Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,976,894 C→G 94.0% intergenic (‑73/+7) fba ← / ← gap1 fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,976,8940C→G94.0% 46.8 ‑0.2 ‑0.2380intergenic (‑73/+7)fba/gap1fructose‑bisphosphate aldolase/glyceraldehyde‑3‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  new base (182/175):  ref base (11/12):  total (193/187)
Fisher's exact test strand distribution p-value = 8.32e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.65e-01

Only 100 of 388 total aligned reads displayed.

GCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976800‑1976990
                                                                                              |                                                                                                
gCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTtc                                                                                            >  2:1174113/1‑101 (MQ=255)
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGATTTCGCGGCCTTTTTCTTGGCCGAAATACGCAGGGGGTCCGGGGGCAGCGCCCCCGGCTTTtctc                                                                                          <  1:6368998/101‑1 (MQ=37)
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTtctc                                                                                          >  2:2483003/1‑101 (MQ=255)
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTtctc                                                                                          >  1:5503059/1‑101 (MQ=255)
  cGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTtctc                                                                                          <  1:2249962/101‑1 (MQ=255)
    aaGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAg                                                                                        >  1:6548865/1‑101 (MQ=255)
      gggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGAt                                                                                      >  2:6919478/1‑101 (MQ=255)
      gggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGAt                                                                                      <  2:5310656/101‑1 (MQ=255)
       ggTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATc                                                                                     >  2:756471/1‑101 (MQ=255)
        gTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCa                                                                                    >  1:5786913/1‑101 (MQ=255)
        gTGATCAGTCCCTTTTTGTTGGTTCCTTCAGTTCGCGACCTTTTTCTTGGCCAAAATAGGCCGGGGGTCCGGGGGCAGGGCCCCCGGCTTTTCTCAGATCa                                                                                    <  2:4404842/101‑1 (MQ=16)
           aTCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCt                                                                                 >  2:4536476/1‑101 (MQ=255)
            tcggtgcttttttCTGTGTTCTTTGTTTTCGCGCCTTTTTTCTGGCCCGAAATCGGCGGGGGGCCGGGGGGCGGCCCCCCGGGCTTTTCTCAGATCAGCtt                                                                                <  1:3369260/92‑1 (MQ=255)
             cAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTg                                                                               >  2:6715890/1‑101 (MQ=255)
              aGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGc                                                                              >  1:6091853/1‑101 (MQ=255)
               gTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGcc                                                                             >  1:6619679/1‑101 (MQ=255)
                tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            >  1:2544388/1‑101 (MQ=255)
                tGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGccc                                                                            <  1:3053962/101‑1 (MQ=255)
                  cctttttCTCAGTGCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  1:6997180/98‑1 (MQ=25)
                  ccATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCAt                                                                          <  2:2426009/101‑1 (MQ=255)
                   cATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATc                                                                         <  2:1898954/101‑1 (MQ=255)
                   cATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATc                                                                         >  2:5506167/1‑101 (MQ=255)
                   cATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATc                                                                         >  2:4303581/1‑101 (MQ=255)
                     ttttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATCCGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGc                                                                       <  1:5373367/101‑1 (MQ=37)
                      tttCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGcc                                                                      <  2:3101738/101‑1 (MQ=255)
                        tctcAGTTCCTTGATTTCGCGGCTTTTTTCTTGGCCAAAATCCGCGGGGGGTCCGGGGGCGGCCCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  1:1379739/101‑1 (MQ=21)
                        tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  1:850756/101‑1 (MQ=255)
                        tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    <  2:4010595/101‑1 (MQ=255)
                        tctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAc                                                                    >  2:2040014/1‑101 (MQ=255)
                         ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   >  2:5007533/1‑101 (MQ=255)
                         ctcAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCAGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCACCGCCAcg                                                                   >  1:3306998/1‑101 (MQ=37)
                         cccAGTTCCTTGGGTCCGGGGCTTTTTTCTGGGCCGAAATCCGCGGGGGGTCGGGGGGCAGCCCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcg                                                                   <  2:4705841/99‑1 (MQ=255)
                           cAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggc                                                                 >  1:1221106/1‑101 (MQ=255)
                           cAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCAcggc                                                                 >  1:3186246/1‑101 (MQ=255)
                              ttCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              >  1:4448940/1‑101 (MQ=255)
                              gtCCTTGAGTTCGCGGCCTTTTTCTTGCCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGt                                                              <  2:2612350/100‑1 (MQ=37)
                               tCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTa                                                             >  1:3575257/1‑101 (MQ=255)
                                ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTac                                                            >  2:6211931/1‑100 (MQ=255)
                                ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            >  2:1422140/1‑101 (MQ=255)
                                ccTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTAt                                                            >  1:1709063/1‑101 (MQ=255)
                                  ttGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCg                                                          <  2:4285648/101‑1 (MQ=255)
                                   tGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCgg                                                         >  2:3718111/1‑101 (MQ=255)
                                    gAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        <  1:5047536/101‑1 (MQ=255)
                                    gAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGc                                                        >  1:2047356/1‑101 (MQ=255)
                                     aGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       >  1:2666066/1‑101 (MQ=255)
                                     aGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGcc                                                       <  1:1975229/101‑1 (MQ=255)
                                      gctCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTTAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCa                                                      >  1:6327305/3‑101 (MQ=25)
                                      gTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGCATCGGccc                                                      >  1:4930764/1‑100 (MQ=25)
                                       ttCGGGGCCTTTTTCTGGGCCGAAATCCGCGGGGGGTCCGGGGGCAGCCCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCAt                                                     <  1:4636457/101‑1 (MQ=25)
                                         cgcgGCCTTTTTCTTGGCCGAAATCCGCCGGGGGTCGGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGc                                                   <  1:1868678/101‑1 (MQ=37)
                                          gcgGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCg                                                  >  1:6841191/1‑101 (MQ=255)
                                            ggCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGTCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:3244263/1‑101 (MQ=37)
                                            ggCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGt                                                >  1:1027581/1‑101 (MQ=255)
                                             gCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:3505777/101‑1 (MQ=255)
                                             gCCTTTTTCTGGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGtt                                               <  2:1625216/101‑1 (MQ=37)
                                              ccTTTTTCTTGGCCGAAATCCGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTc                                              <  2:664512/101‑1 (MQ=37)
                                               cTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  1:5661020/101‑1 (MQ=255)
                                               cTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCg                                             <  2:3311907/101‑1 (MQ=255)
                                                 ttttCTTGGCCGAAATCCGCGGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           <  1:1303159/101‑1 (MQ=25)
                                                 ttttCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           >  1:4166827/1‑101 (MQ=255)
                                                 ttttCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCgag                                           >  2:3898019/1‑101 (MQ=255)
                                                   ttCTTGGCCGAAATGCGCGGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         <  1:5236175/101‑1 (MQ=25)
                                                   ttCTTGGCCGAAATCCGCCGGGGGTCCGGGGGCACCCCCCCTGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         <  2:4110668/101‑1 (MQ=25)
                                                   ttCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  2:6323413/1‑101 (MQ=255)
                                                   ttCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  2:6049145/1‑101 (MQ=255)
                                                   ttCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGaa                                         >  1:109780/1‑101 (MQ=255)
                                                     cTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGc                                       <  1:405342/101‑1 (MQ=255)
                                                           cGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcatt                                 <  2:3209930/101‑1 (MQ=255)
                                                            gAAATACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattc                                >  2:1809007/1‑101 (MQ=255)
                                                             aaaTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               <  2:5091894/101‑1 (MQ=255)
                                                             aaaTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  2:1028713/1‑101 (MQ=255)
                                                             aaaTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattca                               >  1:6238746/1‑101 (MQ=255)
                                                              aaTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  1:4408057/1‑101 (MQ=255)
                                                              aaTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcat                              >  2:3262299/1‑101 (MQ=255)
                                                               aTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  2:6560990/1‑101 (MQ=255)
                                                               aTACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCcattcatt                             >  1:2854577/1‑101 (MQ=255)
                                                                tACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            >  2:2190967/1‑101 (MQ=255)
                                                                tACGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTg                            <  2:3836950/101‑1 (MQ=255)
                                                                 aCGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           >  2:5754497/1‑101 (MQ=255)
                                                                 aCGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           <  2:5649543/101‑1 (MQ=255)
                                                                 aCGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGt                           <  2:2525582/101‑1 (MQ=255)
                                                                  cGCCGGGGGTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTc                          <  2:6425246/101‑1 (MQ=255)
                                                                   gCCGGGGGTCGGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  2:5929147/101‑1 (MQ=21)
                                                                   gCCGGGGGTCCGGGGCCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCa                         <  2:2352119/101‑1 (MQ=21)
                                                                       ggggTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATAcc                     >  1:5904356/1‑101 (MQ=255)
                                                                        gggTCGGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  2:2847111/101‑1 (MQ=18)
                                                                        gggTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCa                    <  1:4347295/101‑1 (MQ=255)
                                                                         ggTCCGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCAc                   <  1:1174161/101‑1 (MQ=255)
                                                                           gccGGGGGCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGa                 <  1:6766575/100‑1 (MQ=255)
                                                                               ggggCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             <  1:3673498/101‑1 (MQ=255)
                                                                               ggggCAGCCCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAgg             <  1:4303803/101‑1 (MQ=18)
                                                                                gggCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGa            <  1:4194101/101‑1 (MQ=255)
                                                                                  gCAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATg          <  2:677378/101‑1 (MQ=255)
                                                                                   gaGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:701600/100‑1 (MQ=255)
                                                                                   cAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         >  1:7130143/1‑101 (MQ=255)
                                                                                   cAGCGCCCCCGGCTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGc         <  2:7118378/101‑1 (MQ=255)
                                                                                       cccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  2:5688110/100‑1 (MQ=255)
                                                                                       cccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGAcc    <  1:6425043/100‑1 (MQ=255)
                                                                                        ccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   <  1:2404297/101‑1 (MQ=255)
                                                                                        ccccGGC.TTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCa   <  1:943049/101‑1 (MQ=255)
                                                                                              |                                                                                                
GCCGAAGGGTGATCAGTGCCATTTTCTCAGTTCCTTGAGTTCGCGGCCTTTTTCTTGGCCGAAATACGCCGGGGGTCCGGGGGCAGCGCCCCGGCGTTTTCTCAGATCAGCTTGCCCATCGCCACGGCGGTATCGGCCATGCGGTTCGAGAAGCCCCATTCATTGTCATACCACGACAGGATGCGGACCAT  >  NC_014034/1976800‑1976990

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG