BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,965,239	G→T	100%	intergenic (+7/+85)	bdhA → / ← RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,965,239	0	G→T	100.0%	398.1	114	intergenic (+7/+85)	bdhA/RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand): new base (70/44): ref base (0/0): total (70/44)

Only 100 of 131 total aligned reads displayed.


TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGGC.T.CCG..C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCC  >  NC_014034/1965141‑1965333


 ggggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGGGGCTGGACGGCGCCGTGAGAGATTtcgc                                                                                                     >  1:6182725/1‑101 (MQ=255)


 ggggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcgc                                                                                                     >  1:4032255/1‑101 (MQ=255)


 ggggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcgc                                                                                                     >  2:1398369/1‑101 (MQ=255)


  gggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGcc                                                                                                    <  2:5245365/101‑1 (MQ=255)


   ggggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCt                                                                                                   >  1:2337494/1‑101 (MQ=255)


    gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTtcgcacc                                                                                                  >  2:7000688/1‑98 (MQ=255)


    gggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTc                                                                                                  >  1:4971408/1‑101 (MQ=255)


     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                 <  1:4689912/101‑1 (MQ=255)


     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                 <  1:1347252/101‑1 (MQ=255)


     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                 <  2:1970080/101‑1 (MQ=255)


     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                 <  2:6636777/101‑1 (MQ=255)


     ggAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTCGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCg                                                                                                 >  1:4838344/1‑101 (MQ=255)


      gAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCgg                                                                                                <  1:4850762/101‑1 (MQ=255)


      gAACGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCgg                                                                                                >  2:1431107/1‑101 (MQ=255)


       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                               >  1:2464754/1‑101 (MQ=255)


       aaCGACGGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCggg                                                                                               >  1:4201090/1‑101 (MQ=255)


        acgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCgggg                                                                                              >  2:5957496/1‑101 (MQ=255)


         cgacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGc                                                                                             >  1:3695533/1‑101 (MQ=255)


          gacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGCCGGCGCCGTGAGCGATTTCGCCTCGGGGCa                                                                                            >  1:1232616/1‑101 (MQ=255)


          gacgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCa                                                                                            <  1:4074374/101‑1 (MQ=255)


           acgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCaa                                                                                           <  2:1634400/101‑1 (MQ=255)


            cgGTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAg                                                                                          <  2:6733344/101‑1 (MQ=255)


             ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                                         <  2:3141952/101‑1 (MQ=255)


             ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                                         <  1:2103398/101‑1 (MQ=255)


             ggTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGc                                                                                         <  2:4619457/101‑1 (MQ=255)


              gTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGcc                                                                                        >  2:4606734/1‑101 (MQ=255)


              gTCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGcc                                                                                        <  2:735617/101‑1 (MQ=255)


              gTCCTCCTGTGCAGCGAGGCGGCGGCCCAGATCACCGGCACGATGATTTCTGTGGGTGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCCAGcc                                                                                        <  2:2016994/101‑1 (MQ=255)


               tCTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGccc                                                                                       <  2:4270641/101‑1 (MQ=255)


                cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                                      <  1:6573065/101‑1 (MQ=255)


                cTTCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCg                                                                                      <  1:1624450/101‑1 (MQ=255)


                  tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAg                                                                                    <  1:827826/101‑1 (MQ=255)


                  tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAg                                                                                    <  1:5327856/101‑1 (MQ=255)


                  tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAg                                                                                    <  1:144332/101‑1 (MQ=255)


                  tCCTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAACCCGAg                                                                                    >  2:18006/1‑101 (MQ=255)


                   ccTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAgg                                                                                   <  1:683797/101‑1 (MQ=255)


                   ccTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAgg                                                                                   <  1:4405161/101‑1 (MQ=255)


                    cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGc                                                                                  <  1:2223465/101‑1 (MQ=255)


                    cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGc                                                                                  <  2:2568328/101‑1 (MQ=255)


                    cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGc                                                                                  <  2:6810323/101‑1 (MQ=255)


                    cTGTGTTCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGc                                                                                  <  2:4971156/101‑1 (MQ=255)


                     tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                 <  1:1460197/101‑1 (MQ=255)


                     tgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                 <  1:5848305/101‑1 (MQ=255)


                     cgtgttCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCg                                                                                 <  2:1354241/100‑1 (MQ=255)


                        gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                              >  2:897458/4‑101 (MQ=255)


                        gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                              <  2:726343/98‑1 (MQ=255)


                        gttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAcc                                                                              <  2:378757/98‑1 (MQ=255)


                         ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGCccc                                                                             >  1:193696/3‑101 (MQ=255)


                         ttccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGAccc                                                                             >  1:2919906/3‑101 (MQ=255)


                           ccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccgt                                                                           >  1:1661115/1‑100 (MQ=255)


                           ccGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.c                                                                          >  1:5677311/1‑101 (MQ=255)


                            cGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccgtc                                                                          <  2:1661054/101‑3 (MQ=255)


                            cGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.cc                                                                         >  2:4675156/1‑101 (MQ=255)


                             gAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACccg.ccc                                                                        >  2:237748/1‑100 (MQ=255)


                                  ggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGc                                                                 >  1:46366/1‑101 (MQ=255)


                                    cggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.c                                                             >  1:7106635/1‑101 (MQ=255)


                                     ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCCAGCCCGAGGCGACCCG.CC..CGGGGC.G.cc                                                            >  1:6606131/1‑101 (MQ=255)


                                     ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCG.GCGGGccc                                                               >  1:6195465/1‑99 (MQ=255)


                                     ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.G.cc                                                            >  1:3241168/1‑101 (MQ=255)


                                        gcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGggggcccccc                                                           >  1:5336827/1‑94 (MQ=38)


                                          aGATCACCTGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.C.CCC..c.ccc                                                    >  1:3578392/1‑101 (MQ=38)


                                              cACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.G.CCC..c.cccccgg                                                >  1:749567/1‑97 (MQ=37)


                                                  ggCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..CGGGGC.T.CCG..c.cccccgacccc                                            >  1:6959951/1‑93 (MQ=25)


                                                    cACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.G.CCC..c.ccccccccccccc                                          >  1:538156/1‑91 (MQ=25)


                                                     acgacgATTTCTGTGGATGGCGGCTGGTCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.TACCGC.C.CCCGGAccccga                                           >  2:3786489/1‑99 (MQ=25)


                                                     acgacgATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CCCGGACCCCCgc                                          >  2:3259254/1‑100 (MQ=37)


                                                        acgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.C.CCGCCC.CCCGGAccccccggg                                        >  1:4109494/1‑97 (MQ=21)


                                                           aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCGGGGGGGC.C.CCC..C.CCCGGCcccccccgggtt                                     >  2:3678283/1‑94 (MQ=21)


                                                             ttCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCCGGCCCCCGAGGGATTTc                                  >  2:2108575/1‑101 (MQ=25)


                                                              tgtggggggggcggcGGGACGGCGGTGGGAGAGATTCCGCCGGGGGGCACCCCGGGGGCGCCCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCa                                 <  2:1663383/92‑1 (MQ=9)


                                                               cTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC..GGGGGC.C.CCC..C.CCCCGGCCCCCCCGGGAATTCAg                                >  2:2444425/1‑101 (MQ=21)


                                                                 gtgGATGGCGGCTGTACGGCGCTGTGAGAGATTTCGCCTCGGGACAAGCCCGAGGCGACCCGCCC..GGGGGG.C.CCGC.C.CCCGGCCCCCCGGGGAGTTTCAgc                               >  1:6289584/1‑100 (MQ=16)


                                                                 gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGGAAGCCCGAGGCGACCCG.CC..CGGGGCGC.CCC..C.CCCCGGCCCCCGAGGGGGTTCAGGc                              >  2:6601945/1‑101 (MQ=19)


                                                                 gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCaagg                              >  2:3279957/1‑98 (MQ=25)


                                                                  tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCGCCC..GGGGGT.C.CCC..C.CCCCGGCCCCCCAGGGGTTTCAgggc                             >  1:7049404/1‑99 (MQ=16)


                                                                     aTGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.G.CCGC.C.CCCGGACCCCCCGGGGATTTTAGGCAAGa                          >  2:3130774/1‑101 (MQ=21)


                                                                      gggcggcGGGGCGGCGCGGGGAGAGTTTCCGCCGGGGGGCACCCCCGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCAGGCAAGAt                         <  1:5512047/100‑1 (MQ=9)


                                                                          ggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGGATTTCAGGCAAGATGaaa                     >  2:1148386/1‑101 (MQ=21)


                                                                          ggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCCGGCCCCCGAGGGATTTCAGGCAAGATGaaa                     >  2:6997681/1‑101 (MQ=21)


                                                                            ggggcgggCGCTGGGGGGGCTTCCGCCCCGGGCCACCCCGGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAaga                   <  1:5668433/95‑1 (MQ=2)


                                                                            cTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAaga                   >  1:3332131/1‑101 (MQ=21)


                                                                            cTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGGATTTCAGGCAAGATGAAaga                   >  1:1063692/1‑101 (MQ=21)


                                                                             ggggcGGCGCTGGGAGGGATTCCGCGCGGGGGCACGCCGGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAagag                  <  2:5528325/97‑1 (MQ=9)


                                                                              ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CCGGGGGGGC.C.CCC..C.CCCGGCCCCCCCGGGGATTTTCGGCAGAATGaagagg                  >  2:6055765/1‑97 (MQ=9)


                                                                              ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGGCCCCCCAGGGATTTCAGGCAAGATGAAAGAgg                 >  2:1966842/1‑101 (MQ=21)


                                                                              ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.C.CCGC.C.CCCGGCCCCCCGGGGGATTTCAGGCAAGATGAAAGAgg                 >  2:1135338/1‑101 (MQ=21)


                                                                                 cGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.C.CCGC.C.CCCCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAggggg              >  1:6749966/1‑100 (MQ=21)


                                                                                 cGGCGCTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAAAGGGgc              >  2:2052166/1‑101 (MQ=21)


                                                                                      cTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAgggggggcca         >  1:5379402/1‑95 (MQ=21)


                                                                                      cTGTGAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGGCCCCCGCGGGATTTCAGGCAAGATGAAAGAGGGGGGCTCa         >  2:1104549/1‑101 (MQ=21)


                                                                                       tgtgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.T.CCGCCC.CCCGGACCCCC.AGGGATTTCAGGGAAGATGAAAGAGGGgggcgccg        >  2:5590010/1‑97 (MQ=17)


                                                                                       tgtgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCCAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAg        >  1:2814987/1‑101 (MQ=21)


                                                                                       tgtgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.C.ACGC.C.CCCCGACCCCCGAGGTATTTCAGGCAAGATGCAAGAGGGgcgcggag        >  2:4226006/1‑97 (MQ=21)


                                                                                        gtgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CCCCGGCCCCCCAGGGATTTCAGGCAAGATGAAAAAGGGGGGCTCAgg       >  1:6693854/1‑101 (MQ=18)


                                                                                         tgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGG.G.CCGC.C.CCCGGACCCCCCAGGGCATTCAGGAAAAAAGAAAAAAGGGGGGTCCggc      >  2:6207568/1‑101 (MQ=9)


                                                                                         tgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGGATTTCAGGCCAGATGAAAGAGGGGCGCTCCggg      >  2:4330509/1‑100 (MQ=21)


                                                                                         tgAGAGATTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..CGGGGC.T.CCG..CGCCCGGACCCCCGAGGGATTTCACGCTAAGTGAAAGATGGGGGCTctcga      >  1:1201134/1‑97 (MQ=16)


                                                                                          gagagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcg     >  1:1122943/1‑101 (MQ=21)


                                                                                           agagaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..CGCGGC.T.CCGC.C.CCCGGCCACCCGCGGTTTTTGCGGGAAGATGAAAGAGGGGCGCTCCggccg    >  1:3070346/1‑99 (MQ=16)


                                                                                             agaTTTCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CCCGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcgggc  >  2:2265389/1‑99 (MQ=21)


TGGGGGGAACGACGGTCTTCCTGTGTGCCGAGGCGGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGGC.T.CCG..C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCC  >  NC_014034/1965141‑1965333

Base quality scores:


ATCG

< 3 ≤


ATCG

< 16 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG