Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,965,271 C→G 100% intergenic (+39/+53) bdhA → / ← RCAP_rcc01818 3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,965,2710C→G100.0% 143.5 58intergenic (+39/+53)bdhA/RCAP_rcc018183‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (42/16):  ref base (0/0):  total (42/16)

GGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GCGGGC.T.CCG..C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTT  >  NC_014034/1965175‑1965365
                                                                                                    |                                                                                                    
ggcggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGc                                                                                                  >  1:46366/1‑101 (MQ=255)
  cggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.c                                                                                              >  1:7106635/1‑101 (MQ=255)
   ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCCAG.CCCGAGGCGACCCG.CC..CGGGGC.G.cc                                                                                             >  1:6606131/1‑101 (MQ=255)
   ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.TCCGAGGCGACCCG.CC..GGgggagcc                                                                                               >  1:1515728/1‑97 (MQ=255)
   ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CCG.GCGGGccc                                                                                                >  1:6195465/1‑99 (MQ=255)
   ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.G.cc                                                                                             >  1:3241168/1‑101 (MQ=255)
      gcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGggggcccccc                                                                                            >  1:5336827/1‑94 (MQ=38)
        aGATCACCTGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.C.CCC..c.cc.c                                                                                    >  1:3578392/1‑101 (MQ=38)
          aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCG..c.cc.ccg                                                                                  >  2:3923592/1‑99 (MQ=255)
          aTCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.G.CCG..c.cc.ccc                                                                                  >  1:7067367/1‑99 (MQ=255)
            cACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCC..c.cc.cccgg                                                                                >  1:749567/1‑97 (MQ=37)
                ggCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..CGGGGC.T.CCG..c.cc.cccgacccc                                                                            >  1:6959951/1‑93 (MQ=25)
                  cACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCC..c.cc.ccccccccccc                                                                          >  1:538156/1‑91 (MQ=25)
                   acgacgATTTCTGTGGATGGCGGCTGGTCGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.TACCGC.C.CC.CGGAccccga                                                                           >  2:3786489/1‑99 (MQ=25)
                   acgacgATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CC.CGGACCCCCgc                                                                          >  2:3259254/1‑100 (MQ=37)
                     gttgtttgcTGTGGGGGGGGGGTGGGCGGCGCTGTGAGGTTTTCCGCCGGGGGGCAGG.CCCGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAgg                                                                        <  2:4813308/93‑1 (MQ=2)
                      acgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.CCGCCC.CC.CGGAccccccggg                                                                        >  1:4109494/1‑97 (MQ=21)
                         aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CCGGGGGGGC.C.CCC..C.CC.CGGCcccccccgggtt                                                                     >  2:3678283/1‑94 (MQ=21)
                         aTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.GCGC.C.CC.CCGGCCCCCGAgcacgt                                                                    >  2:2282712/1‑96 (MQ=21)
                           ttCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CCGGCCCCCGAGGGATTTc                                                                  >  2:2108575/1‑101 (MQ=25)
                            tgtggggggggcggcGGGACGGCGGTGGGAGAGATTCCGCCGGGGGGCACC.CCGGGGGCGCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCa                                                                 <  2:1663383/92‑1 (MQ=9)
                             cTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCGCCC..GGGGGC.C.CCC..C.CC.CCGGCCCCCCCGGGAATTCAg                                                                >  2:2444425/1‑101 (MQ=21)
                               gtgGATGGCGGCTGTACGGCGCTGTGAGAGATTTCGCCTCGGGACAAG.CCCGAGGCGACCCGCCC..GGGGGG.C.CCGC.C.CC.CGGCCCCCCGGGGAGTTTCAgc                                                               >  1:6289584/1‑100 (MQ=16)
                               gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGGAAG.CCCGAGGCGACCCG.CC..CGGGGCGC.CCC..C.CC.CCGGCCCCCGAGGGGGTTCAGGc                                                              >  2:6601945/1‑101 (MQ=19)
                               gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCGC.C.CC.CGGGCCCCCCCGGGATTTCAGGc                                                              >  2:6753129/1‑101 (MQ=21)
                               gtgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCaagg                                                              >  2:3279957/1‑98 (MQ=25)
                                tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCGCCC..GGGGGT.C.CCC..C.CC.CCGGCCCCCCAGGGGTTTCAgggc                                                             >  1:7049404/1‑99 (MQ=16)
                                tgGATGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGGCCCCCGAGGGATTTCAGGCa                                                             >  2:6582998/1‑101 (MQ=25)
                                   aTGGCGGCTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.G.CCGC.C.CC.CGGACCCCCCGGGGATTTTAGGCAAGa                                                          >  2:3130774/1‑101 (MQ=21)
                                    gggcggcGGGGCGGCGCGGGGAGAGTTTCCGCCGGGGGGCACC.CCCGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGAt                                                         <  1:5512047/100‑1 (MQ=9)
                                        ggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGGATTTCAGGCAAGATGaaa                                                     >  2:1148386/1‑101 (MQ=21)
                                        ggcTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CCGGCCCCCGAGGGATTTCAGGCAAGATGaaa                                                     >  2:6997681/1‑101 (MQ=21)
                                          ggggcgggCGCTGGGGGGGCTTCCGCCCCGGGCCACC.CCGGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAaga                                                   <  1:5668433/95‑1 (MQ=2)
                                          cTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAaga                                                   >  1:3332131/1‑101 (MQ=21)
                                          cTGGACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGGATTTCAGGCAAGATGAAaga                                                   >  1:1063692/1‑101 (MQ=21)
                                           ggggcGGCGCTGGGAGGGATTCCGCGCGGGGGCACG.CCGGGGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAagag                                                  <  2:5528325/97‑1 (MQ=9)
                                            ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CCGGGGGGGC.C.CCC..C.CC.CGGCCCCCCCGGGGATTTTCGGCAGAATGaagagg                                                  >  2:6055765/1‑97 (MQ=9)
                                            ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGGCCCCCCAGGGATTTCAGGCAAGATGAAAGAgg                                                 >  2:1966842/1‑101 (MQ=21)
                                            ggACGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.CCGC.C.CC.CGGCCCCCCGGGGGATTTCAGGCAAGATGAAAGAgg                                                 >  2:1135338/1‑101 (MQ=21)
                                               gggCGCGGGGAGAGGTGCCGCCGGGGGGGAAG.CCCGGGGCGCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGgc                                              <  1:5970606/100‑1 (MQ=12)
                                               cGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.C.CCGC.C.CC.CCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAggggg                                              >  1:6749966/1‑100 (MQ=21)
                                               cGGCGCTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGGATTTCAGGCAAGATGAAAAAGGGgc                                              >  2:2052166/1‑101 (MQ=21)
                                                    cTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CC.CGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAgggggggcca                                         >  1:5379402/1‑95 (MQ=21)
                                                    cTGTGAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGGCCCCCGCGGGATTTCAGGCAAGATGAAAGAGGGGGGCTCa                                         >  2:1104549/1‑101 (MQ=21)
                                                     tgtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGCCC.CC.CGGACCCCC.AGGGATTTCAGGGAAGATGAAAGAGGGgggcgccg                                        >  2:5590010/1‑97 (MQ=17)
                                                     tgtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCCAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAg                                        >  1:2814987/1‑101 (MQ=21)
                                                     tgtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.C.ACGC.C.CC.CCGACCCCCGAGGTATTTCAGGCAAGATGCAAGAGGGgcgcggag                                        >  2:4226006/1‑97 (MQ=21)
                                                      gtgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CC.CCGGCCCCCCAGGGATTTCAGGCAAGATGAAAAAGGGGGGCTCAgg                                       >  1:6693854/1‑101 (MQ=18)
                                                       tgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGG.G.CCGC.C.CC.CGGACCCCCCAGGGCATTCAGGAAAAAAGAAAAAAGGGGGGTCCggc                                      >  2:6207568/1‑101 (MQ=9)
                                                       tgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGGATTTCAGGCCAGATGAAAGAGGGGCGCTCCggg                                      >  2:4330509/1‑100 (MQ=21)
                                                       tgAGAGATTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..CGGGGC.T.CCG..CGCC.CGGACCCCCGAGGGATTTCACGCTAAGTGAAAGATGGGGGCTctcga                                      >  1:1201134/1‑97 (MQ=16)
                                                        gagagaTTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcg                                     >  1:1122943/1‑101 (MQ=21)
                                                         agagaTTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..CGCGGC.T.CCGC.C.CC.CGGCCACCCGCGGTTTTTGCGGGAAGATGAAAGAGGGGCGCTCCggccg                                    >  1:3070346/1‑99 (MQ=16)
                                                           agaTTTCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAggcgggc                                  >  2:2265389/1‑99 (MQ=21)
                                                               ttcgcCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGGCCCCCCAGGGATTTCCGGCAAGATGAAAGAGGGGCGCTCAGGGGGCcttct                              >  1:3203575/3‑101 (MQ=21)
                                                                cccccggggggCAAGCCCCGGGCCCCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCcttct                              <  2:5922423/94‑1 (MQ=18)
                                                                  gcCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GGGGGC.TCCCC..C.CC.CCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCttcttcc                           >  2:1645568/1‑100 (MQ=25)
                                                                     gggggcCAGG.CCCGCGGCCCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgtcg                        <  1:7001062/99‑1 (MQ=18)
                                                                       ggggCAAG.CCCGAGGCGACCCG.CC..GGGGGG.TCCCC..C.CC.CCGGCCCCCCAGGGATTTCAGGCAAAATGAAAGAAGGGGGCTCCGGCGGGCCTCTTCtccccc                      >  2:1956425/1‑101 (MQ=12)
                                                                       ggggCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCgccgcc                      >  2:3301308/1‑101 (MQ=255)
                                                                        gggCAAG.CCCGAGGCGACCCG.CCG.GGGGGC.C.CCC..C.CC.CCGGCCCCCCAGGGGTTTCCGGCAAGATGAAAGAGGGGCGCTCCGGCGGGCCTCTTCgccgccc                     >  2:5456036/1‑100 (MQ=19)
                                                                         ggCAAG.CCCGAGGCGACCCG.CC..GGGGGC.T.CCGCCC.CC.CGGACCCCC.AGGGATTTCAGGCAAGATGCAAGAGGGGCGCTCAGGCGGCCCTCTTCgccgccct                    >  1:6737006/1‑99 (MQ=21)
                                                                           gccccccagAGGCGCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTg                   <  1:4226201/93‑1 (MQ=21)
                                                                            aaG.CCCGAGGCGACCCG.CC..GGGGGG.T.CCGC.C.CC.CCGGCCCCCCCGGGATTTTCGGGCAGAAGGAAGAGGGGGGCGCCGGGGGGCCTCTTCGCCCCCCTGcc                 >  1:1672209/1‑101 (MQ=0)
                                                                               ccgcgcggCGCCCCG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCg               <  2:4632253/95‑1 (MQ=25)
                                                                                ccggcgcccccccG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCgg              <  2:7012487/91‑1 (MQ=25)
                                                                                 cggggcccccccG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgt             <  1:4330731/92‑1 (MQ=25)
                                                                                  cGAGGCGACCCG.CC..GGGGGC.TCCCC..C.CC.CCGACCCCCGAGGGATTTCAGGCCAGAAGAAAGAAGGGGGCCCAGGCGGGCCTCTTCTCCGCCCTGccccggg            >  2:2743228/1‑97 (MQ=12)
                                                                                   gcgcccccccG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtgt           <  2:2081807/94‑1 (MQ=25)
                                                                                   gAGGCGACCCG.CC..GGGGGC.TCCCC..C.CC.CCGACCCCCGAGGGATTTCAGGCAAGATGAAAGAGGGGCGCTCCGGCGGCCCTCCTCCGCGCCCTGCCCggggc           >  2:6781701/1‑98 (MQ=12)
                                                                                      cccccccG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc        <  1:7017370/97‑1 (MQ=25)
                                                                                         ccccG.CC..GGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg     <  1:4682194/100‑1 (MQ=255)
                                                                                         ccccG.CC..GGGGGC.T.CCG..C.CCGCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCggg     <  1:5607254/100‑1 (MQ=255)
                                                                                           ccg.cc..gGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct   <  1:60196/101‑1 (MQ=255)
                                                                                           ccg.cc..gGGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct   <  1:2490659/101‑1 (MQ=255)
                                                                                            cg.cc..ggGGGC.T.CCGC.C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGctt  <  2:4999147/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GGCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGCGCCTCGGGGCAAG.CCCGAGGCGACCCG.CC..GCGGGC.T.CCG..C.CC.CGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCTT  >  NC_014034/1965175‑1965365

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG