Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,786,541 C→T 4.1% A225A (GCC→GCT RCAP_rcc01648 → ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,786,5410C→T 4.1% 22.5 0.0 ‑0.0314A225A (GCC→GCTRCAP_rcc01648ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (6/7):  ref base (139/161):  total (146/168)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 330 total aligned reads displayed.

TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641
                                                                                                   |                                                                                                    
tGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTtt                                                                                                    <  2:4906190/101‑1 (MQ=255)
 gATGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGGCTttc                                                                                                   >  1:4755035/1‑100 (MQ=255)
  tcgccctaTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCg                                                                                                 <  1:3332066/94‑1 (MQ=255)
   tGCCGCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGt                                                                                                <  1:2709776/101‑1 (MQ=255)
     gcGCATCTGGTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTAt                                                                                              <  1:4415407/100‑1 (MQ=255)
       gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                            <  1:461970/101‑1 (MQ=255)
       gCATCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATcc                                                                                            <  1:4183747/101‑1 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCag                                                                                          >  1:3361451/1‑100 (MQ=255)
         aTCTGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCAt                                                                                          <  2:6819384/101‑1 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       >  1:6725475/1‑101 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       <  1:3085773/101‑1 (MQ=255)
            tGATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTg                                                                                       <  2:5396404/101‑1 (MQ=255)
             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      <  2:1917269/101‑1 (MQ=255)
             gATCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGa                                                                                      >  1:6888144/1‑101 (MQ=255)
              aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                     <  2:5683310/101‑1 (MQ=255)
              aTCGCCGCGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGaa                                                                                     <  2:5001094/101‑1 (MQ=255)
                 gccgcGGCGCGGGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGCAttt                                                                                  >  2:1269994/1‑101 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                <  2:5757006/101‑1 (MQ=255)
                   cgcggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTcc                                                                                <  1:130959/101‑1 (MQ=255)
                      ggcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGa                                                                             >  1:1689173/1‑101 (MQ=255)
                       gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                            <  1:2023476/101‑1 (MQ=255)
                       gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                            <  2:2595260/101‑1 (MQ=255)
                       gcgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGAt                                                                            <  1:4247262/101‑1 (MQ=255)
                        cgcggGCGGGGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATg                                                                           <  2:823376/101‑1 (MQ=255)
                          cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                         <  1:1580473/101‑1 (MQ=255)
                          cgggcgggGCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGtt                                                                         >  1:5921999/1‑101 (MQ=255)
                           gggcggggCTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTc                                                                        <  1:6287050/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  2:3499793/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  1:964769/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCg                                                                       <  2:3145375/101‑1 (MQ=255)
                            ggcggggcTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.ATTTCGTATCCATCTGAATTTCCAGCTGTTcc                                                                       >  1:6615882/1‑100 (MQ=255)
                              gggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  2:4425609/95‑1 (MQ=255)
                              cggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  2:4000255/95‑1 (MQ=255)
                              cggggctttCGCTGATATGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  1:6416194/95‑1 (MQ=255)
                              cgatcttttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGAc                                                                     <  1:214641/95‑1 (MQ=255)
                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    <  2:207784/96‑1 (MQ=255)
                               ggggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACa                                                                    >  1:499665/6‑101 (MQ=255)
                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   <  2:1698855/97‑1 (MQ=255)
                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   <  2:6100539/97‑1 (MQ=255)
                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   <  2:708122/97‑1 (MQ=255)
                                gggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAt                                                                   >  2:4557173/5‑101 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:281848/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:3030153/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  2:3055580/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:3995992/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACAtt                                                                  <  1:5756109/98‑1 (MQ=255)
                                 ggctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTGCGACAtt                                                                  >  2:53091/4‑101 (MQ=255)
                                  gctttCGCTGATCTGGAAGGTCGTGCTGGTTGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTg                                                                 <  2:746779/99‑1 (MQ=255)
                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                <  2:59300/100‑1 (MQ=255)
                                   ctttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGc                                                                >  2:4341003/2‑101 (MQ=255)
                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACagatcg                                                               >  2:214638/1‑96 (MQ=255)
                                    tttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGcc                                                               <  1:4500737/101‑1 (MQ=255)
                                     ttCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCg                                                              <  1:1637580/101‑1 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            <  2:4468811/101‑1 (MQ=255)
                                       cGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAg                                                            <  2:3315800/101‑1 (MQ=255)
                                           gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                        >  1:4716471/1‑101 (MQ=255)
                                           gATCTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGc                                                        >  1:4272519/1‑101 (MQ=255)
                                              cTGGAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCg                                                     >  1:4799984/1‑101 (MQ=255)
                                                ggAAGGTCGTGCTGGTGGTCGAATTTCTGGG.CCTCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGcc                                                   >  1:1062375/1‑101 (MQ=255)
                                                  aaGGTCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTg                                                 >  2:3529018/1‑101 (MQ=255)
                                                     gTCGTGATGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggc                                              >  2:7024460/1‑101 (MQ=255)
                                                      tcttGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcg                                             <  2:4220198/98‑1 (MQ=255)
                                                      tCGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcg                                             <  1:6151188/101‑1 (MQ=255)
                                                       cGTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAAGGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcgg                                            >  1:3718279/1‑101 (MQ=255)
                                                        gTGCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggc                                           <  2:3734952/101‑1 (MQ=255)
                                                          gCTGGTGGTCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGggcggcgg                                         >  1:5984082/1‑101 (MQ=255)
                                                             ggtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCtt                                      <  2:1741835/101‑1 (MQ=255)
                                                             ggtggtCGAATTTCT.GGTCCGCTCAAACGGGATCGG.CTTTCGTATCAATCTGAATTTCCAGATGTTCGTCATTACCCAGGCGCTCGCCTGGGCCGCGGCtt                                      >  2:3398542/1‑101 (MQ=255)
                                                              gtggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCttt                                     <  2:1807425/101‑1 (MQ=255)
                                                                ggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                                   >  1:5680845/1‑101 (MQ=255)
                                                                ggtCGAATTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCg                                   >  1:1014696/1‑101 (MQ=255)
                                                                     aaTTTCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGc                              <  1:1930393/101‑1 (MQ=255)
                                                                      atctctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCg                             <  2:2255894/94‑1 (MQ=255)
                                                                       tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGt                            <  2:1394898/101‑1 (MQ=255)
                                                                       tttCTGGG.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCCGCTTTCGTCTGCGt                            >  2:3758696/1‑101 (MQ=255)
                                                                        ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                           >  2:4252154/6‑101 (MQ=255)
                                                                        ttctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTg                           >  2:2702687/6‑101 (MQ=255)
                                                                          ctggg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGAt                         >  2:6326984/4‑101 (MQ=255)
                                                                             gg.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCt                      <  2:6725133/101‑1 (MQ=255)
                                                                              g.CCGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTc                     <  1:1839436/101‑1 (MQ=255)
                                                                                 cGCTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGc                   <  2:2837591/101‑1 (MQ=255)
                                                                                   cTCGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCg                 <  2:5177805/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               <  2:2419422/101‑1 (MQ=255)
                                                                                     cGAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTc               >  2:5599625/1‑101 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  2:892931/1‑101 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  1:2344487/1‑101 (MQ=255)
                                                                                      gAACGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCg              >  2:6213876/1‑101 (MQ=255)
                                                                                         cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt           >  1:5369502/1‑101 (MQ=255)
                                                                                         cGGGATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAAt           >  1:5890801/1‑101 (MQ=255)
                                                                                          gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTTCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          >  1:1168990/1‑101 (MQ=255)
                                                                                          gggATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATg          >  1:6897503/1‑101 (MQ=255)
                                                                                            gATCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGc        <  1:3206842/101‑1 (MQ=255)
                                                                                             aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       <  2:1014661/101‑1 (MQ=255)
                                                                                             aTCGG.CTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGAGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCt       >  2:1491963/1‑101 (MQ=255)
                                                                                               tcggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTg      >  1:4927154/5‑101 (MQ=255)
                                                                                                cggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGa     <  1:7046417/98‑1 (MQ=255)
                                                                                                 ggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTgct    >  2:6888748/3‑99 (MQ=255)
                                                                                                 ggcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGAt    >  2:6899100/3‑101 (MQ=255)
                                                                                                  gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  1:3446609/2‑101 (MQ=255)
                                                                                                  gcTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATc   >  2:4716265/2‑101 (MQ=255)
                                                                                                   |                                                                                                    
TGATGCCGCATCTGATCGCCGCGGCGCGGGCGGGCGTTTCGCTGATCTGGAAGGTCGTGCTGGTGGTCGAATTTCT.GG.CCGCTCGAACGGGATCGGCCTTTCGTATCCATCTGAATTTCCAGATGTTCGACATTGCCGAGGTGCTCGCCTGGGCGGCGGCTTTCGTCTGCGTGATGCTCGCCGTCGAATGGCTGATCC  >  NC_014034/1786444‑1786641

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG