Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,123,424 A→G 100% M250M (TTG→CTG)  wza ← polysaccharide export protein Wza

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,123,4240A→G100.0% 1353.1 356M250M (TTG→CTG) wzapolysaccharide export protein Wza
Reads supporting (aligned to +/- strand):  new base (152/204):  ref base (0/0):  total (152/204)

Only 100 of 362 total aligned reads displayed.

CCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATT.TGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGCC  >  NC_014034/2123331‑2123521
                                                                                               |                                                                                                 
ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAg                                                                                            <  2:5911777/101‑1 (MQ=255)
ccTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAg                                                                                            <  2:126251/101‑1 (MQ=255)
ccTCGGCCAGACAGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAg                                                                                            >  1:5135046/1‑101 (MQ=255)
    tctcAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGcct                                                                                        <  1:6894280/98‑1 (MQ=255)
    ggCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGccc                                                                                        >  1:1001050/1‑100 (MQ=255)
     gCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGcctc                                                                                       <  2:5176488/101‑1 (MQ=255)
      ccAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGcctcc                                                                                      >  1:907249/1‑101 (MQ=255)
       cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGcctcct                                                                                     <  2:1018559/101‑1 (MQ=255)
       cAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGcctcct                                                                                     <  1:722144/101‑1 (MQ=255)
        aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTg                                                                                    >  1:2562829/1‑101 (MQ=255)
        aGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTg                                                                                    >  1:5495347/1‑101 (MQ=255)
         gACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGc                                                                                   <  2:68626/101‑1 (MQ=255)
           ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  1:2588853/101‑1 (MQ=255)
           ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  2:1501117/101‑1 (MQ=255)
           ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  1:1377191/101‑1 (MQ=255)
           ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  1:3318905/101‑1 (MQ=255)
           ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  1:1655556/101‑1 (MQ=255)
           ccgccgACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCAc                                                                                 <  1:1855908/101‑1 (MQ=255)
                   gcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTgcc                                                                         <  2:2382000/101‑1 (MQ=255)
                   gcgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTgcc                                                                         <  1:3165009/101‑1 (MQ=255)
                    cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                                        <  1:2496558/101‑1 (MQ=255)
                    cgGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccg                                                                        >  1:6301787/1‑101 (MQ=255)
                     gggTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTgccgc                                                                       >  2:499853/1‑101 (MQ=255)
                       gTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGccc                                                                     >  2:1825097/1‑101 (MQ=255)
                        tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                                    <  2:1195316/101‑1 (MQ=255)
                        tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                                    >  1:232448/1‑101 (MQ=255)
                        tGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCg                                                                    >  1:307781/1‑101 (MQ=255)
                         gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCgc                                                                   >  2:4903603/1‑100 (MQ=255)
                         gATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGa                                                                   <  1:645054/101‑1 (MQ=255)
                          aTCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGAc                                                                  >  1:3011214/1‑101 (MQ=255)
                             gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                                               <  1:3523199/101‑1 (MQ=255)
                             gCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCt                                                               >  1:5368490/1‑101 (MQ=255)
                              ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                              >  1:3746930/1‑101 (MQ=255)
                              ccTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTg                                                              <  2:4701378/101‑1 (MQ=255)
                               cTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGCCTCCCGCCCGACGCTgc                                                             >  1:10776/1‑100 (MQ=255)
                                tGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgac                                                            >  2:1895759/1‑101 (MQ=255)
                                 gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                                           >  2:676229/1‑101 (MQ=255)
                                 gggTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGAC.GTTCTGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacg                                                           >  2:209330/1‑101 (MQ=255)
                                   gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         >  2:5629376/1‑101 (MQ=255)
                                   gTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTgacgac                                                         <  1:6066232/101‑1 (MQ=255)
                                    tCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACa                                                        <  2:3324205/101‑1 (MQ=255)
                                     cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                                       >  2:4131600/1‑101 (MQ=255)
                                     cAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACAt                                                       >  2:7128612/1‑101 (MQ=255)
                                      aGGGTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATc                                                      <  1:5235027/101‑1 (MQ=255)
                                         gTGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGcc                                                   <  1:2944124/101‑1 (MQ=255)
                                          tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                                  <  1:6242899/101‑1 (MQ=255)
                                          tGATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCg                                                  >  1:2085371/1‑101 (MQ=255)
                                           gATGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGt                                                 <  2:6301463/101‑1 (MQ=255)
                                            aTGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtt                                                >  2:2520304/1‑101 (MQ=255)
                                            aTGTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtt                                                <  2:1477292/101‑1 (MQ=255)
                                              gTTTTCCAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGtttt                                              <  2:5789214/101‑1 (MQ=255)
                                                   ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                                         <  2:907225/101‑1 (MQ=255)
                                                   ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                                         >  2:6991784/1‑101 (MQ=255)
                                                   ccAGCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgcagc                                         >  1:2615826/1‑101 (MQ=255)
                                                      gCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTgagatcgg                                      >  1:4473444/1‑94 (MQ=255)
                                                      gCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc                                      <  1:3241708/101‑1 (MQ=255)
                                                      gCGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCAcc                                      <  1:584888/101‑1 (MQ=255)
                                                       cGTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCg                                     <  1:4699451/101‑1 (MQ=255)
                                                        gTCAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACCTCGCCGTTTTGCAGCACCgg                                    >  2:3926545/1‑101 (MQ=255)
                                                          cAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCggtt                                  <  1:4255962/101‑3 (MQ=255)
                                                          cAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                                  >  1:4657094/1‑101 (MQ=255)
                                                          cAGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgt                                  >  2:6165744/1‑101 (MQ=255)
                                                           aGATCGATCGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgtt                                 <  1:1825161/101‑1 (MQ=255)
                                                            gatcgatcGTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttg                                <  1:6974127/101‑1 (MQ=255)
                                                              tcgatcgTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGgttgtt                              >  2:1626052/1‑101 (MQ=255)
                                                               cgatcgTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg                             >  2:4404415/1‑101 (MQ=255)
                                                               cgatcgTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGttgttg                             >  2:4374422/1‑101 (MQ=255)
                                                                gatcgTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg                            <  2:6420866/101‑1 (MQ=255)
                                                                gatcgTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTgg                            <  1:3985033/101‑1 (MQ=255)
                                                                 atcgTCG.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGAAATCGCCGGTTTTCAGCACAgggcgggtggc                           >  2:2285056/1‑93 (MQ=255)
                                                                   cgtcg.AGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGgcgc                         >  1:711108/1‑101 (MQ=255)
                                                                     gtcgaGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGcc                        >  1:1236793/5‑101 (MQ=255)
                                                                      tcgaGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCg                       >  1:2806702/4‑101 (MQ=255)
                                                                        gaGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGAt                     >  1:6658009/2‑101 (MQ=255)
                                                                         ggggCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGATGGCGCCGTTg                    <  2:315468/100‑1 (MQ=255)
                                                                         aGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATg                    <  1:5205843/101‑1 (MQ=255)
                                                                         aGGGCTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATg                    >  2:6557414/1‑101 (MQ=255)
                                                                             cTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt                >  1:4431712/1‑101 (MQ=255)
                                                                             cTTGACGATC.TGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCt                <  1:5719764/101‑1 (MQ=255)
                                                                               ttgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTg               >  2:3734794/10‑101 (MQ=255)
                                                                               ttgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTg               <  2:4720483/92‑1 (MQ=255)
                                                                               ttgacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTg               <  1:578445/92‑1 (MQ=255)
                                                                                 gacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGtt             <  1:487048/94‑1 (MQ=255)
                                                                                 gacgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGtt             <  2:1050808/94‑1 (MQ=255)
                                                                                  acgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTc            <  2:3647425/95‑1 (MQ=255)
                                                                                   cgatctGGCCCAGCAGATCGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTcc           <  2:5977738/96‑1 (MQ=255)
                                                                                   cgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTcc           <  2:5102287/96‑1 (MQ=255)
                                                                                   cgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTcc           <  2:1313418/96‑1 (MQ=255)
                                                                                   cgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTcc           >  2:4261319/6‑101 (MQ=255)
                                                                                   cgatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTcc           <  1:1987417/96‑1 (MQ=255)
                                                                                    gatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCa          <  2:5296380/97‑1 (MQ=255)
                                                                                    gatctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCa          >  2:300732/5‑101 (MQ=255)
                                                                                     atctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAg         <  1:4337794/98‑1 (MQ=255)
                                                                                      tctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGa        <  1:639924/99‑1 (MQ=255)
                                                                                      tctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGa        <  1:4131795/99‑1 (MQ=255)
                                                                                       ctGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaa       >  2:5436599/2‑101 (MQ=255)
                                                                                        tGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaaa      >  2:281227/1‑101 (MQ=255)
                                                                                        tGGCCCAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGaaa      <  2:1988777/101‑1 (MQ=255)
                                                                                         ggcccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAg     >  1:3614092/4‑101 (MQ=255)
                                                                                         ggcccAGCAGATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAg     >  2:783432/4‑101 (MQ=255)
                                                                                               |                                                                                                 
CCTCGGCCAGACCGCCGACGCGGGTGATCGCCTGGGTCAGGGTGATGTTTTCCAGCGTCAGATCGATCGTCGAAGGGCTTGAC.ATT.TGGGCCAACAAATAGGCCTCCTGCACCGTCTGCCGCCCGACGCTGACGACATCGCCGTTTTGCAGCACCGGGTTGTTGGCGCCGATGCCCTGTTCCAGAAAGGCC  >  NC_014034/2123331‑2123521

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG