BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,990,962	T→A	100%	Y555F (TAC→TTC)	glgB ←	1,4‑alpha‑glucan branching protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,990,962	0	T→A	100.0%	1421.6	356	Y555F (TAC→TTC)	glgB	1,4‑alpha‑glucan branching protein
Reads supporting (aligned to +/- strand): new base (180/176): ref base (0/0): total (180/176)

Only 100 of 357 total aligned reads displayed.


GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059


gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                 <  1:5868579/101‑1 (MQ=255)


gAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAAc                                                                                                 <  1:1875522/101‑1 (MQ=255)


   ggTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCa                                                                                              >  2:2138643/1‑101 (MQ=255)


   ggTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCa                                                                                              <  1:2190921/101‑1 (MQ=255)


    gTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCAt                                                                                             >  1:3604103/1‑101 (MQ=255)


     tGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            >  2:3801886/1‑101 (MQ=255)


     ggCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATa                                                                                            <  2:6322262/100‑1 (MQ=255)


      gCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAg                                                                                           <  2:4626370/101‑1 (MQ=255)


       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          <  2:3970578/101‑1 (MQ=255)


       ccAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGa                                                                                          >  2:821076/1‑101 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         >  1:6485221/1‑101 (MQ=255)


        cAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGaa                                                                                         <  1:2523801/101‑1 (MQ=255)


         aGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAg                                                                                        <  1:729652/101‑1 (MQ=255)


         aGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAg                                                                                        >  2:1152225/1‑101 (MQ=255)


          gTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTCGCCCGGATGCGCCCATTGGAACGCATAGAAgg                                                                                       >  1:4496500/1‑101 (MQ=255)


            cAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGcc                                                                                     >  1:2764110/1‑101 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    <  1:5217871/101‑1 (MQ=255)


             aaGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGccc                                                                                    <  2:2811026/101‑1 (MQ=255)


              aGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCg                                                                                   >  1:6361176/1‑101 (MQ=255)


                 gCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAg                                                                                >  2:6234512/1‑101 (MQ=255)


                  cccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAgg                                                                               <  2:6534317/101‑1 (MQ=255)


                  cccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAgg                                                                               <  2:172150/101‑1 (MQ=255)


                   ccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGt                                                                              >  1:4803067/1‑101 (MQ=255)


                   ccccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGt                                                                              >  2:5932819/1‑101 (MQ=255)


                     ccTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                            >  2:270708/1‑101 (MQ=255)


                     ccTGATGCACCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTg                                                                            <  1:4622934/101‑1 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  2:1502358/1‑101 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  2:3055261/1‑101 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  2:4979438/1‑101 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  2:4459369/1‑101 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          <  2:4744525/101‑1 (MQ=255)


                       tGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGc                                                                          >  1:5416776/1‑101 (MQ=255)


                       tGAGGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCGTAGAAGGCCCGCAGGTTGGc                                                                          >  1:6847531/1‑101 (MQ=255)


                        gATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAGGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCg                                                                         >  2:3462248/1‑101 (MQ=255)


                           gcgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAt                                                                      >  1:5296450/1‑101 (MQ=255)


                            cgcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAtt                                                                     >  1:7119590/1‑101 (MQ=255)


                             gcCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCAATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAAttt                                                                    <  1:6318610/101‑1 (MQ=255)


                               ccATTCGCGCCCCTGCGCTAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCt                                                                  <  2:1965830/101‑1 (MQ=255)


                               ccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCt                                                                  >  2:3558445/1‑101 (MQ=255)


                               ccATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCt                                                                  <  1:85835/101‑1 (MQ=255)


                                cATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTg                                                                 <  2:3915897/101‑1 (MQ=255)


                                  ttCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCa                                                               <  1:1152273/101‑1 (MQ=255)


                                   tCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCaa                                                              >  1:1482762/1‑101 (MQ=255)


                                     gcgcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTGTGCAAcc                                                            >  2:4106051/1‑101 (MQ=255)


                                       gcCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCg                                                          >  2:5233555/1‑101 (MQ=255)


                                         cccTGCGCGAGCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtc                                                        <  2:2245510/101‑1 (MQ=255)


                                          ccTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCACCCCGtct                                                       >  2:5521721/1‑101 (MQ=255)


                                          ccTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGtct                                                       <  1:1678732/101‑1 (MQ=255)


                                            tGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTcc                                                     <  1:4038552/101‑1 (MQ=255)


                                            tGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTCCAACCCGTCTcc                                                     <  1:5052720/101‑1 (MQ=255)


                                             gcgcGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTccc                                                    <  2:6088612/101‑1 (MQ=255)


                                             gcgcGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTccc                                                    >  2:6459555/1‑101 (MQ=255)


                                             gcgcGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTccc                                                    <  1:4239242/101‑1 (MQ=255)


                                              cgcgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCg                                                   >  1:2656211/1‑101 (MQ=255)


                                                cgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGc                                                 >  1:2005729/1‑101 (MQ=255)


                                                cgAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGACCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGc                                                 >  1:5943883/1‑101 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               >  1:260736/1‑101 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               <  2:3603967/101‑1 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               <  1:284238/101‑1 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCAt                                               >  1:5660651/1‑101 (MQ=255)


                                                  aaCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCGCCCGGCAt                                               >  2:2293435/1‑101 (MQ=255)


                                                   aCTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATc                                              <  2:263113/101‑1 (MQ=255)


                                                    cTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCt                                             >  2:2953381/1‑101 (MQ=255)


                                                     tCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCtt                                            <  2:5108384/101‑1 (MQ=255)


                                                     tCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCtt                                            <  2:1426783/101‑1 (MQ=255)


                                                      ccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTg                                           <  1:2432324/101‑1 (MQ=255)


                                                      ccTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTg                                           <  2:3416932/101‑1 (MQ=255)


                                                         gCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGccc                                        >  1:4411705/1‑101 (MQ=255)


                                                         gCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGAAGGTTGGCGAATTTCTGCAACCCGGCTCCCGGCATCTTGccc                                        >  2:3303402/1‑101 (MQ=255)


                                                              aTGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCAt                                   >  2:4055986/1‑101 (MQ=255)


                                                               tGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATc                                  >  1:3064534/1‑101 (MQ=255)


                                                                gAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCg                                 <  1:321574/101‑1 (MQ=255)


                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAc                               >  1:2913886/1‑101 (MQ=255)


                                                                  agagCAGCTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGAc                               <  2:2223178/101‑1 (MQ=255)


                                                                     gcagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCCGCATCGAcccc                            >  2:6911642/1‑101 (MQ=255)


                                                                      cagcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCt                           <  1:6234831/101‑1 (MQ=255)


                                                                       agcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCtt                          >  2:57658/1‑101 (MQ=255)


                                                                        gcTTCTTGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTg                         >  1:3799710/1‑101 (MQ=255)


                                                                         cttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGc                        <  1:4329493/101‑1 (MQ=255)


                                                                          ttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGcc                       >  1:1048052/1‑101 (MQ=255)


                                                                          ttcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGcc                       <  2:4330581/101‑1 (MQ=255)


                                                                           tcttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCg                      <  2:4811194/101‑1 (MQ=255)


                                                                             ttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTg                    <  2:6966210/101‑1 (MQ=255)


                                                                             ttGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTg                    <  1:3674352/101‑1 (MQ=255)


                                                                              tGCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGa                   >  1:1351716/1‑101 (MQ=255)


                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  <  1:1944475/101‑1 (MQ=255)


                                                                               gCCCGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGaa                  <  1:1275198/101‑1 (MQ=255)


                                                                                cccGGATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAAc                 <  1:4725931/101‑1 (MQ=255)


                                                                                   ggATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAc              >  2:246463/1‑101 (MQ=255)


                                                                                    gATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAcc             >  1:3512699/1‑101 (MQ=255)


                                                                                    gATGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGAcc             >  1:2324707/1‑101 (MQ=255)


                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTTTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            >  2:6727054/1‑101 (MQ=255)


                                                                                     aTGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCt            >  1:612614/1‑101 (MQ=255)


                                                                                      tGCGCCCATTGGAACGCATAGAAGGCCCGCAGGTTTGCGAATTTCTGCAACCCGTCTGCCGGCATCTTGGCCAGCATCGACCCCTTGCCGTGAACGAccgc           >  2:616235/1‑99 (MQ=255)


                                                                                       gcgcCCATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCtcg          <  1:548880/101‑1 (MQ=255)


                                                                                            cATTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTg     >  1:5011273/1‑101 (MQ=255)


                                                                                             aTTGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGa    >  2:1919454/1‑101 (MQ=255)


                                                                                              ttGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGAc   >  2:1008688/1‑101 (MQ=255)


                                                                                              ttGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGAc   <  2:5048769/101‑1 (MQ=255)


                                                                                               tGGAACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACt  <  1:5629112/101‑1 (MQ=255)


GAAGGTGCCAGTCAAGGGCCCCCTGATGCGCCCATTCGCGCCCCTGCGCGAACTCCTGCCCCATGAAGAGCAGCTTCTTGCCCGGATGCGCCCATTGGTACGCATAGAAGGCCCGCAGGTTGGCGAATTTCTGCAACCCGTCTCCCGGCATCTTGCCCAGCATCGACCCCTTGCCGTGAACGACCTCGTCGTGACT  >  NC_014034/1990864‑1991059

Base quality scores:


ATCG

< 3 ≤


ATCG

< 25 ≤


ATCG

< 31 ≤


ATCG

< 35 ≤


ATCG

< 41 ≤


ATCG