BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,123,404	A→G	1.7%	P256P (CCT→CCC)	wza ←	polysaccharide export protein Wza

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,123,404	0	A→G	1.7%	4.1 ‑1.4 ‑0.1	341	P256P (CCT→CCC)	wza	polysaccharide export protein Wza
Reads supporting (aligned to +/- strand): new base (4/2): ref base (138/196): total (143/198)
Fisher's exact test strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.58e-01

Only 100 of 365 total aligned reads displayed.


AAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATG  >  NC_014034/2123306‑2123503


aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                    >  1:5957176/1‑101 (MQ=255)


aaGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGgg                                                                                                    >  2:1352288/1‑101 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                   <  1:5274859/101‑2 (MQ=255)


 aGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAGggc                                                                                                   >  1:3104895/1‑100 (MQ=255)


  gATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                                 <  2:2195543/101‑1 (MQ=255)


  gATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                                 >  1:1091439/1‑101 (MQ=255)


  gATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCt                                                                                                 >  2:3236687/1‑101 (MQ=255)


   aTGCCGCGGGCACCGGCCTTGTCCTCGGGCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGAGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCtt                                                                                                >  1:5065398/1‑101 (MQ=255)


      ccGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAc                                                                                             <  1:355048/101‑1 (MQ=255)


       cgcgGGCATCGGCCCTGTCCTCGGCCAGACCGCCGACGCGGGTGATCTCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcg                                                                                            <  1:3289112/101‑2 (MQ=255)


        gcgGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcga                                                                                           <  2:3646820/101‑3 (MQ=255)


         cgggtgtCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATTTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgat                                                                                          <  2:593793/95‑4 (MQ=255)


          gggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCCTCAGATCGATCGTCG.AGGGCTTGAcgatc                                                                                         >  1:3761753/1‑97 (MQ=255)


           ggCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTCCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatct                                                                                        <  1:6681171/101‑6 (MQ=255)


             cATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctgg                                                                                      <  2:388070/101‑8 (MQ=255)


              aTCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggc                                                                                     <  2:4881212/101‑9 (MQ=255)


               tCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTCTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggcc                                                                                    <  2:3444695/101‑10 (MQ=255)


                cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                                   <  2:1213825/101‑11 (MQ=255)


                cGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAcgatctggccc                                                                                   <  1:5876632/101‑11 (MQ=255)


                         ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAg                                                                          <  2:126251/101‑1 (MQ=255)


                          cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAgg                                                                         <  1:621676/101‑1 (MQ=255)


                          cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAgg                                                                         >  2:5283141/1‑101 (MQ=255)


                          cTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAgg                                                                         <  1:4049016/101‑1 (MQ=255)


                            cGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcc                                                                       <  2:4708341/101‑1 (MQ=255)


                             tctcAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcct                                                                      <  1:6894280/98‑1 (MQ=255)


                             ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcct                                                                      <  2:6466982/101‑1 (MQ=255)


                             ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGccc                                                                      >  1:1001050/1‑100 (MQ=255)


                              gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGGGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCGCGCAGATAGGcctc                                                                     >  1:7122015/1‑101 (MQ=255)


                              gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctc                                                                     <  1:2553574/101‑1 (MQ=255)


                                cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGcctcct                                                                   <  1:722144/101‑1 (MQ=255)


                                  gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                 <  2:68626/101‑1 (MQ=255)


                                  gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGc                                                                 <  2:764006/101‑1 (MQ=255)


                                    ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                               <  1:1655556/101‑1 (MQ=255)


                                    ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                               <  1:1377191/101‑1 (MQ=255)


                                    ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAc                                                               <  1:2588853/101‑1 (MQ=255)


                                     cgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCAcc                                                              <  1:966937/101‑1 (MQ=255)


                                      gccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCg                                                             <  2:6229887/101‑1 (MQ=255)


                                             cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                      <  2:2985833/101‑1 (MQ=255)


                                                gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                   >  2:832333/1‑101 (MQ=255)


                                                 tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                  >  1:232448/1‑101 (MQ=255)


                                                   aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                                <  2:3743372/101‑1 (MQ=255)


                                                    tCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACg                                               <  1:5212238/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATTTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             <  2:1492481/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             <  2:1613777/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             <  2:5105399/101‑1 (MQ=255)


                                                      gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                             >  1:5368490/1‑101 (MQ=255)


                                                         tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                          >  1:3501255/1‑101 (MQ=255)


                                                          gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                         <  2:4367209/101‑1 (MQ=255)


                                                          gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCCGCACCGTCTGCCGCCCGCCGCTgacg                                         >  1:2463185/1‑101 (MQ=255)


                                                           ggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacga                                        >  2:4176140/1‑101 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                     >  1:469203/1‑101 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                     >  2:7024033/1‑101 (MQ=255)


                                                              cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                     <  2:5368218/101‑1 (MQ=255)


                                                               aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                    <  1:4751123/101‑1 (MQ=255)


                                                               aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                    >  2:2070286/1‑101 (MQ=255)


                                                               aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                    <  1:6418792/101‑1 (MQ=255)


                                                                gggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCg                                   <  2:5627747/101‑1 (MQ=255)


                                                                 ggTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGc                                  >  2:2403535/1‑101 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                 <  2:1035293/101‑1 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                 >  1:5439942/1‑101 (MQ=255)


                                                                  gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                 >  1:5774171/1‑101 (MQ=255)


                                                                   tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                >  1:6326121/1‑101 (MQ=255)


                                                                   tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                >  1:3031032/1‑101 (MQ=255)


                                                                    gATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGt                               <  2:6301463/101‑1 (MQ=255)


                                                                     aTGTTTTCCAGCGTCAGATCGGTCGTCG.AGGGCTTGACGATCTGCCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtt                              <  2:411853/101‑1 (MQ=255)


                                                                       gTTTTCTAGCGGCAGAGCGGTCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                            <  2:5880886/101‑1 (MQ=255)


                                                                       gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                            <  2:5789214/101‑1 (MQ=255)


                                                                        ccgatctgCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTg                           <  2:4473205/94‑1 (MQ=255)


                                                                         tttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgc                          >  2:3085196/1‑101 (MQ=255)


                                                                          ttCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgca                         >  2:909910/1‑101 (MQ=255)


                                                                           tCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcag                        <  1:318208/101‑1 (MQ=255)


                                                                            ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                       >  2:1147681/1‑101 (MQ=255)


                                                                            ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                       >  1:2615826/1‑101 (MQ=255)


                                                                             cAGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagca                      <  2:328212/101‑1 (MQ=255)


                                                                              aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAc                     <  2:4985506/101‑1 (MQ=255)


                                                                              aGCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGACGTTTTGCAGCAc                     >  2:6395338/1‑101 (MQ=255)


                                                                               gCGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc                    <  1:3241708/101‑1 (MQ=255)


                                                                                cGTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg                   >  1:5201346/1‑101 (MQ=255)


                                                                                 gTCAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCgg                  >  1:4419728/1‑101 (MQ=255)


                                                                                   cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggtt                <  1:4255962/101‑3 (MQ=255)


                                                                                   cAGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                >  2:6165744/1‑101 (MQ=255)


                                                                                    aGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt               <  1:1825161/101‑1 (MQ=255)


                                                                                    aGATCGATCGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt               <  2:663071/101‑1 (MQ=255)


                                                                                     gatcgatcGTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg              <  1:6974127/101‑1 (MQ=255)


                                                                                      atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAGGCTGACGACAACCCCGTTTTCCAGCCCCggggtat             >  2:6946140/1‑97 (MQ=255)


                                                                                      atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt             <  1:831201/101‑1 (MQ=255)


                                                                                      atcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgt             >  1:4604613/1‑101 (MQ=255)


                                                                                       tcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt            <  1:6495903/101‑1 (MQ=255)


                                                                                       tcgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt            >  1:1990883/1‑101 (MQ=255)


                                                                                        tgatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg           <  1:1861418/100‑1 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttga          >  2:1861346/1‑100 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          >  1:3627091/1‑101 (MQ=255)


                                                                                         gatcgTCG.AGGGCTTGACGATATGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg          <  1:3006909/101‑1 (MQ=255)


                                                                                          atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc         >  2:1683877/1‑101 (MQ=255)


                                                                                          atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgc         <  2:1967058/101‑1 (MQ=255)


                                                                                          atcgTCG.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGAAATCGCCGGTTTTCAGCACAgggcgggtggc         >  2:2285056/1‑93 (MQ=255)


                                                                                           tcgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcg        <  2:253823/101‑1 (MQ=255)


                                                                                            cgtcg.AGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc       >  1:2821827/1‑101 (MQ=255)


                                                                                              gtcgaGGGCTTGACGATCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGcc      >  1:1236793/5‑101 (MQ=255)


                                                                                                 gaGGGCTTGACGGTCTGGCCCAGCAGATTGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGAGATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt   >  1:6270340/2‑101 (MQ=255)


AAGATGCCGCGGGCATCGGCCTTGTCCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATTTGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATG  >  NC_014034/2123306‑2123503

Base quality scores:


ATCG

< 3 ≤


ATCG

< 21 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG