Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,040,375 C→G 3.3% intergenic (‑123/‑265) RCAP_rcc01888 ← / → RCAP_rcc01889 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,040,3750C→G 3.3% 16.0 ‑1.6 ‑0.2334intergenic (‑123/‑265)RCAP_rcc01888/RCAP_rcc01889hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/7):  ref base (192/131):  total (196/138)
Fisher's exact test strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.47e-01

Only 100 of 374 total aligned reads displayed.

CGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.C.GCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040277‑2040476
                                                                                                  |                                                                                                       
cGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGggg                                                                                                      <  2:7051570/101‑1 (MQ=255)
cGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGggg                                                                                                      <  1:4043456/101‑1 (MQ=255)
 ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                     <  1:144415/101‑2 (MQ=255)
 ggCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggc                                                                                                     >  2:5099836/1‑100 (MQ=255)
  gCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCg                                                                                                   >  2:1643367/1‑101 (MQ=255)
    ctctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCGgggcctt                                                                                                  <  2:1859399/101‑5 (MQ=255)
      ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                               >  1:320931/1‑101 (MQ=255)
      ctGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATg                                                                                               <  2:5276715/101‑1 (MQ=255)
       tGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATgg                                                                                              >  1:5438441/1‑101 (MQ=255)
         ggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCt                                                                                            <  2:6792578/101‑1 (MQ=255)
         ggTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCt                                                                                            >  1:2789978/1‑101 (MQ=255)
           ttGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTa                                                                                          >  1:3359683/1‑101 (MQ=255)
            tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                         <  1:6903458/101‑1 (MQ=255)
            tGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAc                                                                                         >  2:2249328/1‑101 (MQ=255)
              ggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                       >  1:1179874/1‑101 (MQ=255)
              ggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                       <  2:1385016/101‑1 (MQ=255)
              ggATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                       <  1:766799/101‑1 (MQ=255)
              gcaTGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgc                                                                                       >  1:4256257/3‑101 (MQ=255)
               gATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTAcgcg                                                                                      >  1:4328601/1‑101 (MQ=255)
                  ggAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTa                                                                                   >  1:2458957/1‑101 (MQ=255)
                    aaaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATg                                                                                 <  2:6338522/101‑1 (MQ=255)
                     aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                                >  2:5828050/1‑101 (MQ=255)
                     aaaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATgg                                                                                <  2:5388299/101‑1 (MQ=255)
                      aaaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgc                                                                               >  1:7079744/1‑101 (MQ=255)
                       aaGACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGgcg                                                                              >  1:1837060/1‑101 (MQ=255)
                         gACCGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGcgcg                                                                            >  1:5921794/1‑101 (MQ=255)
                           ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                          <  2:5434603/101‑1 (MQ=255)
                           ccGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTg                                                                          <  2:3276307/101‑1 (MQ=255)
                            cGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGc                                                                         <  1:6019854/101‑1 (MQ=255)
                            cGGAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGc                                                                         <  2:1952611/101‑1 (MQ=255)
                             ggAACGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGcc                                                                        >  2:5561793/1‑101 (MQ=255)
                                 cGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAg                                                                    >  1:3856141/1‑101 (MQ=255)
                                 cGGCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAg                                                                    <  1:6129705/101‑1 (MQ=255)
                                  ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                   <  1:4707452/101‑1 (MQ=255)
                                  ggCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGa                                                                   >  1:3001982/1‑101 (MQ=255)
                                   gCCAGGGGCCAGACGCAGGCGT.GGGGGATCCGGGCGAAGGGCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGAc                                                                  <  1:2282980/101‑1 (MQ=255)
                                          gCCAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGcc                                                           <  2:2768538/101‑1 (MQ=255)
                                            cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                         <  2:4738216/101‑1 (MQ=255)
                                            cAGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGc                                                         >  1:1389291/1‑101 (MQ=255)
                                             aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCa                                                        <  1:662587/101‑1 (MQ=255)
                                             aGACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCa                                                        >  1:4451722/1‑101 (MQ=255)
                                              gACGCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCaa                                                       <  1:2730363/101‑1 (MQ=255)
                                                 gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                                    >  2:30396/1‑101 (MQ=255)
                                                 gCAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAgg                                                    <  2:3464499/101‑1 (MQ=255)
                                                  cAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.c                                                  >  2:3757468/1‑101 (MQ=255)
                                                  cAGGCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.c                                                  >  1:2899281/1‑101 (MQ=255)
                                                    ggCGT.GGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccg                                                >  2:6161055/1‑99 (MQ=255)
                                                       cgtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGg.ccgcg                                              >  1:6328631/4‑97 (MQ=255)
                                                        gtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcgc                                             >  1:6251118/3‑96 (MQ=255)
                                                        gtgggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGg.ccgcgc                                             >  1:6840959/3‑96 (MQ=255)
                                                        gtgggggATCCGGT.GAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGcc                                            >  2:6823062/3‑101 (MQ=255)
                                                          gggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCa                                           <  2:5945060/101‑1 (MQ=255)
                                                           ggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCAt                                          >  2:4497443/1‑101 (MQ=255)
                                                           ggggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGAC.GCGCCAt                                          >  1:2535111/1‑101 (MQ=255)
                                                            gggATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATa                                         >  1:3064132/1‑101 (MQ=255)
                                                               aTCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGt                                      <  2:2861354/101‑1 (MQ=255)
                                                               aTCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGt                                      <  2:4438476/101‑1 (MQ=255)
                                                                tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTg                                     <  2:889940/101‑1 (MQ=255)
                                                                tCCGGTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTg                                     <  2:5034635/101‑1 (MQ=255)
                                                                 ccGGTCGAAGGCCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGTCATAAGTgc                                    >  2:6582652/1‑100 (MQ=255)
                                                                   ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGc                                  >  1:2661294/1‑101 (MQ=255)
                                                                   ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGc                                  >  2:3576020/1‑101 (MQ=255)
                                                                   ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGc                                  >  2:4939362/1‑101 (MQ=255)
                                                                   ggTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGAGGCCGCAAAGGCC.GCGCCATAAGTGAGc                                  >  2:6496775/1‑101 (MQ=255)
                                                                    gTCGAAGGTCAGCCGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGAGAGTGCCACAGACGGAGAACGAAAGGG.CAGCGCCATCAACGAGcc                                 >  2:2110194/1‑101 (MQ=255)
                                                                    gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGcc                                 <  2:3170143/101‑1 (MQ=255)
                                                                    gTCGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGcc                                 >  1:48808/1‑101 (MQ=255)
                                                                      cGAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCa                               <  2:6630362/101‑1 (MQ=255)
                                                                       gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              >  1:3762448/1‑101 (MQ=255)
                                                                       gAAGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAc                              >  2:3855663/1‑101 (MQ=255)
                                                                        aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAcc                             <  1:2120147/101‑1 (MQ=255)
                                                                        aaGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAcc                             <  1:5828352/101‑1 (MQ=255)
                                                                         aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccg                            >  2:784189/1‑100 (MQ=255)
                                                                         aGGTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccg                            >  2:4050797/1‑100 (MQ=255)
                                                                          ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccgg                           >  2:3379483/1‑99 (MQ=255)
                                                                          ggTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccgg                           <  2:4256062/101‑3 (MQ=255)
                                                                           gTCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccggt                          >  2:2520174/1‑98 (MQ=255)
                                                                            tCAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCAccggtt                         <  2:4669595/101‑5 (MQ=255)
                                                                             cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTc                       <  2:1172453/101‑1 (MQ=255)
                                                                             cAGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTc                       <  1:1301444/101‑1 (MQ=255)
                                                                              aGACGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCg                      <  2:482142/101‑1 (MQ=255)
                                                                                aCGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGa                    <  1:6671053/101‑1 (MQ=255)
                                                                                aCGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGa                    <  2:1118037/101‑1 (MQ=255)
                                                                                 cGTTGTGCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAg                   <  1:4921071/101‑1 (MQ=255)
                                                                                    tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                >  1:56862/1‑99 (MQ=255)
                                                                                    tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                >  1:6751993/1‑99 (MQ=255)
                                                                                    tgtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                >  2:2957239/1‑99 (MQ=255)
                                                                                    tgtgCAAAAG.AACCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggtt                >  2:5651077/1‑99 (MQ=255)
                                                                                     gtgCAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGAggttg               <  1:6399624/101‑4 (MQ=255)
                                                                                        cAAAAGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGAt           <  1:1584070/101‑1 (MQ=255)
                                                                                         aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg          >  2:5737910/1‑101 (MQ=255)
                                                                                         aaaaGCA.CCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATg          <  2:2423933/101‑1 (MQ=255)
                                                                                              gcaccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATa      <  2:1425698/98‑1 (MQ=255)
                                                                                               caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     <  1:3571496/99‑1 (MQ=255)
                                                                                               caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     <  2:4677149/99‑1 (MQ=255)
                                                                                               caccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaa     >  2:2648248/3‑101 (MQ=255)
                                                                                                accGGGGCGGATGGCTTACGCGGTATGGCGCTTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaaa    <  1:563955/100‑1 (MQ=255)
                                                                                                accGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATaaa    <  1:4645859/100‑1 (MQ=255)
                                                                                                 ccGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAATACGGCGGCCGCAAAGGCC.GCGCCATAAGTGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt   <  1:5100096/101‑1 (MQ=255)
                                                                                                 ccGGGGCGGAGGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGGCC.GCGCCATAAGGGAGCCCA.CCGGTTCGGA.GGTTGGATGCATAAAt   <  1:1074261/101‑1 (MQ=255)
                                                                                                  |                                                                                                       
CGGCCTCTGGGTTGGGATGGAAAAAGACCGGAACGGCCAGGGGCCAGACGCAGGCGTGGGGGGATCCGGTCGAAGGTCAGACGTTGTGCAAAAGCACCCGGGGCGGATGGCTTACGCGGTATGGCGCGTGCCAAAGACGGCGGCCGCAAAGG.C.GCGCCATAAGTGAGCCCACCCGGTTCGGAGGGTTGGATGCATAAATC  >  NC_014034/2040277‑2040476

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG