102/99):  ref base (55/33):  total (157/133)
Fisher's exact test strand distribution p-value = 7.30e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

Only 100 of 293 total aligned reads displayed.

GCTGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782093‑1782275
                                                                                             |                                                                                            
  tgtgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATgacgac                                                                                   <  2:4846583/101‑1 (MQ=255)
    tgCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACaa                                                                                 <  1:5378363/101‑1 (MQ=255)
     gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACaaa                                                                                <  1:1033350/101‑1 (MQ=255)
     gCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACaaa                                                                                >  1:2079964/1‑101 (MQ=255)
      ccGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACaaaa                                                                               <  1:5008524/101‑1 (MQ=255)
        gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGc                                                                             <  1:4745801/101‑1 (MQ=255)
        gcgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGc                                                                             >  1:2498828/1‑101 (MQ=255)
        agcggcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGc                                                                             >  2:5735231/5‑101 (MQ=255)
         cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGcc                                                                            <  1:198417/101‑1 (MQ=255)
         cgTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGcc                                                                            >  1:179414/1‑101 (MQ=255)
          tccgatctcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCg                                                                           <  1:2827148/93‑1 (MQ=255)
          gTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCg                                                                           >  2:6844498/1‑101 (MQ=255)
            gcgctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCt                                                                         >  1:4067851/1‑101 (MQ=255)
              gctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCtgt                                                                       <  2:6385474/101‑1 (MQ=255)
              gctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCtgt                                                                       <  1:5326218/101‑1 (MQ=255)
              cgtgcccCTAGGGCGGGATCAACGGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCtgt                                                                       <  2:2299172/99‑1 (MQ=255)
               ctgcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCtgtg                                                                      <  2:1767801/101‑1 (MQ=255)
                 gcgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCtgtgtg                                                                    <  2:469898/101‑1 (MQ=255)
                  cgcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCgagatcgga                                                                   >  2:2827046/1‑93 (MQ=255)
                   gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGcc                                                                  <  2:1514833/101‑1 (MQ=255)
                   gcCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGcc                                                                  <  1:869329/101‑1 (MQ=255)
                   gattATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGcc                                                                  <  2:915905/98‑1 (MQ=255)
                    ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  2:3055096/101‑1 (MQ=255)
                    ccTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCt                                                                 <  1:6572221/101‑1 (MQ=255)
                     cTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCtt                                                                <  1:4604923/101‑1 (MQ=255)
                      tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  2:3801788/101‑1 (MQ=255)
                      tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               >  2:3788622/1‑101 (MQ=255)
                      tATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAG.GCCTTTGCTGTGTGGGGG.TCCCTTGACACCAAAACCCCCtggggcccttg                                                               >  2:5362055/1‑92 (MQ=255)
                      caTGGCGCGATCACGCGCTCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCttt                                                               <  1:3828781/100‑1 (MQ=255)
                       aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              <  2:6590609/101‑1 (MQ=255)
                       aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              <  1:3901694/101‑1 (MQ=255)
                       aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTg                                                              >  2:3684115/1‑101 (MQ=255)
                       aTGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGGGTTTCCAGTGCCTTGACTGTGT.GGCG.ATACATGACGACAAAAGAAGCTGTGGGCCTTTg                                                              >  1:1296549/1‑101 (MQ=255)
                        tGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTgg                                                             <  1:2187393/101‑1 (MQ=255)
                        tGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTgg                                                             >  2:716688/1‑101 (MQ=255)
                             cgATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTg                                                        >  2:3924355/1‑101 (MQ=255)
                              gATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTgg                                                       >  1:483508/1‑101 (MQ=255)
                                tCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcg                                                     >  2:6913421/1‑101 (MQ=255)
                                 cAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTggcgg                                                    >  2:5823292/1‑101 (MQ=255)
                                     cgccgcGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               <  1:6459106/96‑1 (MQ=255)
                                     cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               >  2:4389065/1‑101 (MQ=255)
                                     cgcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aa                                               <  1:4511457/101‑1 (MQ=255)
                                      gcACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaa                                              >  2:1930896/1‑100 (MQ=255)
                                       ccgatctgagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaac                                             <  2:6088889/94‑3 (MQ=255)
                                        aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            <  1:5914632/101‑4 (MQ=255)
                                        aCCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacg                                            <  2:2498734/101‑4 (MQ=255)
                                          cGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacgaa                                          >  1:1391983/1‑96 (MQ=255)
                                           ggCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGaaa                                          <  2:7086708/101‑1 (MQ=255)
                                            gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         >  1:6806044/1‑101 (MQ=255)
                                            gCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAg                                         >  2:1711926/1‑101 (MQ=255)
                                             cGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAgg                                        <  1:6686174/101‑1 (MQ=255)
                                              gagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGc                                       >  1:2929715/1‑101 (MQ=255)
                                              gagaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGcg.aaacagatcgg                                      >  1:6089201/1‑92 (MQ=255)
                                               agaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCt                                      <  2:3558114/101‑1 (MQ=255)
                                                gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     >  1:5232031/1‑101 (MQ=255)
                                                gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     >  2:1915455/1‑101 (MQ=255)
                                                gaTCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTc                                     <  2:2873354/101‑1 (MQ=255)
                                                   ccGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggg                                  >  2:1175833/1‑101 (MQ=255)
                                                    cGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGc                                 <  2:240633/101‑1 (MQ=255)
                                                      cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGCCGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCggtccg                               >  1:2288786/1‑97 (MQ=255)
                                                      cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               >  1:5854273/1‑101 (MQ=255)
                                                      cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               >  2:2189951/1‑101 (MQ=255)
                                                      cTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCAt                               <  2:4601314/101‑1 (MQ=255)
                                                       tATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              >  1:3892997/1‑101 (MQ=255)
                                                       tATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATg                              <  1:716708/101‑1 (MQ=255)
                                                         ttCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  1:1924190/101‑1 (MQ=255)
                                                         ttCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGaa                            <  2:1076244/101‑1 (MQ=255)
                                                          tCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           >  1:2988767/1‑101 (MQ=255)
                                                          tCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAg                           >  2:2991712/1‑101 (MQ=255)
                                                            cGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcg                         >  2:2457338/1‑101 (MQ=255)
                                                             gAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  1:4677384/1‑101 (MQ=255)
                                                             gAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcgg                        >  2:4298582/1‑101 (MQ=255)
                                                               gtgtTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcg                      >  1:5437742/1‑101 (MQ=255)
                                                               gtgtTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCAGGCATGAAgcggcg                      >  1:3657911/1‑101 (MQ=255)
                                                                tgtTTCCAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcgg                     >  1:6512276/1‑101 (MQ=255)
                                                                 gtTTCCAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAgcggcggc                    >  2:6697624/1‑101 (MQ=255)
                                                                     ccAGTGCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                >  2:2604403/1‑101 (MQ=255)
                                                                     ccAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGtt                <  1:3709328/101‑1 (MQ=255)
                                                                      cAGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGttt               <  1:6383741/101‑1 (MQ=255)
                                                                       aGTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTc              <  1:4389287/101‑1 (MQ=255)
                                                                        gggCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  2:27029/99‑1 (MQ=255)
                                                                        gTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTcg             <  2:4152787/101‑2 (MQ=255)
                                                                        gTGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCt             <  2:5957461/101‑1 (MQ=255)
                                                                         tGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            <  1:2697237/101‑1 (MQ=255)
                                                                         tGCCTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTg            <  1:3502906/101‑1 (MQ=255)
                                                                          gCCTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGc           >  2:6978373/1‑101 (MQ=255)
                                                                            cTTGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAg         >  1:561176/1‑101 (MQ=255)
                                                                            cTTGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAg         >  2:6297945/1‑101 (MQ=255)
                                                                             ttGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGa        >  2:5590706/1‑101 (MQ=255)
                                                                              tGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGGTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:3408493/1‑101 (MQ=255)
                                                                              tGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  2:2109000/1‑101 (MQ=255)
                                                                              tGACTGTGT.GGCGATC.CATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       >  1:5121987/1‑101 (MQ=255)
                                                                              tGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       <  2:135518/101‑1 (MQ=255)
                                                                              tGACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGAt       <  1:6913775/101‑1 (MQ=255)
                                                                               gACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      >  1:1729427/1‑101 (MQ=255)
                                                                               gACTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATc      >  2:3479974/1‑101 (MQ=255)
                                                                                aCTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCg     >  1:1244297/1‑101 (MQ=255)
                                                                                 cTGTGT.GGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGt    >  2:5147983/1‑101 (MQ=255)
                                                                                  tgtgt.gGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGCAGATCGTg   >  2:5466126/1‑101 (MQ=255)
                                                                                   gtgt.gGCG.ATCCATGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCGAAACGAAAGGCTCGGGCATGAAGCGGCGGCGGGTTCTGCAGATCGTgg  >  2:2940731/1‑101 (MQ=255)
                                                                                             |                                                                                            
GCTGTGCCGCGTGCGCTGCGCCTATGGCGCGATCAAGCGCACCGGCGAGATCCGCTATTCCGAGTGTTTCCAGTGCCTTGACTGTGT.GGCG.TCACTTGACGACAAAAGCCGCTGTGTGCCTTTGGTGCTGGCGGCG.AACGAAAGGCTCGGGCATGAAGCGGCGGCGGTTTCTGCAGATCGTGG  >  NC_014034/1782093‑1782275

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG