Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,787,341 T→A 1.5% intergenic (+66/+161) RCAP_rcc01649 → / ← RCAP_rcc01650 ABC transporter ATP‑binding protein/ABC transporter permease

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,787,3410T→A 1.5% 3.0 ‑2.0 ‑0.3274intergenic (+66/+161)RCAP_rcc01649/RCAP_rcc01650ABC transporter ATP‑binding protein/ABC transporter permease
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (153/117):  total (157/117)
Fisher's exact test strand distribution p-value = 1.38e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.54e-01

Only 100 of 308 total aligned reads displayed.

TGACGGGCCGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGCCTTTGGGGC  >  NC_014034/1787241‑1787440
                                                                                                     |                                                                                                       
 gACGGGCCGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATg                                                                                                        >  2:2400401/1‑101 (MQ=255)
  aCGGGCCGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGa                                                                                                       >  2:7072256/1‑101 (MQ=255)
   cGGGCCGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGAc                                                                                                      <  2:6150036/101‑1 (MQ=255)
      gCCGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGt                                                                                                   <  2:6979075/101‑1 (MQ=255)
       ccGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGtt                                                                                                  <  2:4798173/101‑1 (MQ=255)
        cGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGttt                                                                                                 <  1:5487775/101‑1 (MQ=255)
           ccGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCg                                                                                              >  1:5204758/1‑101 (MQ=255)
            cGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGc                                                                                             <  2:3095212/101‑1 (MQ=255)
            cGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGc                                                                                             <  1:1913940/101‑1 (MQ=255)
            cGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGc                                                                                             >  1:4079916/1‑101 (MQ=255)
            cGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGc                                                                                             >  1:7069403/1‑101 (MQ=255)
             ggCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCa                                                                                            >  2:4021121/1‑101 (MQ=255)
               cgcgGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAg                                                                                          <  2:5142072/101‑1 (MQ=255)
                gcgGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGc                                                                                         <  1:2048465/101‑1 (MQ=255)
                  ggCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCgg                                                                                       >  2:3409004/1‑101 (MQ=255)
                   gCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCggg                                                                                      >  1:613020/1‑101 (MQ=255)
                    cggAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGa                                                                                     <  2:2679104/99‑1 (MQ=255)
                     tGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGAc                                                                                    >  2:5636047/1‑101 (MQ=255)
                     tGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGAc                                                                                    >  1:1380298/1‑101 (MQ=255)
                      gAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACa                                                                                   <  1:4788241/101‑1 (MQ=255)
                      gAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACa                                                                                   <  1:3353478/101‑1 (MQ=255)
                      gAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACa                                                                                   <  2:3750822/101‑1 (MQ=255)
                      gAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACa                                                                                   >  2:4551745/1‑101 (MQ=255)
                      gAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACa                                                                                   >  2:5370882/1‑101 (MQ=255)
                       aGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACag                                                                                  >  2:1211027/1‑101 (MQ=255)
                       aGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACag                                                                                  >  1:6551130/1‑101 (MQ=255)
                       aGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACag                                                                                  <  1:1051415/101‑1 (MQ=255)
                        gCGAGGGGGTAGCGCGGTTTCGGGCGGATCAGGAAAGCCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACaga                                                                                 <  1:1725359/101‑1 (MQ=255)
                         ggAGGGGTGCGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACagag                                                                                <  1:1162932/100‑1 (MQ=255)
                          gAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAgg                                                                               <  2:2333606/101‑1 (MQ=255)
                          gAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAgg                                                                               >  2:1366314/1‑101 (MQ=255)
                          gAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAgg                                                                               <  1:5724574/101‑1 (MQ=255)
                           aGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGTCATGACCGTTTCCGCAAGCAGCAAagagcc                                                                              >  2:891389/1‑99 (MQ=18)
                           aGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGc                                                                              >  2:3988196/1‑101 (MQ=255)
                            gggttggacgcgCTCTCGGGCGGATCAGGAAAGGCCCCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGcc                                                                             <  2:6060414/93‑1 (MQ=18)
                            ggggTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGcc                                                                             >  1:3755821/1‑101 (MQ=255)
                             gggTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.t                                                                           <  1:2126575/101‑1 (MQ=255)
                              ggTCAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCTGCAAGCGGGACAGAGGCC.Tg                                                                          >  1:5611820/1‑101 (MQ=255)
                                tgtgcggtgTTTCGGGTGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTCCCGCAAGCGGGACAGAGGCC.Tgcg                                                                        <  2:5965557/93‑1 (MQ=18)
                                tGAGCGCGGTTTCGGGCGGATCAGGAAAGGCCCCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.Tgcg                                                                        <  1:3422001/101‑1 (MQ=255)
                                      cgGTTTCGGGCGGATCAGGAAAGGCCCCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGc                                                                  <  1:1633837/101‑1 (MQ=255)
                                        gTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGccc                                                                <  1:5282449/101‑1 (MQ=255)
                                         tttCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGccc.c                                                              <  1:6218302/101‑1 (MQ=255)
                                         tttCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGccc.c                                                              >  1:689517/1‑101 (MQ=255)
                                         tttCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGccc.c                                                              <  1:181961/101‑1 (MQ=255)
                                         gttCGGGCGGATCAGGAAAGGCCCCGCCTTCCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGTCAGAGGCC.TGCGCTCCGccc.c                                                              <  2:1824076/100‑1 (MQ=255)
                                          ttCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.cg                                                             >  2:6785752/1‑101 (MQ=255)
                                          tccGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.cg                                                             >  1:4262310/3‑101 (MQ=255)
                                             gggCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.cggcg                                                          >  1:3068550/1‑101 (MQ=255)
                                              ggCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCCAGCGGGACAGATGCC.TGCGCTCCCCCC.cggcgg                                                         >  1:565795/1‑101 (MQ=255)
                                                cGGATCGGGAAAGGCACTGCCTTTCCCCGCCCGCTGGCGCATCACCTGTGCCAAGACCGTTTCCCCAAGCACGACAGAGGCC.TTTTCCCCGccc.cccccgc                                                        >  1:6678489/1‑95 (MQ=25)
                                                cGGATCGGGAAAGGCACTGCCTTTCCCCGCCCGCTGGCGCATCACCTGTGCCAAGACCGTTTCCCCAAGCACGACAGAGGCC.TTTGCCCCCCCC.Ccgccgc                                                        >  1:471374/1‑101 (MQ=25)
                                                 ggATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGgcgc                                                      >  2:1085122/1‑101 (MQ=255)
                                                 ggATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGgcgc                                                      >  1:3144484/1‑101 (MQ=255)
                                                 ggATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGgcgc                                                      >  2:499910/1‑101 (MQ=255)
                                                  gATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGcgcg                                                     >  2:6337860/1‑101 (MQ=255)
                                                    ccAGGAAAGCCCCCGCCTTCCCCCGCCCG.TAGCGCAGCAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGaa                                                   <  1:6311467/100‑1 (MQ=18)
                                                     cAGGAAAGGCCCCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAAt                                                  <  1:4857033/101‑1 (MQ=255)
                                                     cAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCAGCCC.CGGCGGCGCGAAt                                                  <  1:2400495/101‑1 (MQ=255)
                                                      aGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATg                                                 <  1:35252/101‑1 (MQ=255)
                                                       ggAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATgc                                                >  1:3970259/1‑101 (MQ=255)
                                                        gAAAGGCACCGCCTTCCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATgcg                                               <  2:2985618/101‑1 (MQ=255)
                                                         aaaGGCACTGCCTTTCCCCGCCCGCTGGCGCATCACCTGTGCCAAGACCGTTTCCCCAAGCACGACAGAGGCCTTTCGCCCCGCCCACCgcggcgaacgcg                                                 >  2:5716631/1‑95 (MQ=25)
                                                           aGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGcgccc                                            <  1:2848619/101‑1 (MQ=255)
                                                            ggCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGGGGCGCGAATGcgcccg                                           >  1:4625807/1‑101 (MQ=255)
                                                            ggCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGcgcccg                                           >  1:5369490/1‑101 (MQ=255)
                                                             gccccGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGcgcccgc                                          <  1:4551984/98‑1 (MQ=255)
                                                              cACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGcgcccgcc                                         >  2:360818/1‑101 (MQ=255)
                                                              cACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGcgcccgcc                                         >  2:2213595/1‑101 (MQ=255)
                                                              cACCGCCTTCCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGcgcccgcc                                         <  1:6229009/101‑1 (MQ=255)
                                                               ccccccTTCCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCa                                        <  2:5522672/97‑1 (MQ=255)
                                                                  gcatttCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATgg                                     >  2:6225726/4‑101 (MQ=255)
                                                                  gCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATgg                                     >  2:1226723/1‑101 (MQ=255)
                                                                  gCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGACGCGAATGCGCCCGCCATgg                                     >  1:5629787/1‑101 (MQ=255)
                                                                  cccTTCCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCCCCGCCC.CGGCGGCGCGAATGCGCCCGCCATgg                                     <  1:5822961/100‑1 (MQ=255)
                                                                   ctttcccccGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATggg                                    <  1:3837595/96‑1 (MQ=255)
                                                                   ccTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATggg                                    >  2:2533900/1‑101 (MQ=255)
                                                                   ccTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATggg                                    >  2:4411610/1‑101 (MQ=255)
                                                                    cTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATgggc                                   >  1:780153/1‑101 (MQ=255)
                                                                               cg.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGgcggggcgggggg                        >  1:2710046/1‑98 (MQ=255)
                                                                               cg.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGgcgc                        >  1:6758987/1‑101 (MQ=255)
                                                                                g.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGgggcc                       >  1:602822/1‑100 (MQ=255)
                                                                                g.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGgggcc                       >  1:4423093/1‑100 (MQ=255)
                                                                                   aTCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGAGGGGCGGGGGGAgg                     >  2:6274097/1‑101 (MQ=255)
                                                                                   aTCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCCCCCGCCATGGGCGGGGCGGgcgcccg                     >  1:2997068/1‑98 (MQ=255)
                                                                                     cGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGcc                   >  1:4893913/1‑101 (MQ=255)
                                                                                       cAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGgcggggcggggggcgggcgg                 >  2:6494788/1‑91 (MQ=255)
                                                                                        aaCAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.CA.g              >  1:590364/1‑101 (MQ=255)
                                                                                          caggggcgGCACGCCCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.GGGCGGGGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.ggg            <  1:3231501/96‑1 (MQ=17)
                                                                                          cAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCCAATGCGCCCGCCACGGGCGGGGCGGGGGGAGGGC.GC.ggg            >  2:3309167/1‑101 (MQ=18)
                                                                                           ggCCGCGGCATGACCGTTCCCGCAGGCGGGACAGAGGCC.GGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGc           <  2:4177725/100‑1 (MQ=255)
                                                                                             ccGCGGCATGCCCGTTTCCGAAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAAGGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGCCt         <  2:1126339/101‑1 (MQ=255)
                                                                                             ccGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGGGGGCGCAGGCC.GA.ggggcg         >  2:6942075/1‑98 (MQ=255)
                                                                                               ggggCATGACCGTTCCCGCAAGCGGGACAGAGGCC.GGCGCTCCGCCC.GGGGGGCGCGAAGGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGCCttt       <  1:4135853/99‑1 (MQ=17)
                                                                                               gcgcgaTGCCCTTTTCCGCAAGGGGGACAGAGGCC.TGCGCCCCCCCC.CGGCGGCGCGAATGCCCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGCCttt       <  1:2247955/96‑1 (MQ=14)
                                                                                                cgGCGTGACCGTTCCCGAATGCGGGAAGGAGGCC.TGCGCCCCGCCC.GGGGGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGCCTTTg      <  1:6447991/101‑1 (MQ=14)
                                                                                                cgGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGGCGGCC.GA.GGGCCCTTg      >  1:2336358/1‑101 (MQ=255)
                                                                                                   cATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCCGGG.GGGCCTTTggg    >  2:2021990/1‑101 (MQ=255)
                                                                                                   cATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GAGGGGCCTTTggg    >  1:1506991/1‑101 (MQ=255)
                                                                                                    aTGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGGGCAGGGC.GC.GGGCCCTTGGGGc  >  2:2075311/1‑101 (MQ=18)
                                                                                                     |                                                                                                       
TGACGGGCCGTCCGGCGCGGCTGAGCGAGGGGTGAGCGCGGTTTCGGGCGGATCAGGAAAGGCACCGCCTTTCCCCGCCCG.TATCGCAACAGCCGCGGCATGACCGTTTCCGCAAGCGGGACAGAGGCC.TGCGCTCCGCCC.CGGCGGCGCGAATGCGCCCGCCATGGGCGGGGCGGGCGCAGGCC.GA.GGGCCTTTGGGGC  >  NC_014034/1787241‑1787440

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG